##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780450_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 39068 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.721588000409543 32.0 21.0 34.0 12.0 34.0 2 28.096114467083034 33.0 23.0 34.0 11.0 34.0 3 28.065296406265997 34.0 23.0 34.0 11.0 34.0 4 28.752073308078224 34.0 23.0 34.0 11.0 34.0 5 28.62764922698884 34.0 23.0 34.0 11.0 34.0 6 30.581319750179176 37.0 26.0 38.0 11.0 38.0 7 29.9079297634893 35.0 24.0 38.0 11.0 38.0 8 29.024649329374423 34.0 22.0 37.0 11.0 38.0 9 29.786526057131155 34.0 23.0 37.0 12.0 38.0 10-11 30.155331729292513 35.5 25.0 38.0 11.0 38.0 12-13 30.529858195966007 36.0 25.5 38.0 11.0 38.0 14-15 30.04910668577864 36.0 24.0 38.0 11.0 38.0 16-17 29.896692945633255 36.0 23.0 38.0 11.0 38.0 18-19 29.897588819494217 36.0 23.5 38.0 11.0 38.0 20-21 30.07860653220027 36.0 24.0 38.0 11.0 38.0 22-23 29.94801371966827 36.0 23.0 38.0 11.0 38.0 24-25 30.293565066038703 36.0 24.0 38.0 11.0 38.0 26-27 30.435945018941332 36.5 25.0 38.0 11.0 38.0 28-29 30.412626702160335 37.0 24.5 38.0 11.0 38.0 30-31 30.2497184396437 36.0 24.0 38.0 11.0 38.0 32-33 30.158249718439645 36.0 24.0 38.0 11.0 38.0 34-35 30.016816832190027 36.0 24.0 38.0 11.0 38.0 36-37 29.91944814170165 36.0 23.5 38.0 11.0 38.0 38-39 30.372888297327737 36.5 25.0 38.0 11.0 38.0 40-41 29.981084263335724 36.0 23.5 38.0 11.0 38.0 42-43 30.380516023343915 36.0 25.5 38.0 11.0 38.0 44-45 29.34843094092352 34.0 22.0 38.0 11.0 38.0 46-47 30.054827480290776 36.0 24.5 38.0 11.0 38.0 48-49 29.82517661513259 35.5 24.0 38.0 11.0 38.0 50-51 29.830193508753965 35.0 23.5 38.0 11.0 38.0 52-53 29.832151633050067 35.5 23.5 38.0 11.0 38.0 54-55 29.549119483976654 34.5 23.0 38.0 11.0 38.0 56-57 29.513130951162076 35.0 23.0 38.0 11.0 38.0 58-59 29.392328760110576 34.5 23.0 37.5 11.0 38.0 60-61 29.46869560765844 34.5 23.0 38.0 11.0 38.0 62-63 29.460440769939595 34.5 23.0 38.0 11.0 38.0 64-65 29.502009317088152 34.5 23.0 38.0 11.0 38.0 66-67 29.578977679942664 34.5 23.0 38.0 11.0 38.0 68-69 29.0416965291287 34.0 22.0 37.0 11.0 38.0 70-71 28.83977935906624 34.0 21.5 37.0 11.0 38.0 72-73 28.833495443841507 34.0 21.5 37.0 11.0 38.0 74-75 28.403680761748745 33.5 21.0 37.0 11.0 38.0 76 27.50069110269274 32.0 19.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.1145002784266183 1101 2 0.12559002018177878 1101 3 -1.5145776368733657 1101 4 -3.179217785874716 1101 5 -2.237427515979469 1101 6 -2.807059630130663 1101 7 -0.858679218164994 1101 8 -1.229840076217613 1101 9 -0.3711338580638923 1101 10-11 -1.1391234225239053 1101 12-13 -0.8780218159178901 1101 14-15 -0.15704021730545392 1101 16-17 -1.5374476035895874 1101 18-19 -0.5257516114178777 1101 20-21 -1.4530322299923917 1101 22-23 -0.5374528856179595 1101 24-25 -1.8818567036726996 1101 26-27 -0.7500895066394477 1101 28-29 -1.014422542279899 1101 30-31 -1.578632476674418 1101 32-33 -0.042509875000263264 1101 34-35 -1.992930591048811 1101 36-37 -0.9877805443676024 1101 38-39 -1.20719827045777 1101 40-41 -0.9505577610739877 1101 42-43 -1.7274048708327037 1101 44-45 -1.3330930424533882 1101 46-47 -2.140173217592867 1101 48-49 -1.15614161197988 1101 50-51 -1.876947918172764 1101 52-53 -0.9457405888377082 1101 54-55 -1.7449477522741397 1101 56-57 -1.8717135514957235 1101 58-59 -1.6301106397480787 1101 60-61 -1.4393693811623827 1101 62-63 -2.9065629211500443 1101 64-65 -1.0933481903346376 1101 66-67 -2.267216132655239 1101 