##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780452_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 95622 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.578820773462173 34.0 23.0 34.0 12.0 34.0 2 29.786576310890798 34.0 31.0 34.0 12.0 34.0 3 29.69870950199745 34.0 31.0 34.0 11.0 34.0 4 30.267919516429274 34.0 32.0 34.0 11.0 34.0 5 30.218621237790465 34.0 32.0 34.0 11.0 34.0 6 32.80911296563552 37.0 32.0 38.0 11.0 38.0 7 31.972286712262868 37.0 30.0 38.0 11.0 38.0 8 31.44463617159231 37.0 27.0 38.0 11.0 38.0 9 31.967277404781328 37.0 31.0 38.0 12.0 38.0 10-11 32.17357930183431 37.0 31.0 38.0 11.0 38.0 12-13 32.50785907008847 37.0 32.0 38.0 11.0 38.0 14-15 32.140516826671686 37.0 31.0 38.0 11.0 38.0 16-17 31.94892911673046 37.0 31.0 38.0 11.0 38.0 18-19 31.993641630587103 37.0 29.5 38.0 11.0 38.0 20-21 32.08602622827382 37.0 31.0 38.0 11.0 38.0 22-23 31.96872581623476 37.0 31.0 38.0 11.0 38.0 24-25 32.34973646232039 37.0 31.0 38.0 11.0 38.0 26-27 32.37099726004476 37.0 31.0 38.0 11.0 38.0 28-29 32.434105122252205 37.0 31.5 38.0 11.0 38.0 30-31 32.28211081131957 37.0 31.0 38.0 11.0 38.0 32-33 32.11704942377277 37.0 31.0 38.0 11.0 38.0 34-35 32.01806592625128 37.0 31.0 38.0 11.0 38.0 36-37 31.955956788186818 37.0 29.0 38.0 11.0 38.0 38-39 32.376043169981806 37.0 31.0 38.0 11.0 38.0 40-41 31.958351634561083 37.0 30.5 38.0 11.0 38.0 42-43 32.3009819915919 37.0 31.0 38.0 11.0 38.0 44-45 31.411301792474532 37.0 27.0 38.0 11.0 38.0 46-47 32.071223149484425 37.0 31.0 38.0 11.0 38.0 48-49 31.901633515299828 37.0 29.5 38.0 11.0 38.0 50-51 31.872246972454036 37.0 29.0 38.0 11.0 38.0 52-53 31.867436364016648 37.0 31.0 38.0 11.0 38.0 54-55 31.62755432850181 37.0 27.0 38.0 11.0 38.0 56-57 31.620809018845037 37.0 29.0 38.0 11.0 38.0 58-59 31.528298927025162 37.0 29.0 38.0 11.0 38.0 60-61 31.594763757294345 37.0 27.0 38.0 11.0 38.0 62-63 31.574114743469075 37.0 27.5 38.0 11.0 38.0 64-65 31.631293007885212 37.0 27.5 38.0 11.0 38.0 66-67 31.61756708707201 37.0 27.5 38.0 11.0 38.0 68-69 31.16259333626153 37.0 26.5 38.0 11.0 38.0 70-71 31.004209282382718 36.5 26.5 38.0 11.0 38.0 72-73 31.096552048691724 37.0 27.0 38.0 11.0 38.0 74-75 30.798289096651395 36.0 26.0 38.0 11.0 38.0 76 30.10847922026312 35.0 25.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.3529507016814506 1101 2 -0.01472142908548335 1101 3 -0.20836128672582532 1101 4 -1.5752856017018182 1101 5 -0.32041392250025424 1101 6 -1.852579686656476 1101 7 -0.17758069075241423 1101 8 -0.43216739391048264 1101 9 -0.7328410491555744 1101 10-11 -0.6150883943016652 1101 12-13 -0.3227177073932239 1101 14-15 0.4211222535885142 1101 16-17 0.1501268754036751 1101 18-19 0.2652100183580366 1101 20-21 0.08581747958603358 1101 22-23 0.22298678586096798 1101 24-25 -0.8762120843611676 1101 26-27 -0.07557833059460961 1101 28-29 0.2195493329284588 1101 30-31 -1.1878356864823516 1101 32-33 -0.15698083179615807 1101 34-35 0.3290592287914009 1101 36-37 0.7186595048460553 1101 38-39 -0.34512971903574297 1101 40-41 0.5122576662421032 1101 42-43 -0.05179742682521038 1101 44-45 0.46095170269913766 1101 46-47 0.7673733949286827 1101 48-49 -0.31692357467704113 1101 50-51 -0.45735699161947707 1101 52-53 0.42291473484637976 1101 54-55 -0.5146322570401303 1101 56-57 -0.4037647087132221 1101 58-59 0.2088168570472675 1101 60-61 0.12018154056511676 1101 62-63 -1.13920028323097 1101 64-65 -0.14759528469752325 1101 66-67 0.07291923776483245 1101 68-69 