##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780442_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15483 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.89181683136343 34.0 34.0 34.0 32.0 34.0 2 33.204030226700255 34.0 34.0 34.0 32.0 34.0 3 33.353161532002844 34.0 34.0 34.0 33.0 34.0 4 33.458761221985405 34.0 34.0 34.0 34.0 34.0 5 33.50494090292579 34.0 34.0 34.0 34.0 34.0 6 37.34909255312278 38.0 37.0 38.0 37.0 38.0 7 36.912807595427246 38.0 37.0 38.0 36.0 38.0 8 36.938061099270165 38.0 37.0 38.0 35.0 38.0 9 36.65013240328102 38.0 37.0 38.0 34.0 38.0 10-11 36.40789898598463 38.0 37.0 38.0 34.0 38.0 12-13 36.038203190596136 38.0 36.5 38.0 33.0 38.0 14-15 36.06100238971776 38.0 36.0 38.0 33.0 38.0 16-17 35.955531873667894 38.0 36.0 38.0 32.0 38.0 18-19 36.0225731447394 38.0 36.5 38.0 32.5 38.0 20-21 35.88209649292773 38.0 36.0 38.0 31.0 38.0 22-23 35.86830717561196 38.0 36.5 38.0 32.5 38.0 24-25 34.606116385713364 37.0 34.0 38.0 28.0 38.0 26-27 34.86000775043596 37.0 34.0 38.0 29.0 38.0 28-29 34.8893948201253 37.0 35.0 38.0 27.0 38.0 30-31 34.2440418523542 37.0 34.0 38.0 25.0 38.0 32-33 34.69285668152167 37.0 35.0 38.0 27.0 38.0 34-35 33.900277723955305 37.0 34.0 38.0 24.5 38.0 36-37 33.623393399212034 37.0 34.0 38.0 23.0 38.0 38-39 31.832396822321257 36.0 31.0 37.0 11.0 38.0 40-41 33.42601563004585 37.0 34.0 37.5 23.5 38.0 42-43 34.70919718400827 37.0 34.5 38.0 28.5 38.0 44-45 35.06597558612672 37.0 35.0 38.0 29.0 38.0 46-47 35.05987211780662 37.0 35.0 38.0 29.0 38.0 48-49 34.576018859394175 37.0 35.0 38.0 27.5 38.0 50-51 34.38532584124523 37.0 34.0 38.0 25.5 38.0 52-53 34.06329522702319 37.0 34.0 38.0 24.0 38.0 54-55 34.53516760317768 37.0 34.5 38.0 26.0 38.0 56-57 34.31156752567332 37.0 34.0 38.0 25.0 38.0 58-59 34.00519925079119 37.0 34.0 38.0 24.0 38.0 60-61 34.004714848543564 37.0 34.0 38.0 24.5 38.0 62-63 34.9079312794678 37.0 35.5 38.0 27.0 38.0 64-65 34.81993153781566 37.0 35.0 38.0 26.5 38.0 66-67 32.54030226700252 36.5 31.0 38.0 21.0 38.0 68-69 28.08486727378415 32.5 21.5 37.0 11.0 38.0 70-71 23.905154039914745 25.5 15.0 33.5 10.5 37.0 72-73 19.90612284441 21.0 11.0 25.0 10.5 34.0 74-75 19.343344313117612 19.0 11.0 25.0 11.0 34.0 76 19.682232125557064 20.0 11.0 25.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5619644538185682 1101 2 0.6516148223045448 1101 3 0.35722061590170284 1101 4 0.4166767297439833 1101 5 0.47676909157791414 1101 6 -1.4736997925970883 1101 7 -0.2117696613976321 1101 8 0.33603400039588394 1101 9 0.42612421812953016 1101 10-11 0.32539326496113574 1101 12-13 0.8155478542419132 1101 14-15 0.1885635889937305 1101 16-17 -0.654165805176774 1101 18-19 0.6874172600681945 1101 20-21 0.011666611529307147 1101 22-23 0.35315631368123235 1101 24-25 -1.7758484262444156 1101 26-27 0.5982133353361618 1101 28-29 0.7012363669268353 1101 30-31 1.2340714971629367 1101 32-33 -1.5323604196785539 1101 34-35 -0.5446665685345735 1101 36-37 -0.5754981504994703 1101 38-39 -0.9563700363986563 1101 40-41 1.4448258466601658 1101 42-43 1.3689700453199336 1101 44-45 -0.584172437884888 1101 46-47 -0.20732462338773416 1101 48-49 -2.813509752288727 1101 50-51 -2.0887627897916303 1101 52-53 -2.487245347815989 1101 54-55 -1.70083612912169 1101 56-57 -2.5815873507183014 1101 58-59 -2.3865559530263596 1101 60-61 -4.221280459339553 1101 62-63 -2.9784427537665756 1101 64-65 -2.812989367954323 1101 66-67 -4.07125729495376 1101 68-69 -3.0236409725489537 1101 70-71 -1.788484326940953 1101 72-73 -1.9408825074559637 1101 74-75 -1.1911294707192432 1101 76 -1.267762592709694 1102 1 -1.6645061344167225 1102 2 -0.2601498835778102 1102 3 0.42633826296052746 1102 4 0.49167672974398613 1102 5 0.522357326872033 1102 6 0.5542413838734959 1102 7 0.6176421033082491 1102 8 0.7051516474547128 1102 9 0.8643595122471766 1102 10-11 1.2187756179023097 1102 12-13 0.947165501300745 1102 14-15 0.2731224125231364 1102 