FastQCFastQC Report
Wed 25 May 2016
SRR1780445_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780445_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412550
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTG250.002362057835.166358
CCCGGAT300.005702940729.37293816
CGTCGAA300.00570971729.36580519
CTCGATT300.00576757729.3052927
TCAACGT406.96902E-427.5371315
ACTCGCA406.9739716E-427.53378721
TTACTCG406.978926E-427.53044319
TCCGTAT406.983884E-427.52709813
CGTATTA450.001385713624.47745115
TACTCGC500.002561069822.02435520
GTGCAAC602.870419E-421.99763523
GGTATAC602.8774937E-421.9896321
TCCAACT500.00259193621.978979
CGGTGGT1750.021.35617843
TTTACAC2450.020.6308172
CTTTACA2400.020.1571621
CCGTATT550.004443839720.02700414
TCCACGA550.004456355220.01727712
CATCGTG550.00446576120.00998739
CGCATCA550.004475183320.00270324
Produced by FastQC (version 0.11.5)