##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780446_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 52517 Sequences flagged as poor quality 0 Sequence length 76 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.80499647733115 34.0 34.0 34.0 31.0 34.0 2 33.28642153969191 34.0 34.0 34.0 32.0 34.0 3 33.48633775729764 34.0 34.0 34.0 34.0 34.0 4 33.47405601995544 34.0 34.0 34.0 34.0 34.0 5 33.51649941923568 34.0 34.0 34.0 34.0 34.0 6 37.27016013862178 38.0 37.0 38.0 36.0 38.0 7 36.84947731210846 38.0 37.0 38.0 35.0 38.0 8 36.93179351448102 38.0 37.0 38.0 35.0 38.0 9 36.70773273416227 38.0 37.0 38.0 34.0 38.0 10-11 36.44591275206124 38.0 37.0 38.0 34.0 38.0 12-13 36.289477692937524 38.0 37.0 38.0 34.0 38.0 14-15 36.435278100424625 38.0 37.0 38.0 34.0 38.0 16-17 36.51958413466116 38.0 37.0 38.0 34.0 38.0 18-19 36.59399813393758 38.0 37.0 38.0 34.0 38.0 20-21 36.34809680674829 38.0 37.0 38.0 34.0 38.0 22-23 36.366890721099836 38.0 37.0 38.0 33.5 38.0 24-25 35.435439952777195 38.0 35.5 38.0 29.0 38.0 26-27 35.6065083687187 38.0 36.0 38.0 31.0 38.0 28-29 35.568263609878706 38.0 36.0 38.0 31.0 38.0 30-31 35.321610145286286 38.0 36.0 38.0 29.0 38.0 32-33 35.83156882533275 38.0 36.5 38.0 32.0 38.0 34-35 35.62795856579774 38.0 36.0 38.0 31.0 38.0 36-37 35.60120532399033 38.0 36.0 38.0 31.0 38.0 38-39 35.06386503417941 37.5 35.5 38.0 29.0 38.0 40-41 34.972418454976484 37.0 35.5 38.0 29.0 38.0 42-43 35.58936154007274 37.5 36.0 38.0 32.0 38.0 44-45 35.80779557095798 38.0 36.5 38.0 31.5 38.0 46-47 35.9980387303159 38.0 37.0 38.0 32.5 38.0 48-49 35.69986861397262 38.0 36.5 38.0 31.5 38.0 50-51 35.8039301559495 38.0 36.5 38.0 31.0 38.0 52-53 35.69200449378296 38.0 36.5 38.0 31.0 38.0 54-55 35.918140792505284 38.0 37.0 38.0 33.0 38.0 56-57 35.841546546832454 38.0 37.0 38.0 31.5 38.0 58-59 35.861958984709716 38.0 37.0 38.0 32.0 38.0 60-61 35.67417217282023 38.0 36.5 38.0 32.0 38.0 62-63 36.0951406211322 38.0 37.0 38.0 33.5 38.0 64-65 36.047165679684674 38.0 37.0 38.0 33.0 38.0 66-67 35.00766418493059 38.0 35.5 38.0 29.0 38.0 68-69 33.23075385113392 37.0 32.5 38.0 21.5 38.0 70-71 32.01127254032028 37.0 28.5 38.0 16.0 38.0 72-73 30.750147571262637 37.0 24.5 38.0 11.0 38.0 74-75 30.977988080050267 37.0 25.0 38.0 11.0 38.0 76 30.707237656377934 37.0 25.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07782554171987499 1101 2 0.38391283987505886 1101 3 0.1599547552666465 1101 4 0.28188705338953923 1101 5 0.3700595891231373 1101 6 -0.3074185239087086 1101 7 0.35985541963957957 1101 8 0.5675644105832234 1101 9 0.6031477271010175 1101 10-11 0.6324053991742957 1101 12-13 0.5956574706155351 1101 14-15 0.5779459140268202 1101 16-17 -0.45052781338947767 1101 18-19 -0.4396251365734969 1101 20-21 -0.2439074781828836 1101 22-23 0.18554159340733634 1101 24-25 -2.292205079032108 1101 26-27 -0.7616137052701504 1101 28-29 1.0408912447066996 1101 30-31 1.1892149908155716 1101 32-33 0.11018741986718084 1101 34-35 0.41638495399665487 1101 36-37 -0.10275321841308482 1101 38-39 -0.1455192997743353 1101 40-41 0.6758725670356185 1101 42-43 0.7591449416726732 1101 44-45 0.37136050627191963 1101 46-47 0.8670638715327357 1101 48-49 -1.3379296914523167 1101 50-51 -1.63075540650496 1101 52-53 -2.4706619232860803 1101 54-55 -1.0878301552406526 1101 56-57 -1.5972567318763993 1101 58-59 -1.751790912180148 1101 60-61 -2.044174478110783 1101 62-63 -1.110085678723351 1101 64-65 -2.4242545842249115 1101 66-67 -2.2596909959653146 1101 68-69 -1.9029295992281234 1101 70-71 -1.9734949232811871 1101 72-73 -2.934669428046508 1101 74-75 -1.9744381320330682 1101 76 -2.7451860850513263 1102 1 -1.2464372320027515 1102 2 -0.13760540830012502 1102 