FastQCFastQC Report
Wed 25 May 2016
SRR1780442_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780442_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15483
Sequences flagged as poor quality0
Sequence length76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC260.16792611251049538No Hit
CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG180.11625653943034295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCC150.002185882369.9805811
TTACGCC150.002185882369.9805810
TTTACAC409.798896E-961.233012
CTTTACA307.1243594E-659.4719471
CACAACG200.006831068552.48543568
CCTGACG200.006831068552.4854358
AGGCACA200.006831068552.4854352
TTACACA505.6976205E-848.9864043
ACAGACA305.7079917E-446.6537246
CACCTGA305.7079917E-446.653726
ACACCTG350.001219380339.9889035
ACCTGAC350.001219380339.9889037
GATCTCG350.001219380339.98890340
AGATCTC350.001219380339.98890339
TAGATCT350.001219380339.98890338
ATCTCGG400.002349753634.9902941
TCTCGGT400.002349753634.9902942
TGTAGAT501.6364703E-434.9902934
GCCGACG601.1430469E-534.9902919
CTGACGC1001.1019438E-831.491269
Produced by FastQC (version 0.11.5)