FastQCFastQC Report
Wed 25 May 2016
SRR1780443_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780443_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419804
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACA301.3072486E-436.6305352
ACGGGTA250.002345924535.2156538
CTGTAGC551.3676254E-732.006591
CGCGGTA454.090712E-529.30442843
CCGTGCG407.065028E-427.4729029
CGGTGGT1600.026.09925743
CTATACA1350.026.0794451
GATCCTA701.127295E-625.1480371
CCGGCCA450.001383424224.4845221
AGATACC1001.7516868E-924.18191127
CTTAGGA551.599291E-423.98206327
GATTTCG651.8235962E-523.66895941
TCGGTGG2800.023.54819942
GCGTGGA752.0509742E-623.477134
TCTCGCG752.0552343E-623.471511
TGACGGT602.8483142E-422.02291713
TTTAACG500.002571008622.0097834
AGTGCGT500.002579927421.9966623
GATACCC602.8829908E-421.98355928
AATTTGC1004.6424248E-821.98355729
Produced by FastQC (version 0.11.5)