3 -0.03408904035379123 1102 4 0.17082135995887882 1102 5 -0.1491812867892719 1102 6 0.06554497974092754 1102 7 -0.09252414240421558 1102 8 0.43302426459781884 1102 9 0.5247243694367896 1102 10-11 0.40241999771443915 1102 12-13 0.20800783557903912 1102 14-15 -0.4592511662651475 1102 16-17 -0.377038762294589 1102 18-19 -0.5085302460625556 1102 20-21 0.5051144196273398 1102 22-23 0.5213080167650048 1102 24-25 -5.711621137426263 1102 26-27 -1.4780224643942432 1102 28-29 -0.9914591202568062 1102 30-31 1.4530982024944024 1102 32-33 -0.2983089304977895 1102 34-35 0.12996159633242144 1102 36-37 -3.4066656271722024 1102 38-39 -0.15570908079622825 1102 40-41 1.2513470195903622 1102 42-43 0.6779770584609892 1102 44-45 0.9208495573668074 1102 46-47 0.8270346744524417 1102 48-49 -4.238134071014365 1102 50-51 -0.8526532167239367 1102 52-53 -0.013377251753233566 1102 54-55 0.07961510023380924 1102 56-57 0.5603491075396576 1102 58-59 0.13445726300232508 1102 60-61 -7.517838711687425 1102 62-63 -2.719224364854732 1102 64-65 -0.16883852583075054 1102 66-67 1.5624111938157128 1102 68-69 -1.3118639057974697 1102 70-71 -1.0285314196315554 1102 72-73 -6.498348260163297 1102 74-75 -2.6918687889673762 1102 76 -1.5773028733724956 1103 1 0.6754944158920608 1103 2 0.43487972729227664 1103 3 0.21669647712095497 1103 4 0.48077447060808254 1103 5 0.23013905932181444 1103 6 0.2899854496012253 1103 7 0.8800673401693828 1103 8 0.448782953629582 1103 9 0.12558481319373271 1103 10-11 0.24045837930674452 1103 12-13 -0.4439716684573085 1103 14-15 -0.09892825815860817 1103 16-17 0.24194900780257456 1103 18-19 0.8145470488569657 1103 20-21 0.08170841585685196 1103 22-23 0.20872040135435554 1103 24-25 -3.2563110392970103 1103 26-27 -0.827203109243662 1103 28-29 -0.6076915367502522 1103 30-31 0.7388573749215368 1103 32-33 0.7679887443704914 1103 34-35 0.061947867903938914 1103 36-37 -1.2526075230488445 1103 38-39 0.05906348168261388 1103 40-41 -0.1763128634279525 1103 42-43 -0.08694777355911754 1103 44-45 1.4206320294507222 1103 46-47 1.1973155271618694 1103 48-49 -2.116366777545039 1103 50-51 -0.5757885190877445 1103 52-53 0.2869804608198834 1103 54-55 -0.22610830093601209 1103 56-57 0.21145849991167864 1103 58-59 -0.5327975346967122 1103 60-61 -3.6701082529452194 1103 62-63 -0.9272777317034837 1103 64-65 -0.1506386901851755 1103 66-67 -0.5216512608659727 1103 68-69 0.21262006964605007 1103 70-71 0.11312759327510236 1103 72-73 -4.170775388311409 1103 74-75 -2.5613520393178355 1103 76 -3.0498218466407288 1104 1 0.3458255417198757 1104 2 0.20657950654172197 1104 3 0.2079547552666483 1104 4 -0.15277961327712575 1104 5 -0.6046070775435268 1104 6 0.12724814275796348 1104 7 0.611855419639582 1104 8 0.5448977439165574 1104 9 0.8244810604343513 1104 10-11 -0.6182612674923789 1104 12-13 0.6116574706155333 1104 14-15 0.5866125806934903 1104 16-17 0.7334721866105198 1104 18-19 0.7103748634265017 1104 20-21 -0.08257414484955206 1104 22-23 0.7155415934073375 1104 24-25 0.6644615876345625 1104 26-27 1.2043862947298436 1104 28-29 1.848224578040032 1104 30-31 1.7218816574822426 1104 32-33 0.8081874198671812 1104 34-35 0.8597182873299971 1104 36-37 1.4759134482535856 1104 38-39 1.5998140335589994 1104 40-41 2.0792059003689474 1104 42-43 1.0524782750059956 1104 44-45 1.0180271729385808 1104 46-47 1.141730538199404 1104 48-49 0.17473697521434417 1104 50-51 0.9025779268283713 1104 52-53 0.5833380767139218 1104 54-55 0.49216984475935277 1104 56-57 0.30874326812359953 1104 58-59 -0.6771242455134896 1104 60-61 0.7564921885558888 1104 62-63 0.2139143212766541 1104 64-65 1.1350787491084162 1104 66-67 0.3809756707013534 1104 68-69 2.288403734105202 1104 70-71 