##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780452_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 95622 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.01060425425111 34.0 34.0 34.0 32.0 34.0 2 33.34550626424881 34.0 34.0 34.0 33.0 34.0 3 33.51131538767229 34.0 34.0 34.0 34.0 34.0 4 33.51205789462676 34.0 34.0 34.0 34.0 34.0 5 33.52700215431595 34.0 34.0 34.0 34.0 34.0 6 37.28552006860346 38.0 38.0 38.0 36.0 38.0 7 36.8762941582481 38.0 37.0 38.0 35.0 38.0 8 36.92628265461923 38.0 37.0 38.0 35.0 38.0 9 36.69103344418649 38.0 37.0 38.0 34.0 38.0 10-11 36.42396624207818 38.0 37.0 38.0 34.0 38.0 12-13 36.26354813745791 38.0 37.0 38.0 34.0 38.0 14-15 36.46273347137688 38.0 37.0 38.0 34.0 38.0 16-17 36.586821024450444 38.0 37.0 38.0 34.0 38.0 18-19 36.621007717889185 38.0 37.0 38.0 34.0 38.0 20-21 36.35747526719793 38.0 37.0 38.0 34.0 38.0 22-23 36.42032168329464 38.0 37.0 38.0 34.0 38.0 24-25 35.63727489489867 38.0 36.0 38.0 31.5 38.0 26-27 35.87925895714375 38.0 37.0 38.0 32.0 38.0 28-29 35.73254062872561 38.0 36.5 38.0 31.0 38.0 30-31 35.41332015644936 38.0 36.0 38.0 29.0 38.0 32-33 35.89463198845454 38.0 37.0 38.0 32.5 38.0 34-35 35.81754198824538 38.0 37.0 38.0 32.0 38.0 36-37 35.861961682458016 38.0 37.0 38.0 32.0 38.0 38-39 35.249529397000686 38.0 36.0 38.0 29.0 38.0 40-41 35.17417017004455 37.0 35.5 38.0 29.0 38.0 42-43 35.774994248185564 38.0 36.5 38.0 32.5 38.0 44-45 36.01155591809416 38.0 37.0 38.0 33.0 38.0 46-47 36.2477358766811 38.0 37.0 38.0 34.0 38.0 48-49 36.02714856413796 38.0 37.0 38.0 33.0 38.0 50-51 36.152459685009724 38.0 37.0 38.0 34.0 38.0 52-53 36.04583150321056 38.0 37.0 38.0 33.5 38.0 54-55 36.23764928572922 38.0 37.0 38.0 34.0 38.0 56-57 36.2617493882161 38.0 37.0 38.0 34.0 38.0 58-59 36.27083725502499 38.0 37.0 38.0 34.0 38.0 60-61 36.00603940515781 38.0 37.0 38.0 32.5 38.0 62-63 36.32014599150823 38.0 37.0 38.0 34.0 38.0 64-65 36.3043598753425 38.0 37.0 38.0 34.0 38.0 66-67 35.47351550898329 38.0 36.0 38.0 31.0 38.0 68-69 34.28464160967141 37.5 34.5 38.0 24.0 38.0 70-71 33.65963899521031 37.0 34.0 38.0 22.0 38.0 72-73 32.91061157474222 37.0 33.0 38.0 16.0 38.0 74-75 33.31611449248081 37.5 34.0 38.0 19.0 38.0 76 32.8455376377821 37.0 33.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2566320802642821 1101 2 0.42095398708125487 1101 3 0.2579593505779343 1101 4 0.2870986803010851 1101 5 0.2751687875968827 1101 6 -1.3709995700540105 1101 7 0.27971577416575144 1101 8 0.48935474986385685 1101 9 0.2758668845624541 1101 10-11 0.18874321046708076 1101 12-13 0.6676866870811864 1101 14-15 0.650907764448867 1101 16-17 0.03633190421020771 1101 18-19 -0.676914778680235 1101 20-21 0.413205926029633 1101 22-23 0.7227693818346381 1101 24-25 -1.1335837380475908 1101 26-27 -0.3579674148820473 1101 28-29 0.884052848523659 1101 30-31 1.4133075767205412 1101 32-33 0.573119272966764 1101 34-35 0.6798599977637423 1101 36-37 0.1200303190167773 1101 38-39 0.32999666417107676 1101 40-41 1.3187760163171163 1101 42-43 0.7592491492662461 1101 44-45 0.345613750215918 1101 46-47 0.7263995214062149 1101 48-49 -1.0661982657478006 1101 50-51 -1.20166705081472 1101 52-53 -1.2638844073069606 1101 54-55 -0.649345063668072 1101 56-57 -1.1266863348718203 1101 58-59 -0.9440084479259525 1101 60-61 -0.9634906284505362 1101 62-63 -0.3692477490887711 1101 64-65 -1.4248013158378683 1101 66-67 -1.944549619519897 1101 68-69 -0.5761648405494668 1101 70-71 0.20791341538853914 1101 72-73 -0.44568255785517863 1101 74-75 0.36537019921064484 1101 76 0.2750884950398529 1102 1 -0.45567422305413174 1102 2 0.29404005197540073 1102 3 0.33328225515295884 