0.8678089578849786 1101 70-71 0.3489491161494662 1101 72-73 -0.01707668389512662 1101 74-75 -1.192583745269829 1101 76 -1.0015254868822616 1102 1 -0.008101513318912623 1102 2 -0.523888049688729 1102 3 -0.14921585529583226 1102 4 -0.027923193137706193 1102 5 0.16688178623152794 1102 6 0.9756037563077982 1102 7 1.1774945768760574 1102 8 1.1900020089233365 1102 9 0.3310765525985211 1102 10-11 0.050795194223013596 1102 12-13 -3.5918109950316435 1102 14-15 -0.41436684780629207 1102 16-17 -2.11719757690226 1102 18-19 -0.41412408884075447 1102 20-21 0.32042769092500833 1102 22-23 -0.26372852377734546 1102 24-25 0.27505097461843775 1102 26-27 -4.446213821538539 1102 28-29 -0.23971217832022518 1102 30-31 -0.018451278028791762 1102 32-33 -5.761507944725238 1102 34-35 -0.013191982676548974 1102 36-37 0.33863628888231645 1102 38-39 0.4679172840781405 1102 40-41 0.5571050120678329 1102 42-43 0.4437252087394299 1102 44-45 -4.996430421008782 1102 46-47 -0.028565653953819492 1102 48-49 0.35597086638941633 1102 50-51 -5.1592990254116025 1102 52-53 -0.8448887770287712 1102 54-55 0.3697669838347082 1102 56-57 -0.5427472598896799 1102 58-59 1.1182838111496736 1102 60-61 0.7174122077476959 1102 62-63 -3.886376595577808 1102 64-65 -0.4374355367565599 1102 66-67 0.15825632987324667 1102 68-69 -6.271625014808624 1102 70-71 -0.43011229560223185 1102 72-73 0.4773035788506199 1102 74-75 -5.6932470585195105 1102 76 -2.4711144169211394 1103 1 0.2039472442994672 1103 2 -0.6821158977899948 1103 3 -0.024696399130807123 1103 4 0.0579183446025624 1103 5 0.39627700423433865 1103 6 -1.0769044480991425 1103 7 0.5628204090373288 1103 8 1.443119683559999 1103 9 0.002384569476156173 1103 10-11 -0.3591204175913276 1103 12-13 -0.7784026499777283 1103 14-15 -0.5232979307880044 1103 16-17 -0.5457489130485307 1103 18-19 -0.5392295740728237 1103 20-21 -0.49255871320063704 1103 22-23 -0.4732690769887853 1103 24-25 0.060048630502166134 1103 26-27 -0.7663028979567308 1103 28-29 0.32179743255647253 1103 30-31 0.10888111765803643 1103 32-33 -4.565633589357212 1103 34-35 -1.2147625405762241 1103 36-37 0.1169532134017679 1103 38-39 0.28814700747242483 1103 40-41 0.45937880484795457 1103 42-43 0.4238939851107446 1103 44-45 -4.021864082518398 1103 46-47 -0.3252838911783833 1103 48-49 0.032389056731663146 1103 50-51 0.002702849881416114 1103 52-53 0.051735695545069404 1103 54-55 0.05884340202504745 1103 56-57 0.5760994349063786 1103 58-59 0.3346457427014755 1103 60-61 0.6534178336267367 1103 62-63 0.5391273894198463 1103 64-65 0.5394983591646749 1103 66-67 -0.434617556671526 1103 68-69 -2.7569039646445397 1103 70-71 0.43777331152388044 1103 72-73 0.780327488836555 1103 74-75 -1.3844566225138877 1103 76 -0.1075419837284528 1104 1 -1.6367934964412747 1104 2 -0.06387690407930435 1104 3 0.040991930708468516 1104 4 -0.21580632346172735 1104 5 -0.22908987207593157 1104 6 -0.025192358651203506 1104 7 1.162750527835371 1104 8 2.0019596556075214 1104 9 0.14892544997930557 1104 10-11 0.10911857611936426 1104 12-13 -0.5311629574550167 1104 14-15 0.28980479457887043 1104 16-17 -0.5870487034049248 1104 18-19 0.6887578473108285 1104 20-21 1.611144990503071 1104 22-23 1.1198476246184867 1104 24-25 0.9146258492303332 1104 26-27 0.39788298604047156 1104 28-29 -0.19008237177616394 1104 30-31 -0.2759546615173676 1104 32-33 -0.9248229674145136 1104 34-35 0.30260293811001304 1104 36-37 0.2779245621089679 1104 38-39 -0.39469715744721157 1104 40-41 0.33803009765019354 1104 