1.536505076718818 1104 72-73 1.1166639052868277 1104 74-75 0.35489520130026264 1104 76 0.5481472482820067 1105 1 0.8283571872894981 1105 2 0.39510271329277913 1105 3 -0.07964018144221541 1105 4 -0.11902433901552456 1105 5 -0.10224420834521197 1105 6 0.13729033685078917 1105 7 0.8017288373611038 1105 8 -0.17729634891044554 1105 9 0.8860844359617772 1105 10-11 0.007266158667967204 1105 12-13 -1.139608352169276 1105 14-15 0.07311047098885126 1105 16-17 0.1875228195219094 1105 18-19 0.6302482811480132 1105 20-21 0.056371002829777694 1105 22-23 0.10959222631872478 1105 24-25 0.31133922476536213 1105 26-27 0.5862850289070565 1105 28-29 1.0491950421750573 1105 30-31 1.3991390414484854 1105 32-33 0.791984888221613 1105 34-35 -0.4010834004337198 1105 36-37 0.5230442499413499 1105 38-39 -0.3731395529388948 1105 40-41 0.654100415136881 1105 42-43 0.2934487391410272 1105 44-45 0.6879427847529271 1105 46-47 0.516076529760582 1105 48-49 -0.26610690664219305 1105 50-51 -0.07126173561889004 1105 52-53 0.2447304817772178 1105 54-55 0.8992584523542888 1105 56-57 0.3687432681236018 1105 58-59 0.20798123971858473 1105 60-61 0.6088128636613703 1105 62-63 -0.7833008685967684 1105 64-65 0.6942770613447067 1105 66-67 0.669752042009371 1105 68-69 0.2501336919111168 1105 70-71 1.144479760263117 1105 72-73 0.4570014580294419 1105 74-75 -0.4224887649444611 1105 76 0.7927885984929759 1106 1 -0.7654788061062092 1106 2 0.18715921668665203 1106 3 0.07867939294780513 1106 4 -0.08031584516118784 1106 5 0.1304653862245928 1106 6 0.1715959688449189 1106 7 -1.7939416818096916 1106 8 0.15475281638032357 1106 9 -1.8755189395656444 1106 10-11 0.29840539917429254 1106 12-13 0.918179209745972 1106 14-15 -0.727155535248535 1106 16-17 -0.13145534962136196 1106 18-19 0.12153428371635755 1106 20-21 0.26394759428088577 1106 22-23 0.663802462972555 1106 24-25 -1.1548137746842784 1106 26-27 1.0865602077733243 1106 28-29 0.6172100852864091 1106 30-31 1.1910120922648488 1106 32-33 0.5968830720410878 1106 34-35 0.6717472728372371 1106 36-37 1.219246781586918 1106 38-39 -0.3614903142670869 1106 40-41 1.3509450308037358 1106 42-43 1.220159434426293 1106 44-45 0.9816503613443786 1106 46-47 0.9698464802283908 1106 48-49 1.5550268302868062 1106 50-51 -0.2909003340411971 1106 52-53 0.4818888013516087 1106 54-55 -0.3881200103131093 1106 56-57 0.5571490652250546 1106 58-59 0.9060641602836128 1106 60-61 1.5246081305848733 1106 62-63 1.100436060407091 1106 64-65 -1.3089792219060712 1106 66-67 -0.11134316987836002 1106 68-69 1.0914472123660772 1106 70-71 0.17172246802315527 1106 72-73 0.06477984731581188 1106 74-75 -0.679597552322921 1106 76 0.1461182627747597 1107 1 0.9087202785619795 1107 2 -0.18192926538809928 1107 3 0.2845337026350663 1107 4 0.178360737600066 1107 5 0.02758590491261259 1107 6 0.3190025287228764 1107 7 0.7829080512185271 1107 8 0.3670907263726946 1107 9 -0.5180627992147677 1107 10-11 0.41024750443745006 1107 12-13 0.2511311548260622 1107 14-15 0.1916564403426122 1107 16-17 0.3851827129263157 1107 18-19 0.3515590739528136 1107 20-21 0.5760662060276402 1107 22-23 -0.18945840659266366 1107 24-25 0.07516334202052377 1107 26-27 -0.7596926526385772 1107 28-29 0.9259438762856433 1107 30-31 0.038504464499787616 1107 32-33 0.9388453146040163 1107 34-35 -0.09703609863491636 1107 36-37 0.8113520447448082 1107 38-39 -0.2903614050374941 1107 40-41 0.6044251986145639 1107 42-43 0.5846712574621478 1107 44-45 0.38701840100875984 1107 46-47 0.732432292585365 1107 48-49 0.685175571705571 1107 50-51 0.9587182777055645 1107 52-53 0.7313643925033944 1107 54-55 0.3676961605488316 1107 56-57 0.47729589970254693 1107 58-59 0.1458143509777443 1107 60-61 1.0964044692576422 