FastQCFastQC Report
Wed 25 May 2016
SRR1780440_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780440_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64655
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTC252.8127924E-669.9914968
ACGTCGC207.876985E-569.9914970
GGATCCT150.002216461869.99148626
GACGTCG308.297331E-658.3262469
TGTGACC252.2772214E-456.4736371
GTGACCA252.3755658E-455.9931952
GGACTAG200.00669551352.944031
GACAGAG200.00692587452.493627
TAAAGGT200.00692587452.493625
AGCTTTA200.00692587452.4936224
CCAATAG200.00692587452.4936220
TGAGTTT200.00692587452.4936235
TTGAGTT200.00692587452.4936234
CTACAGT200.00692587452.4936248
TACGTGA200.00692587452.4936236
ACCGCCT305.8416015E-446.6609961
GTAAGGA305.8416015E-446.660999
ATAGTGT404.5498935E-543.7446824
ATCTCGG501.6939368E-434.99574741
TGTAAGG400.002404074234.9957478
Produced by FastQC (version 0.11.5)