##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780450_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 39068 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70459199344732 34.0 34.0 34.0 31.0 34.0 2 33.18001945326098 34.0 34.0 34.0 32.0 34.0 3 33.408057745469435 34.0 34.0 34.0 33.0 34.0 4 33.41509675437698 34.0 34.0 34.0 34.0 34.0 5 33.44164021705744 34.0 34.0 34.0 34.0 34.0 6 37.191793795433604 38.0 37.0 38.0 36.0 38.0 7 36.739735845192996 38.0 37.0 38.0 34.0 38.0 8 36.80874372888297 38.0 37.0 38.0 34.0 38.0 9 36.558231800962425 38.0 37.0 38.0 34.0 38.0 10-11 36.21140063479062 38.0 37.0 38.0 33.0 38.0 12-13 36.057092761339206 38.0 37.0 38.0 32.5 38.0 14-15 36.31372222791031 38.0 37.0 38.0 33.5 38.0 16-17 36.43510033787243 38.0 37.0 38.0 34.0 38.0 18-19 36.49745315859527 38.0 37.0 38.0 34.0 38.0 20-21 36.164367257090205 38.0 37.0 38.0 33.0 38.0 22-23 36.201251663765746 38.0 36.5 38.0 32.5 38.0 24-25 35.32430633766766 37.5 35.5 38.0 29.0 38.0 26-27 35.51487150609194 38.0 36.0 38.0 31.0 38.0 28-29 35.440283608067986 38.0 36.0 38.0 31.0 38.0 30-31 35.072322616975526 37.5 35.5 38.0 29.0 38.0 32-33 35.62557591891062 38.0 36.0 38.0 31.0 38.0 34-35 35.397038496979626 38.0 36.0 38.0 31.0 38.0 36-37 35.397895976246545 38.0 36.0 38.0 31.0 38.0 38-39 34.76199191153886 37.5 35.0 38.0 26.0 38.0 40-41 34.694251049452234 37.0 34.5 38.0 26.5 38.0 42-43 35.420663970512955 37.5 36.0 38.0 29.5 38.0 44-45 35.69316320262107 38.0 36.0 38.0 31.5 38.0 46-47 35.92245571823487 38.0 37.0 38.0 32.0 38.0 48-49 35.59433551755913 38.0 36.5 38.0 31.0 38.0 50-51 35.70429763489301 38.0 36.5 38.0 31.0 38.0 52-53 35.52471332036449 38.0 36.0 38.0 31.0 38.0 54-55 35.85118255349647 38.0 37.0 38.0 32.0 38.0 56-57 35.8621250127982 38.0 37.0 38.0 32.0 38.0 58-59 35.834864851028975 38.0 37.0 38.0 32.0 38.0 60-61 35.59656240401351 38.0 36.5 38.0 31.5 38.0 62-63 36.0472893416607 38.0 37.0 38.0 33.0 38.0 64-65 36.00468414047302 38.0 37.0 38.0 33.0 38.0 66-67 34.97572181836797 37.5 35.5 38.0 27.0 38.0 68-69 33.496326917170066 37.0 34.0 38.0 22.0 38.0 70-71 32.58931862393775 37.0 32.0 38.0 16.0 38.0 72-73 31.55281816320262 37.0 26.5 38.0 11.0 38.0 74-75 31.85521398587079 37.0 29.0 38.0 11.0 38.0 76 31.467850926589538 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.4408408837848157 1101 2 0.189582095319885 1101 3 0.3303438706795845 1101 4 0.39258367285368934 1101 5 0.15702935865360956 1101 6 -1.252918146830119 1101 7 -0.07295744855451858 1101 8 0.34546271164097675 1101 9 -0.26504211040042946 1101 10-11 -0.12268105705821597 1101 12-13 0.3873772483885176 1101 14-15 0.38087184878794034 1101 16-17 -0.4933233534968906 1101 18-19 -0.9593167944756402 1101 20-21 0.05490393445240471 1101 22-23 0.45286374783481875 1101 24-25 -2.8927579153442835 1101 26-27 -0.6998131058104633 1101 28-29 0.11494530678484693 1101 30-31 1.308976494932594 1101 32-33 0.09330478888551141 1101 34-35 -0.28998067761079227 1101 36-37 0.25542672894447094 1101 38-39 -0.3026659980304629 1101 40-41 0.9416641000842461 1101 42-43 0.6446821733335639 1101 44-45 0.09067306980521295 1101 46-47 0.4834298417576548 1101 48-49 -3.02516343120962 1101 50-51 -2.7127592989951594 1101 52-53 -2.3611339936931017 1101 54-55 -1.5851169454952796 1101 56-57 -1.6026747230880716 1101 58-59 -1.7923715326330907 1101 60-61 -2.56936464643573 1101 62-63 -1.1244636029790982 1101 64-65 -1.5799369133022907 1101 66-67 -3.120672882927458 1101 68-69 -2.255567565891525 1101 70-71 -2.6299269630243103 1101 72-73 -3.755607215994356 1101 74-75 -2.3412975648796888 1101 76 -3.0176260244794513 1102 1 -0.5512968586275875 1102 2 