##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780442_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15483 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.153910740812503 21.0 12.0 23.0 12.0 32.0 2 18.348317509526577 12.0 11.0 23.0 11.0 32.0 3 18.205063618161855 11.0 11.0 23.0 11.0 32.0 4 18.265194083833883 11.0 11.0 23.0 11.0 32.0 5 18.8642381967319 11.0 11.0 23.0 11.0 33.0 6 20.845249628624945 21.0 11.0 30.0 11.0 34.0 7 21.551766453529677 21.0 11.0 31.0 11.0 36.0 8 21.80365562229542 21.0 11.0 31.0 11.0 36.0 9 22.171090873861655 21.0 12.0 33.0 12.0 36.0 10-11 20.65810889362527 21.0 11.0 31.0 11.0 35.0 12-13 20.535845766324357 21.0 11.0 29.5 11.0 34.0 14-15 20.528127623845506 21.0 11.0 29.5 11.0 34.0 16-17 19.97946134470064 21.0 11.0 27.5 11.0 34.0 18-19 20.194439062197247 21.0 11.0 28.0 11.0 34.0 20-21 20.193760899050574 16.0 11.0 29.0 11.0 34.0 22-23 19.763611703158304 16.0 11.0 27.5 11.0 34.0 24-25 19.808628818704385 16.0 11.0 27.0 10.5 34.0 26-27 19.35878059807531 11.5 11.0 26.0 10.5 34.0 28-29 18.94474585028741 11.0 11.0 26.0 11.0 34.0 30-31 19.019246915972357 11.0 11.0 26.0 11.0 34.0 32-33 19.152199186204225 11.0 11.0 26.0 11.0 34.0 34-35 19.405347800813793 16.0 11.0 26.5 11.0 34.0 36-37 19.227862817283473 11.0 11.0 26.0 11.0 34.0 38-39 18.64819479429051 11.0 11.0 26.0 11.0 34.0 40-41 19.05476974746496 11.0 11.0 26.0 11.0 34.0 42-43 19.122230833817735 11.0 11.0 26.5 11.0 34.0 44-45 19.0168571982174 11.0 11.0 26.0 11.0 34.0 46-47 19.119130659432926 11.0 11.0 26.0 11.0 34.0 48-49 19.316476135115934 11.0 11.0 26.0 11.0 34.0 50-51 19.391106374733578 20.0 11.0 26.0 11.0 34.0 52-53 19.529936058903314 20.0 11.0 26.0 11.0 34.0 54-55 19.503197054834335 20.5 11.0 26.0 11.0 34.0 56-57 18.82703610411419 11.0 11.0 26.0 11.0 33.5 58-59 19.023154427436545 16.0 11.0 26.0 11.0 33.5 60-61 19.225150164696764 19.0 11.0 26.0 11.0 33.0 62-63 18.878092101014015 15.0 11.0 26.0 11.0 33.0 64-65 19.089840470193117 15.0 11.0 26.0 11.0 33.0 66-67 19.46347607052897 19.0 11.0 26.0 11.0 33.0 68-69 18.828295549958018 15.0 11.0 25.5 11.0 33.0 70-71 18.883840341019184 15.0 11.0 25.5 11.0 33.0 72-73 18.4658980817671 11.0 11.0 25.0 11.0 32.0 74-75 18.157366143512238 11.0 11.0 25.0 11.0 32.0 76 17.460117548278756 11.0 11.0 24.0 11.0 31.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04157864927639565 1101 2 -0.9955745387713719 1101 3 0.42971271193630045 1101 4 0.28782685500328853 1101 5 -1.6263002234734252 1101 6 -0.13614088195577878 1101 7 0.7003289645848483 1101 8 -1.563143737532453 1101 9 -0.3150915983325824 1101 10-11 -0.19410091167253896 1101 12-13 1.4657480191969832 1101 14-15 -0.19188861637675814 1101 16-17 0.604159209899013 1101 18-19 -2.463821265322121 1101 20-21 -0.8082777030479349 1101 22-23 -0.2710182916782955 1101 24-25 0.4123931513883896 1101 26-27 -1.5365542174770077 1101 28-29 0.933905846553813 1101 30-31 1.3207998046768132 1101 32-33 0.30760556838224673 1101 34-35 0.07537670084045445 1101 36-37 -0.4853761717489711 1101 38-39 -0.31489478427208795 1101 40-41 -2.9337476423683597 1101 42-43 -0.4233363157138861 1101 44-45 -2.4720942799025316 1101 46-47 -0.5271261303772441 1101 48-49 -0.2844445160301241 1101 50-51 -0.3219556707301976 1101 52-53 -0.1408882342836506 1101 54-55 -1.5949694795225184 1101 56-57 -0.6361676199782558 1101 58-59 -2.0767858905440377 1101 60-61 -0.20494270084412847 1101 62-63 0.16676347205599384 