##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780444_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 69790 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.86046711563261 34.0 34.0 34.0 31.0 34.0 2 33.30412666571142 34.0 34.0 34.0 33.0 34.0 3 33.5070927066915 34.0 34.0 34.0 34.0 34.0 4 33.47671586187133 34.0 34.0 34.0 34.0 34.0 5 33.53255480727898 34.0 34.0 34.0 34.0 34.0 6 37.272775469264936 38.0 37.0 38.0 36.0 38.0 7 36.90444189711993 38.0 37.0 38.0 35.0 38.0 8 36.92169365238573 38.0 37.0 38.0 35.0 38.0 9 36.72738214643932 38.0 37.0 38.0 34.0 38.0 10-11 36.45046568276257 38.0 37.0 38.0 34.0 38.0 12-13 36.28490471414243 38.0 37.0 38.0 34.0 38.0 14-15 36.48789941252329 38.0 37.0 38.0 34.0 38.0 16-17 36.58105029373836 38.0 37.0 38.0 34.0 38.0 18-19 36.610187705975065 38.0 37.0 38.0 34.0 38.0 20-21 36.365052299756414 38.0 37.0 38.0 34.0 38.0 22-23 36.4174666857716 38.0 37.0 38.0 34.0 38.0 24-25 35.7382146439318 38.0 36.5 38.0 32.0 38.0 26-27 35.90744375985098 38.0 37.0 38.0 31.5 38.0 28-29 35.7698739074366 38.0 36.5 38.0 31.5 38.0 30-31 35.366599799398195 38.0 36.0 38.0 30.5 38.0 32-33 35.859936953718304 38.0 36.5 38.0 31.0 38.0 34-35 35.72348473993409 38.0 36.5 38.0 31.0 38.0 36-37 35.77420833930363 38.0 37.0 38.0 31.0 38.0 38-39 35.19135979366672 38.0 35.5 38.0 29.0 38.0 40-41 35.14565123943258 37.0 35.5 38.0 29.0 38.0 42-43 35.743136552514684 38.0 36.5 38.0 32.5 38.0 44-45 35.961391316807564 38.0 37.0 38.0 33.0 38.0 46-47 36.175755838945406 38.0 37.0 38.0 34.0 38.0 48-49 35.89393179538616 38.0 36.5 38.0 32.5 38.0 50-51 35.97820604671156 38.0 37.0 38.0 32.5 38.0 52-53 35.850501504513545 38.0 37.0 38.0 32.0 38.0 54-55 36.09729187562688 38.0 37.0 38.0 34.0 38.0 56-57 36.05362516119788 38.0 37.0 38.0 33.0 38.0 58-59 36.06061756698667 38.0 37.0 38.0 34.0 38.0 60-61 35.83403066341883 38.0 37.0 38.0 32.5 38.0 62-63 36.21573291302479 38.0 37.0 38.0 34.0 38.0 64-65 36.18075655538043 38.0 37.0 38.0 34.0 38.0 66-67 35.26576873477576 38.0 36.0 38.0 29.5 38.0 68-69 33.78793523427426 37.0 34.0 38.0 22.5 38.0 70-71 32.860323828628744 37.0 32.5 38.0 16.0 38.0 72-73 31.9067703109328 37.0 28.5 38.0 11.0 38.0 74-75 32.24047857859293 37.0 31.0 38.0 11.0 38.0 76 31.921335434876056 37.0 29.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.273327703733635 1101 2 0.23228862500649683 1101 3 0.14487262704798098 1101 4 0.38177083026139513 1101 5 -0.008362566188267806 1101 6 -1.0949071869527387 1101 7 0.08361890462502686 1101 8 0.2691876159418243 1101 9 0.6311783721288506 1101 10-11 0.2042483959023258 1101 12-13 0.651765755053809 1101 14-15 0.45673423310530126 1101 16-17 -0.09127590799652552 1101 18-19 -1.2102160231138441 1101 20-21 -0.5133717956640496 1101 22-23 0.3045233417893911 1101 24-25 -2.2468295067224417 1101 26-27 -0.9484906846095598 1101 28-29 0.39123071607758675 1101 30-31 0.6699482791791596 1101 32-33 -0.13909057957680915 1101 34-35 -0.1954860781325749 1101 36-37 -0.25241867624516345 1101 38-39 0.07221092486151548 1101 40-41 0.5412832796252403 1101 42-43 0.5525010845171323 1101 44-45 0.6189620389221204 1101 46-47 0.1848171630886739 1101 48-49 -2.131119731991575 1101 50-51 -2.322812149943843 1101 52-53 -2.9755696416436237 1101 54-55 -2.0611590595652203 1101 56-57 -1.8419941360947973 1101 58-59 -1.3783106527713898 1101 60-61 -2.6772796345995715 1101 62-63 -1.481499973655822 1101 64-65 -1.619364144921164 1101 66-67 -2.1996882387986574 1101 68-69 -1.1779712771833388 1101 70-71 -1.6981677615516233 1101 72-73 -3.4412388068865596 1101 74-75 -2.1801758076323168 1101 76 -1.3874546439307345 1102 1 -0.9865121670865236 1102 2 0.2772660858152207 1102 3 