1102 4 0.39995405958728725 1102 5 0.3046678018396918 1102 6 0.3486584551150429 1102 7 0.2191147386600676 1102 8 0.48075010298603615 1102 9 0.6904008148777123 1102 10-11 0.5932629532489386 1102 12-13 0.6826296605670876 1102 14-15 0.08289973098840164 1102 16-17 0.32197117235459416 1102 18-19 -0.19943057843332923 1102 20-21 0.6043414814623489 1102 22-23 0.5494235192510359 1102 24-25 -4.679776135740738 1102 26-27 -1.0170796806495588 1102 28-29 0.2785860418241981 1102 30-31 1.7205229508476378 1102 32-33 0.4009342085701064 1102 34-35 0.8333801643290712 1102 36-37 -2.059681508226973 1102 38-39 -0.032946235613344754 1102 40-41 1.460347331704142 1102 42-43 1.1031310058063255 1102 44-45 1.1466934767834616 1102 46-47 1.2471280604587847 1102 48-49 -3.4485430780854216 1102 50-51 -0.523834611111738 1102 52-53 0.03732429683691407 1102 54-55 0.6496894470462777 1102 56-57 1.061538012409521 1102 58-59 0.5501875611393245 1102 60-61 -5.924438060875495 1102 62-63 -2.514347522456738 1102 64-65 -0.029715361495931347 1102 66-67 1.140428177911602 1102 68-69 -1.0610652514351742 1102 70-71 -1.7104161407443428 1102 72-73 -5.2942720959312055 1102 74-75 -2.0502508278193687 1102 76 -0.38446597956077966 1103 1 0.5925208074193833 1103 2 0.4389064373481162 1103 3 -0.1031921939797229 1103 4 0.2795133657288673 1103 5 0.16111412157715677 1103 6 0.28405461076435756 1103 7 0.787187875087632 1103 8 0.7094420861084032 1103 9 0.4360641997815975 1103 10-11 0.24532577556492186 1103 12-13 -0.9387017974732288 1103 14-15 -0.4425339305212077 1103 16-17 -0.052993665901389875 1103 18-19 0.44494354252307033 1103 20-21 -0.3378291702019638 1103 22-23 0.3225833879805293 1103 24-25 -2.847403890077352 1103 26-27 -0.7045386586148652 1103 28-29 -0.11632722587388145 1103 30-31 1.1418778500506406 1103 32-33 0.9767663698453077 1103 34-35 0.07419122855786497 1103 36-37 -1.1386547853015117 1103 38-39 -0.25922377897949644 1103 40-41 -0.14504413571265218 1103 42-43 -0.277787887770792 1103 44-45 0.9970450942236795 1103 46-47 0.8284454538014927 1103 48-49 -2.477422590509235 1103 50-51 -0.4560662097990331 1103 52-53 0.22933086036245953 1103 54-55 -0.07609583190355806 1103 56-57 0.069367360745197 1103 58-59 -0.28354427195959175 1103 60-61 -4.1107249807067205 1103 62-63 -1.2076433499297892 1103 64-65 0.4597126741346358 1103 66-67 -0.47152681505604477 1103 68-69 0.5095864128873799 1103 70-71 0.06490031495186344 1103 72-73 -4.606906882616627 1103 74-75 -2.8983286524794707 1103 76 -4.101249852040855 1104 1 0.6031427501167954 1104 2 0.44226073504203356 1104 3 0.36109851182042263 1104 4 -0.14739089702445085 1104 5 -0.17026253810139025 1104 6 0.027171312371628176 1104 7 0.46790066334759217 1104 8 0.7018949162970785 1104 9 0.9819062682651776 1104 10-11 -0.1577839379221473 1104 12-13 0.7070703898083934 1104 14-15 0.6979622260126845 1104 16-17 0.41136692110071493 1104 18-19 0.8834131442002118 1104 20-21 -0.12039381031377872 1104 22-23 0.4685302782670391 1104 24-25 0.5125891218024492 1104 26-27 1.0988505796800325 1104 28-29 1.3558251151463807 1104 30-31 0.7864969974999738 1104 32-33 0.519778249649633 1104 34-35 0.33544210207288216 1104 36-37 1.4222466822037276 1104 38-39 1.5598887297556487 1104 40-41 1.8574017063558372 1104 42-43 0.7441548096436037 1104 44-45 0.7375063101565971 1104 46-47 0.8324636229139983 1104 48-49 0.44787440459907657 1104 50-51 0.6734585980276577 1104 52-53 0.6417347737097714 1104 54-55 0.3928070332257221 1104 56-57 0.5506391986208072 1104 58-59 -0.4560460189896389 1104 60-61 0.6870737614316411 1104 62-63 0.5166054271491802 1104 64-65 0.7680634777684645 1104 66-67 -0.023308785705935975 1104 