16-17 0.514951841882052 1102 18-19 -0.5978768575788607 1102 20-21 0.7168136703528418 1102 22-23 1.3980092548577048 1102 24-25 -6.0162896027150055 1102 26-27 -0.25619842936972503 1102 28-29 0.9843246022209513 1102 30-31 2.557600908927647 1102 32-33 0.8617572273802736 1102 34-35 1.859009902053657 1102 36-37 -2.8335863857935877 1102 38-39 1.7223064341895764 1102 40-41 2.1536493760719324 1102 42-43 1.8130876923787582 1102 44-45 2.004062856232764 1102 46-47 2.2309106707299193 1102 48-49 -4.109097987582842 1102 50-51 -0.20199808390928098 1102 52-53 0.5406958286545986 1102 54-55 0.7043109297018333 1102 56-57 2.163265590458167 1102 58-59 0.8877087528559855 1102 60-61 -6.341868694633668 1102 62-63 -1.2049133420018663 1102 64-65 0.39509886733979016 1102 66-67 2.601536822693298 1102 68-69 -0.3508468549018957 1102 70-71 -0.8068666798821269 1102 72-73 -1.8055883898089071 1102 74-75 -0.37495300013100774 1102 76 2.9175315249373632 1103 1 0.6641383668620477 1103 2 0.6516148223045448 1103 3 -0.08517068844612652 1103 4 0.31776368626572804 1103 5 0.24742126549096355 1103 6 0.48390890305508094 1103 7 0.9795346864284511 1103 8 0.5197296525698007 1103 9 0.23264595725996884 1103 10-11 -0.3675415176475596 1103 12-13 -0.705647797931988 1103 14-15 0.16954184986329324 1103 16-17 0.4490950643884446 1103 18-19 1.1374172600681973 1103 20-21 0.2187318289206175 1103 22-23 0.7113084875942803 1103 24-25 -3.782913643635723 1103 26-27 -0.8572214472725364 1103 28-29 -0.3993071113340392 1103 30-31 1.7123323667281554 1103 32-33 1.9915526237997128 1103 34-35 0.9504421271175971 1103 36-37 0.35439315384835623 1103 38-39 1.3137386592535165 1103 40-41 1.0296084553558202 1103 42-43 -0.5353777807670212 1103 44-45 1.307131909941198 1103 46-47 1.6426753766122673 1103 48-49 -2.3124227957669845 1103 50-51 0.4623241667301059 1103 52-53 1.4160155217492303 1103 54-55 0.46709865348699964 1103 56-57 0.5200430840643051 1103 58-59 -1.1653603008524485 1103 60-61 -4.893019589774333 1103 62-63 -1.7023557972448344 1103 64-65 -0.4124458896934513 1103 66-67 -1.1772355558233265 1103 68-69 1.316032940494523 1103 70-71 0.9207548034938284 1103 72-73 0.28520444906577325 1103 74-75 1.5208270510198894 1103 76 0.5735417551163913 1104 1 -0.4982396278140797 1104 2 0.6107984957739347 1104 3 0.5914042893710914 1104 4 -0.1817926580111191 1104 5 -0.9380268267894323 1104 6 0.2523206155661768 1104 7 0.6596589100309345 1104 8 0.5640952248856834 1104 9 1.1332670752723857 1104 10-11 -0.09935163299805083 1104 12-13 0.5765172419970241 1104 14-15 1.0903493032794458 1104 16-17 0.5014464397211853 1104 18-19 1.3670090968028958 1104 20-21 -0.18654767418497187 1104 22-23 0.2605542728649084 1104 24-25 0.8876719819188494 1104 26-27 0.7681112945198336 1104 28-29 1.5953690199880555 1104 30-31 1.8361123134894726 1104 32-33 0.6747824374643088 1104 34-35 0.18313955391440118 1104 36-37 2.4686345025617484 1104 38-39 2.9494973105401243 1104 40-41 1.8448258466601644 1104 42-43 0.7842761677689083 1104 44-45 1.4566438886457291 1104 46-47 1.8314508868163486 1104 48-49 0.6063882068949482 1104 50-51 0.9729719040859166 1104 52-53 1.4377546521840117 1104 54-55 1.0356434627150364 1104 56-57 0.3115248941796551 1104 58-59 -1.253647789761061 1104 60-61 0.07259709168085493 1104 62-63 -0.1457896925420883 1104 64-65 1.0469596116375115 1104 66-67 -0.10314505005580088 1104 68-69 2.2199814764306396 1104 70-71 0.5520768975488437 1104 72-73 0.34687259458485187 1104 74-75 -0.07123151153556861 1104 76 -0.08306871515867442 1105 1 0.7230755649296796 1105 2 0.4071703778600977 1105 3 0.4544428381239243 1105 4 -0.752767714700461 1105 5 -0.09267535286652873 1105 6 0.07907798518068887 1105 7 0.7993414497134737 1105 8 -1.9334104440485547 1105 9 0.8983464403517516 1105 10-11 -0.04266229059442139 1105 12-13 -1.6969521457580754 1105 14-15 -0.22393641100626382 1105 16-17 0.3152786392676745 1105 18-19 0.020750593401530182 1105 20-21 -0.02305561069290718 