0.1082380310713944 1102 4 0.18803470700229497 1102 5 0.201386475055088 1102 6 0.11871052145068006 1102 7 -0.38348060422244146 1102 8 0.08392891985086237 1102 9 0.5505755328635615 1102 10-11 0.4962991259030005 1102 12-13 0.5410377728160327 1102 14-15 0.16286691156859945 1102 16-17 0.32966871960221056 1102 18-19 0.25915422515366515 1102 20-21 0.30471674635304424 1102 22-23 0.5891395027259421 1102 24-25 -4.205838455117792 1102 26-27 -1.0627677484593931 1102 28-29 0.29826496219209275 1102 30-31 1.539288250921075 1102 32-33 0.3290349670826771 1102 34-35 0.7050891758538427 1102 36-37 -3.007330201509241 1102 38-39 -0.4089167075141802 1102 40-41 0.7880537835611889 1102 42-43 0.8529283199339446 1102 44-45 0.8255320449774217 1102 46-47 1.0725417139868725 1102 48-49 -3.3576722449431955 1102 50-51 -0.5714160657078224 1102 52-53 0.07149353537312919 1102 54-55 0.5558597118806503 1102 56-57 0.6757344516734918 1102 58-59 0.062465099095661 1102 60-61 -5.586921025974121 1102 62-63 -2.299302570708903 1102 64-65 -0.027592631767703324 1102 66-67 0.7332997178722707 1102 68-69 -1.0487241534532785 1102 70-71 -2.2257193998480638 1102 72-73 -5.250328492683504 1102 74-75 -1.9721256158809837 1102 76 -1.2192517239280427 1103 1 0.7346847823225673 1103 2 0.4801245593554171 1103 3 0.18418260812210718 1103 4 0.39514304955338275 1103 5 0.3367710008744993 1103 6 0.3109443224636621 1103 7 0.8454010398364744 1103 8 0.5662308352027878 1103 9 0.24633148559104256 1103 10-11 0.30745648749116583 1103 12-13 -0.7038390974174149 1103 14-15 -0.27831897653758375 1103 16-17 0.255936403132516 1103 18-19 0.6869792236982306 1103 20-21 0.07547124364593571 1103 22-23 0.5116376106609692 1103 24-25 -2.3882771813214347 1103 26-27 -0.04923220674663753 1103 28-29 0.17797329309970422 1103 30-31 0.7513450710875702 1103 32-33 1.3161543709366654 1103 34-35 0.6129747204774674 1103 36-37 -0.6773977336743471 1103 38-39 0.20402501688472796 1103 40-41 -0.26651045932173645 1103 42-43 -0.4906561243811325 1103 44-45 0.7068122452452172 1103 46-47 0.6334225430024176 1103 48-49 -1.892064164370339 1103 50-51 -0.2837348641010209 1103 52-53 0.24172116534343502 1103 54-55 -0.13225520923479195 1103 56-57 0.47220647822061323 1103 58-59 -0.17274768544179864 1103 60-61 -3.6246749993105567 1103 62-63 -0.8549292808445514 1103 64-65 0.40193522525041203 1103 66-67 -0.14275547220749019 1103 68-69 0.28489056390132816 1103 70-71 0.31642998595853555 1103 72-73 -3.889409822368549 1103 74-75 -1.296352780112226 1103 76 -2.005710360476918 1104 1 0.23135012043868386 1104 2 0.20532716331450018 1104 3 0.11616081989306082 1104 4 -0.11127384960963127 1104 5 -0.37974288247876586 1104 6 0.11006747909977577 1104 7 0.6076170621561232 1104 8 0.392110999943057 1104 9 0.8308405861623172 1104 10-11 -0.6360402575599764 1104 12-13 0.5159297347866456 1104 14-15 0.5914033563971799 1104 16-17 0.10405650410235268 1104 18-19 0.2869127961706681 1104 20-21 0.006373329470299893 1104 22-23 0.2278459273874205 1104 24-25 0.24165506260044367 1104 26-27 0.9729280164498562 1104 28-29 1.414614717305895 1104 30-31 0.7421116447557026 1104 32-33 0.6658687325681356 1104 34-35 0.15578061924190933 1104 36-37 1.1531855000176918 1104 38-39 1.31349758231584 1104 40-41 1.655224627698324 1104 42-43 0.557782828721038 1104 44-45 0.6339013909872193 1104 46-47 0.658439149884309 1104 48-49 -0.00787391593138409 1104 50-51 0.40444103599197234 1104 52-53 0.48226852817059296 1104 54-55 0.3174990089132095 1104 56-57 0.0954693983747319 1104 58-59 -0.061375292722253505 1104 60-61 0.5555163119688302 1104 62-63 0.6208645281098839 1104 64-65 0.824640154172954 1104 66-67 0.35882550294861915 1104 