42-43 0.5962210291358971 1104 44-45 -3.5184748442234977 1104 46-47 0.39428284536948155 1104 48-49 0.9704044305960942 1104 50-51 1.5959593138925996 1104 52-53 0.585697959696013 1104 54-55 0.7503178953863312 1104 56-57 0.4595375900426468 1104 58-59 1.2303131081801624 1104 60-61 0.3662060935848075 1104 62-63 0.6301825955693943 1104 64-65 -0.1791599217577513 1104 66-67 0.3852287046841667 1104 68-69 -3.954667766181924 1104 70-71 -0.5392357730323738 1104 72-73 1.135778222589174 1104 74-75 0.053803335557393694 1104 76 0.5727515173197624 1105 1 -0.3520959875444767 1105 2 0.21883309390632633 1105 3 -0.22575083434894339 1105 4 0.48774568083559444 1105 5 1.0396538330204201 1105 6 0.997301430377199 1105 7 1.457627315588006 1105 8 1.15129155650056 1105 9 0.6762872978272831 1105 10-11 1.2462769726815068 1105 12-13 -0.1653803750337488 1105 14-15 1.0516526425611445 1105 16-17 1.167695135982708 1105 18-19 0.8621346014906415 1105 20-21 0.8506467698625002 1105 22-23 0.9435030331996899 1105 24-25 0.7653208065928538 1105 26-27 0.555332794675401 1105 28-29 -0.3542668879918338 1105 30-31 0.7816898694703411 1105 32-33 1.0849265793524268 1105 34-35 0.7054364840711997 1105 36-37 0.6922332977565908 1105 38-39 0.9315567873590709 1105 40-41 1.0054510336604032 1105 42-43 1.0482118973290184 1105 44-45 1.6874742593822276 1105 46-47 0.6885687514998793 1105 48-49 0.4022717508136431 1105 50-51 0.2647365918083935 1105 52-53 -0.7578728453801986 1105 54-55 -0.4846784116035252 1105 56-57 0.37700888528202725 1105 58-59 0.6350938776691848 1105 60-61 0.501974575421908 1105 62-63 1.1000342037074446 1105 64-65 0.7091701662030303 1105 66-67 0.92420674806025 1105 68-69 1.4192394385451301 1105 70-71 1.7398953624003788 1105 72-73 0.8987339736131474 1105 74-75 -0.044974613814794395 1105 76 0.11804125075157756 1106 1 -0.30747622736496893 1106 2 0.19137807811361895 1106 3 -0.4451337039054586 1106 4 -0.47563810341171475 1106 5 -0.014298631642500226 1106 6 -0.3879263133378785 1106 7 0.8058815424599075 1106 8 0.0342397192583519 1106 9 0.5819026417653141 1106 10-11 -0.1360280481134204 1106 12-13 -0.05238747817942624 1106 14-15 -0.01573434310393651 1106 16-17 0.3001680882901603 1106 18-19 -0.5952536704583657 1106 20-21 -0.6792164552800628 1106 22-23 -0.15504060755549887 1106 24-25 0.23773716240756926 1106 26-27 -0.15329531205757974 1106 28-29 -0.9807014518701251 1106 30-31 -0.8229350358448642 1106 32-33 0.4356604757922753 1106 34-35 -0.08573532058514743 1106 36-37 0.4388853865387681 1106 38-39 -0.16046968150570962 1106 40-41 -0.1842401152769817 1106 42-43 0.26485784053242867 1106 44-45 0.26718098664715484 1106 46-47 0.04410477459582296 1106 48-49 0.28022484432648653 1106 50-51 0.2830018235538816 1106 52-53 0.30474774373784186 1106 54-55 -0.03870233648454402 1106 56-57 0.9502627548528331 1106 58-59 -0.726487679877728 1106 60-61 0.04029869038711098 1106 62-63 0.8208319855822737 1106 64-65 0.9006764046800768 1106 66-67 0.8111209529224013 1106 68-69 0.7759831393269039 1106 70-71 0.24214635927042494 1106 72-73 0.09404904171473305 1106 74-75 -0.8347243601310197 1106 76 0.4192317779850647 1107 1 -0.8130524892470312 1107 2 -0.30921421913292235 1107 3 -0.3392714057921431 1107 4 -0.2562569818029772 1107 5 -0.6981274721845274 1107 6 -0.25289698740103717 1107 7 -0.8644111390575269 1107 8 0.38359397079687696 1107 9 -1.2592461259674863 1107 10-11 -0.9768662209486436 1107 12-13 -0.37419268461668054 1107 14-15 -1.1706200730741791 1107 16-17 -0.32196793782870614 