1105 22-23 -0.2176770196521005 1105 24-25 0.16998490708891012 1105 26-27 -0.5212311091082853 1105 28-29 0.49151414470460963 1105 30-31 1.5562937193851596 1105 32-33 1.5954173580992261 1105 34-35 -2.1710554574234635 1105 36-37 0.153668516167194 1105 38-39 0.6422410747124587 1105 40-41 -0.17739637556206134 1105 42-43 -0.26991884356895923 1105 44-45 0.27138311767066625 1105 46-47 0.9926753766122616 1105 48-49 -1.1273986411776136 1105 50-51 1.3584594324305996 1105 52-53 -0.20668979226043405 1105 54-55 1.5755527597671986 1105 56-57 1.3586904270594715 1105 58-59 -1.2185003974708053 1105 60-61 0.6009417628826768 1105 62-63 -1.9339983093221278 1105 64-65 1.0356217431567885 1105 66-67 -0.5670906282870973 1105 68-69 -0.5972520836600665 1105 70-71 0.5545712286146056 1105 72-73 -1.0742158407892965 1105 74-75 1.417203862614091 1105 76 -0.8399848149319169 1106 1 0.15482159667571693 1106 2 -0.7293375586478348 1106 3 0.4417444254255116 1106 4 0.18215292022017593 1106 5 0.4089119487207711 1106 6 -0.03679503069233192 1106 7 -0.29272204235001453 1106 8 -0.11277552341363872 1106 9 -0.7476853056799939 1106 10-11 0.48908374115161735 1106 12-13 1.1411430923371597 1106 14-15 -0.0763173633872185 1106 16-17 -0.529165805176774 1106 18-19 0.11360773625867893 1106 20-21 0.07535708771979444 1106 22-23 0.37279917082409497 1106 24-25 -0.22287223576822868 1106 26-27 0.7851180972409182 1106 28-29 0.7161173193077843 1106 30-31 1.1388334019248418 1106 32-33 -0.07521756253569123 1106 34-35 1.3345000981320965 1106 36-37 0.8512875637862436 1106 38-39 -0.18315575068437084 1106 40-41 1.0710163228506389 1106 42-43 0.8880176643675526 1106 44-45 1.6539228002103528 1106 46-47 1.9402944242313183 1106 48-49 1.1472045334255583 1106 50-51 0.3862372102083711 1106 52-53 0.40204036646972696 1106 54-55 0.1342829184973553 1106 56-57 -0.8857540173849685 1106 58-59 1.142610713640309 1106 60-61 1.1049100168509227 1106 62-63 1.4477477224238982 1106 64-65 -1.5040607965257564 1106 66-67 1.3900522288557582 1106 68-69 2.3781447417367616 1106 70-71 -0.25574623170285804 1106 72-73 1.3400698734964145 1106 74-75 -1.2208913754811483 1106 76 1.8965231215760205 1107 1 0.8551017087205324 1107 2 -0.15230674632290686 1107 3 0.27927943943111444 1107 4 0.0799120238616311 1107 5 -0.15411326136325698 1107 6 0.4463982466185925 1107 7 0.794112691543539 1107 8 0.24436733372922248 1107 9 -1.3317189191253718 1107 10-11 0.6011285590787807 1107 12-13 0.6089302071830929 1107 14-15 0.3368479027192208 1107 16-17 0.6571087046271487 1107 18-19 0.5932996130093713 1107 20-21 1.1972058272155905 1107 22-23 0.41761709799495605 1107 24-25 0.2876319659124462 1107 26-27 0.20948784514008167 1107 28-29 1.3274618571229126 1107 30-31 0.36152247755509137 1107 32-33 1.4450905607136022 1107 34-35 -0.611578333240459 1107 36-37 2.1517077318534703 1107 38-39 1.4330907479150667 1107 40-41 0.7761983956797778 1107 42-43 1.4013229864964032 1107 44-45 1.298180503291583 1107 46-47 1.6279694942593252 1107 48-49 1.6850196594759836 1107 50-51 2.4499627004044484 1107 52-53 1.4377546521840117 1107 54-55 1.449408968917517 1107 56-57 2.4573832375169857 1107 58-59 1.9710420861893212 1107 60-61 2.270876403405545 1107 62-63 0.47645920701774 1107 64-65 1.4539223967515582 1107 66-67 -1.6827769027968955 1107 68-69 -0.664572345097973 1107 70-71 -0.8852980524311498 1107 72-73 -0.942843291769691 1107 74-75 -0.012207902091791567 1107 76 -0.8569782789842044 1108 1 -1.6547022128480968 1108 2 -0.2769566062668858 1108 3 0.03698252066360652 1108 4 0.32501006307732183 1108 5 0.09938813919696798 1108 6 0.010824016926719082 1108 7 -0.12605537568335023 1108 8 -1.0175374281755438 1108 9 0.395171837177152 1108 10-11 0.41765516972304084 1108 12-13 1.105428806622868 1108 14-15 0.852254065184205 1108 16-17 -1.2077372337482046 1108 18-19 0.8278934505443871 1108 20-21 1.4324999448626414 1108 22-23 -0.34148654346162743 1108 24-25 1.3604610975651 