68-69 1.7386570047143977 1104 70-71 1.8319873129148334 1104 72-73 0.8114404499843175 1104 74-75 0.966407947933476 1104 76 0.6770317678610631 1105 1 1.0242895481607235 1105 2 0.4361707158971839 1105 3 0.35882154081465245 1105 4 -0.07953843526551907 1105 5 -0.08371909949845957 1105 6 0.14574194955685016 1105 7 0.7943134575481707 1105 8 -0.18581542146162633 1105 9 0.6949059893581548 1105 10-11 0.7718378546659537 1105 12-13 -0.06623302998480796 1105 14-15 0.3964200788052068 1105 16-17 0.12880566798195758 1105 18-19 0.8000677571517159 1105 20-21 0.42417340379211055 1105 22-23 0.35040259777018434 1105 24-25 0.47093785710062974 1105 26-27 0.5437567046698462 1105 28-29 1.0548599792902849 1105 30-31 1.3957719940682267 1105 32-33 1.139150040632046 1105 34-35 -0.24968203404682754 1105 36-37 0.5025351841499841 1105 38-39 0.8437837212976262 1105 40-41 1.41546245750137 1105 42-43 0.7442190977660061 1105 44-45 1.1922068536405064 1105 46-47 0.9167446125375989 1105 48-49 -0.3465584198332863 1105 50-51 0.9257342355460381 1105 52-53 0.6975044999283071 1105 54-55 0.7827907220310095 1105 56-57 0.8913073768214019 1105 58-59 0.6341282375638784 1105 60-61 0.9790020050197441 1105 62-63 -0.22711020247354696 1105 64-65 0.6410281140391731 1105 66-67 0.8821625523726198 1105 68-69 0.6466837605672424 1105 70-71 1.1412403786896306 1105 72-73 1.4012212006346303 1105 74-75 1.0885186874355242 1105 76 1.3756568143121974 1106 1 -0.9219664904866214 1106 2 -0.22023025028639154 1106 3 0.18249303839919406 1106 4 0.17166929420307753 1106 5 0.2321021565401864 1106 6 0.3015390510061309 1106 7 -0.996145479649897 1106 8 0.04063385390069385 1106 9 -0.39446599131817095 1106 10-11 0.5692412363084642 1106 12-13 1.239926390305051 1106 14-15 -0.02658716757160562 1106 16-17 0.2764505955181846 1106 18-19 0.5283286267169345 1106 20-21 0.09114200282592577 1106 22-23 0.7251006654030334 1106 24-25 0.25294268801849995 1106 26-27 1.100519989694007 1106 28-29 0.5657295445076755 1106 30-31 0.6010860037300603 1106 32-33 0.41602202130837185 1106 34-35 0.9332889804459228 1106 36-37 1.3859892904301816 1106 38-39 0.5759697116357927 1106 40-41 1.1271170468733445 1106 42-43 0.8462722378626211 1106 44-45 0.8751778681332567 1106 46-47 0.9692808444216539 1106 48-49 1.2050720149493301 1106 50-51 0.18569800366199019 1106 52-53 0.6842194757737161 1106 54-55 0.5367158428039573 1106 56-57 0.6615368454204358 1106 58-59 0.95779973514842 1106 60-61 1.2673474156477624 1106 62-63 0.730619266125494 1106 64-65 -0.8245515961057492 1106 66-67 0.2885635185561952 1106 68-69 1.6684228910020238 1106 70-71 0.1022911033273175 1106 72-73 0.3864265146442918 1106 74-75 -0.7722904913084392 1106 76 -0.4437876301322454 1107 1 0.7474949471362748 1107 2 -0.3930235646844693 1107 3 0.1450230799110983 1107 4 0.24311347161186347 1107 5 0.01435581614254744 1107 6 0.23801490855473872 1107 7 0.7192521607258939 1107 8 0.13979815366035808 1107 9 -0.216292515242138 1107 10-11 0.7373781163609081 1107 12-13 0.16332053494127052 1107 14-15 -0.18642166615146039 1107 16-17 0.6134753934207353 1107 18-19 0.5988442483031235 1107 20-21 0.7040025009688193 1107 22-23 0.21757663219350576 1107 24-25 0.5439356965393713 1107 26-27 -0.06448984207352737 1107 28-29 0.9113161733038311 1107 30-31 0.8900307273481474 1107 32-33 1.3341589013608157 1107 34-35 -0.09535969772755237 1107 36-37 1.2466786065761255 1107 38-39 0.656494618780215 1107 40-41 0.16421831407894416 1107 42-43 0.5688252201654365 1107 44-45 0.41458686966264224 1107 46-47 0.8451547793135106 1107 48-49 0.6669875709904005 1107 50-51 0.9729631336598032 1107 52-53 0.8935815033026699 1107 54-55 0.8356152310495375 1107 