##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780448_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 87622 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.84753828947068 34.0 23.0 34.0 12.0 34.0 2 29.790417931569696 34.0 31.0 34.0 12.0 34.0 3 29.969927643742437 34.0 31.0 34.0 11.0 34.0 4 30.079774485859716 34.0 32.0 34.0 11.0 34.0 5 30.23046723425624 34.0 32.0 34.0 11.0 34.0 6 32.99639359978088 38.0 34.0 38.0 11.0 38.0 7 32.39187646937984 37.0 31.0 38.0 11.0 38.0 8 31.5624386569583 37.0 27.0 38.0 11.0 38.0 9 32.22463536554746 37.0 31.0 38.0 12.0 38.0 10-11 32.33411700257926 37.0 31.0 38.0 11.0 38.0 12-13 32.58473899249047 37.0 32.0 38.0 11.0 38.0 14-15 32.26492205153957 37.0 31.0 38.0 11.0 38.0 16-17 32.05846134532423 37.0 31.0 38.0 11.0 38.0 18-19 32.08667914450709 37.0 29.5 38.0 11.0 38.0 20-21 32.15715801967542 37.0 31.0 38.0 11.0 38.0 22-23 32.10271963662095 37.0 31.0 38.0 11.0 38.0 24-25 32.39315468717902 37.0 31.0 38.0 11.0 38.0 26-27 32.40193102188948 37.0 31.0 38.0 11.0 38.0 28-29 32.477009198603085 37.0 31.5 38.0 11.0 38.0 30-31 32.34002875990048 37.0 31.0 38.0 11.0 38.0 32-33 32.14816484444546 37.0 31.0 38.0 11.0 38.0 34-35 32.07046175617995 37.0 31.0 38.0 11.0 38.0 36-37 32.034078199538925 37.0 31.0 38.0 11.0 38.0 38-39 32.375950103855196 37.0 31.0 38.0 11.0 38.0 40-41 32.015447033849945 37.0 31.0 38.0 11.0 38.0 42-43 32.29008125813152 37.0 31.0 38.0 11.0 38.0 44-45 31.530722877816075 37.0 27.0 38.0 11.0 38.0 46-47 32.10908790029901 37.0 31.0 38.0 11.0 38.0 48-49 31.913497751706192 37.0 29.5 38.0 11.0 38.0 50-51 31.908852799525235 37.0 29.0 38.0 11.0 38.0 52-53 31.938588482344613 37.0 31.0 38.0 11.0 38.0 54-55 31.68093058820844 37.0 27.0 38.0 11.0 38.0 56-57 31.699304969071697 37.0 29.0 38.0 11.0 38.0 58-59 31.605007874734653 37.0 29.0 38.0 11.0 38.0 60-61 31.654048070119376 37.0 27.0 38.0 11.0 38.0 62-63 31.663086895985025 37.0 28.0 38.0 11.0 38.0 64-65 31.697136563876654 37.0 28.0 38.0 11.0 38.0 66-67 31.72057245897149 37.0 28.0 38.0 11.0 38.0 68-69 31.280808472757982 37.0 26.5 38.0 11.0 38.0 70-71 31.146082034192325 37.0 27.0 38.0 11.0 38.0 72-73 31.190540047020153 37.0 27.0 38.0 11.0 38.0 74-75 30.806715208509278 36.5 25.5 38.0 11.0 38.0 76 30.093686517084752 36.0 24.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.801468134711918 1101 2 0.058858598000814055 1101 3 0.1118382973126053 1101 4 -2.035388584998664 1101 5 -0.3986950069108275 1101 6 -0.45232530186096653 1101 7 0.1437190954323313 1101 8 0.10439870633070214 1101 9 -0.006799171036973917 1101 10-11 -0.29194930365024874 1101 12-13 -0.053488360690934655 1101 14-15 0.7130957426471269 1101 16-17 -1.5472169982896986 1101 18-19 -0.00526900774053729 1101 20-21 0.13060534914494326 1101 22-23 0.2843966164517866 1101 24-25 -0.33591293427323876 1101 26-27 -0.08733888447792992 1101 28-29 0.590381778892052 1101 30-31 -0.1510300283349295 1101 32-33 0.5427518237939992 1101 34-35 -0.11396311161215067 1101 36-37 0.8725118760308384 1101 38-39 0.28942698951799173 1101 40-41 1.0848712377508676 1101 42-43 -0.14682076714324666 1101 44-45 1.4560851613923695 1101 46-47 1.6922046422971313 1101 48-49 0.45664072289716273 1101 50-51 0.2922555397759581 1101 52-53 1.137067123214905 1101 54-55 -0.5469143723631511 1101 56-57 -0.1919909865158118 1101 58-59 0.599388646777971 1101 60-61 0.7749001879539144 1101 62-63 -0.7536941575678249 1101 64-65 0.3763317528582846 1101 66-67 0.03565680392764392 1101 68-69 0.8099684400006311 1101 70-71 1.3617783034850248 