68-69 -1.6957038607258959 1101 70-71 0.1315726030130442 1101 72-73 -1.7499447121976601 1101 74-75 -2.085995346836853 1101 76 -1.5229942343689125 1102 1 0.3300751932714938 1102 2 -0.2346694137804839 1102 3 0.477167646145503 1102 4 0.19873032733283225 1102 5 0.732305188423048 1102 6 0.23893093590707437 1102 7 2.871470152904184 1102 8 0.9100970307006264 1102 9 1.7153441293574936 1102 10-11 -0.68020046654906 1102 12-13 -3.6687057781820442 1102 14-15 -0.2971738651041953 1102 16-17 -1.55582417591663 1102 18-19 0.4916422565066476 1102 20-21 1.5083474869887397 1102 22-23 0.11201645400468152 1102 24-25 1.1177502145662963 1102 26-27 -3.9717876198469924 1102 28-29 -0.12664738504719608 1102 30-31 0.24850981892306834 1102 32-33 -4.074349497641773 1102 34-35 1.1554577737310616 1102 36-37 1.8137131663242236 1102 38-39 1.2588984276554385 1102 40-41 0.04344774207066493 1102 42-43 0.4420133681610068 1102 44-45 -3.23826206761062 1102 46-47 0.2439659333505304 1102 48-49 0.7798843314163442 1102 50-51 -4.539880308109872 1102 52-53 -1.4791525385232447 1102 54-55 0.3425129395497635 1102 56-57 -0.16642660181019053 1102 58-59 0.9342564986167048 1102 60-61 0.9122422540577446 1102 62-63 -4.153811348823002 1102 64-65 0.28361721847039334 1102 66-67 0.8007870119988532 1102 68-69 -5.505550558839101 1102 70-71 1.2862502759690209 1102 72-73 1.3502911368589423 1102 74-75 -5.01818874306327 1102 76 -0.8461074419160823 1103 1 0.14799972157338104 1103 2 -1.1514933131515548 1103 3 -1.6104109702067007 1103 4 -0.28963445254138165 1103 5 -0.09992751597946636 1103 6 -3.1945596301306622 1103 7 0.4392374485016717 1103 8 -0.1631734095509465 1103 9 0.12886614193610768 1103 10-11 -0.3370400891905696 1103 12-13 -1.9967718159178887 1103 14-15 -1.279956883972119 1103 16-17 -1.0697392702562531 1103 18-19 -2.0497099447512106 1103 20-21 -1.7124072299923903 1103 22-23 -1.7155778856179609 1103 24-25 -1.0610233703393632 1103 26-27 -1.700089506639447 1103 28-29 -0.5373392089465661 1103 30-31 -0.9400908100077494 1103 32-33 -5.8383432083335975 1103 34-35 -1.3991805910488146 1103 36-37 -1.2419472110342653 1103 38-39 -0.30407327045777066 1103 40-41 0.39840057225934444 1103 42-43 0.13509512916729705 1103 44-45 -4.519551375786719 1103 46-47 -0.6474648842595343 1103 48-49 -0.9509332786465485 1103 50-51 -1.1081979181727633 1103 52-53 -0.9290739221710425 1103 54-55 -0.5220310856074697 1103 56-57 0.6293281151709422 1103 58-59 -0.23115230641474227 1103 60-61 0.17521395217095304 1103 62-63 0.04760374551662139 1103 64-65 0.085818476332026 1103 66-67 0.411950534011428 1103 68-69 -1.7050788607258944 1103 70-71 -0.05801073032028725 1103 72-73 0.48338862113567416 1103 74-75 -1.6630786801701873 1103 76 -0.47299423436891175 1104 1 -0.8353336117599532 1104 2 0.9901733535151109 1104 3 1.5812556964599693 1104 4 1.3103655474586162 1104 5 1.0000724840205315 1104 6 0.2471070365360042 1104 7 1.5642374485016717 1104 8 2.3201599237823842 1104 9 2.2871994752694427 1104 10-11 0.13379324414276184 1104 12-13 1.307394850748775 1104 14-15 1.1825431160278832 1104 16-17 0.5260940630770818 1104 18-19 2.0711233885821194 1104 20-21 1.8542594366742726 1104 22-23 1.2052554477153734 1104 24-25 1.451476629660636 1104 26-27 2.204077160027218 1104 28-29 0.8793274577200982 1104 30-31 0.6474091899922492 1104 32-33 -0.49250987500026255 1104 34-35 1.5633194089511875 1104 36-37 2.0038861222990647 1104 38-39 0.4209267295422272 1104 40-41 0.9609005722593444 1104 42-43 1.814261795833957 