56-57 1.347125112844509 1107 58-59 0.7023922630050947 1107 60-61 1.2015155388725844 1107 62-63 0.8904947304033968 1107 64-65 0.7324999660528704 1107 66-67 -0.037605369361656926 1107 68-69 1.264555729105588 1107 70-71 -0.1629617915820134 1107 72-73 1.047763088819515 1107 74-75 -1.260096368520589 1107 76 -0.15443324953360005 1108 1 -0.40745506063453973 1108 2 0.35232689897402736 1108 3 0.08814073001730094 1108 4 0.3957840409534157 1108 5 -0.12642540957366322 1108 6 -0.063207699330043 1108 7 -0.2457532788430825 1108 8 -0.3073603191158938 1108 9 -0.2176394561321544 1108 10-11 0.5124001336639168 1108 12-13 1.0277457538191683 1108 14-15 0.4062064227466422 1108 16-17 -0.2971991131326419 1108 18-19 0.12122418924494838 1108 20-21 0.7147132713153965 1108 22-23 -0.19155666314918562 1108 24-25 1.2414568072475518 1108 26-27 -0.3654145687999417 1108 28-29 -0.9634053725698664 1108 30-31 0.057271570740368816 1108 32-33 0.6839231867050586 1108 34-35 0.6035601592894864 1108 36-37 1.2709623966605719 1108 38-39 1.3131671567276797 1108 40-41 0.9841038241032933 1108 42-43 -0.5178581969199882 1108 44-45 0.4639833365330759 1108 46-47 0.8431602706879033 1108 48-49 0.7829799037884158 1108 50-51 0.9113535392962362 1108 52-53 0.17704780387769858 1108 54-55 0.05640992617464491 1108 56-57 0.07539273963827497 1108 58-59 0.32265854285797246 1108 60-61 0.9929469225953227 1108 62-63 0.7643485355114166 1108 64-65 0.1749441457140577 1108 66-67 0.8851009457189036 1108 68-69 1.207530914309963 1108 70-71 -0.8497203265247677 1108 72-73 -0.45599280404687903 1108 74-75 -0.4941921049346192 1108 76 0.3348430287651105 1109 1 -1.7499924900496566 1109 2 0.2702930182300989 1109 3 0.33597785378044875 1109 4 0.3486411098028199 1109 5 -0.176598957726803 1109 6 -0.4604775537568173 1109 7 -0.12496894480174348 1109 8 0.3910544350673959 1109 9 0.391747685762887 1109 10-11 0.31024243796894524 1109 12-13 0.2889486756447823 1109 14-15 0.6879501931450207 1109 16-17 0.8396060466870736 1109 18-19 0.5159296754387981 1109 20-21 0.7316406919235945 1109 22-23 0.4236067829472745 1109 24-25 -0.37063716778224176 1109 26-27 0.24204282124306076 1109 28-29 -0.8525029221735565 1109 30-31 0.5860106268456349 1109 32-33 0.9246111626673397 1109 34-35 -0.15817376807930827 1109 36-37 1.334618305068588 1109 38-39 1.1037307996847332 1109 40-41 0.5561781130739192 1109 42-43 0.9683227076026242 1109 44-45 0.8216220455420427 1109 46-47 0.7697778948913978 1109 48-49 0.5890981237542263 1109 50-51 0.04331489245377185 1109 52-53 -0.4908406072500995 1109 54-55 -0.3980531106590135 1109 56-57 -0.029759309266040646 1109 58-59 -0.24233135508535497 1109 60-61 0.9502592574655466 1109 62-63 0.25732890125767227 1109 64-65 0.4284798655503508 1109 66-67 0.4623946306383431 1109 68-69 0.10626427181915687 1109 70-71 0.6385457461064306 1109 72-73 0.3995218827893652 1109 74-75 0.22734081740905765 1109 76 0.930993886144794 1110 1 -0.36172134710086823 1110 2 -0.40304708654911536 1110 3 0.041419957918158445 1110 4 0.104601764138323 1110 5 0.3436886505272341 1110 6 0.055239328526944576 1110 7 0.6242291734490806 1110 8 0.49380221652788947 1110 9 0.7412879402266981 1110 10-11 -0.5558165805277966 1110 12-13 0.7714943829044927 1110 14-15 1.0488638037512459 1110 16-17 0.6417951838623068 1110 18-19 -0.3115187368353318 1110 20-21 0.7453395051386025 1110 22-23 -0.5357110829780964 1110 24-25 -0.650734462767808 1110 26-27 1.0236235974645922 1110 28-29 0.31700151490545636 1110 30-31 0.32714983351729643 1110 32-33 1.1712833534082847 1110 34-35 0.9009115521388011 1110 36-37 1.1448930832146402 1110 38-39 1.1967490825886102 1110 40-41 -0.6260628791211005 1110 42-43 