FastQCFastQC Report
Wed 25 May 2016
SRR1780450_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780450_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39068
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAT150.002209922569.99103532
GGTACCG150.002209922569.99103526
CGAAATA150.002209922569.99103564
GTCTAGA150.002209922569.99103564
TGTACGA150.002209922569.99103560
GTGGTCA150.002209922569.99103510
TAATGTA150.002209922569.99103557
CTGGCAG150.002209922569.99103550
CACCCGG150.002209922569.99103513
TACCGTG150.002209922569.99103528
TGCAATA150.002209922569.99103533
CATGGTG200.00690559852.4932842
CAGGTGG200.00690559852.493287
CTTTTTA200.00690559852.4932842
TTATGGT200.00690559852.4932822
AATATTC200.00690559852.4932836
ATGTACG200.00690559852.4932859
TGGCAGT200.00690559852.4932851
ACAGGTG200.00690559852.493286
ATTCATG200.00690559852.4932839
Produced by FastQC (version 0.11.5)