1101 72-73 0.5130920541174966 1101 74-75 -0.1050851941733022 1101 76 -1.2045073177797718 1102 1 0.6554589624623226 1102 2 -0.4751125243306582 1102 3 -0.23234630716963522 1102 4 0.3843120084994318 1102 5 -0.008049245550211026 1102 6 0.18694744151754605 1102 7 1.1698950188976482 1102 8 1.1401193193167956 1102 9 0.3380497969849898 1102 10-11 -0.2828143049789702 1102 12-13 -3.9171720350642154 1102 14-15 -1.2237454301759811 1102 16-17 -3.1234527144735935 1102 18-19 -0.9627608139235484 1102 20-21 -0.47661185344164636 1102 22-23 -1.2377266894211871 1102 24-25 -0.30517504783511384 1102 26-27 -4.288895709706026 1102 28-29 0.15429355141397139 1102 30-31 0.3567873159825439 1102 32-33 -6.2957746178748835 1102 34-35 -0.6099769303737794 1102 36-37 0.21853455297299362 1102 38-39 0.21428747317465024 1102 40-41 0.16043151766937314 1102 42-43 0.1225786482168374 1102 44-45 -5.867836355128137 1102 46-47 -0.462558844282718 1102 48-49 -0.013662226876956396 1102 50-51 -5.506586253119782 1102 52-53 -1.0255137076824283 1102 54-55 0.5809534635833451 1102 56-57 -1.5723280402849689 1102 58-59 0.5362408923267417 1102 60-61 0.16801516713718812 1102 62-63 -4.372515576648343 1102 64-65 -0.24142668455866811 1102 66-67 -0.1570529134964005 1102 68-69 -7.423721435099026 1102 70-71 -0.7187314848044579 1102 72-73 -0.31884611578240296 1102 74-75 -6.1186382103837715 1102 76 -1.8705539118093597 1103 1 0.3637425386107189 1103 2 0.5687534099102436 1103 3 0.6021811986021994 1103 4 1.3003218803249226 1103 5 0.9869352302080898 1103 6 -0.21443381793631744 1103 7 1.6113009579152475 1103 8 2.080151960030115 1103 9 0.978046612525155 1103 10-11 1.7207980666474896 1103 12-13 -0.03693757920878937 1103 14-15 0.60401509844435 1103 16-17 0.7687632715147821 1103 18-19 0.9154833545343806 1103 20-21 0.6285847869532333 1103 22-23 1.0842421028724232 1103 24-25 1.3375285585656442 1103 26-27 0.5308937178873165 1103 28-29 1.5389762996028153 1103 30-31 1.4427478286805808 1103 32-33 -3.0103176478884173 1103 34-35 0.6506711072028502 1103 36-37 0.9777296807494444 1103 38-39 1.292844116753983 1103 40-41 1.8656699540995945 1103 42-43 1.6603344001474127 1103 44-45 -3.0940723236019885 1103 46-47 0.5759034596739703 1103 48-49 0.6777437121406393 1103 50-51 1.2690785028709826 1103 52-53 1.2940766911711634 1103 54-55 0.8809693858825192 1103 56-57 1.6061310792118917 1103 58-59 1.5837590039420597 1103 60-61 2.3838916897070455 1103 62-63 1.7634509069118813 1103 64-65 1.8115846202737487 1103 66-67 1.3479228049973528 1103 68-69 -1.580267490349403 1103 70-71 1.1598112267630967 1103 72-73 1.9430558028546479 1103 74-75 -0.07361790945231661 1103 76 1.1534887599524453 1104 1 -1.611921449343832 1104 2 -0.7641597332870518 1104 3 -0.44384712921614167 1104 4 -0.8293417924870248 1104 5 -0.6554304402577955 1104 6 -0.5881054043291627 1104 7 0.13384240792137092 1104 8 0.3366080063910921 1104 9 -0.14797541244114143 1104 10-11 -0.2866603082671588 1104 12-13 -1.2015120491564417 1104 14-15 -0.9255382744568834 1104 16-17 -0.6591040884656074 1104 18-19 0.37096070803684 1104 20-21 0.410444828622083 1104 22-23 -0.2764998828761307 1104 24-25 -0.12205440272433776 1104 26-27 0.331504744376808 1104 28-29 -0.3199098463782448 1104 30-31 -0.333203289200263 1104 32-33 -1.0962815465525608 1104 34-35 -0.4175397582137528 1104 36-37 -0.29964833280168435 1104 38-39 -0.2726967905065507 1104 40-41 0.5992869157747478 1104 42-43 -0.10804454132360064 1104 44-45 -2.7459973196627026 1104 46-47 -0.4107992733127048 1104 48-49 