1101 64-65 -1.7800095631820625 1101 66-67 -1.1029024777798426 1101 68-69 1.4287322604887365 1101 70-71 0.660799750424502 1101 72-73 -0.7011845557744927 1101 74-75 1.9210158409571356 1101 76 0.2637346302168808 1102 1 -0.6745978213118384 1102 2 -1.8735157152419575 1102 3 0.6341244766421852 1102 4 -3.0165849097025923 1102 5 -3.2807119881793074 1102 6 -1.1229055878381295 1102 7 -4.464376917768092 1102 8 -0.824908443414806 1102 9 0.9260848722556538 1102 10-11 -1.5124832646137172 1102 12-13 1.7679539015499266 1102 14-15 0.032376089505596894 1102 16-17 -0.928929025395103 1102 18-19 -0.34911538296917755 1102 20-21 -0.1979835854008769 1102 22-23 2.2628052377334704 1102 24-25 0.5998931513883896 1102 26-27 -0.7203777468887722 1102 28-29 -1.760947094622658 1102 30-31 -2.9630237247349527 1102 32-33 -4.297541490441279 1102 34-35 -2.1400644756301297 1102 36-37 -2.3493467599842646 1102 38-39 -0.9656300783897329 1102 40-41 0.3853700046904649 1102 42-43 -0.9851010215962397 1102 44-45 -0.1632707504907671 1102 46-47 -1.2521261303772455 1102 48-49 0.4302613663228172 1102 50-51 -1.5829850824949006 1102 52-53 2.276758824539879 1102 54-55 -1.325851832463691 1102 56-57 -1.3957264435076695 1102 58-59 -0.6356094199558022 1102 60-61 -1.167442700844127 1102 62-63 1.1785281779383503 1102 64-65 1.9846963191708795 1102 66-67 -2.1580495366033716 1102 68-69 0.03755578990050168 1102 70-71 -0.9465531907519704 1102 72-73 -1.238684555774494 1102 74-75 -0.758395923748747 1102 76 -1.4730300756654717 1103 1 -0.9725517855062122 1103 2 0.4794254612286295 1103 3 -2.025722070672394 1103 4 0.46934859413372365 1103 5 1.3139171678309225 1103 6 1.3323373789137882 1103 7 -0.351844948458627 1103 8 -0.5870567810107161 1103 9 0.3109953581891567 1103 10-11 -0.8826878681942816 1103 12-13 -1.8010998068899688 1103 14-15 1.3814809488406325 1103 16-17 0.22644181859466173 1103 18-19 1.9840048216344037 1103 20-21 1.2515049056477174 1103 22-23 1.5844164909304013 1103 24-25 -0.32401989208987203 1103 26-27 0.19714143469690626 1103 28-29 0.36053628133642235 1103 30-31 1.5631911090246398 1103 32-33 -1.4625031272699225 1103 34-35 0.973746266057848 1103 36-37 2.4140803499901615 1103 38-39 -0.8121773929677403 1103 40-41 -0.4413563380205332 1103 42-43 0.8614462929817677 1103 44-45 -0.21122471468514092 1103 46-47 -1.8608217825511595 1103 48-49 -1.2474879942909922 1103 50-51 -1.3464121924693302 1103 52-53 0.19172046136852217 1103 54-55 -1.0330129577833844 1103 56-57 -2.129102402586952 1103 58-59 -0.9680902383701238 1103 60-61 -0.06961661388760376 1103 62-63 -2.515845223596177 1103 64-65 -1.616966084921195 1103 66-67 0.6117714352636341 1103 68-69 -2.358224261250392 1103 70-71 -2.129417640879847 1103 72-73 -2.8908584688179726 1103 74-75 -0.3376798112167769 1103 76 -0.6917001523918138 1104 1 -1.1721968609276843 1104 2 -0.5001663755060655 1104 3 0.2577739364260978 1104 4 -0.4463568184660964 1104 5 0.7384956948939205 1104 6 0.6868183017176896 1104 7 1.53502284213587 1104 8 -0.5049804722263325 1104 9 3.1277655445245607 1104 10-11 -0.5514988708562143 1104 12-13 2.9573296518500456 1104 14-15 2.4981624040314045 1104 16-17 1.5569653323479926 1104 18-19 0.35046444896359574 1104 20-21 1.0871304602173701 1104 22-23 1.6172470144441533 1104 24-25 1.2274441717965523 1104 26-27 -0.43196238074231275 1104 28-29 2.25916094859463 1104 30-31 1.9784528659012999 1104 32-33 0.9629627112393919 1104 