0.24756165506831707 1102 3 0.1860828643902792 1102 4 0.4008383898348242 1102 5 0.19240671714417346 1102 6 0.5002265072579348 1102 7 0.021578714967496637 1102 8 0.5034815795655021 1102 9 0.4000522292222115 1102 10-11 0.9405068360235518 1102 12-13 1.1016068081369355 1102 14-15 0.2666815972156158 1102 16-17 0.08578435090562664 1102 18-19 -0.29097953032469803 1102 20-21 0.5869400979744199 1102 22-23 0.6776082446901697 1102 24-25 -4.474715462514091 1102 26-27 -1.1726904643010272 1102 28-29 0.5999099294263601 1102 30-31 1.8669560546810189 1102 32-33 0.6567874932880216 1102 34-35 0.9248385047791388 1102 36-37 -1.6691998433825788 1102 38-39 0.009834001969537098 1102 40-41 1.4274148862477745 1102 42-43 1.2422647205033712 1102 44-45 0.9347178811259695 1102 46-47 1.3800493386130057 1102 48-49 -3.4362286827819446 1102 50-51 -0.33834892163667263 1102 52-53 0.3363895912125585 1102 54-55 1.1085740922405662 1102 56-57 1.3324667863458899 1102 58-59 0.33439733529143467 1102 60-61 -6.5402765961212594 1102 62-63 -2.30400369731872 1102 64-65 0.28612047663481377 1102 66-67 1.4841816768209668 1102 68-69 -0.37919177972799645 1102 70-71 -1.3277453592507271 1102 72-73 -5.2640584738559895 1102 74-75 -1.818007470540067 1102 76 0.3164934723758961 1103 1 0.76332578288185 1103 2 0.6041654286532179 1103 3 0.0345105373462502 1103 4 0.49675033952035363 1103 5 0.16536269198694242 1103 6 0.13041518650321393 1103 7 0.7957925514454871 1103 8 0.8787960449743082 1103 9 0.518291222932902 1103 10-11 0.26377727627512115 1103 12-13 -0.8126227516114852 1103 14-15 -0.5212114845453897 1103 16-17 0.3827183131697822 1103 18-19 0.7583915388576941 1103 20-21 -0.12217939888092388 1103 22-23 0.576822081168153 1103 24-25 -3.278174582010948 1103 26-27 -0.8164797724771304 1103 28-29 -0.015263026548481662 1103 30-31 0.8235598282659211 1103 32-33 0.6995547888855072 1103 34-35 0.18710265572253348 1103 36-37 -1.2039482710555376 1103 38-39 -0.4859993313638 1103 40-41 -0.37187756658241256 1103 42-43 -0.04177615999977036 1103 44-45 1.25838140313855 1103 46-47 0.8042631750909948 1103 48-49 -2.611621764542953 1103 50-51 -0.6273426323284923 1103 52-53 -0.2298839936931003 1103 54-55 -0.9111586121619482 1103 56-57 0.005658610245262707 1103 58-59 -0.24445486596642496 1103 60-61 -4.427697979769057 1103 62-63 -1.148421936312431 1103 64-65 0.41693808669771215 1103 66-67 -1.4508812162607896 1103 68-69 -0.274317565891522 1103 70-71 -0.43096862969097316 1103 72-73 -4.836857215994357 1103 74-75 -3.348589231546356 1103 76 -4.384292691146118 1104 1 0.17165911621518148 1104 2 0.48749876198655073 1104 3 0.31784387067958875 1104 4 -0.019916327146312085 1104 5 -0.9013039746797205 1104 6 -0.052918146830116086 1104 7 0.2541258847788157 1104 8 0.6287960449743082 1104 9 1.076624556266239 1104 10-11 -0.9612227237248732 1104 12-13 -0.24595608494482235 1104 14-15 0.8496218487879474 1104 16-17 -0.2381150201635478 1104 18-19 0.5458915388576884 1104 20-21 -0.10967939888092815 1104 22-23 0.330988747834823 1104 24-25 0.2801587513223822 1104 26-27 1.2293535608562038 1104 28-29 1.4680703067848455 1104 30-31 0.7568931615992582 1104 32-33 0.2328881222188386 1104 34-35 0.3121026557225335 1104 36-37 1.1127183956111324 1104 38-39 1.7473340019695343 1104 40-41 1.590622433417579 1104 42-43 1.0248905066668996 1104 44-45 0.570881403138543 1104 46-47 1.1292631750909976 1104 48-49 0.35087823545705277 1104 50-51 0.44765736767150344 1104 52-53 0.6576160063068954 1104 54-55 0.4763413878380547 1104 56-57 0.2139919435785913 1104 58-59 -0.927788199299755 1104 60-61 0.27230202023094563 1104 62-63 0.1307447303542304 1104 64-65 0.5961047533643793 