1107 18-19 0.19794068971614465 1107 20-21 -0.9665528512235504 1107 22-23 -1.6955046218862009 1107 24-25 -0.8936950412271187 1107 26-27 -0.7386583469308832 1107 28-29 -1.4144002759433931 1107 30-31 -0.6797146723766048 1107 32-33 -0.2688843221043484 1107 34-35 -0.43153578864443176 1107 36-37 -1.1609311457775497 1107 38-39 -0.5279467841609389 1107 40-41 -0.12136992949789871 1107 42-43 -0.22670553527294857 1107 44-45 -0.12451529488183866 1107 46-47 -0.4795871152657796 1107 48-49 -0.6989725206730846 1107 50-51 -0.08557319594859081 1107 52-53 0.17583641496953106 1107 54-55 -0.3853479115907241 1107 56-57 -0.414108398826631 1107 58-59 -0.6562282247911924 1107 60-61 -1.2718419585395289 1107 62-63 -0.9429136444197468 1107 64-65 -0.4539868526658566 1107 66-67 0.49986152672841655 1107 68-69 0.5774552146786718 1107 70-71 -0.9141984444047075 1107 72-73 -0.7168347813472984 1107 74-75 -1.393889076550657 1107 76 -2.0120184025933625 1108 1 -0.6896236851205195 1108 2 0.3361230959206942 1108 3 0.09759570429337217 1108 4 0.4445710350288401 1108 5 0.47657050528255596 1108 6 0.254052924367663 1108 7 0.551429773118393 1108 8 1.42460116504148 1108 9 0.06968016696043478 1108 10-11 0.7430808402703093 1108 12-13 0.6386483632997013 1108 14-15 0.8388613983524564 1108 16-17 -0.21723738265020742 1108 18-19 0.07932388504667287 1108 20-21 1.1715223489936335 1108 22-23 0.33305517178829547 1108 24-25 -0.36839301869419927 1108 26-27 -0.31532456112933716 1108 28-29 0.626898760299305 1108 30-31 0.06366797999206142 1108 32-33 0.9223468439062401 1108 34-35 0.681848221128881 1108 36-37 -0.1447169473249943 1108 38-39 1.260019823684864 1108 40-41 0.6814263240652885 1108 42-43 1.0716927272491077 1108 44-45 0.3815251557765009 1108 46-47 1.0452262415958984 1108 48-49 0.510027072105526 1108 50-51 0.5997328987982513 1108 52-53 0.27060362007337346 1108 54-55 1.0314499708580342 1108 56-57 1.2670847598539687 1108 58-59 -0.3979887786122873 1108 60-61 1.1001683577357504 1108 62-63 0.46980523707882327 1108 64-65 1.0397080027705456 1108 66-67 0.35692681789171843 1108 68-69 1.1774077055161847 1108 70-71 1.7022736609298939 1108 72-73 1.0659669018344573 1108 74-75 1.5858788072555114 1108 76 1.0784118946782542 1109 1 -1.779650639298417 1109 2 -2.3322990479731445 1109 3 -1.1420753165097217 1109 4 -0.926981940297722 1109 5 -1.0101943022369682 1109 6 -0.9275352987299925 1109 7 -1.4913513451344222 1109 8 -0.8222852818449624 1109 9 0.33608420317579046 1109 10-11 -0.14538415385506553 1109 12-13 -0.31177668335175923 1109 14-15 -0.8309291884192653 1109 16-17 -1.2645523318519487 1109 18-19 -0.9134053982486456 1109 20-21 -0.1328110534529756 1109 22-23 -0.37109162481133495 1109 24-25 -0.3783315078779701 1109 26-27 -0.282074163727998 1109 28-29 -0.5529685422095021 1109 30-31 -0.7197310109540922 1109 32-33 0.0985096747860581 1109 34-35 -0.5596750354887163 1109 36-37 0.11111275756131889 1109 38-39 -0.05737598805057331 1109 40-41 -0.7076745122053545 1109 42-43 -0.5237250625083014 1109 44-45 -0.3483801590344697 1109 46-47 -1.0201963860908805 1109 48-49 -0.48315835881575353 1109 50-51 0.11871831589687787 1109 52-53 -0.1112679674585948 1109 54-55 -0.453041009859362 1109 56-57 0.09746695627390167 1109 58-59 -1.0613656533099167 1109 60-61 -0.6408434606345601 1109 62-63 -1.4695824192899636 1109 64-65 -0.5873636676973497 1109 66-67 -0.16339248544645102 1109 68-69 0.6919767842362141 1109 70-71 -0.9587671850166153 1109 72-73 -0.9798068212977569 1109 74-75 0.932454244396979 