##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780440_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64655 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.84155904415745 34.0 23.0 34.0 12.0 34.0 2 28.967442579846878 34.0 23.0 34.0 11.0 34.0 3 29.18976103936277 34.0 27.0 34.0 11.0 34.0 4 29.417430979815947 34.0 31.0 34.0 11.0 34.0 5 29.669089784239425 34.0 31.0 34.0 11.0 34.0 6 32.186281030082746 37.0 31.0 38.0 11.0 38.0 7 31.435248627329674 37.0 27.0 38.0 11.0 38.0 8 30.981826618204316 36.0 27.0 38.0 11.0 38.0 9 31.433887557033486 37.0 27.0 38.0 12.0 38.0 10-11 31.571100456267885 37.0 27.5 38.0 11.0 38.0 12-13 31.881942618513648 37.0 29.5 38.0 11.0 38.0 14-15 31.54517052045472 37.0 27.0 38.0 11.0 38.0 16-17 31.300371200989872 37.0 27.0 38.0 11.0 38.0 18-19 31.337939834506226 37.0 26.5 38.0 11.0 38.0 20-21 31.44984146624391 37.0 27.0 38.0 11.0 38.0 22-23 31.329572345526255 37.0 27.0 38.0 11.0 38.0 24-25 31.708174155131083 37.0 27.5 38.0 11.0 38.0 26-27 31.688276235403293 37.0 27.0 38.0 11.0 38.0 28-29 31.69191091176243 37.0 27.0 38.0 11.0 38.0 30-31 31.565006573350864 37.0 27.0 38.0 11.0 38.0 32-33 31.437707833887558 37.0 27.0 38.0 11.0 38.0 34-35 31.351434537158767 37.0 27.0 38.0 11.0 38.0 36-37 31.315056840151573 37.0 26.5 38.0 11.0 38.0 38-39 31.65655401747738 37.0 27.0 38.0 11.0 38.0 40-41 31.25848735596628 37.0 27.0 38.0 11.0 38.0 42-43 31.541489443971848 37.0 27.0 38.0 11.0 38.0 44-45 30.79092877581007 37.0 26.0 38.0 11.0 38.0 46-47 31.387734900626402 37.0 27.0 38.0 11.0 38.0 48-49 31.258386822364862 37.0 27.0 38.0 11.0 38.0 50-51 31.222279792746114 37.0 26.5 38.0 11.0 38.0 52-53 31.187935967829247 37.0 27.0 38.0 11.0 38.0 54-55 30.9558193488516 37.0 26.0 38.0 11.0 38.0 56-57 30.911878431675817 37.0 26.0 38.0 11.0 38.0 58-59 30.86964658572423 36.5 26.5 38.0 11.0 38.0 60-61 30.930167813780837 37.0 26.0 38.0 11.0 38.0 62-63 30.926030469414584 37.0 26.0 38.0 11.0 38.0 64-65 30.950924135797695 37.0 26.0 38.0 11.0 38.0 66-67 31.000835202227208 37.0 26.0 38.0 11.0 38.0 68-69 30.52718273915397 36.0 24.5 38.0 11.0 38.0 70-71 30.36997911994432 36.0 24.5 38.0 11.0 38.0 72-73 30.391361843631586 36.0 24.5 38.0 11.0 38.0 74-75 30.015304307478154 36.0 24.0 38.0 11.0 38.0 76 29.136803031474752 34.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.8875622511657468 1101 2 -0.6558886456844313 1101 3 -0.4733345680309071 1101 4 -1.2048823728899443 1101 5 -1.93556906113011 1101 6 -3.3120823566592037 1101 7 -1.5503030072749127 1101 8 -1.5091567623565432 1101 9 -0.8450913741159276 1101 10-11 -1.4911992042260422 1101 12-13 -0.8939673466798261 1101 14-15 -0.3722185293528568 1101 16-17 -2.075357974363829 1101 18-19 -1.1307255468229265 1101 20-21 -1.9947518115315077 1101 22-23 -0.1900930667110572 1101 24-25 -1.4813024557886685 1101 26-27 -1.368068384844964 1101 28-29 -0.5275037095107713 1101 30-31 -1.0193235214942362 1101 32-33 -1.0472851374397933 1101 34-35 -0.912432152343623 1101 36-37 -0.38150812172643 1101 38-39 -0.8563916836149481 1101 40-41 -0.1334073216722622 1101 42-43 -1.1655390643908135 