FastQCFastQC Report
Wed 25 May 2016
SRR1780452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95622
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT37863.959339900859635No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT34113.56717073476815TruSeq Adapter, Index 9 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC11121.1629123005166175RNA PCR Primer, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC5480.5730898747150237TruSeq Adapter, Index 9 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG3630.3796197527765577No Hit
TATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT3230.33778837506013265RNA PCR Primer, Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT2360.24680512852690803No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC2100.21961473301123172No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTCTT1650.1725544330802535No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC1640.1715086486373429No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG1420.14850139089330908No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGT1200.1254941331492753No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGT1200.1254941331492753No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC1010.10562422873397335No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG970.10144109096233084No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC970.10144109096233084TruSeq Adapter, Index 9 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTCTT960.10039530651942022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA1250.058.81
TTTACAC2700.057.0370372
TTACACA2750.056.03
TGTTTAC200.00693265552.553
CCGGATC200.00693265552.543
GAAAGTC200.00693265552.542
CAGATAT200.00693265552.51
CTTTACA3000.051.3333321
CTTATAA352.0730084E-550.01
TATGATC305.852248E-446.66666430
GTGTCTC305.852248E-446.66666460
CTTAATA551.2957753E-744.5454522
TTAATAC551.2957753E-744.5454523
ATAACAC350.001250003740.04
GATTCAA350.001250003740.020
TAATACA806.1494575E-835.04
ATGATCT400.00240839335.031
TTACTCG400.00240839335.04
TCTTAAT751.472974E-632.6666681
ATATCGT552.9731164E-431.8181837
Produced by FastQC (version 0.11.5)