1108 26-27 0.4041657162885386 1108 28-29 -1.1172160140255443 1108 30-31 1.1745476876391265 1108 32-33 0.7938300565119221 1108 34-35 -0.11788085424886674 1108 36-37 1.6012875637862436 1108 38-39 2.0192252016965853 1108 40-41 1.023397275231595 1108 42-43 0.3284938548437424 1108 44-45 0.8563037525913018 1108 46-47 1.3450563289932163 1108 48-49 1.2900616762827042 1108 50-51 0.8505229244940793 1108 52-53 1.104421318850683 1108 54-55 -1.4609551767407467 1108 56-57 0.3523412207102652 1108 58-59 0.44022976125935287 1108 60-61 1.6287195406604482 1108 62-63 1.8525096271858033 1108 64-65 1.1745106320456742 1108 66-67 2.0924331812367214 1108 68-69 3.294811408403426 1108 70-71 -1.7438414697980953 1108 72-73 0.1972127306392757 1108 74-75 0.743394338804567 1108 76 1.539380264433163 1109 1 -1.988035546181429 1109 2 0.1733539527393262 1109 3 0.5452640941625688 1109 4 0.4264593384396349 1109 5 0.22568213505617507 1109 6 -0.5160910969449191 1109 7 -0.06394357444111165 1109 8 0.5632079134393635 1109 9 0.3630807398686642 1109 10-11 0.5889802214828705 1109 12-13 1.0878304629375748 1109 14-15 1.0825853281241677 1109 16-17 1.3730081078667098 1109 18-19 0.898286825285588 1109 20-21 1.1209057419640942 1109 22-23 0.43956935715949896 1109 24-25 0.20621679114688618 1109 26-27 0.13190898751007296 1109 28-29 -0.8558288504644693 1109 30-31 0.9949410623803274 1109 32-33 0.1328569716257917 1109 34-35 -1.647383959838919 1109 36-37 1.2022192408048724 1109 38-39 2.2050430070796097 1109 40-41 2.0622171510079923 1109 42-43 1.7689700453199322 1109 44-45 0.9701753882020654 1109 46-47 0.7187623331340021 1109 48-49 0.6549685085808363 1109 50-51 -0.026806268052503412 1109 52-53 -0.5839844782507697 1109 54-55 -0.261162216078219 1109 56-57 0.030912649281695792 1109 58-59 -0.8610124747654879 1109 60-61 1.639589105877839 1109 62-63 0.8411224636247283 1109 64-65 1.217988892915237 1109 66-67 0.822764444176677 1109 68-69 0.424728592668437 1109 70-71 1.0620591513199145 1109 72-73 0.29607401428316393 1109 74-75 -0.6530859924583758 1109 76 -1.8177625927096948 1110 1 -0.21720221284809327 1110 2 -0.24421851102879089 1110 3 0.2780539492350371 1110 4 -0.04998993692267817 1110 5 0.551769091577917 1110 6 0.09713354073624458 1110 7 0.35906367193570077 1110 8 0.3902006670625511 1110 9 0.7969575514628673 1110 10-11 -1.1641900683721929 1110 12-13 0.9551311875752475 1110 14-15 1.459396922327059 1110 16-17 0.5854175281565617 1110 18-19 -0.2688327399318027 1110 20-21 1.3699999448626414 1110 22-23 -0.26559368631876623 1110 24-25 -2.246681759577754 1110 26-27 0.898213335336159 1110 28-29 0.7637363669268353 1110 30-31 -0.14092850283706326 1110 32-33 0.9113895803214476 1110 34-35 0.35533343146542506 1110 36-37 1.6370018495005283 1110 38-39 1.176963296934673 1110 40-41 -0.6239241533398356 1110 42-43 0.4148033786532679 1110 44-45 1.3949942287817834 1110 46-47 0.6426753766122673 1110 48-49 2.351073581044602 1110 50-51 -0.6866794564583003 1110 52-53 1.1565046521840117 1110 54-55 -0.08000279578836 1110 56-57 1.0413293159483672 1110 58-59 0.27802738030697327 1110 60-61 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58-59 -3.9129448419152517 2116 60-61 -1.4407249037839946 2116 62-63 0.20766835734453792 2116 64-65 -1.686600479065433 2116 66-67 0.21346492726846122 2116 68-69 -2.4889187503267323 2116 70-71 -0.7120954380520637 2116 72-73 0.8757841592106992 2116 74-75 -1.927240581830354 2116 76 -0.7899848149319162 2117 1 1.011964453818571 2117 2 0.5705337412234641 2117 3 0.4430314267125155 2117 4 0.49167672974398613 2117 5 0.551769091577917 2117 6 0.6202191263218282 2117 7 0.7909330413050668 2117 8 0.7218448112067009 2117 9 -0.5346865926812825 2117 10-11 -0.8519715999037274 2117 12-13 -2.0564116052175407 2117 14-15 -0.09690938397923787 2117 16-17 -1.6778144538254196 2117 18-19 -0.6666367939858588 2117 20-21 -0.21164419928149414 2117 22-23 -0.5248842268593066 2117 24-25 -1.8525376154336115 