1107 62-63 0.5419406370661264 1107 64-65 0.5410875210382429 1107 66-67 -1.6821383643863683 1107 68-69 -0.325982230807071 1107 70-71 -0.3441528180180313 1107 72-73 -0.0958799543622959 1107 74-75 -1.4096223425593841 1107 76 0.34691917810656747 1108 1 -0.3807534056485409 1108 2 0.3443865240855857 1108 3 -0.20230840262809124 1108 4 0.39546600075795624 1108 5 0.2841648522810374 1108 6 -0.1283658923297608 1108 7 -0.11182879088673303 1108 8 -0.41580401046940807 1108 9 -0.3009575360568775 1108 10-11 0.6339317149637722 1108 12-13 0.9945522074576445 1108 14-15 0.6653406508689343 1108 16-17 -0.052317287073684327 1108 18-19 0.21340117921597113 1108 20-21 0.832645153396065 1108 22-23 0.04738369867049386 1108 24-25 1.1047686051784211 1108 26-27 -0.5656137052701524 1108 28-29 -0.7714245447669867 1108 30-31 0.6273202539734655 1108 32-33 1.2085821567092836 1108 34-35 0.4819112697861314 1108 36-37 0.9659573079027055 1108 38-39 1.4366122791730334 1108 40-41 0.44324098808824886 1108 42-43 -0.010723479379961987 1108 44-45 0.8212289273245474 1108 46-47 1.1765112399537898 1108 48-49 1.2410966243371533 1108 50-51 0.8666130145476671 1108 52-53 0.3432064977665519 1108 54-55 -0.18493541839853833 1108 56-57 0.6680853733867593 1108 58-59 0.6129196141356417 1108 60-61 0.9648255218892174 1108 62-63 0.6537827423292839 1108 64-65 0.5476664684066606 1108 66-67 1.321151109297844 1108 68-69 1.7858598744560865 1108 70-71 -0.09415281801803133 1108 72-73 0.7593832035324404 1108 74-75 0.21537765744061588 1108 76 1.1561297044223586 1109 1 -0.5903034905381901 1109 2 0.29281606568150664 1109 3 0.25053540042793543 1109 4 0.2945322146798617 1109 5 0.0033499117037862902 1109 6 -0.3961927174570903 1109 7 -0.34620909648945286 1109 8 0.2380160234864448 1109 9 0.6846961141977914 1109 10-11 0.4871150765936463 1109 12-13 0.6535929544864985 1109 14-15 0.7699459140268274 1109 16-17 0.8037947672556811 1109 18-19 0.9371490569748886 1109 20-21 1.1789312314945377 1109 22-23 0.4145738514718573 1109 24-25 -0.08510830483855614 1109 26-27 0.49567661731049384 1109 28-29 -0.9531732714223295 1109 30-31 1.0013440230736421 1109 32-33 1.1639938714800806 1109 34-35 -0.27458278793882585 1109 36-37 1.2950532331998232 1109 38-39 1.6851903776450143 1109 40-41 0.6120015992936843 1109 42-43 0.9023707481242838 1109 44-45 0.5242637320783743 1109 46-47 0.78345096830693 1109 48-49 0.5341993408057419 1109 50-51 0.024083303172460546 1109 52-53 -0.23117805231833444 1109 54-55 0.3515246834690302 1109 56-57 0.7026142358655321 1109 58-59 0.42266070072307826 1109 60-61 0.9591803605988929 1109 62-63 0.3390756115992346 1109 64-65 0.7917454157750825 1109 66-67 0.5875348104862965 1109 68-69 -0.37428443793780275 1109 70-71 0.7839244315575229 1109 72-73 0.53591121711478 1109 74-75 0.33704573893467327 1109 76 0.719330043980932 1110 1 -1.0419163937639944 1110 2 -0.21686135367333037 1110 3 0.19892249720213329 1110 4 0.1913064082282503 1110 5 0.33883378267152864 1110 6 0.07477502447839157 1110 7 0.4279844518976432 1110 8 0.7154353783251608 1110 9 0.9363090174236035 1110 10-11 -0.2741752459870028 1110 12-13 0.792302631905855 1110 14-15 1.137687849510698 1110 16-17 0.5812141220943943 1110 18-19 -0.4047864268960808 1110 20-21 0.991834457300989 1110 22-23 -0.3467164711087918 1110 24-25 -0.5173663693546828 1110 26-27 0.9085798431169465 1110 28-29 0.25327834148089323 1110 30-31 -0.35672049305539133 1110 32-33 0.7188325811575069 1110 34-35 0.9705785023837592 1110 36-37 1.6627951686836937 1110 38-39 0.7206742486127595 1110 40-41 -1.6299338845772837 1110 42-43 0.16043526425330867 1110 44-45 1.1500701836912768 1110 46-47 0.7447412908875677 1110 48-49 1.285812244031547 1110 50-51 -0.3791425032791551 1110 52-53 