34-35 2.156754251860864 1104 36-37 0.48528709355715094 1104 38-39 -1.1835172332516812 1104 40-41 -0.1281353974704018 1104 42-43 -0.8182342748975593 1104 44-45 0.01770163846481765 1104 46-47 1.3550167267656086 1104 48-49 0.9767799737657938 1104 50-51 0.4038096353922498 1104 52-53 0.776458704491862 1104 54-55 -0.21690825503272038 1104 56-57 0.7717405432870521 1104 58-59 -1.0461736456460784 1104 60-61 1.7560266869109746 1104 62-63 0.7718655128723206 1104 64-65 0.35774553885875093 1104 66-67 0.7657199711997507 1104 68-69 -0.6284105966541169 1104 70-71 0.6602895463428666 1104 72-73 0.15417258708264825 1104 74-75 2.046526045038771 1104 76 0.08159177307402388 1105 1 -0.07786579516804792 1105 2 -2.0761300943269276 1105 3 0.6047127119363012 1105 4 -1.5316175894411543 1105 5 -1.1068557790289795 1105 6 -1.4111408819557774 1105 7 -3.7552265909707074 1105 8 -3.2464770708657866 1105 9 -1.0150915983325817 1105 10-11 -0.2954898005614268 1105 12-13 1.3088035747525417 1105 14-15 -0.21272194971009384 1105 16-17 0.5472147654545694 1105 18-19 1.4250676235667719 1105 20-21 -1.1888332586034913 1105 22-23 -1.1237960694560698 1105 24-25 -2.450106848611611 1105 26-27 -0.46710977303256485 1105 28-29 -0.48414970900174126 1105 30-31 -1.6208668619898567 1105 32-33 -2.2243388760621947 1105 34-35 -1.7593455213817641 1105 36-37 0.13267938380658606 1105 38-39 -0.48850589538319866 1105 40-41 -1.6920809757016926 1105 42-43 -0.7177807601583304 1105 44-45 -1.172094279902531 1105 46-47 -0.9021261303772441 1105 48-49 -0.7733334049190148 1105 50-51 0.8516554403809131 1105 52-53 -1.0019993453947613 1105 54-55 0.34114163158859157 1105 56-57 0.6735546022439678 1105 58-59 -0.5878970016551506 1105 60-61 -0.795220478621907 1105 62-63 -1.9387920834995604 1105 64-65 -0.8966762298487296 1105 66-67 -1.8737358111131783 1105 68-69 -0.29348996173348496 1105 70-71 -1.5864224717977216 1105 72-73 -1.333129000218939 1105 74-75 -0.17342860348730582 1105 76 0.2720679635502137 1106 1 -1.0429451602474131 1106 2 0.40799688980005655 1106 3 1.199950807174396 1106 4 0.6318744740509104 1106 5 -3.3227287949019964 1106 6 -2.115902786717683 1106 7 -1.89014722589134 1106 8 -1.0321913565800749 1106 9 -0.39604397928496127 1106 10-11 -1.4137437688153938 1106 12-13 -0.6515138855649205 1106 14-15 -0.5698648068529479 1106 16-17 -1.7678645996247973 1106 18-19 -1.756678408179262 1106 20-21 -0.29042056019079254 1106 22-23 -0.2722087678687686 1106 24-25 -0.6858211343258951 1106 26-27 0.5527314968087076 1106 28-29 -0.45002272487475636 1106 30-31 -1.1899144810374729 1106 32-33 -0.025132526855845327 1106 34-35 0.6771624151261726 1106 36-37 -0.768114266987066 1106 38-39 -0.0797757366530405 1106 40-41 1.1745856909649746 1106 42-43 -0.9780982204757898 1106 44-45 0.5279057200974684 1106 46-47 -0.7521261303772455 1106 48-49 0.6655554839698752 1106 50-51 -0.3546937659682925 1106 52-53 -1.7575549009503142 1106 54-55 -0.08425519380823232 1106 56-57 0.5727609514503129 1106 58-59 -0.4220239857821326 1106 60-61 -0.9293474627488898 1106 62-63 -0.8760936708011506 1106 64-65 -2.3395333727058727 1106 66-67 -0.3618310492084156 1106 68-69 -0.6998391680826899 1106 70-71 0.4357997504245006 1106 72-73 0.05893449184455335 1106 74-75 1.616253936195232 1106 76 1.7720679635502137 1107 1 0.7469708061391422 1107 2 -0.08920198975176419 1107 3 0.5851048687990463 1107 4 1.0030229334346608 1107 5 -0.2807119881793092 1107 6 