1104 44-45 0.3137819575466132 1104 46-47 1.2858684490737993 1104 48-49 2.036566721353452 1104 50-51 2.162635415160569 1104 52-53 0.512592744495624 1104 54-55 0.5613022477258625 1104 56-57 1.3584947818376065 1104 58-59 1.5730143602519213 1104 60-61 0.8835472855042887 1104 62-63 2.126770412183287 1104 64-65 0.556651809665361 1104 66-67 1.3619505340114308 1104 68-69 -1.4175788607258966 1104 70-71 0.05865593634637989 1104 72-73 1.520888621135672 1104 74-75 0.38692131982981337 1104 76 1.202005765631089 1105 1 -0.5028549792813202 1105 2 -0.7341856208438635 1105 3 0.003050568254838737 1105 4 0.004809991903062638 1105 5 0.40413231308036046 1105 6 -0.8075938181648503 1105 7 1.1031263373905595 1105 8 -0.736250332627872 1105 9 -0.23651847344850552 1105 10-11 0.3280240133735326 1105 12-13 -1.4755111321572052 1105 14-15 -0.26467910619433965 1105 16-17 0.23699149897451832 1105 18-19 0.35605928601802006 1105 20-21 -0.07950124708640871 1105 22-23 -0.8180351505752235 1105 24-25 0.3836347493187553 1105 26-27 -0.2778672844172263 1105 28-29 -1.0638349354422978 1105 30-31 -0.7352831177000567 1105 32-33 0.43665679166640103 1105 34-35 0.2338322294640065 1105 36-37 0.2777109086238525 1105 38-39 -0.4234108772953782 1105 40-41 -0.20897122261245116 1105 42-43 0.48894128301344963 1105 44-45 1.2832264019910582 1105 46-47 0.26674451744986527 1105 48-49 -0.7940956718089396 1105 50-51 -0.5689885164633637 1105 52-53 -1.6717021272992483 1105 54-55 -0.9567533078296933 1105 56-57 -0.6832787224358974 1105 58-59 -0.7170497423121809 1105 60-61 -0.3755339110769107 1105 62-63 -0.3718406989278229 1105 64-65 -0.11845502794147578 1105 66-67 -6.563035954094687E-4 1105 68-69 0.9759040452570105 1105 70-71 0.1809849961754395 1105 72-73 0.3211022963493484 1105 74-75 -0.4484419280334322 1105 76 -1.2975668839415633 1106 1 -1.512000278426619 1106 2 -0.6831599798182211 1106 3 -2.062077636873365 1106 4 -1.6729677858747145 1106 5 -0.44826084931280263 1106 6 -1.4595596301306628 1106 7 -0.07909588483166274 1106 8 0.7034932571157206 1106 9 0.4155328086027765 1106 10-11 -0.009540089190569034 1106 12-13 -1.0192718159178895 1106 14-15 -0.8424568839721154 1106 16-17 -1.6172392702562526 1106 18-19 -0.8763766114178786 1106 20-21 -1.787407229992393 1106 22-23 -0.7530778856179623 1106 24-25 -0.4810233703393614 1106 26-27 -1.6550895066394489 1106 28-29 -1.6640058756132348 1106 30-31 -1.1659241433410834 1106 32-33 -0.170009875000261 1106 34-35 -0.4100139243821488 1106 36-37 -1.1819472110342666 1106 38-39 -0.4365732704577674 1106 40-41 -0.909099427740653 1106 42-43 -0.6565715374993708 1106 44-45 -0.942884709120051 1106 46-47 -0.3282982175928666 1106 48-49 -0.8792666119798795 1106 50-51 -0.6640312515060955 1106 52-53 -0.299907255504376 1106 54-55 -1.062864418940805 1106 56-57 -0.7656718848290609 1106 58-59 -1.4328189730814103 1106 60-61 -1.012286047829047 1106 62-63 -0.6473962544833789 1106 64-65 -0.7675148570013057 1106 66-67 0.8986172006780997 1106 68-69 0.5149211392741044 1106 70-71 -1.8988440636536197 1106 72-73 -2.0774447121976607 1106 74-75 -2.452245346836854 1106 76 -2.537994234368913 1107 1 -1.0186669450932833 1107 2 0.04017335351511164 1107 3 -0.777077636873365 1107 4 0.5270322141252848 1107 5 -0.20826084931280064 1107 6 0.09710703653600206 1107 7 -0.11909588483166189 1107 8 -0.7131734095509472 1107 9 -1.5044671913972252 1107 10-11 -0.44120675585723745 1107 12-13 -0.15093848258455722 1107 14-15 -1.3466235506387854 1107 