0.38700715384801043 1104 50-51 0.584474969772252 1104 52-53 -0.35015357302338046 1104 54-55 -0.5304142652196013 1104 56-57 0.18842706814687915 1104 58-59 0.1443889389876638 1104 60-61 -0.3663052744192221 1104 62-63 0.03395706094658024 1104 64-65 -0.24601644420793178 1104 66-67 -0.6700490772793799 1104 68-69 -3.571014553395429 1104 70-71 -1.3543789442893086 1104 72-73 0.008465400697669878 1104 74-75 -0.874999284524538 1104 76 0.1799432306004114 1105 1 -1.2309883454069848 1105 2 -0.397800053092741 1105 3 -0.8844401865989973 1105 4 -0.3045973334241765 1105 5 -0.4594988543859202 1105 6 -0.043187548511596674 1105 7 -0.08850350848384636 1105 8 0.743010867100196 1105 9 0.4096098522081846 1105 10-11 -0.3545852821227058 1105 12-13 -1.3041754098149454 1105 14-15 -0.2009658627576627 1105 16-17 0.9054052392002809 1105 18-19 0.2960020331388833 1105 20-21 0.019188882867602786 1105 22-23 -0.27952757912819237 1105 24-25 -0.07185705427782096 1105 26-27 -0.43046266782844 1105 28-29 -0.8593204998208748 1105 30-31 -0.3489574301441607 1105 32-33 0.40182087223096374 1105 34-35 0.09787703495832645 1105 36-37 -0.05340132676316145 1105 38-39 -0.7159548952867709 1105 40-41 -0.5558593244843344 1105 42-43 0.8075763472504249 1105 44-45 0.9099669889430366 1105 46-47 0.21344591104785238 1105 48-49 -0.5017355510064263 1105 50-51 -0.14393477283139333 1105 52-53 -0.45473981598124524 1105 54-55 -0.8032695767602682 1105 56-57 -0.44264940859299173 1105 58-59 -0.06206789736492979 1105 60-61 -0.27279247207128066 1105 62-63 -0.012740304367319766 1105 64-65 -0.4393673196253971 1105 66-67 -0.38224082575288065 1105 68-69 0.24856342454202718 1105 70-71 0.21847130703478257 1105 72-73 -0.27251999214380973 1105 74-75 -0.8355042941389463 1105 76 -0.7749871070847085 1106 1 -0.9363882815024134 1106 2 0.16466666327747959 1106 3 -1.3882744607495994 1106 4 -1.0206480310490562 1106 5 0.20798437066466846 1106 6 -0.6506359810745295 1106 7 -0.0911661988662118 1106 8 0.7936374869035134 1106 9 1.0336912240262706 1106 10-11 -0.3552065765452568 1106 12-13 0.10203575928364472 1106 14-15 -0.03683762758885223 1106 16-17 -0.1865552109719033 1106 18-19 -0.2331633021825894 1106 20-21 0.06589247217424088 1106 22-23 -0.22196908869613097 1106 24-25 -0.1602051211646014 1106 26-27 -0.4293443378678461 1106 28-29 -0.8912284062361842 1106 30-31 -0.6685690323737177 1106 32-33 0.06799937899419461 1106 34-35 0.28550795056878897 1106 36-37 0.14015851276536395 1106 38-39 0.09018349360803768 1106 40-41 -0.17189937134768485 1106 42-43 -0.041156212127567926 1106 44-45 0.5946955720367306 1106 46-47 0.10905069679003887 1106 48-49 -0.11205968918230269 1106 50-51 -0.05852454099981941 1106 52-53 -0.3979801326638892 1106 54-55 -0.37978642271475493 1106 56-57 0.47003209813473745 1106 58-59 -0.8429817327041569 1106 60-61 -0.5916762722629976 1106 62-63 0.24212354339779552 1106 64-65 0.5550942843792868 1106 66-67 0.3464487755536112 1106 68-69 0.9854097340531567 1106 70-71 0.3032158662707687 1106 72-73 0.514988439709068 1106 74-75 -0.8060815654138445 1106 76 -0.9065595145118372 1107 1 -0.45347730258001917 1107 2 0.2016814141628771 1107 3 0.8388169672323862 1107 4 0.5589517351373772 1107 5 0.43007287597705 1107 6 0.42342790504451244 1107 7 -0.02368691543603063 1107 8 1.1221799343814212 1107 9 -0.5124181869698425 1107 10-11 -0.13801784085774216 1107 12-13 0.17934037022474314 1107 14-15 -0.39901656373711347 1107 16-17 0.6442823917513287 1107 18-19 0.8707891786481312 1107 20-21 0.7917546597064771 1107 22-23 -0.23609245973763393 1107 24-25 0.18899372352129973 1107 26-27 0.4734167252961967 1107 28-29 -0.3978903737085915 1107 30-31 0.1542914015567547 1107 32-33 0.9875228119275263 1107 34-35 0.7791554422305929 1107 36-37 0.2794838918528093 1107 38-39 0.7032564228106679 1107 40-41 0.7373517375475487 1107 42-43 0.06530632612501819 1107 44-45 0.1431946952358487 1107 46-47 0.28159135628183307 1107 48-49 0.30456686241141284 1107 50-51 1.040416421074248 1107 52-53 0.978219021481145 1107 54-55 0.7506162180031914 1107 56-57 1.1579683495530944 1107 58-59 1.1048228266604205 1107 60-61 0.21798916802858415 1107 62-63 -0.46906512352102325 1107 64-65 0.19151418604005244 1107 66-67 1.262164141918845 1107 68-69 1.2990723548281764 1107 70-71 0.573554208257498 1107 72-73 0.5631179311645589 1107 74-75 -0.11176701739610095 1107 76 0.15485093145315787 1108 1 -0.36038930468854247 1108 2 -0.12420070622060919 1108 3 0.12499955214985548 1108 4 -0.11651691705515788 1108 5 0.21118182731629176 1108 6 0.22268992197858495 1108 7 0.9835446596965767 1108 8 1.2805827281744584 1108 9 -0.6085042410666368 1108 10-11 0.20657742155453462 1108 12-13 0.48259704995986397 1108 14-15 0.5807465568769956 1108 16-17 -0.9940317781824426 1108 18-19 -0.15926551116835697 1108 20-21 0.15981547741807134 1108 22-23 -0.05056714098824955 1108 24-25 -0.7602946499508505 1108 26-27 -0.38239653863158196 1108 28-29 -0.04887215475164908 1108 30-31 0.48876410606701626 1108 32-33 0.6116126877223351 1108 34-35 0.2710919888651908 1108 36-37 0.7416457868878972 1108 38-39 0.637414496147727 1108 40-41 0.4928701377056868 1108 42-43 0.5605868585270954 1108 44-45 0.3429278352879024 1108 46-47 0.5941140064317416 1108 48-49 0.27383678630690156 1108 50-51 0.2701465960157634 1108 52-53 0.35734134876345536 1108 54-55 0.6610039885695294 1108 56-57 0.8369539522941736 1108 58-59 -1.1911116773424801 1108 60-61 0.683478575994851 1108 62-63 0.20004501386243234 1108 64-65 -0.0459088034502777 1108 66-67 -0.0743182486191607 1108 68-69 1.0049416263993578 1108 70-71 1.3357111614416937 1108 72-73 0.26264516076294697 1108 74-75 1.6447232801033245 1108 76 1.1103413277895982 1109 1 -0.7516453887121095 1109 2 -0.8372226514003565 1109 3 0.18101526387387779 1109 4 0.06563661381325403 1109 5 0.6415464417813261 1109 6 0.7892000406704156 1109 7 -0.0423113730931064 1109 8 0.3602001818504874 1109 9 0.9341992001424799 1109 10-11 0.657145263754181 1109 12-13 0.7698995860370488 1109 14-15 0.2887305035937544 1109 16-17 -0.6770819019976635 1109 18-19 -0.13945507653425437 1109 20-21 0.7749380117874622 1109 22-23 0.811272155399763 1109 24-25 0.9088796306400511 1109 26-27 1.079442757756432 1109 28-29 -0.0507507304497139 1109 30-31 0.5284276702374484 1109 32-33 0.8511737841274325 1109 34-35 0.2981093229954901 1109 36-37 0.26060232347742485 1109 38-39 0.9108411890006067 1109 40-41 0.1273163848237786 1109 42-43 0.5180541969268901 1109 44-45 0.45601809231121493 1109 46-47 -0.4163035497120582 1109 48-49 0.4078048505200371 1109 50-51 1.1650829806114444 1109 52-53 0.9858359265108732 1109 54-55 0.39816563146936446 1109 56-57 0.9394644125452203 1109 58-59 0.409161570031781 1109 60-61 1.022279703140267 1109 62-63 0.11807934056382408 1109 64-65 0.2941817097424497 1109 66-67 0.567667918232516 1109 68-69 1.7460995121478007 1109 70-71 0.6523024004477556 1109 72-73 0.2941881545294933 1109 74-75 1.184480773058663 1109 76 0.45859510628844546 1110 1 -0.1318771424305467 1110 2 -0.04434821348959872 1110 3 -0.5439069083848587 1110 4 -0.4271686438830429 1110 