1104 66-67 -0.009214549594119603 1104 68-69 1.7340157674418109 1104 70-71 1.8398647036423554 1104 72-73 1.0839761173389775 1104 74-75 0.3347441017869741 1104 76 -0.10929269114611628 1105 1 0.9208044153604789 1105 2 0.5392081636959531 1105 3 0.40459600743172075 1105 4 -0.09513000235998703 1105 5 -0.22865440203015197 1105 6 0.03554339163142117 1105 7 0.8188694745224083 1105 8 -0.4889389977607337 1105 9 1.0490604537021326 1105 10-11 0.2428371053349494 1105 12-13 -0.25418258067131205 1105 14-15 0.3341303957964925 1105 16-17 0.2362439541954231 1105 18-19 0.4908701713363257 1105 20-21 0.29876077205923934 1105 22-23 0.11165114099721762 1105 24-25 0.6470390932027286 1105 26-27 0.8936698001724466 1105 28-29 0.6124079136224481 1105 30-31 1.201337606043694 1105 32-33 0.7196402589709834 1105 34-35 -1.6962306776107923 1105 36-37 0.81378677167951 1105 38-39 0.21282545496099203 1105 40-41 0.5940412368363894 1105 42-43 0.8683734126498024 1105 44-45 0.7593429416000887 1105 46-47 1.014946935774752 1105 48-49 -0.06536108078226732 1105 50-51 0.8970163420304829 1105 52-53 0.371504895195784 1105 54-55 0.4898029262995962 1105 56-57 0.4106799777666268 1105 58-59 0.23513915112759065 1105 60-61 0.7778575757864914 1105 62-63 -0.026306551697047098 1105 64-65 0.6442885140481351 1105 66-67 0.4211273307477583 1105 68-69 -1.0672662838402402 1105 70-71 0.506958720736371 1105 72-73 0.5496812455441074 1105 74-75 -0.6555336759907995 1105 76 0.6009637191102968 1106 1 -1.52167421711815 1106 2 0.2141654286532173 1106 3 0.2711772040129219 1106 4 -0.06991632714630924 1106 5 0.18202935865360814 1106 6 -0.019584813496784648 1106 7 -1.1008741152211812 1106 8 -0.03953728835902126 1106 9 -0.9750421104004303 1106 10-11 0.46211060960845884 1106 12-13 1.328210581721848 1106 14-15 -0.1595448178787251 1106 16-17 0.4902183131697768 1106 18-19 0.7300582055243581 1106 20-21 0.10448726778574269 1106 22-23 0.37515541450148504 1106 24-25 0.3684920846557205 1106 26-27 0.9626868941895381 1106 28-29 0.9105703067848481 1106 30-31 0.4635598282659217 1106 32-33 0.236221455552176 1106 34-35 0.9821026557225352 1106 36-37 0.9893850622778046 1106 38-39 -0.48516599803046745 1106 40-41 1.1706224334175843 1106 42-43 0.7390571733335634 1106 44-45 1.2275480698052164 1106 46-47 0.7625965084243234 1106 48-49 1.5325449021237105 1106 50-51 -0.3290092989951532 1106 52-53 0.7176160063068977 1106 54-55 -0.4069919454952853 1106 56-57 0.1798252769119273 1106 58-59 0.8963784673669082 1106 60-61 1.1839686868976074 1106 62-63 1.0274113970208987 1106 64-65 -1.3097285799689544 1106 66-67 0.160785450405875 1106 68-69 1.2690157674418074 1106 70-71 -0.30680196302430573 1106 72-73 -1.435190549327686 1106 74-75 -1.6794225648796868 1106 76 -0.5259593578127841 1107 1 0.40499244954851576 1107 2 -0.5375012380134478 1107 3 0.0345105373462502 1107 4 0.19675033952035648 1107 5 -0.03463730801306042 1107 6 0.18874851983655105 1107 7 0.5874592181121514 1107 8 0.16212937830764673 1107 9 -0.41504211040042804 1107 10-11 0.2346106096084526 1107 12-13 -0.1292894182781552 1107 14-15 -0.004544817878723961 1107 16-17 0.6285516465031122 1107 18-19 0.8500582055243626 1107 20-21 0.7778206011190747 1107 22-23 -0.2523445854985127 1107 24-25 0.3926587513223794 1107 26-27 -0.9748131058104619 1107 28-29 0.6180703067848441 1107 30-31 0.10272649493258967 1107 32-33 0.9453881222188443 1107 34-35 -0.09623067761079795 1107 36-37 1.4210517289444624 1107 38-39 -0.3484993313637972 1107 40-41 0.12812243341758034 1107 42-43 0.6790571733335611 1107 44-45 1.00838140313855 1107 46-47 0.8250965084243234 1107 48-49 1.0925449021237128 1107 50-51 