68-69 1.985547279414284 1104 70-71 1.9318814470273367 1104 72-73 1.302352376642638 1104 74-75 0.24740859420116834 1104 76 0.024466430279204587 1105 1 0.887209090008021 1105 2 0.3194916488941786 1105 3 0.1477633879367133 1105 4 -0.13157138450178962 1105 5 -0.5115101040719807 1105 6 -0.08049000502580839 1105 7 0.7570969300500678 1105 8 -0.5204511523257622 1105 9 0.7512744278032173 1105 10-11 0.2596067825505628 1105 12-13 -0.4923857305165953 1105 14-15 0.10903454186577477 1105 16-17 0.06934718028287534 1105 18-19 0.5359478920683429 1105 20-21 0.4904099229837442 1105 22-23 0.08206012450352773 1105 24-25 0.486845483851738 1105 26-27 0.2314900747448263 1105 28-29 0.8841538601943952 1105 30-31 1.2033975548087739 1105 32-33 0.8266741067637184 1105 34-35 -0.620859516137017 1105 36-37 0.44175249030148933 1105 38-39 0.06980614061981782 1105 40-41 0.5068208965794341 1105 42-43 0.5048007506897321 1105 44-45 0.6679031408824372 1105 46-47 1.0019593591659302 1105 48-49 -0.4612238294706188 1105 50-51 0.7118591889092798 1105 52-53 0.2797526344497143 1105 54-55 0.64749715610224 1105 56-57 0.5608923376794266 1105 58-59 0.3462369327326442 1105 60-61 0.8244470240382427 1105 62-63 -0.3070634105598131 1105 64-65 0.8576894765598411 1105 66-67 0.5889318642638415 1105 68-69 0.27796653678351646 1105 70-71 1.2793131263397655 1105 72-73 1.23936305279922 1105 74-75 -0.2619045059129803 1105 76 0.2678156547470749 1106 1 -0.9423297057443847 1106 2 0.09887966358640199 1106 3 0.07172971588195054 1106 4 0.11989712298009891 1106 5 0.11443853032324824 1106 6 0.08441159425387212 1106 7 -0.9066541597182578 1106 8 0.282132849160611 1106 9 -0.8662561929002379 1106 10-11 0.4213855702994209 1106 12-13 1.0185048067213245 1106 14-15 -0.687625407540402 1106 16-17 0.16280285350959645 1106 18-19 0.6570586696984364 1106 20-21 0.20034575170789282 1106 22-23 0.42474760038570025 1106 24-25 -0.633057616092529 1106 26-27 0.7744662499081159 1106 28-29 0.5467472944295579 1106 30-31 0.6593923525049021 1106 32-33 -0.20406807906142888 1106 34-35 0.7941599925025287 1106 36-37 1.2154951477640807 1106 38-39 0.2976927761298285 1106 40-41 0.8795431021275988 1106 42-43 0.6949921211537955 1106 44-45 0.8562151076105593 1106 46-47 0.5488158241718608 1106 48-49 0.9657724117219075 1106 50-51 -0.2815235948949777 1106 52-53 0.5663009629952143 1106 54-55 -0.2138914588558123 1106 56-57 0.03906838707272442 1106 58-59 0.5221005294683394 1106 60-61 0.943692689976011 1106 62-63 0.7573722680978747 1106 64-65 -1.5640044164499187 1106 66-67 -0.2985683143866993 1106 68-69 1.071210687764669 1106 70-71 0.03779188121692556 1106 72-73 -0.1305793502649948 1106 74-75 -0.8485071442242287 1106 76 -0.4188312888993977 1107 1 0.7309966933772856 1107 2 -0.19858357064548926 1107 3 0.12609104609488497 1107 4 0.19968389597134006 1107 5 -0.03347687229441476 1107 6 0.05138741119068868 1107 7 0.6130072196120153 1107 8 -0.20710467648152786 1107 9 -0.2798963650998303 1107 10-11 0.5238096551279483 1107 12-13 0.1166672406296243 1107 14-15 0.1388676149091097 1107 16-17 0.5528016978076451 1107 18-19 0.42404026514497417 1107 20-21 0.6398953168750339 1107 22-23 0.07660683588886741 1107 24-25 0.24886576086855428 1107 26-27 -0.16474382781284191 1107 28-29 0.6696706424980832 1107 30-31 0.4961277834372595 1107 32-33 0.8489353013667511 1107 34-35 -0.16239283752261002 1107 36-37 1.1724163577840159 1107 38-39 0.07421613575538544 1107 40-41 0.402664364154127 1107 42-43 0.7124865593381884 1107 44-45 0.6432481317936194 1107 46-47 0.9690796131406856 1107 48-49 0.6778798342176486 1107 50-51 1.0032196948106105 1107 52-53 0.6176069061924636 1107 54-55 