FastQCFastQC Report
Wed 25 May 2016
SRR1780451_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780451_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917491
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGTC450.001396744224.44808223
CCGTAAC450.001401637124.43341331
TCGGTGG3200.023.35553242
CGGTGGT2200.020.97907843
TCTATCG654.945943E-420.29852731
TTGCGAT550.004447899820.02702516
CGAGCTC550.00449452419.99097327
ATCCTCG1352.57387E-919.56914734
ATCGGTT1252.2922904E-819.35548825
TTTAGCG1152.029683E-719.12180126
CCGATAA708.119109E-418.85789122
TACCGCG1051.8136197E-618.8332239
TATCGGC1303.529385E-818.6405415
CGGTACT1303.6256097E-818.59683843
GTAGATC6350.018.37296138
GCCGAAA600.007341682418.36312520
TCCGATA600.00734399818.3621221
CAGTAGT600.0074207418.3290581
GCATATA600.0074207418.3290581
ACGGCCT600.0074675718.309086
Produced by FastQC (version 0.11.5)