2117 26-27 -0.6930028808800586 2117 28-29 -1.8646420114515436 2117 30-31 -1.9956582325667895 2117 32-33 -3.776955014273149 2117 34-35 0.5394550530870461 2117 36-37 -2.2346197721210928 2117 38-39 0.8959948284662147 2117 40-41 -0.18220118036686728 2117 42-43 0.018970045319932183 2117 44-45 0.5644762107637575 2117 46-47 -2.0667840828471924 2117 48-49 -0.4493205630995405 2117 50-51 -0.4583573843862254 2117 52-53 1.5593762738056327 2117 54-55 -0.7917145075000747 2117 56-57 0.41604778441682555 2117 58-59 -1.2064883854587976 2117 60-61 0.203043864984771 2117 62-63 -0.670334645658464 2117 64-65 -2.818732611197568 2117 66-67 -2.3770005381970023 2117 68-69 -1.2212761076840906 2117 70-71 0.39901567305904706 2117 72-73 -1.986828453401909 2117 74-75 0.2244110698212971 2117 76 -0.3042490791961825 2118 1 0.2281806700347886 2118 2 0.5435067141964396 2118 3 -0.7731847895037021 2118 4 -0.07589083782357875 2118 5 0.4166339564427801 2118 6 0.26886777497047376 2118 7 0.8720141223861475 2118 8 0.7488718382337254 2118 9 -1.8049568629515491 2118 10-11 -1.0411607890929133 2118 12-13 -0.7996548484607828 2118 14-15 0.7679554808856182 2118 16-17 -2.1913279673389354 2118 18-19 -0.49096111831018163 2118 20-21 -0.46840095603825205 2118 22-23 0.32646712449204074 2118 24-25 1.0663813034853078 2118 26-27 -3.071381259258434 2118 28-29 -0.7565339033434384 2118 30-31 -0.6443068812154422 2118 32-33 1.0879098505917213 2118 34-35 1.1881037017356988 2118 36-37 -2.9643495018508226 2118 38-39 -0.21211327964189763 2118 40-41 1.236717738552052 2118 42-43 0.16761869396857776 2118 44-45 -1.5166048703173232 2118 46-47 0.6899726739095584 2118 48-49 -0.0439151576941299 2118 50-51 -1.728627654656492 2118 52-53 -3.251434537005178 2118 54-55 -1.6971199129054781 2118 56-57 0.956588324957373 2118 58-59 -1.8416235205939273 2118 60-61 -1.7564155944746886 2118 62-63 -2.1297941051179308 2118 64-65 0.03261874015378652 2118 66-67 0.25813459693812746 2118 68-69 -2.234789621197603 2118 70-71 -0.9928762188328442 2118 72-73 -0.2030446696181265 2118 74-75 3.7784651238753533 2118 76 0.5335887586416561 2119 1 0.753899937689539 2119 2 -1.9935464680180353 2119 3 0.019317390095253018 2119 4 -0.6373555283205334 2119 5 -0.2224244568091791 2119 6 -0.04063527646805909 2119 7 -1.460156758171827 2119 8 0.23361464555717504 2119 9 -0.4980693302575645 2119 10-11 -0.0032357672969283158 2119 12-13 0.177241402629015 2119 14-15 -1.236839636812718 2119 16-17 -1.1259399987251584 2119 18-19 -1.6609698367059949 2119 20-21 -2.400833388470687 2119 22-23 -0.41982755728651 2119 24-25 1.0947160898846136 2119 26-27 1.0716004321103512 2119 28-29 1.2234137862816752 2119 30-31 1.9437489165177766 2119 32-33 0.28538151580531945 2119 34-35 -0.30797302014747885 2119 36-37 -2.79041750533818 2119 38-39 -4.388224875108335 2119 40-41 -2.1067870565656435 2119 42-43 -0.15844930951877956 2119 44-45 0.673892078244144 2119 46-47 -0.13958268790386086 2119 48-49 -0.42883233293389367 2119 50-51 1.5555920489180508 2119 52-53 0.16356110379691557 2119 54-55 -0.5584974194442722 2119 56-57 -2.6061841249118487 2119 58-59 1.3759536073365553E-4 2119 60-61 -1.411603039984712 2119 62-63 -2.133281463444 2119 64-65 -1.0109732389220696 2119 66-67 -1.2627895530182798 2119 68-69 2.2525687048703986 2119 70-71 2.2538543827364634 2119 72-73 -0.6537857332624171 2119 74-75 -0.4129036642676276 2119 76 -2.8177625927096948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 3.0 22 6.0 23 19.0 24 44.0 25 75.0 26 155.0 27 272.0 28 383.0 29 656.0 30 909.0 31 1310.0 32 1840.0 33 2588.0 34 3334.0 35 3095.0 36 742.0 37 51.