FastQCFastQC Report
Wed 25 May 2016
SRR1780450_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780450_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39068
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT21755.567216135968056No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT18814.8146820927613385TruSeq Adapter, Index 8 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC6701.7149585338384357No Hit
CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC3230.8267635916862905No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG2420.619432783864032No Hit
TATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT1730.4428176512747005No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGT1420.36346882358963856No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC1070.2738814374936009No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC940.24060612265792974No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG840.21500972663049045No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTCTT750.191972970205795No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC680.17405549298658748No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTCTT580.14845909695914816No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG560.14333981775366028No Hit
CTTAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC540.1382205385481724No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGT510.1305416197399406No Hit
ATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGTCTT400.10238558410975734No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGTT150.002208800370.0000144
TAAGCCG150.002208800370.0000140
TAGCACC150.002208800370.0000136
GCAAACT150.002208800370.0000130
CACCATT150.002208800370.0000130
TCGTAAG150.002208800370.0000137
AGCCGTC150.002208800370.0000142
AAGCCGT150.002208800370.0000141
TTAGCAC150.002208800370.0000135
CTCGTAA150.002208800370.0000136
TTTACAC1300.059.2307742
CTTTACA1350.057.0370371
TCCACCA252.3624496E-455.99999628
AGGCGTC200.006902104252.525
TAGCAAA200.006902104252.528
ATCAGAG200.006902104252.57
CTGCGTG200.006902104252.59
TCTAATT200.006902104252.555
TCTAACT200.006902104252.563
CTAATTT200.006902104252.556
Produced by FastQC (version 0.11.5)