5 -0.07576590688695362 1110 6 0.09388700019999163 1110 7 -0.651339391079965 1110 8 0.8409507871773627 1110 9 -1.7715600527112016 1110 10-11 -1.4595012253499355 1110 12-13 -0.6495832918598694 1110 14-15 -0.3969146019060723 1110 16-17 -0.6119400825580179 1110 18-19 -0.6144637240610606 1110 20-21 -1.071551092328754 1110 22-23 -0.9131744028845716 1110 24-25 -0.6150567235626525 1110 26-27 -1.738647868324513 1110 28-29 -0.5923885720270228 1110 30-31 -0.8676290575384016 1110 32-33 -0.07099847460657571 1110 34-35 -0.5416606527914318 1110 36-37 -0.6156515679096408 1110 38-39 -0.4123514778966637 1110 40-41 -0.9953487829382439 1110 42-43 -0.3505985183965308 1110 44-45 0.35626022306182037 1110 46-47 -0.6674277281970156 1110 48-49 -0.8984559588884693 1110 50-51 -0.4994708070052951 1110 52-53 -0.7474916418045865 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2116 38-39 0.2041108372854481 2116 40-41 -0.12965278582320394 2116 42-43 0.20007764460383015 2116 44-45 0.974255174378083 2116 46-47 -0.23623407511371042 2116 48-49 -0.10083605264973983 2116 50-51 -0.1873269195188172 2116 52-53 0.6637777415347514 2116 54-55 0.19191291761786644 2116 56-57 -0.4520249491569075 2116 58-59 -1.1239257074323952 2116 60-61 -1.2782413563473582 2116 62-63 0.3449573258004719 2116 64-65 1.073035378869669 2116 66-67 -0.5822235277075585 2116 68-69 -0.20096952823431025 2116 70-71 -2.3829125365908865 2116 72-73 0.2520691792798182 2116 74-75 -0.7743989490429435 2116 76 -0.4834804473372607 2117 1 0.04981485055601098 2117 2 -0.3125393297958503 2117 3 0.3763882406507939 2117 4 0.23808559340509206 2117 5 -0.6552515877753251 2117 6 -1.504008967070611 2117 7 -1.8969559895603858 2117 8 -2.903716074307148 2117 9 -0.5027114261770116 2117 10-11 -0.6488276426497599 2117 12-13 -0.39220456596857645 2117 14-15 -0.6722652740276374 2117 16-17 -0.3184348224761955 2117 18-19 -0.7088164417700042 2117 20-21 -0.5596063231879036 2117 22-23 -0.13537633158123796 2117 24-25 -0.35234485915587044 2117 26-27 0.4336563397409421 2117 28-29 -0.4330519267903199 2117 30-31 -0.0884794374332003 2117 32-33 0.5495815955278474 2117 34-35 -0.5861887065611562 2117 36-37 -1.2299852908786626 2117 38-39 -0.44478864914155025 2117 40-41 -1.2873563808342112 2117 42-43 -0.6388071683088654 2117 44-45 -0.19151885350159503 2117 46-47 -0.825522409669837 2117 48-49 -0.9339615111970616 2117 50-51 0.03895701298301546 2117 52-53 0.22489292862206156 2117 54-55 -1.39627490336526 2117 56-57 -2.1424321391789185 2117 58-59 -0.9033736164566051 2117 60-61 0.214697014892554 2117 62-63 -0.6820877952560203 2117 64-65 -0.49724158371286364 2117 66-67 -1.422383102175644 2117 68-69 0.03110127147221675 2117 70-71 0.5268361794766108 2117 72-73 0.7525185556554632 2117 74-75 -0.6305526541053048 2117 76 -0.6621121421281622 2118 1 -0.13722585040498458 2118 2 -0.0056569103498382844 2118 3 -0.11364850327232645 2118 4 0.4552987081591908 2118 5 -0.01887510388612057 2118 6 -0.15847476695755347 2118 7 0.5493413535713287 2118 8 -4.177387640163566 2118 9 -0.7869115392352377 2118 10-11 -0.6916837760584684 2118 12-13 0.30769650808456106 2118 14-15 -0.9081329959044027 2118 16-17 -0.22540910173001194 2118 18-19 -0.6092192116965123 2118 20-21 -2.807380489383494 2118 22-23 -0.40046253847778956 2118 24-25 0.14765514084412956 2118 26-27 0.8271342933870756 2118 28-29 -0.18637301214927504 2118 30-31 -0.5992906301974728 2118 32-33 0.19765253956402518 2118 34-35 0.9774418756887009 2118 36-37 -0.2937218652144473 2118 38-39 0.17187330676574675 