1101 44-45 -0.2685824216295316 1101 46-47 -0.35703861625220057 1101 48-49 -0.2906519429790002 1101 50-51 -0.3212828264880905 1101 52-53 -0.48671766447266407 1101 54-55 -1.5247703140217368 1101 56-57 -1.3305585994757259 1101 58-59 -0.4420276120412119 1101 60-61 -0.9906702924647774 1101 62-63 -1.235779176598296 1101 64-65 -0.8518758222522429 1101 66-67 -1.1911107496347668 1101 68-69 -0.7496511915215365 1101 70-71 0.1581037249369679 1101 72-73 -1.3669300964850883 1101 74-75 -1.4548285138511332 1101 76 -1.1907856630999198 1102 1 -0.4291650891888068 1102 2 -1.705070295285335 1102 3 0.18062012035093744 1102 4 -0.10160090872804872 1102 5 -0.21816198219759286 1102 6 0.4512813456708784 1102 7 0.92587211164793 1102 8 0.8244721983787642 1102 9 -0.22298381949204327 1102 10-11 -0.37638827138721354 1102 12-13 -4.043568249687162 1102 14-15 -1.85917732964311 1102 16-17 -2.551976534389631 1102 18-19 -0.4764886686482903 1102 20-21 -0.3486178116927583 1102 22-23 -1.2244597521903735 1102 24-25 -0.3018297475729135 1102 26-27 -6.006099506347827 1102 28-29 -0.8262822308362558 1102 30-31 -0.732638848431268 1102 32-33 -5.907722128490345 1102 34-35 -1.3370270084268334 1102 36-37 -0.5544098390528838 1102 38-39 -0.4668488310792682 1102 40-41 -0.9921914867936046 1102 42-43 -0.7747062013737995 1102 44-45 -5.6065046984980285 1102 46-47 -1.309026038650007 1102 48-49 -1.2690442674166391 1102 50-51 -5.26772723509891 1102 52-53 -1.7245431905550603 1102 54-55 0.10235618763108789 1102 56-57 -1.6677955582760156 1102 58-59 0.2710256815168002 1102 60-61 -0.5042758717601075 1102 62-63 -3.7383390411471957 1102 64-65 -1.3771318087071336 1102 66-67 -0.9826329620027394 1102 68-69 -5.7832922461051055 1102 70-71 -0.9183092396683712 1102 72-73 -0.10687204601342515 1102 74-75 -5.4455968763440765 1102 76 -3.210135820319948 1103 1 -0.3892500148788294 1103 2 -0.6399252138982163 1103 3 -1.1219984217580503 1103 4 -0.9034055796409994 1103 5 -0.35736934242406093 1103 6 -1.4268502891486534 1103 7 -0.2653522337165448 1103 8 0.3666519577559768 1103 9 0.20068921660137207 1103 10-11 0.1918176734110908 1103 12-13 -1.5260700189723693 1103 14-15 -1.3502424393387926 1103 16-17 -0.9684662725354194 1103 18-19 -0.4820265313517602 1103 20-21 -0.6461231195483883 1103 22-23 -1.1278567797912231 1103 24-25 -0.6474065345509707 1103 26-27 -0.4805156422289265 1103 28-29 0.1696130274793859 1103 30-31 -0.6177060812692012 1103 32-33 -4.767010875836419 1103 34-35 -0.6232619695025541 1103 36-37 0.4418392763045098 1103 38-39 0.5381582460615633 1103 40-41 -0.3487378420660754 1103 42-43 0.31259033082719156 1103 44-45 -3.992562731052878 1103 46-47 0.14387558909238507 1103 48-49 0.2947207715076381 1103 50-51 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32-33 -1.638235553552807 1104 34-35 -0.4471723439654163 1104 36-37 -0.6945020373848685 1104 38-39 -1.2936250565780938 1104 40-41 -0.38992875673765326 1104 42-43 -0.5152488343047921 1104 44-45 -3.5379893731645993 1104 46-47 -0.9806486589125285 1104 48-49 -0.11952109466793104 1104 50-51 -0.0029913375727801395 1104 52-53 -0.7530824452265605 1104 54-55 -1.4311448856701041 1104 56-57 -0.3788312059517054 1104 58-59 0.9345721501799247 1104 60-61 -0.21371036519713726 1104 62-63 -0.38912906120561175 1104 64-65 0.5083416754748704 1104 66-67 0.1717992440710887 1104 68-69 -3.208337113613993 1104 70-71 -0.027381274014008028 1104 72-73 0.5470499021162141 1104 74-75 -1.111377922900715 1104 76 1.1123823906101293 1105 1 -0.765491149630602 1105 2 -0.024795190257552946 1105 3 -0.915497239724953 1105 4 -0.2764552250310679 1105 5 -0.3237996024713432 1105 6 0.2174759519860956 1105 7 0.5306288537539032 1105 8 0.07918150858339956 1105 9 0.5850863797219432 1105 10-11 -0.2319411918371408 1105 12-13 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-1.338981192321576 1106 1 -0.046392872021684184 1106 2 0.6251541511811496 1106 3 -0.5192206439802725 1106 4 -0.021262722498139652 1106 5 0.4791385940838744 1106 6 0.4052925679942021 1106 7 1.8390128456485364 1106 8 1.2837154498194714 1106 9 1.484022549934707 1106 10-11 0.3555081496015653 1106 12-13 0.2808744254720743 1106 14-15 0.4582893066929543 1106 16-17 1.0305416639725173 1106 18-19 -0.06615351547874226 1106 20-21 0.2943530709278015 1106 22-23 0.23226226782782433 1106 24-25 1.0736252114807776 1106 26-27 0.52007959586631 1106 28-29 -0.45558538521902747 1106 30-31 -0.11215052571364836 1106 32-33 1.2734653146397683 1106 34-35 0.44836501462442513 1106 36-37 0.49620435566959387 1106 38-39 0.06871380161711826 1106 40-41 0.2284446976164638 1106 42-43 0.958820489557354 1106 44-45 0.7256912372010902 1106 46-47 0.47701050972730386 1106 48-49 0.15920489849176533 1106 50-51 0.06215599207730804 1106 52-53 -0.8326338790597632 1106 54-55 0.12053559315125639 1106 56-57 1.2793344083603166 1106 58-59 -0.40032327207737595 1106 60-61 0.9727866092832649 1106 62-63 0.6631433671027303 1106 64-65 0.2918854797369086 1106 66-67 1.4766861159469116 1106 68-69 0.7641372352415736 1106 70-71 0.6638677394035106 1106 72-73 0.11050359851190095 1106 74-75 -1.1184813167444432 1106 76 -1.0337633605079972 1107 1 -0.4684821265716721 1107 2 0.008038718620689167 1107 3 0.7353605078521888 1107 4 0.19772880081275446 1107 5 0.48387556740723525 1107 6 0.26913574924576267 1107 7 1.4546012275981717 1107 8 1.3624053027239817 1107 9 0.5259073666886351 1107 10-11 1.145788004999222 1107 12-13 0.7190288757338514 1107 14-15 0.5986377235466094 1107 16-17 1.1883109235087908 1107 18-19 1.3556552603876248 1107 20-21 1.1433620462456808 1107 22-23 -0.10342397001810255 1107 24-25 0.6111158621913226 1107 26-27 0.776639680633199 1107 28-29 -0.4831502069592446 1107 30-31 0.7041671008134109 1107 32-33 1.6800659129942979 1107 34-35 1.112834598269405 1107 36-37 0.5598730168513377 1107 38-39 0.7262181646188637 1107 40-41 0.9208374924307243 1107 42-43 0.6988323319906584 1107 44-45 1.5051103346830175 1107 46-47 0.7568191609364838 1107 48-49 1.07348749634766 1107 50-51 1.3830547704368215 1107 52-53 1.5901098257520019 1107 54-55 0.644641177793492 1107 56-57 0.9140732515415699 1107 58-59 1.2211707443773712 1107 60-61 0.6532590600436734 1107 62-63 0.8964860497255174 