1.4947414709853994 1107 7 0.21209367046720118 1107 8 -1.9327515806697093 1107 9 1.7103985977458507 1107 10-11 -0.16934600971175584 1107 12-13 -0.04086962786184145 1107 14-15 -1.0264474399061712 1107 16-17 -1.5514780450029484 1107 18-19 -0.7363702849299649 1107 20-21 0.027506610677555443 1107 22-23 1.4735895514589608 1107 24-25 1.8008735435452543 1107 26-27 2.9413869589935793 1107 28-29 1.9694450622400872 1107 30-31 0.21834882428465363 1107 32-33 0.2514781174018559 1107 34-35 0.31581787731104427 1107 36-37 -0.0601310737097549 1107 38-39 -0.30876733329169426 1107 40-41 -1.0705123482507162 1107 42-43 0.11293819409003802 1107 44-45 1.1455527789209974 1107 46-47 -0.6982045617497938 1107 48-49 0.8861437192639912 1107 50-51 -1.1418086119066686 1107 52-53 -1.737947057813063 1107 54-55 -1.2621263422676137 1107 56-57 -1.1800401689978628 1107 58-59 0.4526258741618463 1107 60-61 -0.8978348577068722 1107 62-63 0.5069595504873696 1107 64-65 0.4503825936806827 1107 66-67 -0.6090299287602363 1107 68-69 -0.0653853865700853 1107 70-71 -0.45635711232059606 1107 72-73 0.11915858148040925 1107 74-75 0.11415309585909839 1107 76 -0.3161673305674313 1108 1 -0.4000880173902708 1108 2 -0.9253364435332756 1108 3 -0.3238587166351259 1108 4 0.22711256928900525 1108 5 1.4629854908122901 1108 6 -1.806378977193873 1108 7 1.3717575360134227 1108 8 2.2535229291342134 1108 9 -0.5150915983325817 1108 10-11 1.1338752788036501 1108 12-13 -1.0443710284220629 1108 14-15 0.27537328838514696 1108 16-17 0.07737349561329765 1108 18-19 -1.2685831700840247 1108 20-21 -0.6713729411431757 1108 22-23 -0.4388754345354364 1108 24-25 0.11179791329314881 1108 26-27 -0.9234589793817669 1108 28-29 -0.5690703439223768 1108 30-31 0.13151409039109652 1108 32-33 0.5701055683822496 1108 34-35 0.9271624151261726 1108 36-37 0.481885733012934 1108 38-39 1.1345099776326713 1108 40-41 0.15077616715544906 1108 42-43 -1.9542886966662643 1108 44-45 -0.1387609465691959 1108 46-47 -2.394983273234388 1108 48-49 0.15365072206511599 1108 50-51 -1.807074718349245 1108 52-53 0.3495879561925399 1108 54-55 0.3562209966679575 1108 56-57 0.0013323800217435178 1108 58-59 -0.9458335095916581 1108 60-61 0.046843013441588255 1108 62-63 -0.7213317660392455 1108 64-65 0.4699904368179375 1108 66-67 1.9834070460296829 1108 68-69 -0.580791549035073 1108 70-71 -0.5761050114802622 1108 72-73 -0.44106550815544665 1108 74-75 0.21149203143332684 1108 76 -1.8231701316878812 1109 1 0.2665786492763971 1109 2 0.04464285253297717 1109 3 1.9090605380232581 1109 4 -0.008912275431491423 1109 5 0.18348238522222715 1109 6 1.1366852050007452 1109 7 2.930763747193545 1109 8 0.8042475668153699 1109 9 -0.84117855485432 1109 10-11 0.5520947405013743 1109 12-13 1.938030627892637 1109 14-15 2.501046166231937 1109 16-17 -0.29529731184011965 1109 18-19 0.5274830825039665 1109 20-21 0.0775918621694558 1109 22-23 -1.9808009003739464 1109 24-25 -0.49793293556813367 1109 26-27 0.6101849129577737 1109 28-29 1.012710194379899 1109 30-31 0.3784085003289839 1109 32-33 -0.08206834466122714 1109 34-35 1.75635496171002 1109 36-37 1.327123828251029 1109 38-39 -0.16000347992426 1109 40-41 0.7325567054577249 1109 42-43 -0.09507544614866603 1109 44-45 1.506166589662687 1109 46-47 0.052221695709707916 1109 48-49 0.46990331005683217 1109 50-51 1.4253269379654547 1109 52-53 -1.9495838864575639 1109 54-55 1.6191609552600923 1109 56-57 