1.0351573676715091 1107 52-53 1.061782672973564 1107 54-55 0.7263413878380547 1107 56-57 0.9431586102452627 1107 58-59 0.15554513403358072 1107 60-61 0.8931353535642685 1107 62-63 0.8849113970209075 1107 64-65 0.6544380866977164 1107 66-67 0.18245211707254327 1107 68-69 1.2798491007751451 1107 70-71 -1.164301963024311 1107 72-73 -1.0368572159943525 1107 74-75 -2.2735892315463566 1107 76 -0.44262602447944843 1108 1 -0.8896403188130684 1108 2 0.312640004924404 1108 3 -5.177112413150553E-4 1108 4 0.45296502878588996 1108 5 0.11677512136547108 1108 6 0.1270253559947392 1108 7 -0.16917919996694764 1108 8 -0.16089322056241429 1108 9 0.15444941502330067 1108 10-11 0.7598224740152375 1108 12-13 1.2217699037557423 1108 14-15 0.34926874144331066 1108 16-17 -0.0022393139488627867 1108 18-19 0.7114988834904636 1108 20-21 0.9343177762603148 1108 22-23 -0.10637000922732653 1108 24-25 1.0749327626218204 1108 26-27 -0.4445164956409684 1108 28-29 -0.7232432525371877 1108 30-31 0.44050898080829626 1108 32-33 0.7043570487725148 1108 34-35 0.7684585879259274 1108 36-37 0.9094698080405124 1108 38-39 0.5945797646813986 1108 40-41 0.8202834503667304 1108 42-43 -0.108230962259654 1108 44-45 0.8663616291272476 1108 46-47 0.8375965084243262 1108 48-49 1.2607652411067676 1108 50-51 0.5187025654116226 1108 52-53 -0.46653653606598766 1108 54-55 -0.6913139793935841 1108 56-57 0.19711341250514636 1108 58-59 0.6149377894008055 1108 60-61 1.2872737716433633 1108 62-63 1.0622419054954761 1108 64-65 0.7155256573191835 1108 66-67 0.7399379927787564 1108 68-69 1.560286953882489 1108 70-71 -0.1259545053971891 1108 72-73 -0.9740041086497229 1108 74-75 -1.607981886913585 1108 76 -1.0776542730670222 1109 1 -1.589722997605957 1109 2 0.28384022540118536 1109 3 0.4467056592974714 1109 4 0.18170968911385188 1109 5 0.25560659442597 1109 6 -0.17771489479759595 1109 7 -0.10420744855451858 1109 8 0.5619261262751252 1109 9 0.883331873339408 1109 10-11 0.5194683331856922 1109 12-13 0.6073975735917685 1109 14-15 0.6041950195196506 1109 16-17 0.5992833538201836 1109 18-19 0.5575785307276107 1109 20-21 0.7294466173792387 1109 22-23 0.2388139510868612 1109 24-25 -0.3208575088402199 1109 26-27 0.5161421787423848 1109 28-29 -1.0838605875241 1109 30-31 0.15120210468869288 1109 32-33 1.111343406771688 1109 34-35 -0.17184043370836122 1109 36-37 1.445848476911948 1109 38-39 1.17853318896141 1109 40-41 0.32720779927123544 1109 42-43 1.007105953821366 1109 44-45 0.737141565740167 1109 46-47 1.0103607360666018 1109 48-49 0.9988457151318428 1109 50-51 0.17062484734630345 1109 52-53 -0.5051888717418791 1109 54-55 -0.36573178289365416 1109 56-57 0.33990657772493194 1109 58-59 0.2058500120823581 1109 60-61 0.4092938901496339 1109 62-63 0.003712210029028995 1109 64-65 1.0421413387302323 1109 66-67 0.8659887024384005 1109 68-69 -0.4143582162980266 1109 70-71 0.28661267112202893 1109 72-73 0.14098831246092658 1109 74-75 -0.3086502071561128 1109 76 0.3858292600733968 1110 1 -0.8378878093511624 1110 2 -0.6133102995021176 1110 3 -0.4764926988997047 1110 4 -0.28399399704922246 1110 5 0.3087283877798228 1110 6 -0.3540508329142611 1110 7 0.49126180710891276 1110 8 0.6864821291167047 1110 9 0.9509773070752985 1110 10-11 -0.10240395349833875 1110 12-13 0.8014144652169932 1110 14-15 1.008804696684379 1110 16-17 0.551528992781428 1110 18-19 -0.13999033816496365 1110 20-21 1.1003125105041818 1110 22-23 -0.3915436146247231 1110 24-25 -1.2061681095190409 1110 26-27 1.4585606805973015 1110 28-29 0.5880945786295086 1110 30-31 -0.09585764746223191 1110 32-33 0.8676777662317932 1110 34-35 0.4671997431011832 1110 