1107 64-65 0.6185496532597163 1107 66-67 1.4993425607237754 1107 68-69 2.19168024197306 1107 70-71 0.8250962360377692 1107 72-73 1.5971496967417664 1107 74-75 0.9570609420438885 1107 76 -0.16328218374409786 1108 1 -1.5029755050749074 1108 2 -1.0092359685030878 1108 3 -1.5506377562797375 1108 4 -1.10456422491729 1108 5 -0.6968226995244784 1108 6 -0.6949429807707581 1108 7 -0.5611335765567098 1108 8 -0.899598784965356 1108 9 -0.569453385894171 1108 10-11 -0.11009854762277982 1108 12-13 -0.802347499661618 1108 14-15 -0.32877779287414555 1108 16-17 -1.2836772053934133 1108 18-19 -1.1159688961764793 1108 20-21 -0.27468818193697686 1108 22-23 -0.936234676404414 1108 24-25 -1.6531700520791937 1108 26-27 -1.2773962126389087 1108 28-29 -0.8652651662223398 1108 30-31 -0.28015409077603337 1108 32-33 0.25789999760386806 1108 34-35 -0.8927509772268571 1108 36-37 -0.48727539986898805 1108 38-39 -0.15633076166273696 1108 40-41 -0.49413296981414234 1108 42-43 -0.09314347784779287 1108 44-45 -0.3351058088900736 1108 46-47 -0.33084217680185546 1108 48-49 0.22603390282076674 1108 50-51 -0.4487620114877515 1108 52-53 -0.6801956310332784 1108 54-55 -0.2563583462426813 1108 56-57 0.4097615792286682 1108 58-59 -1.8780257166915888 1108 60-61 -0.862615733472019 1108 62-63 -1.4381585172873876 1108 64-65 -0.2262581413478877 1108 66-67 -0.11463677684657014 1108 68-69 -0.018807760938717166 1108 70-71 0.25119585246691756 1108 72-73 -0.36716701518812656 1108 74-75 -0.08333998748546634 1108 76 -0.8640409260796815 1109 1 -0.152212977841792 1109 2 -0.18304690701991078 1109 3 0.5753560755964457 1109 4 -0.47827330450872196 1109 5 0.39448250942778884 1109 6 0.7816153193169555 1109 7 -0.4791088474731602 1109 8 1.0581863492903665 1109 9 1.2882553541675108 1109 10-11 0.48612984272325477 1109 12-13 0.26533209742974506 1109 14-15 0.27343480933845754 1109 16-17 -0.3361911402602864 1109 18-19 0.16202108769585877 1109 20-21 0.3876070391817734 1109 22-23 0.30071464878020393 1109 24-25 0.8256093384649041 1109 26-27 1.2525531408398543 1109 28-29 0.5420997999661523 1109 30-31 0.22250555894244073 1109 32-33 0.8111637273381795 1109 34-35 0.4526639564233683 1109 36-37 0.6397228741881058 1109 38-39 1.3901423730456912 1109 40-41 0.5740134806852488 1109 42-43 -0.24587527763841877 1109 44-45 0.388389649899505 1109 46-47 -0.03522494000814547 1109 48-49 0.16350384029070852 1109 50-51 0.9318649867863016 1109 52-53 1.2725248510989715 1109 54-55 0.38938479950046556 1109 56-57 1.2373370538629622 1109 58-59 0.01601270675860178 1109 60-61 0.8649823764790341 1109 62-63 -0.09843070697134593 1109 64-65 0.5418854797369086 1109 66-67 0.33978135404214527 1109 68-69 1.5822589283632666 1109 70-71 -0.15458464154886897 1109 72-73 -0.23043555492725432 1109 74-75 1.3259631276999997 1109 76 0.34454351779887915 1110 1 -0.8452783836731541 1110 2 -0.030114339193723083 1110 3 -0.7133695801504842 1110 4 -0.0902850122651131 1110 5 -0.8982676875777251 1110 6 -0.2027368139713488 1110 7 0.849777789924115 1110 8 0.06322406177488915 1110 9 -0.8404455351716749 1110 10-11 -1.2478986386456477 1110 12-13 -0.28694472346409583 1110 14-15 0.14148332898272287 1110 16-17 -0.5707121354195799 1110 18-19 0.03706330316360962 1110 20-21 -0.5441727649384553 1110 22-23 -0.5551617443302312 1110 24-25 -0.4005389222578266 1110 26-27 -1.779629117406234 1110 28-29 -0.7495950103456757 1110 30-31 -0.9915831498271217 1110 32-33 -0.09697541484350936 1110 34-35 0.23832195484732566 1110 36-37 -0.6003002036009271 1110 38-39 -0.15025782958855416 1110 40-41 -0.1393997806003533 1110 42-43 0.04550995257660162 1110 44-45 0.5112788765121365 1110 46-47 0.2858377640332783 1110 48-49 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2116 36-37 0.4399874244526565 2116 38-39 0.17056565346896946 2116 40-41 0.5938547505265142 2116 42-43 0.955182923419784 2116 44-45 1.6216347998113179 2116 46-47 0.9012829964997913 2116 48-49 0.9157084258286261 2116 50-51 0.5575263624476818 2116 52-53 1.314852893427009 2116 54-55 0.5040232474722472 2116 56-57 -0.4491708826449745 2116 58-59 -1.1660860586734962 2116 60-61 -0.8175573060606496 2116 62-63 0.7309343723937367 2116 64-65 0.8417091128938701 2116 66-67 0.8896049871991139 2116 68-69 0.6986610447653803 2116 70-71 -2.3728386098028373 2116 72-73 0.3193880782297107 2116 74-75 -1.842467207397 2116 76 -0.27009493017290254 2117 1 0.8446821208497148 2117 2 1.2392830362681195 2117 3 1.3348212721873907 2117 4 1.4062750590815547 2117 5 0.20996266023728083 2117 6 0.013469072128792448 2117 7 -0.148120031454404 2117 8 -1.0146521007935903 2117 9 1.7594716517311113 2117 10-11 1.1541430227124856 2117 12-13 1.491578018286443 2117 14-15 -5.252071365262623E-4 2117 16-17 0.48728888381853963 2117 18-19 1.0073779929330051 2117 20-21 0.24484494432386938 2117 22-23 1.2144585894702509 2117 24-25 1.6348955279906114 2117 26-27 1.8926380503858375 2117 28-29 1.9046762343988775 2117 30-31 1.061003166901127 2117 32-33 1.9275500024070986 2117 34-35 0.3297985760729567 2117 36-37 -0.07126784279063259 2117 38-39 1.5063385521161266 2117 40-41 -0.04680836768151053 2117 42-43 0.045557729363451926 2117 44-45 1.3181491784614252 2117 46-47 1.2786393948142738 2117 48-49 0.41275137696339215 2117 50-51 1.2824653733148352 2117 52-53 1.8848240622005719 2117 54-55 0.30978210013728713 2117 56-57 1.2683385429482819 2117 58-59 0.8092041017464027 2117 60-61 1.7292379922316528 2117 62-63 0.9804908150639484 2117 64-65 1.5444517757163716 2117 66-67 0.08386461608948181 2117 68-69 1.1757069529490174 2117 70-71 1.90344216198692 2117 72-73 2.1123392129972167 2117 74-75 1.32634331969696 2117 76 1.104674050384503 2118 1 0.8894249333613367 2118 2 1.717804254700816 2118 3 1.2905619647153799 2118 4 1.0892445238786692 2118 5 0.7110226520548899 2118 6 1.9205617191329196 2118 7 1.7461039429570242 2118 8 -2.8764812375511326 2118 9 -0.0563501208727466 2118 10-11 0.8375216071585037 2118 12-13 2.146169456527975 2118 14-15 0.40329448267639023 2118 16-17 1.1823273782582326 2118 18-19 0.5857615983928959 2118 20-21 -1.605491649569089 2118 22-23 0.5533026404986323 2118 24-25 1.111668689741638 2118 26-27 1.5233145855143384 2118 28-29 