FastQCFastQC Report
Wed 25 May 2016
SRR1780441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780441_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68235
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT2797540.99802154319631No Hit
ATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTT1735125.428299259910602Illumina PCR Primer Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC48237.068220121638455No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG39185.741921301384919No Hit
CTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC27924.091741774749029No Hit
TATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT15542.277423609584524No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC9341.3687990034439803No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC8951.3116435846706236No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG6210.9100901296988348No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC5410.7928482450355389No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG5010.734227302703891No Hit
TTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC4980.7298307320290174No Hit
CTTAACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC4580.6712097896973693No Hit
ACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTTCT3500.5129332454019199Illumina PCR Primer Index 10 (95% over 23bp)
CTTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG3270.47922620356122225No Hit
CTTATTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG3200.46896753865318386No Hit
CTTATCACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTC2620.38396717227229427No Hit
CTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCT2250.3297428006155199No Hit
ATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGTCTTCTGCTT1810.2652597640507071Illumina PCR Primer Index 10 (96% over 27bp)
TCTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT1360.19931120392760313No Hit
TCTTTACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCGT1190.17439730343665275No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGC1180.17293177987836153No Hit
CCTTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG1000.14655235582911996No Hit
CTTATATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCC890.13043159668791676No Hit
CTCTATACACATCTCCGAGCCCACGAGACTGTGCCTTATCTCGTATGCCG730.10698321975525756No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAT207.749438E-444.0631451
TCTTAAT502.7284841E-1144.0631451
CTTATTA351.403514E-744.063141
CTTACAC351.403514E-744.063141
CTTATAA600.044.063141
TCTTTAT1650.044.063141
TATATAC207.805721E-443.9985353
TTATTAC351.4198486E-743.9985352
TTATATA207.805721E-443.9985352
ATTACAC351.4198486E-743.9985354
TCTGCTT351.4198486E-743.99853544
TATTACA351.4198486E-743.9985353
ATATACA207.805721E-443.9985354
TATAACA600.043.998533
TTATAAC600.043.998532
ATAACAC600.043.998534
CTTTACA4850.043.6088871
CTATACA2200.042.0602721
CCGTCTT18050.042.04846244
TTTACAC5050.041.820392
Produced by FastQC (version 0.11.5)