36-37 1.3907928292680936 1110 38-39 1.3909019631345814 1110 40-41 -0.8113193141552344 1110 42-43 0.5805134840131743 1110 44-45 1.3341500115527865 1110 46-47 0.8254605860942235 1110 48-49 0.9709429603761421 1110 50-51 -0.472164638800983 1110 52-53 1.1136839674719425 1110 54-55 -0.1392734988933384 1110 56-57 0.9331262477857081 1110 58-59 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48-49 -0.24179575721694135 2116 50-51 -0.892360947346809 2116 52-53 0.5375610612519495 2116 54-55 -0.255618319121659 2116 56-57 -0.34511977803312277 2116 58-59 -3.162907246918806 2116 60-61 -2.017624719695796 2116 62-63 -0.1050611304516238 2116 64-65 -1.5046736349140062 2116 66-67 -0.19547828585785965 2116 68-69 0.40159818502423406 2116 70-71 1.0429782567559087 2116 72-73 -0.025410329547469246 2116 74-75 0.36766534720822364 2116 76 -0.3880472699006958 2117 1 0.958538548839293 2117 2 0.4850164924830054 2117 3 0.09053890614057991 2117 4 -0.9199163271463107 2117 5 -0.13818340730383483 2117 6 0.021904548205341712 2117 7 0.17593439541711575 2117 8 0.05024994568353236 2117 9 -2.1427016848685128 2117 10-11 -1.040123432944739 2117 12-13 -2.352427716150494 2117 14-15 -0.21465120085744616 2117 16-17 -1.0909518995961776 2117 18-19 -1.7196226455394736 2117 20-21 -0.12040634923553739 2117 22-23 -0.04569564932830161 2117 24-25 -2.781720681301728 2117 26-27 -2.533589701555144 2117 28-29 -2.026344586832174 2117 30-31 -0.4806954908830079 2117 32-33 -2.0359062040222966 2117 34-35 0.9083792514672169 2117 36-37 -0.9700830228285824 2117 38-39 0.5311105977142176 2117 40-41 0.33317562490694286 2117 42-43 0.17161036482292502 2117 44-45 -0.5433029940245788 2117 46-47 -2.130488597958653 2117 48-49 -1.0239444595784093 2117 50-51 -0.4301795117611178 2117 52-53 0.44538196375370376 2117 54-55 -0.5628430093250714 2117 56-57 0.3055699577629909 2117 58-59 -0.501813021994792 2117 60-61 -0.5897547173577138 2117 62-63 -0.3169503051067579 2117 64-65 -1.6784519842242744 2117 66-67 -1.406023060232421 2117 68-69 0.7978455546758525 2117 70-71 1.1795810156990925 2117 72-73 2.0686392379063534 2117 74-75 2.4928114776735057 2117 76 2.077232131548918 2118 1 -0.442857012817079 2118 2 0.5891116652123571 2118 3 -0.9041991400731035 2118 4 0.36502990941282576 2118 5 0.316975595212746 2118 6 0.4433184123096652 2118 7 0.8635344869293533 2118 8 0.629064862178609 2118 9 -1.7946120028735493 2118 10-11 -0.5324592828646644 2118 12-13 -1.1993162999985856 2118 14-15 0.6527132466374042 2118 16-17 -2.7757494287657067 2118 18-19 -0.42655469770144805 2118 20-21 -0.17325466769813147 2118 22-23 0.9174134790176183 2118 24-25 1.009459826591204 2118 26-27 -1.8886571918319675 2118 28-29 -0.5159350695592408 2118 30-31 -0.8345046878631095 2118 32-33 -0.10528392079191207 2118 34-35 -0.11020917223445537 2118 36-37 -2.5192708517006963 2118 38-39 0.19531787293728087 2118 40-41 0.5002998527724216 2118 42-43 1.005401259355068 2118 44-45 -2.703849779657155 2118 46-47 0.2542631750909976 2118 48-49 -0.41041208712359634 2118 50-51 -0.7171275785650479 2118 52-53 -1.6034054990694457 2118 54-55 -0.82285216054904 2118 56-57 0.43563172852482523 2118 58-59 -0.35184733908470633 2118 60-61 -0.8099560442851939 2118 62-63 -0.7765133341618977 2118 64-65 0.30833593616008415 2118 66-67 0.20207577298652524 2118 68-69 -1.8331885336334572 2118 70-71 1.2210474993412817 2118 72-73 2.4160997732529594 2118 74-75 1.1867602308192389 2118 76 1.5778040830474325 2119 1 0.7764210209770894 2119 2 -1.8386917142039216 2119 3 -0.11310851027279512 2119 4 -0.8722972795272597 2119 5 -0.20606587944163124 2119 6 -0.5505371944491628 2119 7 -0.9732550676021319 