FastQCFastQC Report
Wed 25 May 2016
SRR1780448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780448_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87622
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGT38604.405286343612335No Hit
ATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCTT34463.93280226427153No Hit
CTTTACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC10661.216589441007966No Hit
CTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC5280.6025883910433453No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG3550.40514939170527947No Hit
TATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTCT3200.3652050854808153No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC1970.22482938074912692No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC1840.2099929241514688No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG1780.2031453287987035No Hit
CTTATACACATCTCCGAGCCCACGAGACAATTCGTTATATCGTATGCCGT1420.16205975668211178No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCC1340.15292962954509143No Hit
CTTATAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCG1300.14836456597658124No Hit
CTTAACACATCTCCGAGCCCACGAGACAATTCGTTATCTCGTATGCCGTC1020.11640912099700987No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC950.10842025975211704No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATT207.8878664E-570.066
CGGATTC150.002218031370.067
CTCGGAT150.002218031370.065
TCGCTAA150.002218031370.014
GCTTGAC308.315919E-658.33333253
CTTGACA308.315919E-658.33333254
CTATGCA252.3788222E-455.99999638
GATCCTA252.3788222E-455.9999961
TTTACAC1650.053.0303082
TCGTTCT200.00693072552.532
AGGACGC200.00693072552.556
ACACAAG200.00693072552.518
ACTTCGT200.00693072552.529
AAGCGAG200.00693072552.558
ATACTCG200.00693072552.562
AGACACT200.00693072552.525
GGCATTA200.00693072552.564
ACACTTC200.00693072552.527
CCAGTAA200.00693072552.526
TTATTGG200.00693072552.551
Produced by FastQC (version 0.11.5)