0.45269291542360435 1110 54-55 -0.32266886491806446 1110 56-57 0.33164649393005163 1110 58-59 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38-39 -4.561519299774336 2116 40-41 -0.9721274329643848 2116 42-43 -1.5528550583273315 2116 44-45 -1.3686394937280824 2116 46-47 -1.3849361284672668 2116 48-49 0.1420703085476802 2116 50-51 -1.1947554065049601 2116 52-53 -0.6346619232860817 2116 54-55 -0.9638301552406432 2116 56-57 -0.2732567318763941 2116 58-59 -2.6757909121801546 2116 60-61 -1.6041744781107852 2116 62-63 0.085914321276654 2116 64-65 -1.8842545842249123 2116 66-67 -0.751690995965312 2116 68-69 -1.446929599228124 2116 70-71 0.8505050767188109 2116 72-73 0.14533057195349386 2116 74-75 0.10956186796693146 2116 76 -2.505186085051328 2117 1 0.6762131386191044 2117 2 0.47061051429366074 2117 3 0.14810979402633961 2117 4 -0.005492791571704458 2117 5 0.046710751913835225 2117 6 -0.05910844638932389 2117 7 0.47619650491090226 2117 8 0.3493938679475619 2117 9 -1.0034259163098298 2117 10-11 -0.2539201822210586 2117 12-13 -0.84260609527594 2117 14-15 0.038938162088840045 2117 16-17 -0.525287503311958 2117 18-19 -1.0790049815347373 2117 20-21 0.247348335770603 2117 22-23 0.6622857794538461 2117 24-25 -1.8954608929855894 2117 26-27 -2.5074741703864305 2117 28-29 -1.1423955769987302 2117 30-31 0.25420723887759067 2117 32-33 -2.102897851450642 2117 34-35 0.5863074346168133 2117 36-37 -1.0230012804285877 2117 38-39 0.4115969792954317 2117 40-41 -0.15916619265430398 2117 42-43 -0.6481418800327532 2117 44-45 -0.7196472456660672 2117 46-47 -0.9610601594750179 2117 48-49 -0.4641002340879794 2117 50-51 -0.31137556154372703 2117 52-53 0.38760164260539653 2117 54-55 -0.4019386823724318 2117 56-57 0.27765799680577175 2117 58-59 -1.4380544780716278 2117 60-61 -0.7260659509789988 2117 62-63 -0.29600815934350067 2117 64-65 -0.8842235764729764 2117 66-67 -1.2296444843374061 2117 68-69 0.4729773775160595 2117 70-71 -0.8818670163044438 2117 72-73 1.438136773503878 2117 74-75 1.1449727206801121 2117 76 1.363341046731616 2118 1 0.1696211621578314 2118 2 0.563124518707177 2118 3 -0.6107313761202136 2118 4 0.14162427966691382 2118 5 0.3617676183202221 2118 6 0.3356179724416606 2118 7 0.9147751276687757 2118 8 0.3527322937949009 2118 9 -1.0519179663296399 2118 10-11 -0.8421055497308174 2118 12-13 -0.7992914344939521 2118 14-15 0.6611867899392294 2118 16-17 -2.6106154046303587 2118 18-19 -0.2895521438727684 2118 20-21 -0.1299220767230267 2118 22-23 0.8315269948671897 2118 24-25 1.0219555049095064 2118 26-27 -2.649555311109566 2118 28-29 -0.7067875874100906 2118 30-31 -1.371719315753765 2118 32-33 -0.1815206093299011 2118 34-35 0.19565502698935688 2118 36-37 -2.9541108826466598 2118 38-39 0.03772157613806826 2118 40-41 0.10901125316700444 2118 42-43 0.7327215840084307 2118 44-45 -1.8966686908083759 2118 46-47 0.294187959123974 2118 48-49 0.6122308924892863 2118 50-51 -0.5059378882567884 2118 52-53 -1.1484137481036 2118 54-55 -0.7415527829778767 2118 56-57 0.6844366987805373 2118 58-59 -0.24145514575679528 2118 60-61 -0.266013894169177 2118 62-63 -0.9272535619350251 2118 64-65 0.25451913840282714 2118 66-67 0.8543819967354196 2118 68-69 -0.8556595262354278 2118 70-71 0.35468025920056334 2118 72-73 1.9031115938512997 2118 74-75 1.6365983643172974 2118 76 1.751164279912178 2119 1 0.621825541719879 2119 2 -0.5440871601249384 2119 3 0.015954755266648135 2119 4 -0.022112946610462814 2119 5 0.03405958912313878 2119 6 -0.363418523908706 2119 7 -0.2001445803604156 2119 8 -0.08843558941677543 2119 9 0.06714772710102324 2119 10-11 -0.1755946008257112 2119 12-13 -0.5883425293844624 2119 14-15 -0.5300540859731697 2119 16-17 -0.3065278133894722 