16-17 -0.761405936922916 1107 18-19 0.1377900552487894 1107 20-21 -0.8290738966590574 1107 22-23 -0.8739112189512923 1107 24-25 -0.7943567036726975 1107 26-27 -0.6792561733061149 1107 28-29 -1.237339208946569 1107 30-31 -1.590090810007748 1107 32-33 -0.017509875000264685 1107 34-35 -0.1575139243821475 1107 36-37 -1.1586138777009332 1107 38-39 -0.6374066037911028 1107 40-41 0.04840057225934302 1107 42-43 -1.3315715374993715 1107 44-45 -0.5445513757867211 1107 46-47 0.0400351157404657 1107 48-49 -0.48426661197987997 1107 50-51 0.2626354151605703 1107 52-53 0.09592607782895968 1107 54-55 -0.3553644189408054 1107 56-57 -0.40400521816239277 1107 58-59 -0.5103189730814108 1107 60-61 -0.8206193811623805 1107 62-63 -0.8815629211500458 1107 64-65 -0.33501485700130473 1107 66-67 -0.19638279932190628 1107 68-69 1.3657544726074384 1107 70-71 0.11698926967971346 1107 72-73 -0.445778045530993 1107 74-75 -0.4380786801701859 1107 76 -0.44799423436891317 1108 1 -1.0986104479181442 1108 2 -1.3897701493097472 1108 3 -0.9308911961954003 1108 4 -0.883137277400138 1108 5 -0.35995576456704015 1108 6 -1.1365087826730367 1108 7 -0.5512992746621705 1108 8 -0.4854897937317375 1108 9 -0.05616210665146326 1108 10-11 -0.07962483495328243 1108 12-13 -0.2906277481212811 1108 14-15 0.14398379399398564 1108 16-17 -1.850035880425743 1108 18-19 -1.609173221587369 1108 20-21 -0.8335936706703571 1108 22-23 -1.1293490720586377 1108 24-25 -1.5453454042376684 1108 26-27 -1.8433098456224997 1108 28-29 -0.5294296044267917 1108 30-31 -0.9388902450359957 1108 32-33 -1.2935691970341594 1108 34-35 -2.0259461277719772 1108 36-37 -1.0156760245935885 1108 38-39 0.030884356660873635 1108 40-41 -0.947997732825403 1108 42-43 -1.3824189951264927 1108 44-45 -1.518562675221748 1108 46-47 -0.27762025149117164 1108 48-49 -0.0012157645222536928 1108 50-51 -0.384539726082366 1108 52-53 -1.3973648826230196 1108 54-55 0.06349151326258706 1108 56-57 0.277209471103145 1108 58-59 -1.6512088035898849 1108 60-61 0.05974785047603959 1108 62-63 -1.2409555765172762 1108 64-65 -0.4390402807301186 1108 66-67 -1.2845183925422425 1108 68-69 -0.08685852174284392 1108 70-71 0.1381050888887536 1108 72-73 0.23204681322607001 1108 74-75 0.043771602315686664 1108 76 0.1825142402073574 1109 1 0.043528176857932976 1109 2 -0.23950144323285372 1109 3 0.35076789158192057 1109 4 0.9424793685968282 1109 5 1.3149098823945167 1109 6 0.7544241097067363 1109 7 0.0435057411845996 1109 8 -0.23593763719322425 1109 9 1.0536628899035883 1109 10-11 1.1116387725980488 1109 12-13 0.33666314343170356 1109 14-15 0.7624211648083694 1109 16-17 0.622232274459197 1109 18-19 1.145615258500822 1109 20-21 1.4416578106580147 1109 22-23 1.8534261794226907 1109 24-25 1.0851148410427491 1109 26-27 1.0730812250678703 1109 28-29 0.6138803032485534 1109 30-31 1.4992384582849319 1109 32-33 1.6195836209346979 1109 34-35 0.8767340430975281 1109 36-37 1.1011422198600442 1109 38-39 0.4110690059649933 1109 40-41 1.0655753690073126 1109 42-43 1.5424122023380278 1109 44-45 1.8387819575466153 1109 46-47 0.511376579155101 1109 48-49 0.8307740384266253 1109 50-51 1.9927167159735788 1109 52-53 1.8342390859590374 1109 54-55 0.4687209469128568 1109 56-57 0.4022955948457394 1109 58-59 0.5968964740730627 1109 60-61 0.9186082611140414 1109 62-63 0.1701647211263726 1109 64-65 1.3248428665759313 1109 66-67 1.1824789892959835 1109 68-69 1.4317097571602844 1109 70-71 1.4179039038260548 1109 72-73 