1.1943887348637858 2118 30-31 1.2475803231476341 2118 32-33 0.9729994761304503 2118 34-35 1.8421279545830167 2118 36-37 1.9379745420049979 2118 38-39 1.628237611140925 2118 40-41 0.39135877629140836 2118 42-43 1.0917138642985549 2118 44-45 1.7355256057311124 2118 46-47 0.9248624766010032 2118 48-49 -0.2886470683819411 2118 50-51 1.8077564061559812 2118 52-53 -0.19158833040551926 2118 54-55 1.2399714109566453 2118 56-57 2.24918430484065 2118 58-59 1.6023941192269682 2118 60-61 -0.727265150551105 2118 62-63 0.7810263898770593 2118 64-65 0.5356742996126833 2118 66-67 -1.4190178178005013 2118 68-69 2.3953484153657953 2118 70-71 1.074193826360034 2118 72-73 -0.8389011633928618 2118 74-75 0.7759286211437555 2118 76 0.6981206974630147 2119 1 1.5427319671031547 2119 2 1.0656303416573394 2119 3 1.0314550316954048 2119 4 0.6610088347734155 2119 5 0.9788168437621252 2119 6 0.6371136748153106 2119 7 -1.5462831646474768 2119 8 1.3948801859842028 2119 9 0.2745630904752474 2119 10-11 0.18618704278045684 2119 12-13 0.671077128174776 2119 14-15 -0.36225445135080037 2119 16-17 -0.3881359422050892 2119 18-19 -0.1736181229433491 2119 20-21 -0.2823843808096491 2119 22-23 1.4048008778664318 2119 24-25 0.45634721920277954 2119 26-27 0.921060934347647 2119 28-29 0.914507922374284 2119 30-31 0.5286152400521225 2119 32-33 0.7609170520915107 2119 34-35 0.8638251175845291 2119 36-37 -0.17512769066245681 2119 38-39 0.1475426304459475 2119 40-41 0.47949038616215134 2119 42-43 -0.17494720671034614 2119 44-45 -0.7992444127345593 2119 46-47 0.05093264614943749 2119 48-49 0.17842948021634797 2119 50-51 1.1150839200052403 2119 52-53 -0.9791911506170337 2119 54-55 -0.4701175600018921 2119 56-57 1.6663196078455194 2119 58-59 1.7065782723241618 2119 60-61 0.9900002514969088 2119 62-63 -0.32036692890756413 2119 64-65 0.609935672787099 2119 66-67 0.2805792948400878 2119 68-69 -1.4308434597391226 2119 70-71 -0.1491148860791114 2119 72-73 -1.88185483134653 2119 74-75 -0.4818983051614296 2119 76 0.8471890204443824 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 36.0 13 275.0 14 716.0 15 1203.0 16 1453.0 17 1840.0 18 1974.0 19 1935.0 20 1721.0 21 1303.0 22 1084.0 23 967.0 24 886.0 25 835.0 26 853.0 27 1021.0 28 1100.0 29 1334.0 30 1575.0 31 1958.0 32 2425.0 33 3219.0 34 4421.0 35 6550.0 36 11029.0 37 12938.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.1052541342602 22.076403933976525 15.441325457067597 29.377016474695676 2 20.36501430670482 22.38032634753693 33.463769236717965 23.79089010904029 3 15.689428505142681 24.307478153275074 33.72360992962648 26.279483411955766 4 14.937746500657333 20.405227747273994 35.290387441033175 29.366638311035498 5 11.259763359368959 38.98847730260614 32.673420462454565 17.078338875570335 6 22.530353414275773 36.734977959941226 17.567086845564923 23.16758178021808 7 21.6858711623231 31.098909597092263 20.921815791508777 26.293403449075864 8 19.279251411337096 38.46415590441575 18.521382723687264 23.735209960559896 9 20.84293558116155 20.822828860876964 20.188693836516897 38.145541721444594 10-11 