2119 8 -0.10096585978759265 2119 9 -0.6221849675432836 2119 10-11 -0.31241319991535477 2119 12-13 -0.2465513230400589 2119 14-15 0.17997899164508624 2119 16-17 -0.4583531154016498 2119 18-19 -0.6177989373327861 2119 20-21 -1.9138460655475953 2119 22-23 -0.08508268073660474 2119 24-25 0.5075397037033369 2119 26-27 0.3805440370466755 2119 28-29 -0.08371540750086837 2119 30-31 0.9592741139802072 2119 32-33 0.6662214555521757 2119 34-35 0.7025788461987261 2119 36-37 0.21748030037304034 2119 38-39 -1.5080231408876017 2119 40-41 -0.8165204237252794 2119 42-43 0.3790571733335639 2119 44-45 0.6554052126623517 2119 46-47 0.6352155560433772 2119 48-49 -0.32650271692390476 2119 50-51 0.9940859391000814 2119 52-53 -0.07095542226453233 2119 54-55 0.10610329259996121 2119 56-57 -1.0937461516595022 2119 58-59 -0.4188596278711856 2119 60-61 0.36515916308808016 2119 62-63 -1.0002076505981492 2119 64-65 -0.08068096092133459 2119 66-67 0.5110235456439725 2119 68-69 0.888777672203716 2119 70-71 1.8112932750709305 2119 72-73 2.6476665935294506 2119 74-75 1.7948631494060265 2119 76 2.297850165996742 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 4.0 22 4.0 23 14.0 24 48.0 25 72.0 26 159.0 27 278.0 28 424.0 29 699.0 30 1081.0 31 1612.0 32 2304.0 33 3331.0 34 4465.0 35 5660.0 36 7553.0 37 11360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.95617897000102 21.554725094706665 10.778642367154704 33.7104535681376 2 17.820210914303267 18.327019555646565 43.339817753660284 20.512951776389883 3 15.910719770656293 26.128801064810077 37.063581447732155 20.896897716801472 4 11.285451008498002 23.697143442203338 34.107197706562914 30.910207842735744 5 10.617385072181836 38.873246646872126 37.02262721408825 13.486741066857785 6 21.234770144363672 39.06010033787243 16.8782635404935 22.8268659772704 7 21.78509265895362 35.98597317497696 18.951571618716084 23.277362547353334 8 19.448141701648407 36.29824920651173 23.251766151325892 21.001842940513978 9 18.931094501894133 20.512951776389883 17.564246954028874 42.99170676768711 10-11 18.745520630695196 25.57847855022013 30.842377393263025 24.833623425821642 12-13 22.60417733183168 19.421265485819596 27.883434012491044 30.091123169857685 14-15 22.994522371250127 23.160898945428485 20.67932835056824 33.165250332753146 16-17 24.55206306951981 27.67482338486741 20.365772499232108 27.40734104638067 18-19 22.76799426640729 26.28877853998157 20.78427357428074 30.158953619330397 20-21 20.870021500972662 24.059332445991604 20.86618204156855 34.20446401146718 22-23 23.049554622709124 30.317651274700523 21.068393570185318 25.564400532405035 24-25 24.534145592300604 32.187468004504964 20.78427357428074 22.49411282891369 26-27 29.832599569980545 28.934166069417426 21.074792669192178 20.158441691409852 28-29 29.66494317600082 22.419883280434114 21.83244599160438 26.082727551960684 30-31 25.819084672878056 21.09015050680864 25.18941333060305 27.901351489710247 32-33 26.323333674618617 25.11006450291799 27.027234565373192 21.539367257090202 34-35 24.137401453875295 27.23200573359271 26.265741783556873 22.364851028975117 36-37 18.75959864851029 32.41271628954643 26.90053240503737 21.927152656905907 38-39 20.415685471485613 24.02989659056005 28.16115490938876 27.393263028565578 40-41 19.37007269376472 23.994061636121632 31.491246032558617 25.14461963755503 42-43 25.255963960274393 22.629773727859117 32.531739531074024 19.582522780792463 44-45 19.2932835056824 25.531125217569368 29.88123272243268 25.294358554315554 46-47 25.614313504658544 20.285143851745673 27.431657622606735 26.66888502098905 48-49 22.257346165659875 