2119 18-19 -0.5556251365734965 2119 20-21 -1.5359074781828852 2119 22-23 -0.31845840659266145 2119 24-25 1.1197949209678981 2119 26-27 0.6303862947298455 2119 28-29 0.4848912447067022 2119 30-31 0.5252149908155701 2119 32-33 1.0501874198671786 2119 34-35 0.28838495399665476 2119 36-37 0.21324678158691768 2119 38-39 -0.9855192997743387 2119 40-41 -0.9601274329643843 2119 42-43 -0.10485505832733111 2119 44-45 0.23936050627191463 2119 46-47 -0.10493612846726563 2119 48-49 -0.6379296914523138 2119 50-51 0.8732445934950377 2119 52-53 0.3093380767139209 2119 54-55 0.06816984475935328 2119 56-57 -1.8452567318763968 2119 58-59 -0.23179091218015202 2119 60-61 0.38782552188921926 2119 62-63 0.20991432127665632 2119 64-65 -0.06825458422491693 2119 66-67 0.9923090040346878 2119 68-69 0.7490704007718705 2119 70-71 0.1705050767188112 2119 72-73 1.5213305719534915 2119 74-75 0.5815618679669328 2119 76 0.47081391494867475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 12.0 23 19.0 24 45.0 25 108.0 26 187.0 27 371.0 28 590.0 29 920.0 30 1315.0 31 2041.0 32 3124.0 33 4448.0 34 6252.0 35 7794.0 36 9376.0 37 15913.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.247024772930672 22.18900546489708 10.356646419254718 36.20732334291753 2 16.00814974198831 16.746958127844316 47.715977683416796 19.528914446750576 3 15.075118533046442 27.021726298151076 38.50943504008226 19.393720128720222 4 10.6746386884247 25.399394481786853 32.05438239046404 31.87158443932441 5 9.903459832054383 39.42913723175352 37.47548412894872 13.191918807243368 6 20.206790182226708 38.800769274711044 15.726336233981378 25.266104309080866 7 20.509549288801722 37.35742711883771 16.59081821124588 25.542205381114684 8 17.996077460631795 36.85282860787935 23.847516042424356 21.303577889064492 9 17.042100653121846 21.06746386884247 15.96054610887903 45.929889369156655 10-11 17.981796370699012 24.572995411009767 31.632614201115828 25.81259401717539 12-13 22.310870765656833 17.639050212312206 27.871927185482797 32.17815183654816 14-15 23.548565226498084 22.179484738275228 18.79010606089457 35.48184397433212 16-17 25.506026619951633 27.123598073004935 18.22552697221852 29.144848334824914 18-19 22.765009425519356 26.065845345316756 18.57208142125407 32.59706380790982 20-21 21.103642630005524 22.317535274292133 18.93577317820896 37.64304891749338 22-23 23.6532932193385 30.41205704819392 19.07572785955024 26.858921872917342 24-25 25.090922939238723 33.05596283108327 19.02907629910315 22.82403793057486 26-27 22.33752880019803 28.99061256355085 28.743073671382604 19.92878496486852 28-29 16.523221052230706 21.298817525753567 30.137860121484472 32.040101300531255 30-31 15.836776662794904 24.326408591503704 32.09532151493802 27.74149323076337 32-33 15.274101719443228 32.29144848334825 27.535845535731283 24.898604261477235 34-35 15.772035721766287 33.50629320029705 33.31683074052212 17.40484033741455 36-37 15.900565531161337 28.74116952605823 33.71955747662662 21.638707466153818 38-39 20.197269455604854 21.094121903383666 29.34192737589733 29.366681265114153 40-41 18.140792505284004 23.350534112763484 32.229563760306185 26.279109621646324 42-43 26.30767180151189 21.374983338728413 33.60245253917779 18.714892320581907 44-45 18.71203610259535 24.47397985414247 30.117866595578572 26.69611744768361 46-47 26.28863034826818 18.469257573738027 27.377801473808482 27.86431060418531 48-49 22.717405792410077 18.226479044880705 28.827808138317117 30.228307024392105 50-51 15.833920444808347 18.3654816535598 35.56467429594227 30.235923605689585 52-53 18.336919473694234 18.642534798255802 41.167621912904394 21.852923815145573 54-55 16.396595388160023 18.906258925681207 