2118 40-41 -0.8355474492344328 2118 42-43 -0.5484312497107879 2118 44-45 0.45941387685453705 2118 46-47 -0.25818210441262934 2118 48-49 -0.996920810236066 2118 50-51 -0.43115321878634205 2118 52-53 -1.9818623003208415 2118 54-55 -0.027846412692561273 2118 56-57 0.7570873633648887 2118 58-59 0.7858929183088001 2118 60-61 -2.2499016284087467 2118 62-63 -0.17625823053019118 2118 64-65 -0.20601066228833176 2118 66-67 -1.7676699874215487 2118 68-69 1.1288613053896839 2118 70-71 -0.12752362832440767 2118 72-73 -2.1283110090703694 2118 74-75 0.15135096375789558 2118 76 -0.6993506949828827 2119 1 1.2437803677973918 2119 2 0.6529779115834593 2119 3 0.1565606544438971 2119 4 0.9406718003016437 2119 5 0.5128518605005361 2119 6 1.3882324122397307 2119 7 -0.7374732309396954 2119 8 1.6092149601756134 2119 9 -0.06736659859080874 2119 10-11 0.5429827021778308 2119 12-13 0.844864399548662 2119 14-15 0.273855415627537 2119 16-17 0.35397897062724937 2119 18-19 -0.15493713142517862 2119 20-21 -0.818227012843078 2119 22-23 0.789192633936004 2119 24-25 1.0549827270510264 2119 26-27 0.5478804776719812 2119 28-29 0.7587584180372673 2119 30-31 0.5386757349805933 2119 32-33 0.32759883690716407 2119 34-35 0.9008802589560823 2119 36-37 0.3626871229144406 2119 38-39 -0.2637541663829239 2119 40-41 0.1113505157175183 2119 42-43 -0.1086347545157551 2119 44-45 -0.46738092246711105 2119 46-47 0.6593913834336114 2119 48-49 0.5186246956994864 2119 50-51 1.5303363233278446 2119 52-53 -0.3699346575848317 2119 54-55 -0.8380852481928436 2119 56-57 1.2045506194417754 2119 58-59 0.8530919007847722 2119 60-61 0.47331770454772837 2119 62-63 0.23170530819225377 2119 64-65 0.9531894507698908 2119 66-67 0.7026168978243561 2119 68-69 0.24230816802394628 2119 70-71 0.83287066223523 2119 72-73 -0.44575730641350475 2119 74-75 0.5181542424464176 2119 76 0.9025430302856314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 62.0 13 345.0 14 804.0 15 1183.0 16 1694.0 17 2093.0 18 2415.0 19 2290.0 20 2015.0 21 1679.0 22 1325.0 23 1073.0 24 922.0 25 948.0 26 1002.0 27 1156.0 28 1406.0 29 1722.0 30 1967.0 31 2540.0 32 3156.0 33 4124.0 34 5953.0 35 8926.0 36 15427.0 37 21392.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.917631237140654 26.962378877918137 10.515063391533236 28.604926493407973 2 16.878181278674305 26.78893428590993 32.292118417748966 24.040766017666794 3 15.710666271027824 26.923603661180984 28.178996142521285 29.18673392526991 4 12.892880783364907 21.99561753897423 35.64629887471183 29.46520280294903 5 10.873981420191276 38.422998790258156 33.42311291684737 17.2799068727032 6 22.14398210495081 41.36746479194723 17.985209194038028 18.503343909063933 7 22.24555476935016 33.46876355253247 22.847001894501382 21.43867978361599 8 19.708520691150625 36.18155257811965 20.895437218963274 23.214489511766452 9 20.425235671406725 20.58957795987309 21.322270662618976 37.66291570610121 10-11 21.310287370751638 27.552441167743265 27.20720823537468 23.930063226130425 12-13 21.232681290086962 25.40229622697496 25.983200566067882 27.38182191687019 14-15 19.556732327497663 26.907625938691197 23.068978110520188 30.466663623290952 16-17 20.635799228504258 28.853484284768665 24.579443518751 25.931272967976078 18-19 19.49795713405309 28.16358905297756 25.385177238593048 26.9532765743763 20-21 19.380977380110018 28.530506037296572 25.1905914039853 26.897925178608112 22-23 20.80698911232339 27.976421446668642 25.326972678094545 25.889616762913427 24-25 19.855173358289015 