1109 76 -0.2839766601631304 1110 1 -0.2185425458709318 1110 2 -0.36589283061636024 1110 3 -0.7974326334639983 1110 4 -0.5920886966592285 1110 5 -0.856236726226161 1110 6 -0.8290520329463469 1110 7 -0.06009185684071383 1110 8 1.1721296821517484 1110 9 -1.7209360895859476 1110 10-11 -1.2335817610640802 1110 12-13 -0.8623344905635619 1110 14-15 -0.5766777364491809 1110 16-17 -0.3370881610417733 1110 18-19 -0.3351104359232657 1110 20-21 -0.720033716721268 1110 22-23 -0.7632071138849952 1110 24-25 -0.47703065410563283 1110 26-27 -1.868154749808582 1110 28-29 -0.38013187317511665 1110 30-31 -1.0571800004943377 1110 32-33 0.3790080104174649 1110 34-35 -0.4731557246348075 1110 36-37 -1.1559731349998614 1110 38-39 -0.5853708069199186 1110 40-41 -0.5137024513131436 1110 42-43 0.17345038561771275 1110 44-45 0.3741716870915184 1110 46-47 -0.4045262514093366 1110 48-49 0.14609135218301006 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42-43 0.7593865627085847 2116 44-45 1.8738099315766732 2116 46-47 0.8283673308938901 2116 48-49 1.0932827439411348 2116 50-51 0.5540373827948954 2116 52-53 1.0841243595126784 2116 54-55 0.22567501096192188 2116 56-57 0.41865454061938223 2116 58-59 -0.035724627668891173 2116 60-61 -0.7073558955680461 2116 62-63 1.5022702894048479 2116 64-65 1.4498447379321036 2116 66-67 0.48640508540374583 2116 68-69 0.9792333872822887 2116 70-71 -1.527303911448076 2116 72-73 1.281519571607582 2116 74-75 -0.6636514043402464 2116 76 -0.27645786317064847 2117 1 0.5908487799814885 2117 2 0.02113613473033027 2117 3 0.4808139893064123 2117 4 -0.29971489839653387 2117 5 -0.44220538058950964 2117 6 -1.1713037254252505 2117 7 -1.3790043425435172 2117 8 -2.2087321682918493 2117 9 0.31620570768754064 2117 10-11 -0.5127789541766958 2117 12-13 0.3248042154242654 2117 14-15 -1.1823765215739144 2117 16-17 -0.3653966095254937 2117 18-19 -0.2152458342354251 2117 20-21 -0.45267614770831344 2117 22-23 -0.1608932865642103 2117 24-25 -0.2695358320091259 2117 26-27 0.7700566323052414 2117 28-29 0.24858153373082104 2117 30-31 -0.6464486023058313 2117 32-33 0.4588018216635703 2117 34-35 -0.08553941501163109 2117 36-37 -1.1218453412504417 2117 38-39 -0.18290086967301278 2117 40-41 -1.6672697949713715 2117 42-43 -1.3500897547266533 2117 44-45 0.40744937728286956 2117 46-47 -0.2757355623617599 2117 48-49 -0.5511249450750277 2117 50-51 0.4158013141993919 2117 52-53 0.6574267524556419 2117 54-55 -0.5368657217328554 2117 56-57 -0.6745780718686909 2117 58-59 -0.46255389596157315 2117 60-61 0.6784855843042372 2117 62-63 -0.4587497560182783 2117 64-65 0.3161921261023437 2117 66-67 -1.4970385142144345 2117 68-69 -0.9453411823500453 2117 70-71 0.4664092645500766 2117 72-73 0.7117638031996982 2117 74-75 -0.3646734246819072 2117 76 -1.089926807576692 2118 1 0.3872065035587262 2118 2 0.5912174355433386 2118 3 -0.30217788061228745 2118 4 0.09332575200997084 2118 5 -0.10727855132121533 2118 6 0.3063548111601193 2118 7 0.5825241127410337 2118 8 -3.4753988349585185 2118 9 0.08371790280949298 2118 10-11 -0.5937870842579969 2118 12-13 0.48389364631857035 2118 14-15 -0.9297423752324505 2118 16-17 -0.036860024159643956 2118 18-19 -1.1345629074061563 2118 20-21 -2.5468550094969302 2118 22-23 -0.3684542621739695 2118 24-25 -0.10054396209042693 2118 26-27 0.3338452501914162 2118 28-29 -0.12857293781389956 2118 30-31 -0.08882258604566573 2118 32-33 -0.20059655232017448 2118 34-35 0.2968670890534071 2118 36-37 -0.5185282680797094 