0.07271788942835755 1109 74-75 1.3278766043826593 1109 76 0.3357456030294621 1110 1 0.5280968089520215 1110 2 0.8680050687254663 1110 3 0.7687961495344027 1110 4 0.6056729908243135 1110 5 1.0281469176774891 1110 6 0.8076248358887561 1110 7 0.8032342122557168 1110 8 1.416600053232223 1110 9 -0.3136904923680994 1110 10-11 -0.2648313513264924 1110 12-13 0.5106310967034702 1110 14-15 0.8895819509793377 1110 16-17 1.0599937394524872 1110 18-19 0.9537690196500854 1110 20-21 0.46186461466780315 1110 22-23 1.1508056095276729 1110 24-25 1.3125203189810257 1110 26-27 -0.06805067168799184 1110 28-29 0.7484213088527838 1110 30-31 0.8216001285035794 1110 32-33 2.192320222087119 1110 34-35 1.4197919008605737 1110 36-37 2.060965410324954 1110 38-39 1.1785723606101932 1110 40-41 1.814055912065168 1110 42-43 1.7606614722093639 1110 44-45 2.1624065530158667 1110 46-47 1.4249056659022798 1110 48-49 1.9744712520977892 1110 50-51 1.671696903833709 1110 52-53 1.1175684726509623 1110 54-55 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2116 56-57 0.1378445986874226 2116 58-59 -1.0027640280264656 2116 60-61 -1.8199783555213536 2116 62-63 0.9737026466155179 2116 64-65 0.3491884397019902 2116 66-67 -0.9689102718493778 2116 68-69 -0.04546347611050905 2116 70-71 -2.582140766950321 2116 72-73 0.3839838592309093 2116 74-75 -1.4441684237599297 2116 76 -1.2084337948084745 2117 1 -0.5785960231074689 2117 2 0.8642868286924177 2117 3 1.531964916318124 2117 4 0.610010937529541 2117 5 -1.3540055301638638 2117 6 -1.5663681407689616 2117 7 -1.9063299273848529 2117 8 -3.2528897216076835 2117 9 0.2902136596666054 2117 10-11 -1.003263493445889 2117 12-13 1.5003026521672211 2117 14-15 -0.9360739052487155 2117 16-17 0.7709522191054496 2117 18-19 0.6394744524119105 2117 20-21 0.14503957851824723 2117 22-23 0.8245816888501238 2117 24-25 1.396955353064893 2117 26-27 2.255229642296719 2117 28-29 1.2140792307697446 2117 30-31 0.6646077715525358 2117 32-33 0.780628422872077 2117 34-35 -0.07426924353108433 2117 36-37 -1.395138700395968 2117 38-39 0.8217246018826536 2117 40-41 -0.02580155540023199 2117 42-43 -0.5815715374993715 2117 44-45 0.7465833759863294 2117 46-47 0.11148901644968845 2117 48-49 0.7710525369562902 2117 50-51 1.0949049187066677 2117 52-53 1.5990289147083878 2117 54-55 -0.9721197380897415 2117 56-57 -0.15950167206310084 2117 58-59 0.041542729046248894 2117 60-61 1.2430331011071232 2117 62-63 0.2694122561549186 2117 64-65 1.1163149302327362 2117 66-67 -2.1719147142155215 2117 68-69 -0.2864618394492986 2117 70-71 0.87647508528255 2117 72-73 1.2912786920576593 2117 74-75 0.4891376318865497 2117 76 -1.1586325322412527 2118 1 1.8431610118959618 2118 2 1.9175927083538227 2118 3 0.18501913732018238 2118 4 1.1302580205768962 2118 5 0.5858251721925747 2118 6 1.3589349935252493 2118 7 0.21423744850167026 2118 8 -2.081990613852021 2118 9 -0.6533919225800204 2118 10-11 -0.6401314870400334 2118 12-13 0.8891152808563021 2118 14-15 -0.5424568839721182 2118 16-17 0.5107714824319203 2118 18-19 -0.14890349313830953 2118 20-21 -3.212944864400992 2118 22-23 -0.46705638024161544 2118 24-25 -0.05322767141463203 2118 26-27 0.978405117016468 2118 28-29 -1.014758563785275 2118 30-31 -0.594257476674418 2118 32-33 0.7000976518814568 2118 34-35 1.8326742476608615 2118 36-37 0.44299902552487325 2118 38-39 0.09202888007986587 2118 40-41 -0.9571101804288276 2118 42-43 -0.1595285267466835 2118 44-45 0.4731905596971515 2118 46-47 -0.9844272498509312 2118 48-49 -0.6159870420874078 2118 50-51 -0.4599452300007201 2118 52-53 -0.869799728622656 2118 54-55 1.2654689143925282 2118 56-57 1.632822738826853 2118 58-59 1.0145466183164373 2118 60-61 -1.496157015570983 2118 62-63 1.314539229387588 2118 64-65 -0.30235356667872537 2118 66-67 -3.0071354874939438 2118 68-69 1.1559426446504482 2118 70-71 -0.8842204077396438 2118 72-73 -0.9777672928428203 2118 74-75 0.4338299219803545 2118 76 1.3842638301472157 2119 1 0.8051425787162394 2119 2 1.4782685916103517 2119 3 1.428874744079014 2119 4 0.07227030936337897 2119 5 1.2179296268776731 2119 6 2.368535607964578 2119 7 -2.440524456260235 2119 8 2.115398019020482 2119 9 1.0098185228884908 2119 10-11 0.5766503869999049 2119 12-13 0.09072818408210992 2119 14-15 0.00516216364692923 2119 16-17 0.9618083487913687 2119 18-19 0.6907662457249799 2119 20-21 -0.5564548490400121 2119 22-23 1.673112590572515 2119 24-25 0.7699290106130157 2119 26-27 1.3094343028843625 2119 28-29 2.28170841010105 2119 30-31 1.5664568090398703 2119 32-33 1.5437996488092622 2119 34-35 1.5799860756178532 2119 36-37 -0.5264710205580769 2119 38-39 0.9215219676374673 2119 40-41 0.9549481913069648 2119 42-43 0.2160475101196795 2119 44-45 0.4816391004037577 2119 46-47 0.5019398776452277 2119 48-49 -1.3533142310275004 2119 50-51 2.166206843731999 2119 52-53 -0.19871677931390153 2119 54-55 0.33094510486872153 2119 56-57 2.5114709723137985 2119 58-59 2.79027626501383 2119 60-61 2.553190142647143 2119 62-63 0.2749846978975725 2119 64-65 2.2131994287129793 2119 66-67 1.8429029149638119 2119 68-69 -0.26936457501161115 2119 70-71 0.900917841108285 2119 72-73 -2.1933970931500397 2119 74-75 0.5970403674488622 2119 76 2.2924819561072773 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 21.0 13 227.0 14 535.0 15 852.0 16 1099.0 17 1314.0 18 1317.0 19 1363.0 20 1231.0 21 956.0 22 864.0 23 714.0 24 704.0 25 646.0 26 775.0 27 780.0 28 995.0 29 1095.0 30 1307.0 31 1584.0 32 1932.0 33 2366.0 34 2930.0 35 3974.0 36 5584.0 37 3901.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.08288900909255 21.29407825258223 14.746413208664965 29.876619529660253 2 18.21951469233132 22.665608682297535 29.113340841609503 30.001535783761646 3 19.732261697552985 23.589638578888092 28.028053650046076 28.650046073512847 4 11.247056414456845 25.289239275110063 31.862393774956487 31.601310535476607 5 10.65833930582574 32.9528002457254 31.020272345653733 25.368588102795126 6 25.399303778028052 34.91604382103 17.449063171905397 22.235589229036552 7 22.302139858707896 30.636326405242144 18.378212347701446 28.68332138834852 8 19.190109299408707 32.759106150971405 17.728517674763864 30.32226687485602 9 20.45152042592403 21.631514282788984 17.774137401453878 40.14282788983311 10-11 19.856404218286063 26.877495648612676 25.422340534452747 27.84375959864851 12-13 20.123886556772806 24.73379748131463 25.111344322719358 30.0309716391932 14-15 19.545408006552677 26.19791133408416 22.825586157469026 31.431094501894137 16-17 20.9596088870687 28.17395310740248 23.674106685778643 27.192331319750178 18-19 19.357274495751 27.336950957305213 24.189874065731544 29.115900481212247 20-21 18.550988020886656 27.718337258114055 24.808027029794204 28.92264769120508 22-23 21.787652298556363 27.21280843657213 23.740657315449983 27.25888194942152 24-25 19.91911538855329 30.100081908467285 23.274802907750587 26.70599979522883 26-27 18.55226784068803 30.464830551858295 