FastQCFastQC Report
Wed 25 May 2016
SRR1780447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599133
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGACCAAGCCCGTGTAGATCTCGGTGGTC46300.7727833385909305No Hit
ATACACATCTGACGCTGCCGACGACCAAGCCCGTGTAGATCTCGGTGGGC18520.3091133354363722No Hit
CTTTACACATCTGACGCTGCCGACGACCAAGCCCGTGTAGATCTCGGTGG11350.18944040805630802No Hit
CTATACACATCTGACGCTGCCGACGACCAAGCCCGTGTAGATCTCGGTGG9660.16123298165849653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA357.2011917E-637.77743516
CGGTGGT7550.037.2544243
TTAATAC401.8362993E-532.953182
GGTGGTC7950.032.33951644
TCGGTGG13750.029.41295642
TGACGGT454.0499435E-529.35050613
TACATGC752.1955202E-929.2917122
CTATACA3350.027.5890081
ACGGTGC406.9595466E-427.5460515
CTTATAA407.0176553E-427.5068971
TCCGATC407.08653E-427.4609813
GTACATG905.11136E-1026.8956341
TTTACAC4500.026.8507352
CTCGGTG14750.026.82279641
CCGTGTA15300.026.0087531
CCCGTGT16050.025.3497830
ACGAGAG701.1110496E-625.19339621
GACGGTG701.1175562E-625.17652714
ACCAAGC17300.024.9519724
TCTCGGT15800.024.93238640
Produced by FastQC (version 0.11.5)