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.6329522702318672 28.44409998062391 11.302719111283343 59.62022863786088 2 0.361687011561067 3.158302654524317 87.457211134793 9.022799199121618 3 0.44565006781631467 33.559387715559005 63.46315313569721 2.5318090809274687 4 0.3164761351159336 47.00639410966867 23.567784021184526 29.10934573403087 5 0.38106310146612415 50.4747142026739 45.456306917264094 3.6879157785958796 6 0.47148485435639087 48.349803009752634 8.344636052444617 42.83407608344636 7 0.8137957760124006 51.04307950655558 4.127107149777174 44.01601756765485 8 0.5037783375314862 47.426209390944905 28.463476070528966 23.60653620099464 9 0.6587870567719434 23.567784021184526 3.229348317509526 72.544080604534 10-11 0.6878511916295291 26.777756248788993 43.082735903894594 29.451656655686882 12-13 14.354453271329845 3.029128721823936 33.94045081702512 48.675967189821094 14-15 22.9832719757153 15.923916553639476 6.490990118194148 54.601821352451076 16-17 33.42052573790609 28.4731641154815 0.68139249499451 37.4249176516179 18-19 26.67118775431118 20.5418846476781 0.6490990118194149 52.137828586191304 20-21 16.233933992120388 10.127236323709875 0.8137957760124006 72.82503390815734 22-23 22.99941871730285 40.32487244074146 0.539301169024091 36.136407672931604 24-25 33.288122456888196 44.639281792934185 1.240069753923658 20.832525996253956 26-27 26.974746496157074 33.93722146870761 29.13195117225344 9.95608086288187 28-29 17.341600465026158 20.357811793580055 36.753213201575925 25.547374539817863 30-31 27.885422721694763 9.988374346056967 31.09216560098172 31.03403733126655 32-33 6.652457534069625 25.495704966737716 16.750629722921914 51.101207776270755 34-35 0.8880707873151199 18.465413679519475 22.789511076664727 57.85700445650068 36-37 0.9365110120777627 28.744429374152297 46.315313569721624 24.00374604404831 38-39 14.273719563392106 8.619130659432926 34.17619324420332 42.93095653297165 40-41 9.513660143383065 15.203771878834852 41.43899761028224 33.84357036749984 42-43 38.14829167474004 9.507201446748047 45.06232642252793 7.282180455983983 44-45 11.309177807918362 24.769101595298068 34.42162371633405 29.50009688044953 46-47 39.662856035652005 2.6545243169928305 20.606471614028287 37.076148033326874 48-49 29.700316476135114 1.1205838661758056 23.306206807466253 45.872892850222826 50-51 6.6589162307046434 1.2239230123361107 48.58554543693083 43.531615320028415 52-53 14.099334754246593 1.411225214751663 68.62365174707745 15.865788283924303 54-55 9.290835109474909 1.343408900083963 49.34767163986308 40.01808435057805 56-57 38.09662210165988 1.6760317767874442 30.058774139378674 30.168571982173997 58-59 10.986242976167409 29.85532519537557 51.818123102757866 7.340308725699154 60-61 24.520441774849836 64.97448814829167 8.65788283924304 1.8471872376154492 62-63 2.2670025188916876 95.37880255764387 0.7459794613447006 1.6082154621197442 64-65 0.3778337531486146 97.17109087386166 0.6975392365820577 1.753536136407673 66-67 0.36814570819608605 94.68126332106182 0.8622360007750437 4.08835496996706 68-69 0.9978686301104437 85.1546857844087 2.9451656655686884 10.90227991991216 70-71 2.170122069366402 75.44726474197508 5.567396499386424 16.815216689272106 72-73 3.226118969192017 61.74191048246463 9.113220952011885 25.91874959633146 74-75 3.997933217076794 59.08738616547181 9.42000904217529 27.494671575276108 76 2.8805786992184976 61.609507201446746 8.51902086159013 26.990893237744622 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.0 21 1.0 22 0.5 23 0.0 24 2.5 25 5.0 26 2.5 27 0.0 28 0.5 29 1.0 30 2.0 31 5.0 32 6.5 33 6.0 34 6.5 35 10.5 36 19.0 37 24.0 38 57.5 39 315.5 40 1424.5 41 3088.0 42 3867.0 43 3760.5 44 3026.0 45 1858.5 46 1319.0 47 987.0 48 472.0 49 205.5 50 122.0 51 93.0 52 45.5 53 28.0 54 29.0 55 22.5 56 13.0 57 8.0 58 6.0 59 5.5 60 3.5 61 1.5 62 1.0 63 2.0 64 2.5 65 1.5 66 1.0 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 15483.