0.7295932495869621 1109 58-59 0.18408367467335296 1109 60-61 1.691252951329787 1109 62-63 -0.48323652794400473 1109 64-65 -0.9974008675298904 1109 66-67 0.1552496961332004 1109 68-69 -0.6843112177721302 1109 70-71 0.6205823591201529 1109 72-73 2.0330545746602873 1109 74-75 -1.6855058981733002 1109 76 -1.0830045002179016 1110 1 -1.545921350723603 1110 2 -1.1664078721047026 1110 3 -1.8119539547303667 1110 4 -0.1955064783300422 1110 5 -0.022133556806757326 1110 6 -0.9194742152891102 1110 7 -0.03300436874848245 1110 8 0.3368562624675455 1110 9 0.17240840166741833 1110 10-11 1.0683990883274603 1110 12-13 1.4407480191969846 1110 14-15 0.6414447169565776 1110 16-17 0.2187425432323451 1110 18-19 0.7195120680112161 1110 20-21 1.545888963618733 1110 22-23 -1.0043516250116298 1110 24-25 -1.8167735152782782 1110 26-27 -0.4011375508103434 1110 28-29 0.058905846553813035 1110 30-31 2.720799804676812 1110 32-33 1.2471889017155853 1110 34-35 -0.12045663249287841 1110 36-37 0.8792071615843646 1110 38-39 -0.7003114509387522 1110 40-41 -0.11708097570169684 1110 42-43 1.1766636842861153 1110 44-45 -0.4408442799025316 1110 46-47 2.33120720295609 1110 48-49 -0.6781945160301248 1110 50-51 0.8447109959364703 1110 52-53 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1.3877777061920966 2116 50-51 1.323877662603138 2116 52-53 -0.27144378983920703 2116 54-55 1.005030520477483 2116 56-57 0.8902212689106328 2116 58-59 0.03432522056707654 2116 60-61 3.1797795213780944 2116 62-63 1.0723190276115524 2116 64-65 -0.5855651187376196 2116 66-67 1.167930855553493 2116 68-69 0.6315100382665157 2116 70-71 -0.022533582908835115 2116 72-73 1.9696487775588416 2116 74-75 3.09046028540158 2116 76 1.410956852439103 2117 1 0.22603810873585672 2117 2 1.7767227585259278 2117 3 0.7128208200444099 2117 4 0.16147550365193908 2117 5 0.7081592359860345 2117 6 0.2212915504766535 2117 7 0.9895181537740392 2117 8 1.7084778840891666 2117 9 0.47139488815390607 2117 10-11 -0.6579522630238905 2117 12-13 2.037031802980767 2117 14-15 -0.7172264542145967 2117 16-17 -1.2488813306415274 2117 18-19 -2.6340915355923897 2117 20-21 0.20185743208720197 2117 22-23 0.31884657318656906 2117 24-25 -2.163620362125126 2117 26-27 0.2641214581986695 2117 28-29 -0.4258914507434852 2117 30-31 -0.6136596547826478 2117 32-33 1.8876731359498145 2117 34-35 0.34125507921883624 2117 36-37 0.5636103147375131 2117 38-39 -0.6476650545423581 2117 40-41 -0.9560449396656594 2117 42-43 1.386123143745575 2117 44-45 -0.634256442064693 2117 46-47 0.7681441398930247 2117 48-49 -0.8006607322463424 2117 50-51 0.6324362211616936 2117 52-53 0.4901928467974308 2117 54-55 2.7111116015585637 2117 56-57 1.8188999475893084 2117 58-59 -0.5227318364899816 2117 60-61 -0.5931183765198043 2117 62-63 0.43568239097491457 2117 64-65 -0.030009563182062493 2117 66-67 0.1893272519498872 2117 68-69 1.458461990218467 2117 70-71 -0.6047407901160398 2117 72-73 0.6194235523336111 2117 74-75 1.6358807058220002 2117 76 -0.8450491535669009 2118 1 -0.017205134507385367 2118 2 0.12807410987727863 2118 3 0.2263343335579222 2118 4 1.2425565847330198 2118 5 2.9514024792292766 2118 6 -0.048978719793616676 2118 7 0.09762626188214796 2118 8 2.897667073278356 2118 9 -0.7988753821163641 2118 10-11 2.4906963856247586 2118 12-13 -1.4764817105327417 2118 14-15 1.0260167890286453 2118 16-17 -0.6678002495604467 2118 18-19 -2.9719293734302283 2118 