29.324256465271283 25.218552418342426 25.602017758097283 26-27 18.999794572139418 29.206135445436075 26.17835703362169 25.61571294880281 28-29 20.12850653945356 27.997534865673007 27.572983953801554 24.300974641071875 30-31 20.156467553810685 28.00723562575609 26.999497843007465 24.83679897742576 32-33 19.240031042432264 29.54337951655977 26.132135764990526 25.08445367601744 34-35 20.16616831389377 28.319942480199035 26.42315856748305 25.09073063842414 36-37 20.29741389148844 27.828056880692063 26.638287188149096 25.236242039670405 38-39 19.941909566090708 27.542169774714115 27.881696377622056 24.634224281573122 40-41 19.6080892926434 27.186665449316383 27.517061925087305 25.688183332952914 42-43 18.805779370477733 26.965830499189703 27.99981739745726 26.228572732875303 44-45 18.53301682225925 27.27226039122595 28.26402045148479 25.930702335030016 46-47 18.95300267056219 27.304786469151583 27.994681700942685 25.747529159343546 48-49 18.554130241263607 27.864577389240146 27.666567756956017 25.91472461254023 50-51 18.352126178357032 27.706512063180476 27.23573988267787 26.70562187578462 52-53 17.84198032457602 27.614640158864212 27.4794001506471 27.063979365912672 54-55 17.592043094200086 27.850882198534617 27.23973431330031 27.317340393964983 56-57 17.86366437652644 27.74531510351282 27.238593047408184 27.152427472552553 58-59 18.224304398438747 27.695099404259203 26.819748464997375 27.260847732304672 60-61 17.799855058404614 27.28268746825836 26.87867703702858 28.038780436308443 62-63 17.710734746981352 27.618634589486657 26.760402638606745 27.91022802492525 64-65 18.42117276483075 28.260596653808406 25.558079021250368 27.760151560110476 66-67 18.009175777772708 28.776448837050054 24.982881011618087 28.23149437355915 68-69 18.366392002008627 28.531076670242633 25.045650635685103 28.056880692063636 70-71 18.93474241628815 28.054598160279383 25.04679190157723 27.96386752185524 72-73 19.040309511309943 27.319622925749243 25.103284563237544 28.53678299970327 74-75 19.312501426582365 27.262559631142864 25.343521033530394 28.081417908744378 76 19.866015384264227 26.016297276939582 25.62826687361622 28.489420465179975 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.0 12 1.0 13 4.5 14 6.5 15 7.0 16 10.0 17 11.0 18 13.5 19 21.0 20 38.0 21 50.0 22 43.5 23 66.0 24 103.0 25 111.0 26 184.0 27 316.0 28 442.5 29 510.0 30 608.5 31 792.5 32 1097.5 33 1317.0 34 1616.5 35 2071.5 36 2488.0 37 2749.0 38 3048.5 39 3879.5 40 4833.0 41 5438.5 42 5622.0 43 5752.5 44 6224.5 45 6501.5 46 6437.0 47 6413.0 48 6074.5 49 5559.0 50 5358.0 51 5001.0 52 4207.0 53 3449.5 54 3129.0 55 2916.0 56 2347.0 57 1801.5 58 1612.0 59 1321.0 60 865.0 61 610.5 62 521.0 63 431.0 64 289.5 65 209.5 66 141.5 67 102.0 68 91.5 69 70.5 70 43.0 71 26.0 72 26.0 73 26.0 74 20.0 75 14.0 76 9.5 77 9.5 78 11.5 79 9.0 80 5.5 81 4.5 82 6.5 83 6.0 84 5.5 85 4.5 86 4.0 87 4.0 88 5.5 89 6.0 90 3.5 91 2.5 92 3.0 93 1.5 94 1.5 95 2.5 96 2.0 97 1.0 98 1.5 99 6.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7110086507954624 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 5.706329460637739E-4 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 87622.