35.76746577298779 28.929679913170975 56-57 24.319744082868404 18.854847001923186 30.570101110116727 26.255307805091682 58-59 16.913570843726795 28.08614353447455 34.642115886284444 20.358169735514213 60-61 19.511777138831235 38.84646876249596 23.148694708380145 18.493059390292668 62-63 13.891692213949769 47.04191023858941 19.830721480663406 19.23567606679742 64-65 13.274749128853514 48.21867204905078 18.995753755926653 19.510825066169048 66-67 13.22143305977112 47.46367842793762 18.987185101966983 20.327703410324276 68-69 13.619399432564695 44.54462364567664 19.028124226440962 22.807852695317706 70-71 14.16874535864577 41.36374888131462 19.934497400841632 24.533008359197975 72-73 14.270617133499627 37.25555534398385 21.626330521545402 26.847497000971117 74-75 14.40390730620561 36.51484281280347 21.529219110002476 27.552030770988438 76 13.827903345583334 37.427880495839446 21.954795589999428 26.789420568577793 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 1.0 14 0.5 15 2.0 16 3.0 17 3.0 18 6.0 19 14.5 20 16.5 21 13.0 22 25.0 23 38.0 24 48.0 25 57.0 26 90.0 27 139.5 28 209.0 29 262.0 30 262.0 31 321.5 32 452.5 33 524.0 34 611.5 35 854.0 36 1309.5 37 1610.0 38 2704.0 39 4696.0 40 5662.0 41 5097.0 42 4464.0 43 4087.5 44 3422.5 45 3027.0 46 2920.0 47 2808.0 48 2561.0 49 2380.5 50 2335.0 51 2169.5 52 1839.0 53 1586.5 54 1499.0 55 1429.0 56 1240.0 57 984.0 58 847.0 59 770.5 60 597.5 61 410.0 62 319.0 63 283.5 64 187.0 65 91.5 66 43.5 67 30.0 68 20.0 69 7.5 70 3.5 71 2.0 72 1.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 52517.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.88163832663709 #Duplication Level Percentage of deduplicated Percentage of total 1 67.92730454147777 32.52470628558372 2 18.332935655770303 17.556219890702057 3 7.464407858108646 10.72224232153398 4 2.9189533126541 5.590570672353714 5 1.276544977332379 3.0561532456157057 6 0.7158196134574087 2.0564769503208487 7 0.39370078740157477 1.3195727097892112 8 0.2107691084069037 0.8073576175333701 9 0.1511174739521196 0.6512177009349354 >10 0.5169808319414618 3.9244435135289524 >50 0.043744531933508315 1.4014509587371708 >100 0.0318142050425515 2.9571376887484053 >500 0.003976775630318938 1.03775920178228 >1k 0.011930326890956812 16.394691242835655 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGT 3887 7.401412875830683 No Hit ATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCTT 3536 6.733057866976408 Illumina PCR Primer Index 2 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 1187 2.2602205000285625 No Hit CTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 545 1.03775920178228 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 390 0.7426166765047508 No Hit TATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTCT 326 0.6207513757449968 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 172 0.3275129957918388 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 159 0.3027591065750138 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 155 0.29514252527752916 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGT 132 0.2513471828169926 No Hit CTTATAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 118 0.22468914827579642 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCC 101 0.19231867776148676 No Hit CTTAACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 99 0.18851038711274445 No Hit CTTATTACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 76 0.14471504465220786 No Hit TTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCGTC 76 0.14471504465220786 No Hit CTTATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGT 70 0.13329017270598092 No Hit ATACACATCTCCGAGCCCACGAGACTTCCATATATATCGTATGCCGTCTT 