FastQCFastQC Report
Wed 25 May 2016
SRR1780445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412550
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT346308.394134044358259No Hit
ATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCTT279356.771300448430494RNA PCR Primer, Index 24 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC81201.9682462731790085No Hit
CTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC42831.0381771906435584No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG41591.0081202278511696No Hit
TATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTCT27100.6568900739304326No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC19520.47315476911889465No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC12660.3068718943158405No Hit
CTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG11500.2787540904132832No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC8220.19924857593019027No Hit
CTTAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC7010.1699187977214883No Hit
CTTATAACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG6960.1687068234153436No Hit
TTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGTC6250.1514967882680887No Hit
CTTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG4810.11659192825112107No Hit
CTTATTACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG4670.11319840019391589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT29450.041.9113244
GCCGTCT32400.038.02741243
TTTACAC10050.037.8733562
CTTATAA950.037.1002581
TTCGGTA301.3000284E-436.66485226
CTATACA5950.036.652091
CTTTACA10500.036.294211
TACGGAC250.002351574835.19825438
CTAAATG250.002351574835.19825436
TCGTCTC250.002351574835.1982549
TTACACA11100.034.2907453
TATCGCG401.8177989E-532.99836336
ATCGCGT401.8177989E-532.99836337
GGTCCGT353.1900906E-431.4689661
TCGTTCG353.2151528E-431.42701123
CGAGAAT353.2151528E-431.42701114
CTTATAC50050.030.8968051
CTTTATA2000.030.8022042
TTATACA50250.030.5176092
GATCAGT300.00574207529.3318822
Produced by FastQC (version 0.11.5)