2118 38-39 -0.28511225178683475 2118 40-41 -0.7791397136705562 2118 42-43 -0.1454393482225882 2118 44-45 -0.2234937121480236 2118 46-47 -0.9523209282154212 2118 48-49 -0.6504257580831556 2118 50-51 -0.6238897427111816 2118 52-53 -1.5262643044549336 2118 54-55 0.34669525387690214 2118 56-57 1.2558772126841582 2118 58-59 0.862275372331105 2118 60-61 -1.2343599441510413 2118 62-63 -0.014798536506081206 2118 64-65 -0.545612751946436 2118 66-67 -2.2604694085233774 2118 68-69 1.3105775168369398 2118 70-71 -1.2327452069946396 2118 72-73 -1.452561400052332 2118 74-75 -0.45423440029166073 2118 76 -0.26309753928401136 2119 1 0.5755958840896973 2119 2 0.7529873470477604 2119 3 0.5062114999186846 2119 4 0.7122284068772267 2119 5 0.753995784961969 2119 6 1.2708503863813547 2119 7 -1.029051108497903 2119 8 1.6644241738910424 2119 9 0.7259833895351555 2119 10-11 0.6675757476004094 2119 12-13 0.9847078056106113 2119 14-15 -0.7500432601882068 2119 16-17 0.29535236522088937 2119 18-19 0.24779107489472452 2119 20-21 -0.2392974873730367 2119 22-23 1.2304837909233726 2119 24-25 0.6497569228653219 2119 26-27 0.6960045422268166 2119 28-29 1.0492146728055687 2119 30-31 0.7781862635118557 2119 32-33 1.1855627397152233 2119 34-35 1.2661945226817295 2119 36-37 -0.04727163091156683 2119 38-39 0.2231355358237792 2119 40-41 -0.026396350838702176 2119 42-43 -0.0660765163641841 2119 44-45 -0.6691928271922727 2119 46-47 0.06599765585537654 2119 48-49 -0.4687089439238612 2119 50-51 1.1220571599436866 2119 52-53 -0.21153864073811945 2119 54-55 -1.1295702328487636 2119 56-57 1.0330807525071641 2119 58-59 1.1147178502072208 2119 60-61 0.6743126222206364 2119 62-63 -0.31467464270076917 2119 64-65 0.49431645159339155 2119 66-67 0.917955370237685 2119 68-69 -0.2701127486497832 2119 70-71 -0.12012573796809178 2119 72-73 -1.1382074177514454 2119 74-75 0.2966151572304625 2119 76 1.341327239477053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 29.0 13 182.0 14 672.0 15 1335.0 16 2004.0 17 2396.0 18 2498.0 19 2486.0 20 2060.0 21 1652.0 22 1319.0 23 1156.0 24 1120.0 25 1148.0 26 1257.0 27 1440.0 28 1700.0 29 2016.0 30 2402.0 31 3118.0 32 3954.0 33 5134.0 34 7063.0 35 10547.0 36 17324.0 37 19607.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.911670229908054 20.11669176733262 9.510168402649787 33.46146960010953 2 18.832486247934575 26.77731066072661 29.19411850829307 25.196084583045742 3 19.927422559662002 24.34377026207358 27.877475894668592 27.851331283595826 4 11.911484804752044 23.978791491497773 33.33333333333333 30.776390370416852 5 11.646901340695656 34.757691744577606 35.467779381313925 18.127627533412813 6 23.483089665558136 35.85994854740541 18.13913116228483 22.517830624751625 7 23.5583861454477 30.852732634749323 19.852126079772436 25.73675514003054 8 20.987847984773378 37.06678379452427 18.578360628307294 23.367007592395055 9 21.499236577356676 21.34341469536299 19.598000460145155 37.55934826713518 10-11 21.434920834117673 26.567630879923033 26.443182531216664 25.554265754742634 12-13 21.488778732927567 25.021438581079668 25.58250193470122 27.90728075129154 14-15 20.438811152245297 25.83035284767104 23.177197716006777 30.553638284076882 16-17 21.055301081341113 28.30049570182594 24.60417058835833 26.04003262847462 18-19 20.35619418125536 27.378636715400223 25.228503900775973 27.036665202568443 20-21 19.83392856209116 27.484927552903898 25.37034035232662 27.310803532678317 22-23 21.845391227960093 27.01940975926042 