FastQCFastQC Report
Wed 25 May 2016
SRR1780446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52517
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT38877.401412875830683No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT35366.733057866976408Illumina PCR Primer Index 2 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC11872.2602205000285625No Hit
CTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC5451.03775920178228No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG3900.7426166765047508No Hit
TATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT3260.6207513757449968No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1720.3275129957918388No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1590.3027591065750138No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG1550.29514252527752916No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGT1320.2513471828169926No Hit
CTTATAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG1180.22468914827579642No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC1010.19231867776148676No Hit
CTTAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC990.18851038711274445No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG760.14471504465220786No Hit
TTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC760.14471504465220786No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGT700.13329017270598092No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGTCTT640.12186530075975399No Hit
ATACACATCTCCGAGCCCACGAGACTTCCCTATATCTCGTATGCCGTCTT620.11805701011101168Illumina PCR Primer Index 6 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG610.11615286478664053No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGTCTT610.11615286478664053No Hit
ATACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGTCTT580.11044042881352704No Hit
CTTATACACATCTCCGAGCCCACGAGCCTTCCATATATCTCGTATGCCGT580.11044042881352704No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTGG150.00221306470.000011
AGACTCT150.00221306470.000016
CTATACA1050.066.666671
TTTACAC1450.055.5172422
TTACACA1500.053.6666683
TACTAAT200.006915325352.54
AGCTCGT200.006915325352.536
AACTAAA200.006915325352.570
CATCAGG200.006915325352.526
TAGCTCG200.006915325352.535
CTTTACA1550.051.9354861
ATAACAC352.0601143E-550.04
TATAACA352.0601143E-550.03
TTAATAC305.8280025E-446.6666682
ATATCGT305.8280025E-446.66666837
GAGCCTT305.8280025E-446.66666824
TTATAAC404.5348737E-543.752
CTTATAA404.5348737E-543.751
TAATACA350.001244856240.03
ACTTCCC350.001244856240.027
Produced by FastQC (version 0.11.5)