20-21 -0.3386831084533384 2118 22-23 -1.8703426160026169 2118 24-25 0.16070396219920013 2118 26-27 0.6289863230635326 2118 28-29 0.006540981688949898 2118 30-31 -0.9650110061339987 2118 32-33 -1.017732269455589 2118 34-35 -2.280366542402785 2118 36-37 1.9825292336564324 2118 38-39 -0.9314488383261441 2118 40-41 -0.3749638585845787 2118 42-43 -0.1814444238219899 2118 44-45 0.40628409847584734 2118 46-47 0.903279275028158 2118 48-49 -1.0303904619760687 2118 50-51 0.5108145995400726 2118 52-53 -0.050347693743109545 2118 54-55 -0.3429424524954925 2118 56-57 0.7648458935352593 2118 58-59 1.9502411364829904 2118 60-61 0.5420167586153326 2118 62-63 -1.0778311225385977 2118 64-65 -0.16514469831719936 2118 66-67 1.2028407654633995 2118 68-69 -1.8388353070788277 2118 70-71 -0.8614975468727977 2118 72-73 0.22753166044172346 2118 74-75 1.0818266517679476 2118 76 2.857653549135801 2119 1 -0.39471167330424706 2119 2 2.2536190096157256 2119 3 0.9595514216137211 2119 4 -0.03556024177090222 2119 5 1.7003126797523826 2119 6 -1.5014634626009382 2119 7 -2.5545097450925702 2119 8 2.4416949721449654 2119 9 4.726843885538386 2119 10-11 -0.4678912342531838 2119 12-13 1.8822802772615006 2119 14-15 0.5077081578167899 2119 16-17 1.081981790544173 2119 18-19 2.1684367991940086 2119 20-21 1.5828513292101292 2119 22-23 0.21204622445073795 2119 24-25 1.4144092804206458 2119 26-27 0.15296191155524852 2119 28-29 -1.112465121188123 2119 30-31 0.8457998046768118 2119 32-33 2.674944278059666 2119 34-35 -0.03712329915954271 2119 36-37 1.109381892767157 2119 38-39 2.267766506050492 2119 40-41 3.196091067309059 2119 42-43 1.1202120713828911 2119 44-45 2.8827444297748883 2119 46-47 -0.9053519368288612 2119 48-49 0.31878129042149084 2119 50-51 -0.22719760621406948 2119 52-53 1.2639504753937665 2119 54-55 -0.7731952859741327 2119 56-57 -0.2002805232040643 2119 58-59 0.3264399159075779 2119 60-61 1.0180411701236167 2119 62-63 3.3070860527011554 2119 64-65 0.4377323723018094 2119 66-67 -0.7016928003604903 2119 68-69 2.2932483895209934 2119 70-71 1.6938642665535326 2119 72-73 2.269379960354538 2119 74-75 0.7387577764410089 2119 76 0.19679914634591356 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 3.0 12 75.0 13 381.0 14 792.0 15 1117.0 16 1525.0 17 1782.0 18 2054.0 19 1897.0 20 1759.0 21 1268.0 22 866.0 23 533.0 24 418.0 25 283.0 26 197.0 27 127.0 28 93.0 29 69.0 30 59.0 31 45.0 32 47.0 33 34.0 34 18.0 35 13.0 36 6.0 37 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 6.312983551134517 48.02028476692023 8.289448020284768 37.377283661660485 2 12.730091067622554 31.356972163017506 28.670154362849576 27.24278240651037 3 15.093974036039528 32.70683975973648 23.832590583220306 28.366595621003682 4 16.611767745269006 30.452754634114836 21.268488019117743 31.66698960149842 5 7.266033714396435 36.22682942582187 28.934960924885356 27.572175934896336 6 7.666472905767615 32.590583220306144 11.754827875734676 47.98811599819157 7 5.199250791190338 48.22708777368727 11.018536459342505 35.55512497577989 8 4.204611509397404 49.26047923529032 12.794678033972746 33.740231221339535 9 7.388748950461797 35.85868371762578 10.417877672285734 46.33468965962669 10-11 7.521152231479687 36.097655493121486 21.930504424207196 34.45068785119163 12-13 7.249886972808888 31.644384163275852 21.630175030678807 39.475553833236454 14-15 10.540592908351094 34.4054769747465 16.22101659885035 