FastQCFastQC Report
Wed 25 May 2016
SRR1780444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780444_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69790
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT39885.714285714285714No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT35005.015045135406218RNA PCR Primer, Index 15 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC11821.6936523857286143No Hit
CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC5960.8539905430577447No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG4360.6247313368677461No Hit
TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT3010.43129388164493476No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC1970.2822753976214358No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC1870.2679466972345608No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT1670.239289296460811No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG1430.2049004155323112No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC1120.160481444332999No Hit
CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG1110.1590485742943115No Hit
CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC950.13612265367531165No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT940.13468978363662415No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT860.12322682332712423No Hit
TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC800.11462960309499928No Hit
CTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGT780.1117638630176243No Hit
ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT780.1117638630176243No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG700.10030090270812438No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGGA150.002216129370.035
ATATGGA207.876604E-570.04
ATATATG207.876604E-570.02
CTTGAAC252.3754459E-455.99999654
ATGCAGT252.3754459E-455.99999644
AGTTTTC200.00692482952.568
CTATAAC200.00692482952.54
GTGGATG200.00692482952.512
TCGTGCC200.00692482952.59
GCGGACC200.00692482952.527
GACTATC200.00692482952.538
GACTACT200.00692482952.544
TTTACAC2100.051.6666642
TTAATAC552.233719E-950.909093
CTTATAA352.0671803E-550.01
GAACAAA352.0671803E-550.057
CTTTACA2250.049.777781
TTACACA2250.049.777783
CTATACA1100.047.7272721
CGTCTGC451.6629583E-646.66666438
Produced by FastQC (version 0.11.5)