FastQCFastQC Report
Wed 25 May 2016
SRR1780449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780449_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482547
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT365197.567967472598525No Hit
ATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCTT283585.87673325085432RNA PCR Primer, Index 17 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC81251.683773808561653No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG47000.9739983877218177No Hit
CTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC43870.909134239773535No Hit
TATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTCT27090.5613960919868946RNA PCR Primer, Index 17 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC19580.40576358365091897No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC16480.34152113680118207No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG12440.2577987221970088No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC9930.2057830636186734No Hit
CTTATAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG7840.1624712204199798No Hit
CTTAACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC7030.1456852907592421No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG6120.1268270241033516No Hit
TTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGTC6000.12434021970916821No Hit
CTTATTACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG5430.11252789883679724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGAA207.8530284E-443.99875625
ATACCAG207.8570377E-443.9941985
CCGTCTT29200.043.17001344
GCCGTCT32400.038.90630743
CTTTACA10500.036.2842031
CTATACA5750.036.001511
TTTACAC10650.035.736082
GTTATCA250.002349331735.20630313
GTACGTA250.002349331735.20630313
CTTATAA1200.034.8684841
TTACACA11400.033.5779953
CTTATAC54900.030.0047471
TTATACA55050.029.772092
TCTTTAT2000.029.729971
CAGTTAC300.005713399529.3629361
TACGTAG300.005736614629.33858314
GCAATTA300.005742429829.33250225
CGAGAAT407.0186175E-427.50492314
AGATACA407.0271286E-427.4992222
CTTTATA2250.027.3770072
Produced by FastQC (version 0.11.5)