FastQCFastQC Report
Wed 25 May 2016
SRR1780443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780443_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419804
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGT309867.381063543939553No Hit
ATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCTT230665.494468847366867RNA PCR Primer, Index 45 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC71661.7069870701565495No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG39300.9361511562538708No Hit
CTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC37910.9030404665034159No Hit
TATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTCT21460.5111909367228517No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC18310.43615592038189255No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC14960.35635677601928517No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG10670.2541662299549313No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCC8850.21081266495793274No Hit
CTTATAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG7370.1755581175977361No Hit
CTTAACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC6310.15030823908300064No Hit
TTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCGTC5420.12910786938666616No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCTTATTAATCTCGTATGCCG4430.10552543567950758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATTA207.8044966E-444.0528341
ACGTTCC207.8550575E-443.99511717
GCGCCGT207.8550575E-443.99511732
CCGTCTT25300.042.087344
CTTTACA9300.039.5528141
TTTACAC9450.038.897162
CGCAAAG404.1197927E-738.49572812
GCCGTCT27800.038.2233343
CTATACA4200.036.7106931
GTAGGTC250.002352330135.1960934
CCACGTT250.002352330135.1960937
AGTAACC250.002352330135.1960932
CAAAGGC505.93609E-835.1960914
AAGCAGT451.0359727E-634.21842630
TTACACA11000.033.616293
CTTTATA2500.033.4562152
CTTATAC46100.031.104551
CCCAACG502.358649E-630.79658121
TTATACA46350.030.7247072
TCTTTAT2800.029.8929941
Produced by FastQC (version 0.11.5)