FastQCFastQC Report
Wed 25 May 2016
SRR1780448_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780448_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87622
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGGC150.002219538269.98801416
TTTCTGG150.002219538269.98801413
TTCTGGG150.002219538269.98801414
TCTGGGG150.002219538269.98801415
ACGTTAT200.00661217153.127821
TCTTGCC200.00693541952.49101340
CTATCGT200.00693541952.4910133
CCTAACG200.00693541952.49101368
CGTTATC200.00693541952.4910132
GGCCACA200.00693541952.49101336
CCTCATG200.00693541952.49101348
CTTGCCA200.00693541952.49101341
GGGCCAC200.00693541952.49101335
AGTTATA200.00693541952.4910132
CATTTGT200.00693541952.49101322
TATCGTA200.00693541952.4910134
GTTATCG200.00693541952.4910133
TAGATCT552.9740817E-431.81273336
GCTATTG450.00429057131.10578519
GTAGATC450.00429057131.10578535
Produced by FastQC (version 0.11.5)