0.0034183988246923036 1110 44-45 0.7017273593450994 1110 46-47 0.42360554377410864 1110 48-49 1.3537191009807827 1110 50-51 -0.12009524333154076 1110 52-53 0.5993443601400372 1110 54-55 -0.2014317057621824 1110 56-57 0.8174688528209941 1110 58-59 -0.02936492720125017 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2116 44-45 -0.37989659739641013 2116 46-47 -0.8653035898805328 2116 48-49 0.04991047279222727 2116 50-51 -1.2653138868016214 2116 52-53 -0.014855710390108356 2116 54-55 -0.1140918679815357 2116 56-57 -0.08424444211259186 2116 58-59 -2.0943261356178553 2116 60-61 -1.1761878954259828 2116 62-63 0.46176267232294066 2116 64-65 -2.0404115528677025 2116 66-67 -0.600488847525952 2116 68-69 -1.007411843564391 2116 70-71 1.2766395299427415 2116 72-73 0.6133812347980268 2116 74-75 0.8476800827967708 2116 76 -0.13172301326940783 2117 1 0.9249183771354339 2117 2 0.5477136408965038 2117 3 0.524125294896642 2117 4 0.09491437557301197 2117 5 0.3816953068923823 2117 6 0.3064576810765658 2117 7 0.6866028808067455 2117 8 0.07278712084264072 2117 9 -1.021014448932938 2117 10-11 -0.24911861309643513 2117 12-13 -1.1534729295880837 2117 14-15 0.1173738477307964 2117 16-17 -0.32889006515706143 2117 18-19 -0.8683801418038399 2117 20-21 0.010212321018791215 2117 22-23 0.19530044679724767 2117 24-25 -1.0299392648597987 2117 26-27 -1.341124411569048 2117 28-29 -1.1014431540592398 2117 30-31 -0.08606483425876377 2117 32-33 -1.6439579398283755 2117 34-35 0.8088962187262254 2117 36-37 -0.39106317495723886 2117 38-39 0.654136459267562 2117 40-41 -0.24789813408366257 2117 42-43 0.25169487570570936 2117 44-45 -0.12545973206105998 2117 46-47 -0.5936594033315643 2117 48-49 -0.4865875672707318 2117 50-51 0.3036388586534855 2117 52-53 0.7397817784212748 2117 54-55 -0.38876083924608196 2117 56-57 0.7501177295593706 2117 58-59 -0.8907550377444622 2117 60-61 -0.2110500825305195 2117 62-63 0.26262072349737053 2117 64-65 -0.558915210387994 2117 66-67 -0.5095601151576687 2117 68-69 0.6961129565837609 2117 70-71 0.5134369619622419 2117 72-73 1.3359498325942525 2117 74-75 0.7501925060197152 2117 76 0.8605298340406939 2118 1 0.11654449595563676 2118 2 0.2088838217098683 2118 3 -0.625361099048213 2118 4 0.19153839191228883 2118 5 0.19172113737235463 2118 6 0.21469940631234863 2118 7 0.8442589149270034 2118 8 0.535783456518736 2118 9 -1.3500527140858196 2118 10-11 -0.7076697033829831 2118 12-13 -1.2387435489188903 2118 14-15 0.46853321578510787 2118 16-17 -2.743213967361662 2118 18-19 -0.24691681507830054 2118 20-21 -0.09974743148917042 2118 22-23 0.4306581689093463 2118 24-25 0.4844176763307999 2118 26-27 -2.5606534558412903 2118 28-29 -0.7145416102375322 2118 30-31 -0.5091290500333798 2118 32-33 -0.3689534345121004 2118 34-35 0.2596681295810299 2118 36-37 -3.0127367497755273 2118 38-39 -0.7998192084203097 2118 40-41 -0.4726082920700847 2118 42-43 0.2778407278111956 2118 44-45 -2.26221109867366 2118 46-47 -0.09681312472280723 2118 48-49 0.34874312294371634 2118 50-51 -0.8532512717003371 2118 52-53 -1.6301797762131969 2118 54-55 -0.8322942086219456 2118 56-57 0.32905087066587413 2118 58-59 -0.803186707114314 2118 60-61 -0.7764522570965084 2118 62-63 -1.0886888217660484 2118 64-65 -0.13702237684441343 2118 66-67 0.6807444442223982 2118 68-69 -1.0911610267165344 2118 70-71 -0.3710317110719359 2118 72-73 0.9381153879495727 2118 74-75 0.5217429355265253 2118 76 0.21756113097107388 2119 1 0.6388451894722138 2119 2 -1.3540416729774165 2119 3 0.001762397802330895 2119 4 -0.21423242949128962 2119 5 -0.07362848405667677 2119 6 -0.31596802385880807 2119 7 -0.6610219910983162 2119 8 0.2788960098049884 