20.001023855841098 29.135097778232826 28.606532200266205 50-51 16.68501075048633 20.56926384765025 34.22366130848776 28.522064093375654 52-53 18.42172622094809 20.57438312685574 38.72862700931709 22.275263642879082 54-55 16.837309306849598 20.566704208047508 34.92628237944098 27.669704105661925 56-57 23.07387119893519 20.564144568444764 30.149994880720794 26.211989351899252 58-59 17.546329476809667 27.443176000819086 33.84227500767892 21.168219514692332 60-61 19.686188184703592 35.46892597522269 24.49191153885533 20.35297430121839 62-63 14.912460325586158 42.48745776594656 22.172878058769324 20.42720384969796 64-65 14.661615644517251 43.97204873553804 21.072233029589434 20.29410259035528 66-67 14.697450598955667 42.82277055390601 20.25698781611549 22.222791031022833 68-69 14.79599672366131 40.661154909388756 20.733080782225862 23.80976758472407 70-71 15.41542950752534 38.335722330295894 21.402426538343402 24.84642162383536 72-73 16.44312480802703 34.758625985461244 21.59695914815194 27.201290058359785 74-75 17.02800245725402 34.08544076993959 21.298761134432272 27.587795638374118 76 17.60776082727552 34.70103409439951 21.12982492065117 26.561380157673796 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 1.0 17 2.0 18 3.5 19 5.0 20 8.0 21 11.0 22 13.5 23 23.5 24 40.5 25 50.0 26 59.0 27 97.0 28 141.0 29 156.0 30 192.5 31 274.0 32 367.5 33 416.0 34 489.5 35 641.5 36 818.0 37 916.0 38 1022.0 39 1313.0 40 1667.5 41 2009.5 42 2182.0 43 2770.5 44 3900.0 45 4393.0 46 4345.0 47 4055.0 48 3308.0 49 2554.0 50 2257.0 51 2027.0 52 1584.0 53 1251.0 54 1131.0 55 1058.5 56 885.5 57 689.0 58 593.0 59 499.5 60 361.5 61 239.5 62 162.0 63 135.0 64 89.0 65 49.5 66 18.5 67 8.0 68 6.5 69 3.0 70 0.5 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 39068.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.828401761032055 #Duplication Level Percentage of deduplicated Percentage of total 1 70.93851446291004 37.47568342377393 2 16.735306943165853 17.681990375755095 3 6.075875769174863 9.629364185522677 4 2.6406318135568583 5.580014333981776 5 1.3518096807015842 3.570697245827788 6 0.7267793982266583 2.3036756424695404 7 0.4602936188768836 1.7021603358247157 8 0.3052473472551965 1.2900583597829425 9 0.2228790154561752 1.0596907955359887 >10 0.4651388148650613 3.701238865567728 >50 0.038761567905421776 1.3822053854817242 >100 0.024225979940888608 2.5263642879082626 >500 0.004845195988177722 1.7149585338384357 >1k 0.009690391976355444 10.381898228729394 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT 2175 5.567216135968056 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTT 1881 4.8146820927613385 TruSeq Adapter, Index 8 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC 670 1.7149585338384357 No Hit CTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC 323 0.8267635916862905 No Hit TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG 242 0.619432783864032 No Hit TATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCT 173 0.4428176512747005 No Hit CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGT 142 0.36346882358963856 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC 107 0.2738814374936009 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC 94 0.24060612265792974 No Hit CTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG 84 0.21500972663049045 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAATATCGTATGCCGTCTT 75 0.191972970205795 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC 68 0.17405549298658748 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAAACTCGTATGCCGTCTT 58 0.14845909695914816 