FastQCFastQC Report
Wed 25 May 2016
SRR1780446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52517
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGGC150.002213780869.99428618
GCCCACG200.006917557752.49571619
GGACTCA200.006917557752.4957166
CTCGGTC200.006917557752.49571643
CTCTGGG200.006917557752.49571654
TTTGCCA200.006917557752.49571629
GCCCGTG200.006917557752.4957167
TTTACAC552.9564032E-431.8155862
TGCCCAC450.0042731831.10857218
CTATACA604.76011E-429.3318981
TTACACA604.922911E-429.1642863
TAATACA500.00715169227.9977152
CGTTGCT850.003745266520.58655570
ACGTTGC900.00521385919.44285869
AATACAC900.00521385919.4428583
GACGTTG1000.00957586517.49857168
ACGACGT1755.4626988E-515.99869370
GACGACG2052.102735E-413.65742169
CGACGAC2405.373192E-513.12392868
TGCCGAC4351.3239822E-59.65438470
Produced by FastQC (version 0.11.5)