38.83291351805206 16-17 13.637537944842732 32.8134082542143 17.535361364076728 36.01369243686624 18-19 10.750500548989214 30.601304656720274 17.089711296260415 41.5584834980301 20-21 10.075566750629724 31.30530258993735 17.11231673448298 41.506813924949945 22-23 15.649421946651165 31.59594393851321 14.754892462701028 37.9997416521346 24-25 12.190789898598462 35.03842924497837 14.422269585997546 38.348511270425625 26-27 10.882903830007104 35.83284893108571 18.25873538719886 35.02551185170832 28-29 13.392107472712006 31.75095265775367 21.578505457598656 33.27843441193567 30-31 12.468513853904282 31.04372537621908 23.519343796421882 32.96841697345476 32-33 10.543822256668605 35.69721630175031 20.20603242265711 33.55292901892398 34-35 10.337143964347995 33.01039850158239 21.429955434993218 35.22250209907641 36-37 10.456629852095848 31.327908028159918 23.816443841632758 34.39901827811148 38-39 11.131563650455337 26.74223341729639 26.768068203836464 35.358134728411805 40-41 9.064780727249241 28.951107666472907 23.622682942582188 38.36142866369567 42-43 5.386552993605891 26.684105147581217 27.9306335981399 39.99870826067299 44-45 7.02706193890073 28.295549958018473 28.45055867725893 36.22682942582187 46-47 7.689078343990182 31.657301556545892 24.462313505134663 36.19130659432926 48-49 7.863463153135697 33.89523994057999 20.38041723180262 37.86087967448169 50-51 7.815022928373054 32.09649292772718 19.760382354840793 40.32810178905897 52-53 6.610476005942001 31.43124717432022 20.06071174836918 41.8975650713686 54-55 6.729961893689854 31.9996124782019 18.41374410643932 42.856681521668925 56-57 9.907640638119227 31.815539624103856 17.858296195827684 40.418523541949234 58-59 8.654653490925531 31.86720919718401 18.27811147710392 41.200025834786544 60-61 6.27462378092101 29.787508880707875 18.9142930956533 45.02357424271782 62-63 8.102434928631402 30.96622101659885 18.100497319640894 42.83084673512885 64-65 8.45120454692243 33.18155396241039 14.444875024220114 43.92236646644707 66-67 6.710585803784797 37.39262416844281 12.56216495511206 43.33462507266034 68-69 8.893625266421235 37.07291868500936 13.463153135697217 40.57030291287218 70-71 9.097074210424335 36.204223987599306 13.895885810243493 40.802815991732864 72-73 8.128269715171479 34.912484660595496 16.269456823612995 40.689788800620036 74-75 10.750500548989214 33.016857198217394 15.578376283665957 40.65426596912743 76 10.308079829490408 28.540980430149194 18.323322353549052 42.82761738681134 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 3.0 14 4.5 15 5.5 16 5.0 17 4.0 18 7.5 19 11.5 20 14.0 21 16.0 22 23.5 23 33.5 24 41.5 25 47.0 26 50.5 27 57.0 28 83.0 29 106.0 30 116.0 31 134.0 32 176.0 33 210.0 34 219.0 35 279.5 36 351.5 37 372.0 38 414.0 39 517.5 40 628.0 41 732.5 42 788.0 43 783.5 44 846.0 45 942.0 46 971.0 47 985.5 48 962.5 49 940.5 50 956.0 51 926.0 52 839.5 53 746.5 54 710.0 55 653.0 56 566.5 57 509.5 58 482.0 59 427.5 60 316.0 61 248.5 62 238.0 63 206.0 64 166.0 65 122.0 66 83.0 67 80.0 68 67.5 69 48.0 70 34.0 71 27.0 72 24.5 73 18.0 74 15.0 75 16.0 76 13.5 77 11.0 78 11.5 79 12.0 80 11.5 81 7.0 82 3.5 83 4.0 84 5.5 85 5.0 86 1.5 87 0.0 88 3.0 89 5.0 90 2.5 91 0.5 92 0.0 93 0.5 94 1.0 95 2.0 96 3.0 97 3.0 98 3.5 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6587870567719434 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 15483.