2119 9 0.08803979720768496 2119 10-11 -0.02815071120890167 2119 12-13 0.10232026733103083 2119 14-15 0.09061440176207469 2119 16-17 0.030486484378492662 2119 18-19 -0.07541462000290267 2119 20-21 -0.7695635541588928 2119 22-23 0.18662304770300864 2119 24-25 0.8126609797602029 2119 26-27 0.32736588627234653 2119 28-29 0.21500919462344825 2119 30-31 0.9945771279904108 2119 32-33 0.8112336240868672 2119 34-35 0.8190942287093606 2119 36-37 0.3281361903529998 2119 38-39 -1.4863051790253863 2119 40-41 -0.5497063387711094 2119 42-43 0.12563292734036224 2119 44-45 0.3110217065690648 2119 46-47 0.3047902346969309 2119 48-49 -0.5991954149451928 2119 50-51 0.5545396553213635 2119 52-53 0.1566275025295667 2119 54-55 0.388899403390532 2119 56-57 -1.681256439889573 2119 58-59 -0.11423525327452921 2119 60-61 -0.12259019690690565 2119 62-63 0.17845821647279791 2119 64-65 -0.13433420480140512 2119 66-67 0.6755907889446746 2119 68-69 0.5315564129984196 2119 70-71 0.6661096008832743 2119 72-73 1.2615390693136987 2119 74-75 1.6334690919176964 2119 76 1.103067270819647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 8.0 23 21.0 24 46.0 25 122.0 26 205.0 27 408.0 28 665.0 29 1058.0 30 1616.0 31 2392.0 32 3595.0 33 5123.0 34 7660.0 35 9630.0 36 13385.0 37 23853.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.411376988107182 23.590772316950854 10.937097005301618 34.06075368964035 2 15.586760280842526 17.306204327267515 46.71013039117352 20.396905000716433 3 14.469121650666283 27.407938100014327 37.54979223384439 20.573148015474995 4 10.253617996847685 24.480584610975782 36.22725318813584 29.038544204040694 5 9.590199168935378 40.366814729904 38.02693795672732 12.0160481444333 6 20.243587906576874 41.26952285427712 17.079810861154893 21.407078377991116 7 20.11176386301762 36.493767015331706 19.98280555953575 23.411663562114914 8 18.10861154893251 37.40220661985958 23.365811720876916 21.123370110330995 9 18.833643788508382 18.55423413096432 18.38372259636051 44.228399484166786 10-11 19.22911591918613 25.309499928356498 31.227253188135833 24.234130964321533 12-13 22.026078234704112 18.987677317667288 28.332855709987104 30.653388737641496 14-15 22.084109471270956 22.829918326407793 21.229402493193867 33.856569709127385 16-17 24.473420260782348 27.94741366958017 20.435592491761 27.143573577876484 18-19 22.897263218226108 25.980799541481588 21.243014758561397 29.878922481730907 20-21 21.02665138271959 23.8945407651526 20.929932655108182 34.14887519701963 22-23 22.818455366098295 30.317380713569282 21.17996847685915 25.68419544347328 24-25 24.36882074795816 31.91359793666714 20.96360510101734 22.75397621435736 26-27 25.49147442326981 29.351626307493913 21.36194297177246 23.79495629746382 28-29 23.559965611119072 22.669436882074795 21.811864163920333 31.958733342885797 30-31 27.883650952858574 24.959879638916753 21.111907150021494 26.04456225820318 32-33 28.228256197162914 27.334861728041265 21.574007737498206 22.862874337297605 34-35 19.545063762716723 32.31623441753833 24.790800974351626 23.347900845393323 36-37 17.549075798825047 32.673019057171516 26.697234560825333 23.080670583178104 38-39 20.243587906576874 24.16606963748388 28.39733486172804 27.193007594211206 40-41 19.104456225820318 24.066485169795097 31.236566843387305 25.592491760997277 42-43 25.366814729903997 23.090700673448918 32.078377991116206 19.46410660553088 44-45 19.23269809428285 25.53087834933371 29.74423269809428 25.492190858289156 46-47 25.161197879352343 21.055308783493338 27.442326980942827 26.34116635621149 48-49 22.678750537326266 20.8303481874194 28.27840664851698 28.212494626737357 