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.268358845185043 #Duplication Level Percentage of deduplicated Percentage of total 1 79.73773265651438 12.174643157010916 2 8.41793570219966 2.570561260737583 3 3.5956006768189512 1.6469676419298585 4 1.3959390862944163 0.8525479558225151 5 1.015228426395939 0.7750435962022864 6 0.8037225042301184 0.7362914163921721 7 0.676818950930626 0.723374023122134 8 0.3807106598984772 0.4650261577213718 9 0.338409475465313 0.4650261577213718 >10 2.7918781725888326 8.32525996253956 >50 0.4230118443316413 4.876315959439386 >100 0.21150592216582065 5.147581218110185 >500 0.12690355329949238 12.917393270038104 >1k 0.08460236886632826 48.32396822321255 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT 4414 28.508686946974098 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT 3068 19.815281276238455 RNA PCR Primer, Index 27 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 958 6.187431376348253 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 538 3.47477878964025 No Hit CTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 504 3.2551831040496024 No Hit TATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT 262 1.692178518374992 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGT 170 1.097978427953239 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 130 0.8396305625524769 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 121 0.7815022928373055 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 114 0.7362914163921721 No Hit CTTATAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 95 0.61357618032681 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGT 92 0.5942000904217529 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 88 0.5683653038816767 No Hit TTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 85 0.5489892139766195 No Hit CTTAACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 83 0.5360718207065813 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGT 72 0.4650261577213718 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCGTCTT 71 0.45856746108635277 No Hit CTTATCACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTC 59 0.38106310146612415 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTCTT 55 0.35522831492604795 No Hit CTTATACACATCTCCGAGCCCACGCGACTTGACCCTATCTCGTATGCCGT 55 0.35522831492604795 No Hit ATACACATCTCCGAGCCCACGAGACTTGAACCTATCTCGTATGCCGTCTT 50 0.32293483175095267 TruSeq Adapter, Index 4 (95% over 21bp) CTTATTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 49 0.3164761351159336 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG 44 0.28418265194083836 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTCTT 44 0.28418265194083836 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACACTATCTCGTATGCCGT 44 0.28418265194083836 No Hit ACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCT 40 0.2583478654007621 RNA PCR Primer, Index 27 (95% over 23bp) CTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCT 40 0.2583478654007621 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGAACCTATCTCGTATGCCGT 38 0.245430472130724 No Hit CTTATACACATCTCCGAGCCCACGAGCCTTGACCCTATCTCGTATGCCGT 34 0.21959568559064782 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTCTCTCGTATGCCGT 32 0.20667829232060972 No Hit CTTATACACATCTCCGAGCACACGAGACTTGACCCTATCTCGTATGCCGT 30 0.1937608990505716 No Hit ATACACATCTCCGAGCCCACGAGACTTGACACTATCTCGTATGCCGTCTT 27 0.17438480914551444 Illumina PCR Primer Index 6 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTAGTATGCCGT 27 0.17438480914551444 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCG 26 0.16792611251049538 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATATCGTATGCCG 25 0.16146741587547633 No Hit ATACACATCTCCGAGCCCACGAGAATTGACCCTATCTCGTATGCCGTCTT 23 0.14855002260543823 No Hit TCTTTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT 22 0.14209132597041918 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTCTCTCGTATGCCGTCTT 22 0.14209132597041918 No Hit CTTTACACATCTCCGAGCCCACGAGACTTGACCCTAACTCGTATGCCGTC 22 0.14209132597041918 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCAGT 21 0.13563262933540013 