25.13386040869256 26.001338604086925 24-25 20.773985066198154 28.925351906465043 24.871368513521993 25.429294513814817 26-27 19.360607391604443 29.540796051117944 25.946957813055572 25.151638744222044 28-29 20.737905502917737 27.98989772228148 27.372884900964213 23.899311873836567 30-31 20.716989814059524 27.377590930957314 27.519817615193155 24.385601639790007 32-33 19.2675325761854 29.436740499048337 26.474556064503986 24.82117086026228 34-35 20.31959172575349 28.41396331388174 26.32291732028194 24.943527640082827 36-37 20.44560875112422 27.61602978394093 26.608416473196545 25.3299449917383 38-39 20.06703478279057 27.04293990922591 27.904666290184267 24.98535901779925 40-41 19.592771537930602 26.469327142289433 27.893685553533704 26.04421576624626 42-43 18.695488485913284 25.94957227416285 28.664428687958836 26.69051055196503 44-45 18.411035117441592 26.106439940599447 29.33477651586455 26.147748426094413 46-47 18.66463784484742 26.681098491978833 28.63357804689298 26.02068561628077 48-49 17.990106879170064 27.266214887787328 28.15983769425446 26.583840538788145 50-51 17.86775009934952 27.244776306707664 27.72845161155383 27.159021982388992 52-53 17.13047206709753 27.303863127732114 28.309907761812138 27.255757043358226 54-55 16.98092489176131 27.7896300014641 27.379682499843135 27.84976260693146 56-57 17.563426826462532 27.346217397669992 27.304908912175023 27.785446863692453 58-59 17.65231850410993 26.837443266193972 27.1893497312334 28.320888498462697 60-61 17.265901152454454 26.231411181527264 27.498901926334945 29.003785739683334 62-63 17.765786116165735 26.70672021083014 27.176277425697016 28.351216247307104 64-65 18.819413942398192 26.939407249377755 25.40628725607078 28.83489155215327 66-67 18.134948024513186 28.06519420217105 24.638158582752922 29.16169919056284 68-69 17.99638158582753 27.552236932923385 25.602894731337976 28.848486749911107 70-71 18.32214343979419 28.439062140511595 25.313212440651732 27.92558197904248 72-73 18.545941310577064 28.35644516952166 24.967057790048315 28.13055572985296 74-75 18.4753508606806 28.823387923281253 24.50116082073163 28.20010039530652 76 18.260442157662464 27.764531174834246 24.884440819058376 29.090585848444917 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 1.5 8 1.0 9 1.0 10 1.0 11 2.5 12 4.0 13 4.0 14 5.0 15 5.5 16 10.5 17 16.0 18 15.5 19 18.5 20 33.0 21 44.0 22 57.5 23 87.0 24 117.5 25 132.0 26 165.0 27 265.5 28 386.5 29 440.0 30 544.5 31 747.5 32 1062.5 33 1279.0 34 1448.5 35 1874.5 36 2364.5 37 2598.0 38 3029.5 39 3871.5 40 4653.5 41 5417.0 42 5809.0 43 6055.5 44 6629.0 45 7044.0 46 7132.0 47 7255.0 48 7130.0 49 6591.0 50 6300.0 51 5825.0 52 4923.5 53 4163.0 54 3829.0 55 3474.0 56 2712.5 57 2027.0 58 1748.0 59 1538.0 60 1178.0 61 878.5 62 729.0 63 626.5 64 427.5 65 273.5 66 174.0 67 132.0 68 132.0 69 97.0 70 58.0 71 54.0 72 42.5 73 29.0 74 26.0 75 25.0 76 19.0 77 10.5 78 8.5 79 9.0 80 6.5 81 5.5 82 5.5 83 4.0 84 3.5 85 5.0 86 5.5 87 4.0 88 3.5 89 2.5 90 5.0 91 8.5 92 9.0 93 5.5 94 1.5 95 3.0 96 5.0 97 4.5 98 5.0 99 7.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7017213611930309 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.228922214553137E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 95622.