50-51 17.49176099727755 20.89124516406362 33.17810574580886 28.43888809284998 52-53 19.14099441180685 21.19143143716865 37.76758847972489 21.899985671299614 54-55 17.527582748244736 21.214357357787648 34.126665711419975 27.13139418254764 56-57 24.162487462387162 20.895543774179682 29.468405215646943 25.473563547786217 58-59 18.420977217366385 28.15016478005445 32.922338443903136 20.50651955867603 60-61 20.77446625591059 36.323971915747244 23.663848688923913 19.237713139418254 62-63 15.87978220375412 42.604241295314516 21.88422410087405 19.631752400057316 64-65 15.454219802263935 43.78994125232841 21.090414099441183 19.66542484596647 66-67 15.35965037971056 43.233987677317664 20.900558819315087 20.505803123656683 68-69 15.670583178105746 40.745092420117494 21.11119071500215 22.47313368677461 70-71 16.399914027797678 38.31924344461957 21.54176816162774 23.739074365955005 72-73 16.644218369393897 35.07809141710847 22.102736781773892 26.17495343172374 74-75 17.0962888665998 34.180398337870756 22.129961312508957 26.593351483020488 76 17.011033099297894 35.15833213927497 21.49591632038974 26.334718441037396 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.0 14 3.5 15 3.5 16 1.5 17 2.0 18 3.5 19 5.5 20 6.5 21 7.0 22 18.5 23 44.0 24 57.0 25 56.0 26 89.0 27 145.5 28 221.0 29 273.0 30 341.5 31 464.0 32 690.0 33 862.0 34 978.5 35 1252.5 36 1547.0 37 1684.0 38 1902.5 39 2472.0 40 3093.0 41 3750.0 42 4137.0 43 5181.5 44 7126.5 45 7936.0 46 7845.0 47 7330.0 48 5989.0 49 4583.5 50 4004.0 51 3633.0 52 2794.0 53 2175.0 54 2024.0 55 1849.5 56 1409.0 57 1016.5 58 890.0 59 683.0 60 411.5 61 278.5 62 210.0 63 156.0 64 77.5 65 38.5 66 18.0 67 12.0 68 7.5 69 2.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 69790.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.21306777475283 #Duplication Level Percentage of deduplicated Percentage of total 1 60.317276962766165 22.445909156039548 2 16.325901967579224 12.150737928069924 3 8.521042701474721 9.512824186846252 4 4.797658927265027 7.141424272818456 5 2.6491086211543644 4.929072933084969 6 1.7558045512302185 3.9203324258489753 7 1.3245543105771822 3.450351053159478 8 0.8432482384197758 2.5103883077804845 9 0.6622771552885911 2.2180828198882363 >10 2.7145662469677716 14.91760997277547 >50 0.042354934349851756 1.1563261212208054 >100 0.03080358861807401 2.36996704398911 >500 0.003850448577259251 0.8539905430577447 >1k 0.011551345731777752 12.422983235420547 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGT 3988 5.714285714285714 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCTT 3500 5.015045135406218 RNA PCR Primer, Index 15 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 1182 1.6936523857286143 No Hit CTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 596 0.8539905430577447 No Hit TCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 436 0.6247313368677461 No Hit TATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTCT 301 0.43129388164493476 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 197 0.2822753976214358 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 187 0.2679466972345608 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGT 167 0.239289296460811 No Hit CTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 143 0.2049004155323112 No Hit TCTTAATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCC 112 0.160481444332999 No Hit CTTATAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 111 0.1590485742943115 No Hit CTTAACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 95 0.13612265367531165 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAATATCGTATGCCGTCTT 94 0.13468978363662415 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGT 86 0.12322682332712423 No Hit TTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCGTC 80 0.11462960309499928 No Hit CTTATACACATCTCCGAGCCCACGAGACCGCGGACAACCTCGTATGCCGT 78 0.1117638630176243 No Hit ATACACATCTCCGAGCCCACGAGACCGCGGACAAACTCGTATGCCGTCTT 78 0.1117638630176243 No Hit CTTTATACACATCTCCGAGCCCACGAGACCGCGGACAATCTCGTATGCCG 70 0.10030090270812438 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.012895830348187419 0.0 2 0.0 0.0 0.0 0.012895830348187419 0.0 3 0.0 0.0 0.0 0.012895830348187419 0.0 4 0.0 0.0 0.0 0.012895830348187419 0.0 5 0.0 0.0 0.0 0.01432870038687491 0.0 6 0.0 0.0 0.0 0.01432870038687491 0.0 7 0.0 0.0 0.0 0.01432870038687491 0.0 8 0.0 0.0 0.0 0.01432870038687491 0.0 9 0.0 0.0 0.0 0.017194440464249894 0.0 10 0.0 0.0 0.0 0.017194440464249894 0.0 11 0.0 0.0 0.0 0.017194440464249894 0.0 12 0.0 0.0 0.0 0.020060180541624874 0.0 13 0.0 0.0 0.0 0.020060180541624874 0.0 14 0.0 0.0 0.0 0.020060180541624874 0.0 15 0.0 0.0 0.0 0.021493050580312367 0.0 16 0.0 0.0 0.0 0.024358790657687347 0.0 17 0.0 0.0 0.0 0.025791660696374837 0.0 18 0.0 0.0 0.0 0.02865740077374982 0.0 19 0.0 0.0 0.0 0.03725462100587477 0.0 20 0.0 0.0 0.0 0.04155323112193724 0.0 21 0.0 0.0 0.0 0.0472847112766872 0.0 22 0.0 0.0 0.0 0.05015045135406219 0.0 23 0.0 0.0 0.0 0.05301619143143717 0.0 24 0.0 0.0 0.0 0.05301619143143717 0.0 25 0.0 0.0 0.0 0.06018054162487462 0.0 26 0.0 0.0 0.0 0.061613411663562115 0.0 27 0.0 0.0 0.0 0.07307637197306205 0.0 28 0.0 0.0 0.0 0.09027081243731194 0.0 29 0.0 0.0 0.0 0.10603238286287434 0.0 30 0.0 0.0 0.0 0.1260925634044992 0.0 31 0.0 0.0 0.0 0.16764579452643646 0.0 32 0.0 0.0 0.0 0.21063189568706117 0.0 33 0.0 0.0 0.0 0.2693795672732483 0.0 34 0.0 0.0 0.0 0.31523140851124803 0.0 35 0.0 0.0 0.0 0.41696518125805987 0.0 36 0.0 0.0 0.0 0.5244304341596218 0.0 37 0.0 0.0 0.0 0.7723169508525577 0.0 38 0.0 0.0 0.0 0.9987104169651813 0.0 39 0.0 0.0 0.0 1.3096432153603668 0.0 40 0.0 0.0 0.0 1.7194440464249892 0.0 41 0.0 0.0 0.0 2.124946267373549 0.0 42 0.0 0.0 0.0 2.5605387591345465 0.0 43 0.0 0.0 0.0 2.938816449348044 0.0 44 0.0 0.0 0.0 3.3629459807995414 0.0 45 0.0 0.0 0.0 3.8515546639919758 0.0 46 0.0 0.0 0.0 4.271385585327411 0.0 47 0.0 0.0 0.0 4.75712852844247 0.0 48 0.0 0.0 0.0 5.209915460667718 0.0 49 0.0 0.0 0.0 5.761570425562401 0.0 50 0.0 0.0 0.0 6.134116635621149 0.0 51 0.0 0.0 0.0 6.592635048001147 0.0 52 0.0 0.0 0.0 7.051153460381143 0.0 53 0.0 0.0 0.0 7.483880212064766 0.0 54 0.0 0.0 0.0 7.962458804986388 0.0 55 0.0 0.0 0.0 8.353632325548073 0.0 56 0.0 0.0 0.0 8.813583607966757 0.0 57 0.0 0.0 0.0 9.249176099727755 0.0 58 0.0 0.0 0.0 9.610259349477003 0.0 59 0.0 0.0 0.0 9.955581028800689 0.0 60 0.0 0.0 0.0 10.30233557816306 0.0 61 0.0 0.0 0.0 10.666284567989683 0.0 62 0.0 0.0 0.0 11.116205760137555 0.0 63 0.0 0.0 0.0 11.523140851124802 0.0 64 0.0 0.0 0.0 11.973062043272675 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATGGA 15 0.0022161293 70.0 35 ATATGGA 20 7.876604E-5 70.0 4 ATATATG 20 7.876604E-5 70.0 2 CTTGAAC 25 2.3754459E-4 55.999996 54 ATGCAGT 25 2.3754459E-4 55.999996 44 AGTTTTC 20 0.006924829 52.5 68 CTATAAC 20 0.006924829 52.5 4 GTGGATG 20 0.006924829 52.5 12 TCGTGCC 20 0.006924829 52.5 9 GCGGACC 20 0.006924829 52.5 27 GACTATC 20 0.006924829 52.5 38 GACTACT 20 0.006924829 52.5 44 TTTACAC 210 0.0 51.666664 2 TTAATAC 55 2.233719E-9 50.90909 3 CTTATAA 35 2.0671803E-5 50.0 1 GAACAAA 35 2.0671803E-5 50.0 57 CTTTACA 225 0.0 49.77778 1 TTACACA 225 0.0 49.77778 3 CTATACA 110 0.0 47.727272 1 CGTCTGC 45 1.6629583E-6 46.666664 38 >>END_MODULE