FastQCFastQC Report
Wed 25 May 2016
SRR1780449_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780449_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482547
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT207.83778E-444.016122
ATCGATA404.1027488E-738.5180923
AATCGAT451.0325348E-634.2347522
GCGCGTA353.245437E-431.37813833
GCGTACC300.005716828629.3593235
CGAGAAT406.9304137E-427.5644714
GTAATCG609.50008E-625.74828120
TAATCGA609.5154555E-625.74291621
CTATACA1400.025.1207431
CATCGTA450.001388962724.46864324
GCATATT450.001388962724.46864324
CGCGTAC450.001389804324.46609934
CGGATTC450.001393174624.4559323
ATCGTAC450.001404174724.42294525
AGCGCGT450.001409275824.4077532
GGGTCTA551.6012072E-423.978891
CGTTATC551.6125116E-423.9540378
ATCACTA500.002537233822.06076215
CGATCAA703.188302E-522.0194935
AACGTAA703.1959862E-522.01262510
Produced by FastQC (version 0.11.5)