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.39685428039573 #Duplication Level Percentage of deduplicated Percentage of total 1 80.46464010080328 53.42598983497522 2 9.41250590644196 12.499215661667817 3 4.191211214364467 8.348497207755537 4 2.0239407780752874 5.375332036560624 5 1.189163647818554 3.9478362719876183 6 0.6945975744211688 2.7671456359415196 7 0.5402425578831311 2.510928447428416 8 0.3764372342101118 1.999539854845119 9 0.2567333438336746 1.5341657777498903 >10 0.8505276421483697 7.5913492710882435 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0010457844429106274 0.0 5 0.0 0.0 0.0 0.0010457844429106274 0.0 6 0.0 0.0 0.0 0.0010457844429106274 0.0 7 0.0 0.0 0.0 0.0010457844429106274 0.0 8 0.0 0.0 0.0 0.0020915688858212547 0.0 9 0.0 0.0 0.0 0.005228922214553136 0.0 10 0.0 0.0 0.0 0.005228922214553136 0.0 11 0.0 0.0 0.0 0.008366275543285019 0.0 12 0.0 0.0 0.0 0.008366275543285019 0.0 13 0.0 0.0 0.0 0.009412059986195645 0.0 14 0.0 0.0 0.0 0.013595197757838155 0.0 15 0.0 0.0 0.0 0.01568676664365941 0.0 16 0.0 0.0 0.0 0.01882411997239129 0.0 17 0.0 0.0 0.0 0.02614461107276568 0.0 18 0.0 0.0 0.0 0.03032774884440819 0.0 19 0.0 0.0 0.0 0.033465102173140075 0.0 20 0.0 0.0 0.0 0.03555667105896133 0.0 21 0.0 0.0 0.0 0.03764823994478258 0.0 22 0.0 0.0 0.0 0.04183137771642509 0.0 23 0.0 0.0 0.0 0.04496873104515697 0.0 24 0.0 0.0 0.0 0.05333500658844199 0.0 25 0.0 0.0 0.0 0.06065549768881638 0.0 26 0.0 0.0 0.0 0.06797598878919077 0.0 27 0.0 0.0 0.0 0.09830373763359897 0.0 28 0.0 0.0 0.0 0.14327246867875593 0.0 29 0.0 0.0 0.0 0.18196649306644913 0.0 30 0.0 0.0 0.0 0.21229424191085733 0.0 31 0.0 0.0 0.0 0.26981238627094184 0.0 32 0.0 0.0 0.0 0.3189642550877413 0.0 33 0.0 0.0 0.0 0.381711321662379 0.0 34 0.0 0.0 0.0 0.4873355503963523 0.0 35 0.0 0.0 0.0 0.5908682102445044 0.0 36 0.0 0.0 0.0 0.751919014452741 0.0 37 0.0 0.0 0.0 1.060425425111376 0.0 38 0.0 0.0 0.0 1.4912886155905545 0.0 39 0.0 0.0 0.0 1.9482964171424986 0.0 40 0.0 0.0 0.0 2.561126100688126 0.0 41 0.0 0.0 0.0 3.3036330551546715 0.0 42 0.0 0.0 0.0 4.146535316140637 0.0 43 0.0 0.0 0.0 5.1170232791617 0.0 44 0.0 0.0 0.0 5.983978582334609 0.0 45 0.0 0.0 0.0 6.826880843320574 0.0 46 0.0 0.0 0.0 7.691744577607664 0.0 47 0.0 0.0 0.0 8.445755160946225 0.0 48 0.0 0.0 0.0 9.140156031038883 0.0 49 0.0 0.0 0.0 9.791679738972203 0.0 50 0.0 0.0 0.0 10.450523938005897 0.0 51 0.0 0.0 0.0 11.213946581330656 0.0 52 0.0 0.0 0.0 12.047436782330426 0.0 53 0.0 0.0 0.0 12.92171257660371 0.0 54 0.0 0.0 0.0 13.866055928552006 0.0 55 0.0 0.0 0.0 14.594967685260714 0.0 56 0.0 0.0 0.0 15.216163644349626 0.0 57 0.0 0.0 0.0 15.858275292296751 0.0 58 0.0 0.0 0.0 16.523394197987912 0.0 59 0.0 0.0 0.0 17.12367446821861 0.0 60 0.0 0.0 0.0 17.69153542071908 0.0 61 0.0 0.0 0.0 18.20606136663111 0.0 62 0.0 0.0 0.0 18.888958607851748 0.0 63 0.0 0.0 0.0 19.518520842483948 0.0 64 0.0 0.0 0.0 20.144945723787412 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCCG 15 0.002219771 69.99111 17 GACGTCG 15 0.002219771 69.99111 23 CAATTGG 15 0.002219771 69.99111 23 GGTTCTG 15 0.002219771 69.99111 23 CGAAGTG 15 0.002219771 69.99111 31 ACTGGGG 25 2.3814206E-4 55.99289 3 TGTTTAT 20 0.006936138 52.49333 39 CTTTTTA 20 0.006936138 52.49333 44 ACCTAGT 20 0.006936138 52.49333 46 CCGAGCT 20 0.006936138 52.49333 68 GCTCGGA 20 0.006936138 52.49333 11 CAATCCC 20 0.006936138 52.49333 67 ATTGGGC 30 5.8559125E-4 46.66074 52 TACAAGG 30 5.8559125E-4 46.66074 2 GAGACCT 35 0.0012507845 39.99492 44 ACAAGGA 35 0.0012507845 39.99492 3 CAGATAC 50 1.6130069E-4 35.309498 1 GTGGGGC 40 0.002409894 34.995552 9 GCTGAGG 40 0.002409894 34.995552 24 GGGCCTT 40 0.002409894 34.995552 12 >>END_MODULE