0.7073041148057442 1107 56-57 0.9418160684456964 1107 58-59 0.8741562343020632 1107 60-61 1.0706898873987925 1107 62-63 0.8204808174030163 1107 64-65 0.8052495779449274 1107 66-67 0.07111906823732284 1107 68-69 0.47862451573842435 1107 70-71 -0.30740184332152154 1107 72-73 0.4440789953476738 1107 74-75 -0.98427323015067 1107 76 0.3544922209674084 1108 1 -0.6421402687511275 1108 2 0.27622299919298143 1108 3 -0.22003356365127757 1108 4 0.18845815957932643 1108 5 0.018416707181629022 1108 6 -0.34641359328875154 1108 7 -0.08304273287882324 1108 8 -0.5245201780425433 1108 9 -0.37658429777255975 1108 10-11 0.3044802130212503 1108 12-13 0.861787370940462 1108 14-15 0.3828678863900379 1108 16-17 -0.3527840222955163 1108 18-19 -0.43168119542242067 1108 20-21 0.5003609066673533 1108 22-23 0.12324725939911474 1108 24-25 1.0635325180288646 1108 26-27 -0.47662111843317234 1108 28-29 -0.9894579037215365 1108 30-31 0.22989322391507017 1108 32-33 0.7386461741779371 1108 34-35 0.6467628567898629 1108 36-37 1.1222466822037234 1108 38-39 1.09196420145377 1108 40-41 0.8724960459784796 1108 42-43 -0.1747131148846961 1108 44-45 0.7563742346848912 1108 46-47 0.4720862644234316 1108 48-49 0.8648555366745541 1108 50-51 0.40364727727293825 1108 52-53 -0.24128409421475538 1108 54-55 -0.7109665516799382 1108 56-57 -0.01539853722825768 1108 58-59 0.2873502074254475 1108 60-61 0.7795265916203249 1108 62-63 1.0656620309227662 1108 64-65 0.5378747985231769 1108 66-67 0.8370685727846308 1108 68-69 0.8251699209237202 1108 70-71 -0.5077411944821009 1108 72-73 -0.32217592524416006 1108 74-75 -0.5148555567422335 1108 76 0.8772966189584466 1109 1 -1.3623835474849706 1109 2 0.21790521634689242 1109 3 0.3062502154626898 1109 4 -0.0293592431437375 1109 5 -0.12427456381153235 1109 6 -0.20589247918272946 1109 7 -0.568245580345824 1109 8 0.04350802017433608 1109 9 0.4492917130091172 1109 10-11 0.11014059037974278 1109 12-13 0.4093649505935133 1109 14-15 0.5423053043180275 1109 16-17 0.5476168347091175 1109 18-19 0.6423183898979161 1109 20-21 0.8065949242221251 1109 22-23 0.2906949060920425 1109 24-25 -0.35047674315021027 1109 26-27 0.17810277402656993 1109 28-29 -1.2458958054424585 1109 30-31 0.2657077238805101 1109 32-33 0.22900487208380582 1109 34-35 -0.4918852875186559 1109 36-37 0.8123220156752922 1109 38-39 0.589718019962298 1109 40-41 0.37477678410827764 1109 42-43 0.988186878203976 1109 44-45 0.5808057779843594 1109 46-47 0.7612700366260796 1109 48-49 0.3847670716804288 1109 50-51 0.007324103591273001 1109 52-53 -0.7277102466786474 1109 54-55 -0.7957132515209722 1109 56-57 -0.09151990014206746 1109 58-59 -0.2354571738724971 1109 60-61 0.7293849094031728 1109 62-63 0.25845351616708 1109 64-65 0.8654717298936987 1109 66-67 0.2926978491686185 1109 68-69 -1.1844713811704182 1109 70-71 -0.816967816916268 1109 72-73 -0.9251201508298905 1109 74-75 -0.9194723236231468 1109 76 -0.3136633644543636 1110 1 -1.0198718133382556 1110 2 -0.3312667776920222 1110 3 -0.016303023440357833 1110 4 -0.12954169693836093 1110 5 0.35297442294315573 1110 6 0.1285272202559824 1110 7 0.5390678919866616 1110 8 0.679784291431595 1110 9 0.8007311480987411 1110 10-11 -0.7111521852856555 1110 12-13 0.5528627708974199 1110 14-15 0.9066070355399063 1110 16-17 0.49673172488864026 1110 18-19 -0.373413026974184 1110 20-21 0.5468420028722463 1110 22-23 -0.5513850671666347 1110 24-25 -1.0909118467031789 1110 26-27 1.0150712554817432 1110 28-29 0.18355307267562182 1110 30-31 -0.31267080506835754 1110 32-33 0.43553978204077026 1110 34-35 0.49025234671023554 1110 36-37 1.191742341863673 1110 38-39 0.45328854322864487 1110 40-41 -1.3742935372053964 1110 42-43 -0.2407508507337539 1110 44-45 0.4963268855206806 1110 46-47 0.4759387646027946 1110 48-49 0.947616136430625 1110 50-51 -0.8813372983781136 1110 52-53 0.20006033508433063 1110 54-55 -0.6823203073295545 1110 56-57 0.7524542499157363 1110 58-59 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-0.42163732809531496 2116 42-43 -0.896621296077889 2116 44-45 0.1995642125322732 2116 46-47 -0.6549628532910177 2116 48-49 0.24499456348686266 2116 50-51 -0.5945652351401662 2116 52-53 0.2654457201615372 2116 54-55 -0.1793646878039894 2116 56-57 0.21742554038232242 2116 58-59 -1.9373079546706435 2116 60-61 -1.3300907027040196 2116 62-63 0.37923624221296137 2116 64-65 -1.5015034002393293 2116 66-67 0.0283832164329354 2116 68-69 -0.6301046952247802 2116 70-71 1.0213780477453795 2116 72-73 0.44243334830255066 2116 74-75 0.42860941919543905 2116 76 -0.5739294141940974 2117 1 0.9035415842938193 2117 2 0.5879579278630231 2117 3 0.3633841384502716 2117 4 0.22495148677674592 2117 5 0.13383318210109252 2117 6 0.337080807692999 2117 7 0.7435564387732683 2117 8 0.08098680155555371 2117 9 -0.6447849420431524 2117 10-11 -0.43457485677473073 2117 12-13 -1.4902374794967201 2117 14-15 0.013202293507696083 2117 16-17 -0.8994706306710327 2117 18-19 -0.74028680978045 2117 20-21 -0.1037731629752301 2117 22-23 0.040650235315666805 2117 24-25 -1.970529454666334 2117 26-27 -2.282542271984333 2117 28-29 -1.4924338202731562 2117 30-31 0.020152466130134883 2117 32-33 -1.4719689508412372 2117 34-35 0.9250494038062769 2117 36-37 -0.729847197225638 2117 38-39 -0.36121113218636225 2117 40-41 0.04280590945447216 2117 42-43 0.49095646633941925 2117 44-45 -0.19577333396060226 2117 46-47 -0.9755744197305702 2117 48-49 -0.9185698353150258 2117 50-51 -0.4664094798984024 2117 52-53 0.9272080058005798 2117 54-55 0.16229621868361477 2117 56-57 0.2721225549638078 2117 58-59 -0.8994882647328524 2117 60-61 -0.2521883953408661 2117 62-63 -0.22372284405805942 2117 64-65 -0.9393839834986011 2117 66-67 -0.29496854947338846 2117 68-69 0.5149612999695776 2117 70-71 -0.07940862813756411 2117 72-73 0.6104824579434478 2117 74-75 0.9906054035277521 2117 76 1.0447302744270814 2118 1 -0.318442155543579 2118 2 -0.00287134042966386 2118 3 -0.6905996013871274 2118 4 -0.11056070834520426 2118 5 0.06007708454011862 2118 6 -0.09713057442082373 2118 7 0.7574100973098581 2118 8 0.5494420861083995 2118 9 -1.8335654298480293 2118 10-11 -0.9558594096202597 2118 12-13 -1.1986277233991558 2118 14-15 0.3191697731824945 2118 16-17 -2.3681047770124977 2118 18-19 -0.49794534636581744 2118 20-21 -0.42990324427604065 2118 22-23 0.7913982027953423 2118 24-25 0.8709664802930135 2118 26-27 -2.2281682882444898 2118 28-29 -0.5364012999479542 2118 30-31 -0.3809369647641745 2118 32-33 -0.2808632597843257 2118 34-35 -0.058105067738438265 2118 36-37 -3.2324325630792927 2118 38-39 -0.3881867419424623 2118 40-41 -0.07067376534227776 2118 42-43 0.45524914926624405 2118 44-45 -1.8613993502207649 2118 46-47 -5.175091614688654E-4 2118 48-49 0.029836668750022 2118 50-51 -0.820881024613854 2118 52-53 -1.4540765470449486 2118 54-55 -0.8559476837554101 2118 56-57 0.17595995333778802 2118 58-59 -1.0549516793670008 2118 60-61 -1.4800583140400576 2118 62-63 -1.39082853511497 2118 64-65 -0.1888799184579497 2118 66-67 0.03291762938840037 2118 68-69 -1.122339513038547 2118 70-71 -0.23109968504813594 2118 72-73 0.8803523766426338 2118 74-75 0.5015972734464498 2118 76 0.45978718499618054 2119 1 0.43663749047411926 2119 2 -0.365420012996033 2119 3 0.02479862870136884 2119 4 -0.5520828322390088 2119 5 -0.29008220749528135 