FastQCFastQC Report
Wed 25 May 2016
SRR1780447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences599133
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT308685.152111467737548No Hit
ATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCTT235453.929845293115218RNA PCR Primer, Index 43 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC66831.1154451515773627No Hit
CTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC37780.6305778516623187No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG34210.5709917497450483No Hit
TATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTCT20340.3394905638647846RNA PCR Primer, Index 43 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC19250.32129760837743876No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC13980.233337172213849No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG10750.1794259371458424No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC8270.13803279071591784No Hit
CTTATAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG6690.1116613506516917No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC6340.1058195759539201No Hit
TTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC6210.1036497739233192No Hit
CTTAACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGTC6180.10314905037779591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAA650.044.038431
GGACCTA207.819764E-444.038431
TACGCTG207.858451E-443.99432434
CTGCTTG14150.042.2878324
CTTGAAA14700.041.45389627
TTGAAAA14900.041.3369528
TCTTCTG14700.040.4029520
CGTCTTC14750.040.2659918
GTCTTCT15350.038.8353819
TCTGCTT15650.038.7969523
GCTTGAA15750.038.4109526
CTTCTGC15900.038.32530221
TTCTGCT16400.037.15684522
TGCTTGA16250.037.09369725
TGAAAAA16700.036.8814729
GAGTCGG250.002352374435.19839523
AGTCGGG250.002352374435.19839524
CTTGCGT250.002353337835.19545734
GAAAAAA17600.034.99548730
TTTACAC9750.034.9727632
Produced by FastQC (version 0.11.5)