FastQCFastQC Report
Wed 25 May 2016
SRR1780444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69790
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGC207.880201E-569.99354649
CTACTGA150.00221693969.9935463
ACTGAGT150.00221693969.9935465
TGACGGG150.00221693969.9935462
TCTACTG150.00221693969.9935462
CGACGAT200.00692735452.4951622
TCATTGA200.00692735452.4951625
GACGTCG200.00692735452.4951623
CGCGCTT200.00692735452.4951618
TCCTCAG200.00692735452.4951616
GCGCACC200.00692735452.4951668
GGGACAG200.00692735452.4951611
TCACTAG200.00692735452.4951613
TCGTACT200.00692735452.4951623
GAGTTGA200.00692735452.495169
CAATGTA200.00692735452.4951645
ACGTAGA200.00692735452.495162
TATAATG200.00692735452.495163
TGTGTAC200.00692735452.4951612
AATGTAT200.00692735452.4951646
Produced by FastQC (version 0.11.5)