2119 6 -0.46927216734118105 2119 7 -0.48351025667244585 2119 8 0.11386686486946473 2119 9 -0.20241498737015462 2119 10-11 -0.5049036574078656 2119 12-13 -0.5035657764965009 2119 14-15 -0.5175912887644074 2119 16-17 -0.38490123718948865 2119 18-19 -0.44792764725077205 2119 20-21 -1.6229651911787002 2119 22-23 -0.34180533702766525 2119 24-25 0.9282762148062886 2119 26-27 0.5737963135254205 2119 28-29 -0.3488083795939687 2119 30-31 1.1367975485101596 2119 32-33 0.1922694835785066 2119 34-35 0.22568254288103162 2119 36-37 -0.5960785807784106 2119 38-39 -1.5173194853052934 2119 40-41 -0.7742312874661721 2119 42-43 -0.23632607197269095 2119 44-45 0.15835286216861988 2119 46-47 -0.37965025252429996 2119 48-49 -0.746039437444665 2119 50-51 0.6667295948551768 2119 52-53 -0.04798805146972285 2119 54-55 -0.16600078110054284 2119 56-57 -1.9415798696710667 2119 58-59 -0.6413941572431057 2119 60-61 -0.07591672111970382 2119 62-63 -0.2839789775928452 2119 64-65 -0.2563312458915803 2119 66-67 0.31849285062733657 2119 68-69 0.8960498674924295 2119 70-71 0.07153748309345787 2119 72-73 1.2660691908019217 2119 74-75 1.0094556805261021 2119 76 0.797734087651051 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 2.0 21 2.0 22 11.0 23 27.0 24 54.0 25 115.0 26 233.0 27 409.0 28 716.0 29 1185.0 30 1902.0 31 2981.0 32 4417.0 33 6654.0 34 10113.0 35 13111.0 36 19414.0 37 34275.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.21319361653176 21.035954069147266 10.65236033548765 31.09849197883332 2 19.048963627617074 18.353516973081508 40.293028800903556 22.30449059839786 3 16.711635397711824 25.178306247516264 34.49310828052122 23.616950074250695 4 11.869653427035619 21.889314174562337 35.31823220597771 30.922800192424337 5 11.459705925414653 37.873083600008364 36.94756436803246 13.71964610654452 6 23.745581560728702 38.95756206730669 17.40708205224739 19.889774319717223 7 24.19003994896572 33.66798435506473 20.050825123925456 22.09115057204409 8 21.24092781995775 35.817071385246074 22.05873125431386 20.883269540482317 9 20.49319194327665 19.270669929514128 18.674572797055074 41.561565330154146 10-11 20.944447930392588 25.161050804208234 29.849302461776578 24.045198803622597 12-13 23.278638806969106 20.442471397795487 27.58727071176089 28.691619083474514 14-15 22.947125138566438 23.56727531321244 21.319361653176045 32.16623789504507 16-17 24.17278450565769 27.468574177490535 22.004350463282506 26.35429085356926 18-19 22.38344732383761 26.733910606345816 22.968563719646106 27.914078350170463 20-21 21.524858296207984 25.045491623266614 22.76934178327163 30.66030829725377 22-23 23.175106147120957 29.326410240321266 22.89222145531363 24.60626215724415 24-25 23.78113823178766 30.707368597184747 22.914705820836208 22.596787350191377 26-27 24.583254899500115 28.614231034699127 25.853360105415074 20.949153960385686 28-29 23.32413043023572 26.23297985819163 28.17761602978394 22.26527368178871 30-31 26.280563050344064 24.288866578820773 27.30072577440338 22.129844596431784 32-33 22.29089540064002 26.6335152998264 30.113885925832967 20.96170337370061 34-35 20.533977536550164 25.772834703310956 31.21509694421786 22.47809081592102 36-37 22.61247411683504 27.09522913137144 28.11330028654494 22.17899646524858 38-39 21.252954341051222 24.43109326305662 28.426512727196666 25.88943966869549 40-41 19.931605697433643 24.792934680303695 30.85220974252787 24.42324987973479 42-43 24.004936102570536 23.57877894208446 31.573801008136204 20.8424839472088 44-45 19.756436803246118 25.383279998326746 29.72746857417749 25.13281462424965 46-47 23.938005898224258 21.761728472527242 