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.47038687592844 #Duplication Level Percentage of deduplicated Percentage of total 1 99.863645217843 99.33475424659304 2 0.07142393351081099 0.14209132597041918 3 0.01947925459385754 0.058128269715171474 4 0.01947925459385754 0.07750435962022863 5 0.0 0.0 6 0.0 0.0 7 0.006493084864619181 0.04521087644513337 8 0.0 0.0 9 0.006493084864619181 0.058128269715171474 >10 0.012986169729238361 0.28418265194083836 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTGGTC 26 0.16792611251049538 No Hit CTTATACACATCTGACGCTGCCGACGACGTTGCTGGTGTAGATCTCGGTG 18 0.11625653943034295 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.006458696635019053 0.0 16 0.0 0.0 0.0 0.006458696635019053 0.0 17 0.0 0.0 0.0 0.006458696635019053 0.0 18 0.0 0.0 0.0 0.006458696635019053 0.0 19 0.0 0.0 0.0 0.006458696635019053 0.0 20 0.0 0.0 0.0 0.006458696635019053 0.0 21 0.0 0.0 0.0 0.006458696635019053 0.0 22 0.0 0.0 0.0 0.006458696635019053 0.0 23 0.0 0.0 0.0 0.012917393270038106 0.0 24 0.0 0.0 0.0 0.012917393270038106 0.0 25 0.0 0.0 0.0 0.012917393270038106 0.0 26 0.0 0.0 0.0 0.012917393270038106 0.0 27 0.0 0.0 0.0 0.03229348317509526 0.0 28 0.0 0.0 0.0 0.03229348317509526 0.0 29 0.0 0.0 0.0 0.03875217981011432 0.0 30 0.0 0.0 0.0 0.03875217981011432 0.0 31 0.0 0.0 0.0 0.03875217981011432 0.0 32 0.0 0.0 0.0 0.03875217981011432 0.0 33 0.0 0.0 0.0 0.05166957308015242 0.0 34 0.0 0.0 0.0 0.06458696635019053 0.0 35 0.0 0.0 0.0 0.06458696635019053 0.0 36 0.0 0.0 0.0 0.08396305625524769 0.0 37 0.0 0.0 0.0 0.11625653943034296 0.0 38 0.0 0.0 0.0 0.12917393270038105 0.0 39 0.0 0.0 0.0 0.14855002260543823 0.0 40 0.0 0.0 0.0 0.16792611251049538 0.0 41 0.0 0.0 0.0 0.2066782923206097 0.0 42 0.0 0.0 0.0 0.21313698895562874 0.0 43 0.0 0.0 0.0 0.22605438222566687 0.0 44 0.0 0.0 0.0 0.2518891687657431 0.0 45 0.0 0.0 0.0 0.2518891687657431 0.0 46 0.0 0.0 0.0 0.2583478654007621 0.0 47 0.0 0.0 0.0 0.2777239553058193 0.0 48 0.0 0.0 0.0 0.3035587418458955 0.0 49 0.0 0.0 0.0 0.3423109216560098 0.0 50 0.0 0.0 0.0 0.35522831492604795 0.0 51 0.0 0.0 0.0 0.4133565846412194 0.0 52 0.0 0.0 0.0 0.4262739779112575 0.0 53 0.0 0.0 0.0 0.46502615772137185 0.0 54 0.0 0.0 0.0 0.484402247626429 0.0 55 0.0 0.0 0.0 0.5037783375314862 0.0 56 0.0 0.0 0.0 0.5102370341665052 0.0 57 0.0 0.0 0.0 0.5296131240715624 0.0 58 0.0 0.0 0.0 0.5554479106116386 0.0 59 0.0 0.0 0.0 0.5748240005166957 0.0 60 0.0 0.0 0.0 0.6264935735968482 0.0 61 0.0 0.0 0.0 0.6394109668668863 0.0 62 0.0 0.0 0.0 0.6717044500419815 0.0 63 0.0 0.0 0.0 0.6910805399470387 0.0 64 0.0 0.0 0.0 0.7039979332170768 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCCC 15 0.0021858823 69.98058 11 TTACGCC 15 0.0021858823 69.98058 10 TTTACAC 40 9.798896E-9 61.23301 2 CTTTACA 30 7.1243594E-6 59.471947 1 CACAACG 20 0.0068310685 52.485435 68 CCTGACG 20 0.0068310685 52.485435 8 AGGCACA 20 0.0068310685 52.485435 2 TTACACA 50 5.6976205E-8 48.986404 3 ACAGACA 30 5.7079917E-4 46.65372 46 CACCTGA 30 5.7079917E-4 46.65372 6 ACACCTG 35 0.0012193803 39.988903 5 ACCTGAC 35 0.0012193803 39.988903 7 GATCTCG 35 0.0012193803 39.988903 40 AGATCTC 35 0.0012193803 39.988903 39 TAGATCT 35 0.0012193803 39.988903 38 ATCTCGG 40 0.0023497536 34.99029 41 TCTCGGT 40 0.0023497536 34.99029 42 TGTAGAT 50 1.6364703E-4 34.99029 34 GCCGACG 60 1.1430469E-5 34.99029 19 CTGACGC 100 1.1019438E-8 31.49126 9 >>END_MODULE