25.014078017815088 25.96882358963858 28-29 20.342735742807413 27.697860141292107 27.20640933756527 24.75299477833521 30-31 19.622197194634996 28.14195761236818 26.889014026825027 25.346831166171803 32-33 18.556107300092147 29.63806696017201 25.952185932220743 25.8536398075151 34-35 19.037319545408007 29.277157776185113 25.83316269069315 25.85235998771373 36-37 19.50829323231289 27.70041978089485 26.19791133408416 26.5933756527081 38-39 19.16274188594246 26.681683219002768 28.024214190641956 26.13136070441282 40-41 18.44732261697553 26.77638988430429 27.183372581140574 27.592914917579602 42-43 17.20589740964472 26.45771475376267 28.383843554827482 27.952544281765128 44-45 17.507934882768506 26.45771475376267 28.719156342786935 27.315194020681886 46-47 17.410668577864236 26.95812429609911 28.45423364390294 27.176973482133715 48-49 16.960172007781303 27.93334698474455 27.57755707996314 27.528923927511006 50-51 16.565987508958738 27.256322309818774 27.395822668168325 28.78186751305416 52-53 16.10525238046483 27.58267635916863 27.425258523599876 28.886812736766665 54-55 15.819852564758882 27.701699600696223 26.708559434831574 29.769888399713317 56-57 16.98192894440463 27.919268966929454 26.466673492372273 28.632128596293644 58-59 16.75540083956179 27.159056004914504 26.44235691614621 29.643186239377496 60-61 16.49687723968465 26.12624142520733 26.563939797276543 30.812941537831474 62-63 17.12526876215829 26.737995290263132 26.292617999385687 29.844117948192896 64-65 17.42730623528207 27.495648612675332 24.529026313095116 30.548018838947478 66-67 16.979369304801885 28.78570697245828 23.474454796764615 30.76046892597522 68-69 17.001126241425208 28.226425719258728 23.837923620354253 30.93452441896181 70-71 17.935394696426744 27.874475273881437 24.11052523804648 30.079604791645338 72-73 18.15552370226272 26.982440872325174 24.068291184601208 30.793744240810895 74-75 19.096191256271116 26.620251868536908 24.138681273676667 30.14487560151531 76 19.571004402580115 24.938568649534147 25.089587386096035 30.4008395617897 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 2.0 9 1.5 10 1.5 11 1.5 12 1.0 13 0.5 14 1.0 15 1.0 16 4.0 17 8.0 18 8.0 19 7.5 20 13.5 21 20.0 22 23.5 23 29.0 24 45.0 25 59.0 26 80.5 27 114.0 28 148.5 29 171.0 30 202.5 31 273.0 32 362.5 33 413.0 34 517.5 35 729.0 36 933.5 37 1031.0 38 1173.0 39 1507.5 40 1839.0 41 2073.0 42 2168.0 43 2295.5 44 2473.0 45 2616.5 46 2710.0 47 2727.5 48 2729.5 49 2607.5 50 2501.0 51 2375.0 52 2102.5 53 1818.0 54 1680.0 55 1583.0 56 1361.0 57 1104.5 58 973.0 59 860.0 60 649.0 61 471.0 62 391.0 63 327.0 64 250.0 65 190.5 66 119.0 67 94.0 68 78.5 69 56.5 70 45.0 71 40.0 72 32.5 73 22.0 74 18.5 75 18.0 76 13.0 77 7.0 78 5.0 79 4.0 80 6.5 81 6.0 82 2.0 83 1.0 84 1.5 85 2.0 86 2.0 87 2.0 88 1.5 89 2.5 90 2.5 91 2.0 92 3.0 93 1.5 94 0.5 95 1.5 96 2.0 97 1.5 98 1.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6271117026722638 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0025596396027439336 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 39068.