FastQCFastQC Report
Wed 25 May 2016
SRR1780451_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780451_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917491
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGT437354.76680425203081No Hit
ATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCTT372534.060312308240626No Hit
CTTTACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC107031.1665509525434037No Hit
CTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC58120.6334667043055463No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG48610.5298144613952617No Hit
TATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTCT32780.3572787090009602No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC29570.3222919897851859No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC20770.2263782424023778No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG14770.16098250555046315No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCG12180.13275334580938666No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCC10300.11226268159578677No Hit
CTTAACACATCTCCGAGCCCACGAGACCAAGAATTATCTCGTATGCCGTC10240.11160872422726763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT40550.042.85737644
GCCGTCT44200.039.21870843
CCGCTTG250.00235338535.19729638
TTTACAC14600.034.357272
CTTATAA1750.033.975431
CTTTACA15900.032.1314351
CTATACA8200.031.1518171
TTACACA16400.030.9888023
CTTTATA3650.030.1379572
GACTCGT300.00571743429.3614851
GGTCATA554.9224655E-628.0268731
TTATACA72600.027.8796862
TGCCGTC63350.027.39805442
CTTATAC76100.026.708921
GCTTATA508.285037E-526.4253351
TCGTTGT858.236384E-925.88036534
CATCGTT858.236384E-925.88036532
TATACAC79450.025.6974933
TCTTTAT4250.025.3890481
GGAACCG450.001398138824.44395
Produced by FastQC (version 0.11.5)