FastQCFastQC Report
Wed 25 May 2016
SRR1780441_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780441_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68235
Sequences flagged as poor quality0
Sequence length50
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG890.13043159668791676No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGCG207.8365416E-443.9633458
TTGTATA207.8365416E-443.96334544
TCTACAC207.8365416E-443.9633453
TTTACAC454.6929927E-1043.963342
AAAGGAC301.2834858E-436.6899151
AAAGCAA301.2834858E-436.6899151
CTGCGTG301.294635E-436.6361232
CTTTACA607.728886E-933.0209241
GCTGCGT401.8054086E-532.97250731
AAGGACA353.2019874E-431.402392
CTATACA453.9967694E-529.3519331
CTTCTAC300.005689859429.3519331
ACAGGCG300.005730867429.3088955
CTTATAC3800.027.8070951
TTATACA3750.027.5503622
CTGTGTA406.967887E-427.49724834
TGACGCC508.1522034E-526.4361513
AAGGGGC756.769369E-826.416741
TTACACA756.866503E-826.3780063
TATACAC4000.025.2789213
Produced by FastQC (version 0.11.5)