64 0.12186530075975399 No Hit ATACACATCTCCGAGCCCACGAGACTTCCCTATATCTCGTATGCCGTCTT 62 0.11805701011101168 Illumina PCR Primer Index 6 (95% over 22bp) CTTTATACACATCTCCGAGCCCACGAGACTTCCATATATCTCGTATGCCG 61 0.11615286478664053 No Hit ATACACATCTCCGAGCCCACGAGACTTCCATATACCTCGTATGCCGTCTT 61 0.11615286478664053 No Hit ATACACATCTCCGAGCCCACGAGACTTCCATATAACTCGTATGCCGTCTT 58 0.11044042881352704 No Hit CTTATACACATCTCCGAGCCCACGAGCCTTCCATATATCTCGTATGCCGT 58 0.11044042881352704 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.020945598568082716 0.0 2 0.0 0.0 0.0 0.020945598568082716 0.0 3 0.0 0.0 0.0 0.020945598568082716 0.0 4 0.0 0.0 0.0 0.022849743892453873 0.0 5 0.0 0.0 0.0 0.024753889216825028 0.0 6 0.0 0.0 0.0 0.024753889216825028 0.0 7 0.0 0.0 0.0 0.024753889216825028 0.0 8 0.0 0.0 0.0 0.026658034541196186 0.0 9 0.0 0.0 0.0 0.03427461583868081 0.0 10 0.0 0.0 0.0 0.03427461583868081 0.0 11 0.0 0.0 0.0 0.03427461583868081 0.0 12 0.0 0.0 0.0 0.03427461583868081 0.0 13 0.0 0.0 0.0 0.03808290648742312 0.0 14 0.0 0.0 0.0 0.03808290648742312 0.0 15 0.0 0.0 0.0 0.03808290648742312 0.0 16 0.0 0.0 0.0 0.03998705181179427 0.0 17 0.0 0.0 0.0 0.03998705181179427 0.0 18 0.0 0.0 0.0 0.04189119713616543 0.0 19 0.0 0.0 0.0 0.049507778433650056 0.0 20 0.0 0.0 0.0 0.051411923758021214 0.0 21 0.0 0.0 0.0 0.05331606908239237 0.0 22 0.0 0.0 0.0 0.05331606908239237 0.0 23 0.0 0.0 0.0 0.06664508635299046 0.0 24 0.0 0.0 0.0 0.06854923167736161 0.0 25 0.0 0.0 0.0 0.07997410362358855 0.0 26 0.0 0.0 0.0 0.08568653959670203 0.0 27 0.0 0.0 0.0 0.11615286478664051 0.0 28 0.0 0.0 0.0 0.1428108993278367 0.0 29 0.0 0.0 0.0 0.17708551516651752 0.0 30 0.0 0.0 0.0 0.21136013100519832 0.0 31 0.0 0.0 0.0 0.268484490736333 0.0 32 0.0 0.0 0.0 0.3332254317649523 0.0 33 0.0 0.0 0.0 0.39606222746920045 0.0 34 0.0 0.0 0.0 0.49126949368775824 0.0 35 0.0 0.0 0.0 0.5902850505550583 0.0 36 0.0 0.0 0.0 0.7864120189652875 0.0 37 0.0 0.0 0.0 1.1310623226764667 0.0 38 0.0 0.0 0.0 1.477616771712017 0.0 39 0.0 0.0 0.0 1.915570196317383 0.0 40 0.0 0.0 0.0 2.4315935792219663 0.0 41 0.0 0.0 0.0 3.1208941866443247 0.0 42 0.0 0.0 0.0 3.861606717824704 0.0 43 0.0 0.0 0.0 4.6042233943294555 0.0 44 0.0 0.0 0.0 5.348744216158577 0.0 45 0.0 0.0 0.0 6.100881619285184 0.0 46 0.0 0.0 0.0 6.856827313060533 0.0 47 0.0 0.0 0.0 7.508045013995468 0.0 48 0.0 0.0 0.0 8.163071005579146 0.0 49 0.0 0.0 0.0 8.74764362016109 0.0 50 0.0 0.0 0.0 9.376011577203572 0.0 51 0.0 0.0 0.0 10.03294171411162 0.0 52 0.0 0.0 0.0 10.619418474017937 0.0 53 0.0 0.0 0.0 11.31823980806215 0.0 54 0.0 0.0 0.0 12.08941866443247 0.0 55 0.0 0.0 0.0 12.786335853152313 0.0 56 0.0 0.0 0.0 13.378525049031742 0.0 57 0.0 0.0 0.0 14.01641373269608 0.0 58 0.0 0.0 0.0 14.673343869604128 0.0 59 0.0 0.0 0.0 15.21983357769865 0.0 60 0.0 0.0 0.0 15.629224822438449 0.0 61 0.0 0.0 0.0 16.187139402479197 0.0 62 0.0 0.0 0.0 16.65175086162576 0.0 63 0.0 0.0 0.0 17.232515185558963 0.0 64 0.0 0.0 0.0 17.82280023611402 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGCTGG 15 0.002213064 70.00001 1 AGACTCT 15 0.002213064 70.00001 6 CTATACA 105 0.0 66.66667 1 TTTACAC 145 0.0 55.517242 2 TTACACA 150 0.0 53.666668 3 TACTAAT 20 0.0069153253 52.5 4 AGCTCGT 20 0.0069153253 52.5 36 AACTAAA 20 0.0069153253 52.5 70 CATCAGG 20 0.0069153253 52.5 26 TAGCTCG 20 0.0069153253 52.5 35 CTTTACA 155 0.0 51.935486 1 ATAACAC 35 2.0601143E-5 50.0 4 TATAACA 35 2.0601143E-5 50.0 3 TTAATAC 30 5.8280025E-4 46.666668 2 ATATCGT 30 5.8280025E-4 46.666668 37 GAGCCTT 30 5.8280025E-4 46.666668 24 TTATAAC 40 4.5348737E-5 43.75 2 CTTATAA 40 4.5348737E-5 43.75 1 TAATACA 35 0.0012448562 40.0 3 ACTTCCC 35 0.0012448562 40.0 27 >>END_MODULE