No Hit CTTATAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG 56 0.14333981775366028 No Hit CTTAACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTC 54 0.1382205385481724 No Hit CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGT 51 0.1305416197399406 No Hit ATACACATCTCCGAGCCCACGAGACGGCGTCGAACCTCGTATGCCGTCTT 40 0.10238558410975734 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007678918808231801 0.0 2 0.0 0.0 0.0 0.007678918808231801 0.0 3 0.0 0.0 0.0 0.007678918808231801 0.0 4 0.0 0.0 0.0 0.010238558410975734 0.0 5 0.0 0.0 0.0 0.010238558410975734 0.0 6 0.0 0.0 0.0 0.010238558410975734 0.0 7 0.0 0.0 0.0 0.012798198013719668 0.0 8 0.0 0.0 0.0 0.012798198013719668 0.0 9 0.0 0.0 0.0 0.02047711682195147 0.0 10 0.0 0.0 0.0 0.02047711682195147 0.0 11 0.0 0.0 0.0 0.023036756424695402 0.0 12 0.0 0.0 0.0 0.025596396027439336 0.0 13 0.0 0.0 0.0 0.025596396027439336 0.0 14 0.0 0.0 0.0 0.025596396027439336 0.0 15 0.0 0.0 0.0 0.02815603563018327 0.0 16 0.0 0.0 0.0 0.03327531483567114 0.0 17 0.0 0.0 0.0 0.03327531483567114 0.0 18 0.0 0.0 0.0 0.038394594041159004 0.0 19 0.0 0.0 0.0 0.04351387324664687 0.0 20 0.0 0.0 0.0 0.04351387324664687 0.0 21 0.0 0.0 0.0 0.046073512849390805 0.0 22 0.0 0.0 0.0 0.053752431657622605 0.0 23 0.0 0.0 0.0 0.053752431657622605 0.0 24 0.0 0.0 0.0 0.053752431657622605 0.0 25 0.0 0.0 0.0 0.053752431657622605 0.0 26 0.0 0.0 0.0 0.06911026927408621 0.0 27 0.0 0.0 0.0 0.08190846728780588 0.0 28 0.0 0.0 0.0 0.10494522371250128 0.0 29 0.0 0.0 0.0 0.13310125934268455 0.0 30 0.0 0.0 0.0 0.16893621378109963 0.0 31 0.0 0.0 0.0 0.19965188901402683 0.0 32 0.0 0.0 0.0 0.2636428790826252 0.0 33 0.0 0.0 0.0 0.3250742295484796 0.0 34 0.0 0.0 0.0 0.41466161564451726 0.0 35 0.0 0.0 0.0 0.5682399918091533 0.0 36 0.0 0.0 0.0 0.7090201699600697 0.0 37 0.0 0.0 0.0 1.0110576430838538 0.0 38 0.0 0.0 0.0 1.3822053854817242 0.0 39 0.0 0.0 0.0 1.7866284427152657 0.0 40 0.0 0.0 0.0 2.311354561277772 0.0 41 0.0 0.0 0.0 3.0331729292515615 0.0 42 0.0 0.0 0.0 3.844578683321388 0.0 43 0.0 0.0 0.0 4.671342275007679 0.0 44 0.0 0.0 0.0 5.439234155830859 0.0 45 0.0 0.0 0.0 6.202006757448551 0.0 46 0.0 0.0 0.0 6.893109450189414 0.0 47 0.0 0.0 0.0 7.666120610218082 0.0 48 0.0 0.0 0.0 8.280434114876625 0.0 49 0.0 0.0 0.0 8.968977168014744 0.0 50 0.0 0.0 0.0 9.550015357837616 0.0 51 0.0 0.0 0.0 10.207842735742807 0.0 52 0.0 0.0 0.0 10.840073717620559 0.0 53 0.0 0.0 0.0 11.741066857786423 0.0 54 0.0 0.0 0.0 12.595986485102898 0.0 55 0.0 0.0 0.0 13.412511518378212 0.0 56 0.0 0.0 0.0 14.157366642776697 0.0 57 0.0 0.0 0.0 14.856148254325792 0.0 58 0.0 0.0 0.0 15.478140677792567 0.0 59 0.0 0.0 0.0 15.98750895873861 0.0 60 0.0 0.0 0.0 16.583904986177945 0.0 61 0.0 0.0 0.0 17.175181734411794 0.0 62 0.0 0.0 0.0 17.873963345960888 0.0 63 0.0 0.0 0.0 18.429405139756323 0.0 64 0.0 0.0 0.0 19.07955359885328 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTGTT 15 0.0022088003 70.00001 44 TAAGCCG 15 0.0022088003 70.00001 40 TAGCACC 15 0.0022088003 70.00001 36 GCAAACT 15 0.0022088003 70.00001 30 CACCATT 15 0.0022088003 70.00001 30 TCGTAAG 15 0.0022088003 70.00001 37 AGCCGTC 15 0.0022088003 70.00001 42 AAGCCGT 15 0.0022088003 70.00001 41 TTAGCAC 15 0.0022088003 70.00001 35 CTCGTAA 15 0.0022088003 70.00001 36 TTTACAC 130 0.0 59.230774 2 CTTTACA 135 0.0 57.037037 1 TCCACCA 25 2.3624496E-4 55.999996 28 AGGCGTC 20 0.0069021042 52.5 25 TAGCAAA 20 0.0069021042 52.5 28 ATCAGAG 20 0.0069021042 52.5 7 CTGCGTG 20 0.0069021042 52.5 9 TCTAATT 20 0.0069021042 52.5 55 TCTAACT 20 0.0069021042 52.5 63 CTAATTT 20 0.0069021042 52.5 56 >>END_MODULE