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTCTGCCGTCTT 21 0.13563262933540013 No Hit CTTTACACATCTCCGAGCCCACGAGACTTGACCCTACCTCGTATGCCGTC 20 0.12917393270038105 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCCCCTATCTCGTATGCCGT 19 0.122715236065362 No Hit ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCCT 19 0.122715236065362 No Hit CTTATACACATCTCCGAGCCCAAGAGACTTGACCCTATCTCGTATGCCGT 18 0.11625653943034295 No Hit ATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTGCTT 18 0.11625653943034295 Illumina PCR Primer Index 4 (96% over 26bp) CTACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT 18 0.11625653943034295 RNA PCR Primer, Index 27 (95% over 21bp) CTTATACACATATCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT 18 0.11625653943034295 No Hit CTTATACACATCTACGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT 18 0.11625653943034295 No Hit TCTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT 18 0.11625653943034295 No Hit ATACACATCTCCGAGCCCACGAGACTTGCCCCTATCTCGTATGCCGTCTT 17 0.10979784279532391 RNA PCR Primer, Index 27 (95% over 21bp) CTTATATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC 17 0.10979784279532391 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTCTGCCGT 16 0.10333914616030486 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.006458696635019053 0.0 16 0.0 0.0 0.0 0.006458696635019053 0.0 17 0.0 0.0 0.0 0.006458696635019053 0.0 18 0.0 0.0 0.0 0.006458696635019053 0.0 19 0.0 0.0 0.0 0.006458696635019053 0.0 20 0.0 0.0 0.0 0.006458696635019053 0.0 21 0.0 0.0 0.0 0.006458696635019053 0.0 22 0.0 0.0 0.0 0.006458696635019053 0.0 23 0.0 0.0 0.0 0.012917393270038106 0.0 24 0.0 0.0 0.0 0.01937608990505716 0.0 25 0.0 0.0 0.0 0.01937608990505716 0.0 26 0.0 0.0 0.0 0.01937608990505716 0.0 27 0.0 0.0 0.0 0.03229348317509526 0.0 28 0.0 0.0 0.0 0.03875217981011432 0.0 29 0.0 0.0 0.0 0.03875217981011432 0.0 30 0.0 0.0 0.0 0.04521087644513337 0.0 31 0.0 0.0 0.0 0.05166957308015242 0.0 32 0.0 0.0 0.0 0.05166957308015242 0.0 33 0.0 0.0 0.0 0.05812826971517148 0.0 34 0.0 0.0 0.0 0.07104566298520959 0.0 35 0.0 0.0 0.0 0.07750435962022864 0.0 36 0.0 0.0 0.0 0.07750435962022864 0.0 37 0.0 0.0 0.0 0.0968804495252858 0.0 38 0.0 0.0 0.0 0.10333914616030485 0.0 39 0.0 0.0 0.0 0.10979784279532391 0.0 40 0.0 0.0 0.0 0.12917393270038105 0.0 41 0.0 0.0 0.0 0.14209132597041918 0.0 42 0.0 0.0 0.0 0.14855002260543823 0.0 43 0.0 0.0 0.0 0.15500871924045728 0.0 44 0.0 0.0 0.0 0.16146741587547633 0.0 45 0.0 0.0 0.0 0.17438480914551444 0.0 46 0.0 0.0 0.0 0.1808435057805335 0.0 47 0.0 0.0 0.0 0.18730220241555254 0.0 48 0.0 0.0 0.0 0.1937608990505716 0.0 49 0.0 0.0 0.0 0.21313698895562874 0.0 50 0.0 0.0 0.0 0.21959568559064782 0.0 51 0.0 0.0 0.0 0.23897177549570497 0.0 52 0.0 0.0 0.0 0.2518891687657431 0.0 53 0.0 0.0 0.0 0.27126525867080026 0.0 54 0.0 0.0 0.0 0.28418265194083836 0.0 55 0.0 0.0 0.0 0.2906413485758574 0.0 56 0.0 0.0 0.0 0.3035587418458955 0.0 57 0.0 0.0 0.0 0.3164761351159336 0.0 58 0.0 0.0 0.0 0.3293935283859717 0.0 59 0.0 0.0 0.0 0.34876961829102887 0.0 60 0.0 0.0 0.0 0.3875217981011432 0.0 61 0.0 0.0 0.0 0.39398049473616226 0.0 62 0.0 0.0 0.0 0.4133565846412194 0.0 63 0.0 0.0 0.0 0.43273267454627656 0.0 64 0.0 0.0 0.0 0.43919137118129564 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACA 95 0.0 70.00001 1 TTTACAC 95 0.0 70.00001 2 CGCGACT 20 7.684063E-5 70.0 23 GCGACTT 20 7.684063E-5 70.0 24 CACGCGA 20 7.684063E-5 70.0 21 CCACGCG 20 7.684063E-5 70.0 20 TTATTAC 15 0.002183478 70.0 2 CCCACGC 20 7.684063E-5 70.0 19 ATTACAC 15 0.002183478 70.0 4 CTTATTA 15 0.002183478 70.0 1 TATTACA 15 0.002183478 70.0 3 TCTTTAT 30 9.590076E-8 70.0 1 ACGCGAC 20 7.684063E-5 70.0 22 CGACTTG 20 7.684063E-5 70.0 25 TCTTAAT 15 0.002183478 70.0 1 AACATAT 15 0.002183478 70.0 67 CTTTATA 35 2.7870556E-7 60.000004 2 TTTATAC 35 2.7870556E-7 60.000004 3 CTATACA 100 0.0 59.500004 1 CTTATAC 695 0.0 59.42446 1 >>END_MODULE