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.50824203952084 #Duplication Level Percentage of deduplicated Percentage of total 1 88.30344827586207 73.74065731545 2 7.399233716475095 12.35794000204771 3 2.418390804597701 6.0586669396948905 4 0.9563218390804598 3.1944302242244293 5 0.4842911877394636 2.0221152861677076 6 0.20229885057471264 1.0136172826865977 7 0.1103448275862069 0.6450291798914712 8 0.05517241379310345 0.36858810279512644 9 0.02145593869731801 0.1612572949728678 >10 0.049042145593869726 0.4376983720692127 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007678918808231801 0.0 2 0.0 0.0 0.0 0.007678918808231801 0.0 3 0.0 0.0 0.0 0.007678918808231801 0.0 4 0.0 0.0 0.0 0.010238558410975734 0.0 5 0.0 0.0 0.0 0.010238558410975734 0.0 6 0.0 0.0 0.0 0.010238558410975734 0.0 7 0.0 0.0 0.0 0.010238558410975734 0.0 8 0.0 0.0 0.0 0.010238558410975734 0.0 9 0.0 0.0 0.0 0.017917477219207535 0.0 10 0.0 0.0 0.0 0.017917477219207535 0.0 11 0.0 0.0 0.0 0.02047711682195147 0.0 12 0.0 0.0 0.0 0.023036756424695402 0.0 13 0.0 0.0 0.0 0.023036756424695402 0.0 14 0.0 0.0 0.0 0.025596396027439336 0.0 15 0.0 0.0 0.0 0.02815603563018327 0.0 16 0.0 0.0 0.0 0.03327531483567114 0.0 17 0.0 0.0 0.0 0.03327531483567114 0.0 18 0.0 0.0 0.0 0.03583495443841507 0.0 19 0.0 0.0 0.0 0.038394594041159004 0.0 20 0.0 0.0 0.0 0.038394594041159004 0.0 21 0.0 0.0 0.0 0.04095423364390294 0.0 22 0.0 0.0 0.0 0.05119279205487867 0.0 23 0.0 0.0 0.0 0.05119279205487867 0.0 24 0.0 0.0 0.0 0.05119279205487867 0.0 25 0.0 0.0 0.0 0.05119279205487867 0.0 26 0.0 0.0 0.0 0.06399099006859835 0.0 27 0.0 0.0 0.0 0.07422954847957408 0.0 28 0.0 0.0 0.0 0.09470666530152555 0.0 29 0.0 0.0 0.0 0.11774342172622095 0.0 30 0.0 0.0 0.0 0.15101873656189208 0.0 31 0.0 0.0 0.0 0.17405549298658748 0.0 32 0.0 0.0 0.0 0.24060612265792977 0.0 33 0.0 0.0 0.0 0.30203747312378415 0.0 34 0.0 0.0 0.0 0.386505580014334 0.0 35 0.0 0.0 0.0 0.5272857581652504 0.0 36 0.0 0.0 0.0 0.657827377905191 0.0 37 0.0 0.0 0.0 0.9342684550015358 0.0 38 0.0 0.0 0.0 1.2567830449472714 0.0 39 0.0 0.0 0.0 1.6381693457561175 0.0 40 0.0 0.0 0.0 2.1065833930582576 0.0 41 0.0 0.0 0.0 2.7567318521552164 0.0 42 0.0 0.0 0.0 3.46831166171803 0.0 43 0.0 0.0 0.0 4.1747721920753555 0.0 44 0.0 0.0 0.0 4.837718849186035 0.0 45 0.0 0.0 0.0 5.523702262721409 0.0 46 0.0 0.0 0.0 6.120098290160746 0.0 47 0.0 0.0 0.0 6.7932835056824 0.0 48 0.0 0.0 0.0 7.3512849390805775 0.0 49 0.0 0.0 0.0 7.950240606122658 0.0 50 0.0 0.0 0.0 8.477526364287908 0.0 51 0.0 0.0 0.0 9.056004914508037 0.0 52 0.0 0.0 0.0 9.608887068700726 0.0 53 0.0 0.0 0.0 10.369100030715675 0.0 54 0.0 0.0 0.0 11.129312992730624 0.0 55 0.0 0.0 0.0 11.82297532507423 0.0 56 0.0 0.0 0.0 12.465444865362956 0.0 57 0.0 0.0 0.0 13.064400532405037 0.0 58 0.0 0.0 0.0 13.604484488584008 0.0 59 0.0 0.0 0.0 14.05498105866694 0.0 60 0.0 0.0 0.0 14.574587898023958 0.0 61 0.0 0.0 0.0 15.058359782942562 0.0 62 0.0 0.0 0.0 15.695710044025802 0.0 63 0.0 0.0 0.0 16.187160847752637 0.0 64 0.0 0.0 0.0 16.737483362342584 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCAAT 15 0.0022099225 69.991035 32 GGTACCG 15 0.0022099225 69.991035 26 CGAAATA 15 0.0022099225 69.991035 64 GTCTAGA 15 0.0022099225 69.991035 64 TGTACGA 15 0.0022099225 69.991035 60 GTGGTCA 15 0.0022099225 69.991035 10 TAATGTA 15 0.0022099225 69.991035 57 CTGGCAG 15 0.0022099225 69.991035 50 CACCCGG 15 0.0022099225 69.991035 13 TACCGTG 15 0.0022099225 69.991035 28 TGCAATA 15 0.0022099225 69.991035 33 CATGGTG 20 0.006905598 52.49328 42 CAGGTGG 20 0.006905598 52.49328 7 CTTTTTA 20 0.006905598 52.49328 42 TTATGGT 20 0.006905598 52.49328 22 AATATTC 20 0.006905598 52.49328 36 ATGTACG 20 0.006905598 52.49328 59 TGGCAGT 20 0.006905598 52.49328 51 ACAGGTG 20 0.006905598 52.49328 6 ATTCATG 20 0.006905598 52.49328 39 >>END_MODULE