28.429127188303948 25.871138440944552 48-49 21.76643450252034 21.80983455690113 29.368764510259144 27.054966430319382 50-51 17.626696785258623 22.202526615214072 32.80887243521365 27.36190416431365 52-53 18.808433205747633 22.24174353182322 36.17577544916442 22.77404781326473 54-55 17.356361506766223 22.611951224613584 33.21045366129133 26.82123360732886 56-57 21.68172596264458 22.226579657401015 30.183430591286527 25.908263788667877 58-59 17.61571604860806 27.376545146514403 32.37225743029847 22.635481374579072 60-61 19.424923134843446 32.832402585179146 25.80002509882663 21.94264918115078 62-63 15.965991089916546 37.8176570245341 23.993955365919977 22.222396519629374 64-65 16.414632615925207 38.6438267344335 22.41272928823911 22.528811361402187 66-67 16.09514546861601 38.221329819497605 21.948923887808245 23.734600824078143 68-69 16.146388906318627 36.50310597979545 22.30971952061241 25.04078559327351 70-71 16.882621154127712 34.57833971261843 22.735876681098492 25.80316245215536 72-73 17.442115831084898 32.41461170023634 23.10765305055322 27.035619418125535 74-75 18.130764886741545 31.889627909895214 23.128045847189977 26.851561356173264 76 17.933111627031437 32.082575139612224 23.116019326096506 26.868293907259837 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 1.5 14 1.5 15 4.0 16 5.0 17 4.0 18 5.0 19 9.5 20 19.5 21 26.0 22 34.0 23 51.5 24 71.0 25 81.0 26 130.0 27 231.5 28 342.0 29 400.0 30 484.0 31 636.0 32 925.5 33 1147.0 34 1295.5 35 1680.0 36 2059.0 37 2202.0 38 2612.5 39 3717.5 40 5626.0 41 7914.0 42 8988.0 43 9033.5 44 8774.5 45 8082.5 46 7695.0 47 7278.0 48 6395.0 49 5722.5 50 5516.0 51 5021.5 52 4089.0 53 3397.0 54 3143.0 55 2850.5 56 2187.5 57 1622.5 58 1428.0 59 1164.0 60 779.5 61 558.5 62 458.0 63 353.5 64 200.5 65 122.5 66 69.0 67 45.0 68 33.5 69 17.0 70 8.0 71 4.0 72 2.0 73 0.5 74 1.0 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 95622.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.56365689903997 #Duplication Level Percentage of deduplicated Percentage of total 1 62.44967793880838 25.95636987304177 2 16.99627616747182 14.128547823722574 3 7.918176328502416 9.873250925519232 4 4.000603864734299 6.65118905691159 5 2.292169887278583 4.7635481374579065 6 1.5096618357487923 3.7648239944782578 7 0.9334742351046699 2.7159021982388993 8 0.7472826086956522 2.4847838363556507 9 0.6416062801932367 2.4000752964798897 >10 2.4532004830917877 15.228713057664553 >50 0.022644927536231884 0.7372780322519922 >100 0.025161030595813202 2.0330049570182593 >500 0.0025161030595813203 0.5730898747150237 >1k 0.007548309178743961 8.689422936144402 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT 3786 3.959339900859635 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT 3411 3.56717073476815 TruSeq Adapter, Index 9 (95% over 23bp) CTTTACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 1112 1.1629123005166175 RNA PCR Primer, Index 9 (95% over 21bp) CTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 548 0.5730898747150237 TruSeq Adapter, Index 9 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 363 0.3796197527765577 No Hit TATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCT 323 0.33778837506013265 RNA PCR Primer, Index 9 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT 236 0.24680512852690803 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 210 0.21961473301123172 No Hit ATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGTCTT 165 0.1725544330802535 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 164 0.1715086486373429 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 142 