FastQCFastQC Report
Wed 25 May 2016
SRR1780442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780442_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15483
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT441428.508686946974098No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT306819.815281276238455RNA PCR Primer, Index 27 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC9586.187431376348253No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG5383.47477878964025No Hit
CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC5043.2551831040496024No Hit
TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT2621.692178518374992No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT1701.097978427953239No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG1300.8396305625524769No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1210.7815022928373055No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC1140.7362914163921721No Hit
CTTATAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG950.61357618032681No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGT920.5942000904217529No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC880.5683653038816767No Hit
TTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC850.5489892139766195No Hit
CTTAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC830.5360718207065813No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGT720.4650261577213718No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGTCTT710.45856746108635277No Hit
CTTATCACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC590.38106310146612415No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTCTT550.35522831492604795No Hit
CTTATACACATCTCCGAGCCCACGCGACTTGACCCTATCTCGTATGCCGT550.35522831492604795No Hit
ATACACATCTCCGAGCCCACGAGACTTGAACCTATCTCGTATGCCGTCTT500.32293483175095267TruSeq Adapter, Index 4 (95% over 21bp)
CTTATTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG490.3164761351159336No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG440.28418265194083836No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTCTT440.28418265194083836No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACACTATCTCGTATGCCGT440.28418265194083836No Hit
ACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCT400.2583478654007621RNA PCR Primer, Index 27 (95% over 23bp)
CTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT400.2583478654007621No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGAACCTATCTCGTATGCCGT380.245430472130724No Hit
CTTATACACATCTCCGAGCCCACGAGCCTTGACCCTATCTCGTATGCCGT340.21959568559064782No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTCTCTCGTATGCCGT320.20667829232060972No Hit
CTTATACACATCTCCGAGCACACGAGACTTGACCCTATCTCGTATGCCGT300.1937608990505716No Hit
ATACACATCTCCGAGCCCACGAGACTTGACACTATCTCGTATGCCGTCTT270.17438480914551444Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTAGTATGCCGT270.17438480914551444No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCG260.16792611251049538No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCG250.16146741587547633No Hit
ATACACATCTCCGAGCCCACGAGAATTGACCCTATCTCGTATGCCGTCTT230.14855002260543823No Hit
TCTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT220.14209132597041918No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTCTCTCGTATGCCGTCTT220.14209132597041918No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTC220.14209132597041918No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCAGT210.13563262933540013No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTCTGCCGTCTT210.13563262933540013No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTC200.12917393270038105No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCCCCTATCTCGTATGCCGT190.122715236065362No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCCT190.122715236065362No Hit
CTTATACACATCTCCGAGCCCAAGAGACTTGACCCTATCTCGTATGCCGT180.11625653943034295No Hit
ATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTGCTT180.11625653943034295Illumina PCR Primer Index 4 (96% over 26bp)
CTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT180.11625653943034295RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATATCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT180.11625653943034295No Hit
CTTATACACATCTACGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT180.11625653943034295No Hit
TCTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT180.11625653943034295No Hit
ATACACATCTCCGAGCCCACGAGACTTGCCCCTATCTCGTATGCCGTCTT170.10979784279532391RNA PCR Primer, Index 27 (95% over 21bp)
CTTATATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC170.10979784279532391No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTCTGCCGT160.10333914616030486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACA950.070.000011
TTTACAC950.070.000012
CGCGACT207.684063E-570.023
GCGACTT207.684063E-570.024
CACGCGA207.684063E-570.021
CCACGCG207.684063E-570.020
TTATTAC150.00218347870.02
CCCACGC207.684063E-570.019
ATTACAC150.00218347870.04
CTTATTA150.00218347870.01
TATTACA150.00218347870.03
TCTTTAT309.590076E-870.01
ACGCGAC207.684063E-570.022
CGACTTG207.684063E-570.025
TCTTAAT150.00218347870.01
AACATAT150.00218347870.067
CTTTATA352.7870556E-760.0000042
TTTATAC352.7870556E-760.0000043
CTATACA1000.059.5000041
CTTATAC6950.059.424461
Produced by FastQC (version 0.11.5)