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.18782954052635 #Duplication Level Percentage of deduplicated Percentage of total 1 79.30204797254449 48.523201935586954 2 8.637669265490358 10.570404692885349 3 4.276867982243444 7.850768071945402 4 2.3837057484985267 5.834151240556025 5 1.4492483306599022 4.433817990915523 6 0.9922781363076808 3.6429207276711324 7 0.6957138060954229 2.9798452443450274 8 0.5315775730219718 2.6020862340508093 9 0.3581154176148021 1.9721074615964027 >10 1.372775767523408 11.590696400447376 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0034237976763826436 0.0 2 0.0 0.0 0.0 0.0045650635685101915 0.0 3 0.0 0.0 0.0 0.0045650635685101915 0.0 4 0.0 0.0 0.0 0.007988861244892835 0.0 5 0.0 0.0 0.0 0.007988861244892835 0.0 6 0.0 0.0 0.0 0.007988861244892835 0.0 7 0.0 0.0 0.0 0.007988861244892835 0.0 8 0.0 0.0 0.0 0.009130127137020383 0.0 9 0.0 0.0 0.0 0.013695190705530574 0.0 10 0.0 0.0 0.0 0.014836456597658122 0.0 11 0.0 0.0 0.0 0.018260254274040766 0.0 12 0.0 0.0 0.0 0.02054278605829586 0.0 13 0.0 0.0 0.0 0.02168405195042341 0.0 14 0.0 0.0 0.0 0.02168405195042341 0.0 15 0.0 0.0 0.0 0.023966583734678505 0.0 16 0.0 0.0 0.0 0.02739038141106115 0.0 17 0.0 0.0 0.0 0.03880304033233663 0.0 18 0.0 0.0 0.0 0.04336810390084682 0.0 19 0.0 0.0 0.0 0.04793316746935701 0.0 20 0.0 0.0 0.0 0.050215699253612106 0.0 21 0.0 0.0 0.0 0.0547807628221223 0.0 22 0.0 0.0 0.0 0.05934582639063249 0.0 23 0.0 0.0 0.0 0.061628358174887585 0.0 24 0.0 0.0 0.0 0.061628358174887585 0.0 25 0.0 0.0 0.0 0.06619342174339778 0.0 26 0.0 0.0 0.0 0.07075848531190797 0.0 27 0.0 0.0 0.0 0.09472506904658648 0.0 28 0.0 0.0 0.0 0.11412658921275479 0.0 29 0.0 0.0 0.0 0.12325671634977517 0.0 30 0.0 0.0 0.0 0.15635342722147405 0.0 31 0.0 0.0 0.0 0.1860263404167903 0.0 32 0.0 0.0 0.0 0.22368811485699938 0.0 33 0.0 0.0 0.0 0.2670562187578462 0.0 34 0.0 0.0 0.0 0.333249640501244 0.0 35 0.0 0.0 0.0 0.40629065759740707 0.0 36 0.0 0.0 0.0 0.5363949692999475 0.0 37 0.0 0.0 0.0 0.7669306795097122 0.0 38 0.0 0.0 0.0 1.0088790486407524 0.0 39 0.0 0.0 0.0 1.3364223596813585 0.0 40 0.0 0.0 0.0 1.7210289653283422 0.0 41 0.0 0.0 0.0 2.1490036748761727 0.0 42 0.0 0.0 0.0 2.635182944922508 0.0 43 0.0 0.0 0.0 3.068863983930976 0.0 44 0.0 0.0 0.0 3.561890849330077 0.0 45 0.0 0.0 0.0 4.041222524023647 0.0 46 0.0 0.0 0.0 4.518271666932962 0.0 47 0.0 0.0 0.0 5.076350688183333 0.0 48 0.0 0.0 0.0 5.616169455159663 0.0 49 0.0 0.0 0.0 6.114902650019402 0.0 50 0.0 0.0 0.0 6.623907237908288 0.0 51 0.0 0.0 0.0 7.186551322727169 0.0 52 0.0 0.0 0.0 7.752619205222433 0.0 53 0.0 0.0 0.0 8.230809614023876 0.0 54 0.0 0.0 0.0 8.747803063157654 0.0 55 0.0 0.0 0.0 9.24311246034101 0.0 56 0.0 0.0 0.0 9.733856793955855 0.0 57 0.0 0.0 0.0 10.245143913628997 0.0 58 0.0 0.0 0.0 10.710780397617036 0.0 59 0.0 0.0 0.0 11.152450297870399 0.0 60 0.0 0.0 0.0 11.587272602770994 0.0 61 0.0 0.0 0.0 12.047202757298395 0.0 62 0.0 0.0 0.0 12.520828102531327 0.0 63 0.0 0.0 0.0 13.009289904361918 0.0 64 0.0 0.0 0.0 13.42242815731209 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACTGGC 15 0.0022195382 69.988014 16 TTTCTGG 15 0.0022195382 69.988014 13 TTCTGGG 15 0.0022195382 69.988014 14 TCTGGGG 15 0.0022195382 69.988014 15 ACGTTAT 20 0.006612171 53.12782 1 TCTTGCC 20 0.006935419 52.491013 40 CTATCGT 20 0.006935419 52.491013 3 CCTAACG 20 0.006935419 52.491013 68 CGTTATC 20 0.006935419 52.491013 2 GGCCACA 20 0.006935419 52.491013 36 CCTCATG 20 0.006935419 52.491013 48 CTTGCCA 20 0.006935419 52.491013 41 GGGCCAC 20 0.006935419 52.491013 35 AGTTATA 20 0.006935419 52.491013 2 CATTTGT 20 0.006935419 52.491013 22 TATCGTA 20 0.006935419 52.491013 4 GTTATCG 20 0.006935419 52.491013 3 TAGATCT 55 2.9740817E-4 31.812733 36 GCTATTG 45 0.004290571 31.105785 19 GTAGATC 45 0.004290571 31.105785 35 >>END_MODULE