0.14850139089330908 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGACCTCGTATGCCGT 120 0.1254941331492753 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGT 120 0.1254941331492753 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC 101 0.10562422873397335 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG 97 0.10144109096233084 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTC 97 0.10144109096233084 TruSeq Adapter, Index 9 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACTGGATCTGAACTCGTATGCCGTCTT 96 0.10039530651942022 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0010457844429106274 0.0 5 0.0 0.0 0.0 0.0010457844429106274 0.0 6 0.0 0.0 0.0 0.0010457844429106274 0.0 7 0.0 0.0 0.0 0.0010457844429106274 0.0 8 0.0 0.0 0.0 0.0020915688858212547 0.0 9 0.0 0.0 0.0 0.007320491100374391 0.0 10 0.0 0.0 0.0 0.007320491100374391 0.0 11 0.0 0.0 0.0 0.010457844429106272 0.0 12 0.0 0.0 0.0 0.010457844429106272 0.0 13 0.0 0.0 0.0 0.0115036288720169 0.0 14 0.0 0.0 0.0 0.014640982200748782 0.0 15 0.0 0.0 0.0 0.01568676664365941 0.0 16 0.0 0.0 0.0 0.01986990441530192 0.0 17 0.0 0.0 0.0 0.02719039551567631 0.0 18 0.0 0.0 0.0 0.03137353328731882 0.0 19 0.0 0.0 0.0 0.03555667105896133 0.0 20 0.0 0.0 0.0 0.03660245550187195 0.0 21 0.0 0.0 0.0 0.04392294660224635 0.0 22 0.0 0.0 0.0 0.04810608437388885 0.0 23 0.0 0.0 0.0 0.05333500658844199 0.0 24 0.0 0.0 0.0 0.06065549768881638 0.0 25 0.0 0.0 0.0 0.06797598878919077 0.0 26 0.0 0.0 0.0 0.07634226433247579 0.0 27 0.0 0.0 0.0 0.11189893539143712 0.0 28 0.0 0.0 0.0 0.15895923532241535 0.0 29 0.0 0.0 0.0 0.20392796636757232 0.0 30 0.0 0.0 0.0 0.24262199075526553 0.0 31 0.0 0.0 0.0 0.30536905732990316 0.0 32 0.0 0.0 0.0 0.35765827947543455 0.0 33 0.0 0.0 0.0 0.42772583715044654 0.0 34 0.0 0.0 0.0 0.5249837903411348 0.0 35 0.0 0.0 0.0 0.6379285101754826 0.0 36 0.0 0.0 0.0 0.8125745121415574 0.0 37 0.0 0.0 0.0 1.1378134738867625 0.0 38 0.0 0.0 0.0 1.5780887243521364 0.0 39 0.0 0.0 0.0 2.059149568091025 0.0 40 0.0 0.0 0.0 2.7148564137959883 0.0 41 0.0 0.0 0.0 3.528476710380456 0.0 42 0.0 0.0 0.0 4.432034469055238 0.0 43 0.0 0.0 0.0 5.455857438664743 0.0 44 0.0 0.0 0.0 6.382422455083558 0.0 45 0.0 0.0 0.0 7.291209135972894 0.0 46 0.0 0.0 0.0 8.232415134592458 0.0 47 0.0 0.0 0.0 9.044989646734015 0.0 48 0.0 0.0 0.0 9.789588170086382 0.0 49 0.0 0.0 0.0 10.493401100165235 0.0 50 0.0 0.0 0.0 11.197214030244085 0.0 51 0.0 0.0 0.0 12.018154817928929 0.0 52 0.0 0.0 0.0 12.902888456631318 0.0 53 0.0 0.0 0.0 13.840957101922152 0.0 54 0.0 0.0 0.0 14.847001736002175 0.0 55 0.0 0.0 0.0 15.645981050385894 0.0 56 0.0 0.0 0.0 16.28495534500429 0.0 57 0.0 0.0 0.0 16.965761017339105 0.0 58 0.0 0.0 0.0 17.6737570851896 0.0 59 0.0 0.0 0.0 18.32005187090837 0.0 60 0.0 0.0 0.0 18.928698416682355 0.0 61 0.0 0.0 0.0 19.48923887808245 0.0 62 0.0 0.0 0.0 20.209784359247873 0.0 63 0.0 0.0 0.0 20.89059003158269 0.0 64 0.0 0.0 0.0 21.559892075045493 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACA 125 0.0 58.8 1 TTTACAC 270 0.0 57.037037 2 TTACACA 275 0.0 56.0 3 TGTTTAC 20 0.006932655 52.5 53 CCGGATC 20 0.006932655 52.5 43 GAAAGTC 20 0.006932655 52.5 42 CAGATAT 20 0.006932655 52.5 1 CTTTACA 300 0.0 51.333332 1 CTTATAA 35 2.0730084E-5 50.0 1 TATGATC 30 5.852248E-4 46.666664 30 GTGTCTC 30 5.852248E-4 46.666664 60 CTTAATA 55 1.2957753E-7 44.545452 2 TTAATAC 55 1.2957753E-7 44.545452 3 ATAACAC 35 0.0012500037 40.0 4 GATTCAA 35 0.0012500037 40.0 20 TAATACA 80 6.1494575E-8 35.0 4 ATGATCT 40 0.002408393 35.0 31 TTACTCG 40 0.002408393 35.0 4 TCTTAAT 75 1.472974E-6 32.666668 1 ATATCGT 55 2.9731164E-4 31.81818 37 >>END_MODULE