21.305390147707058 27.198205861882297 26.218389915706442 25.278014074704195 12-13 20.746268656716417 24.954759879359678 25.908282422086458 28.390689041837447 14-15 19.85461294563452 26.86721831258217 22.9031010749362 30.375067666847112 16-17 21.262083365555643 28.18962183899157 23.97185059160158 26.576444203851214 18-19 19.672105792282114 27.542340112906967 24.99342664913773 27.792127445673188 20-21 19.182584486891965 27.689273838063567 25.15505374680999 27.973087928234474 22-23 21.552084138891036 27.72562060165494 24.56267883381022 26.1596164256438 24-25 20.363467635913697 29.21738457969221 24.079344211584562 26.33980357280953 26-27 18.944397185059163 29.64967906581084 25.47830794215451 25.927615806975485 28-29 20.33717423246462 27.438713169901785 27.70938055834816 24.514732039285438 30-31 20.27840074240198 27.584873559662825 27.25697935194494 24.879746345990256 32-33 18.93666383110355 29.460985229293946 26.171989791972777 25.430361147629725 34-35 20.085840228907276 28.271595390921046 26.132549686799166 25.51001469337252 36-37 19.98298662129766 27.8454875879669 26.57721753924677 25.594308251488673 38-39 19.669785786095428 27.091485577294872 27.908127755007346 25.33060088160235 40-41 19.335704895213055 26.765138040368107 27.334312891501046 26.564844172917795 42-43 18.280875415667776 25.952362539633437 28.59716959245225 27.169592452246537 44-45 17.989327971541258 26.506844018250714 28.836130229680613 26.667697780527416 46-47 18.39532905421081 27.38225968602583 27.9746345990256 26.24777666073776 48-49 17.885701028536076 27.826154203077873 27.607300286134095 26.680844482251953 50-51 17.659887093032246 27.567860180960484 26.8347382259686 27.937514500038667 52-53 17.36833964890573 27.480473281262086 27.409326424870468 27.74186064496172 54-55 16.89815172840461 27.815327507540022 26.821591524244067 28.464929239811305 56-57 17.560900162400433 27.625087000232 26.670791122109662 28.14322171525791 58-59 17.764287371432992 27.182739153971074 26.287990101306935 28.764983373288995 60-61 17.29487278632743 26.667697780527416 26.970071920191785 29.067357512953368 62-63 17.519913386435697 27.09071224189931 26.549377465006575 28.839996906658417 64-65 18.175701801871472 27.82383419689119 25.05838682236486 28.942077178872477 66-67 17.77975407934421 28.48271595390921 24.31985151960405 29.417678447142524 68-69 17.959167891114376 28.314902173072458 24.50235867295646 29.2235712628567 70-71 18.43940917175779 28.028768076714872 24.49307864820973 29.038744103317608 72-73 18.654396411723763 26.96543190781842 25.06689351171603 29.313278168741785 74-75 19.344211584564224 26.837058232155286 24.45673188461836 29.36199829866213 76 19.40607841620911 25.41798778130075 25.520068053514812 29.65586574897533 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.0 11 1.5 12 3.0 13 3.0 14 5.5 15 5.5 16 9.0 17 15.0 18 13.0 19 18.5 20 26.5 21 27.0 22 37.5 23 56.5 24 94.5 25 124.0 26 151.5 27 213.5 28 298.5 29 349.0 30 426.5 31 545.0 32 755.0 33 924.0 34 1022.0 35 1366.0 36 1739.0 37 1866.0 38 2121.0 39 2667.5 40 3235.0 41 3682.0 42 3853.0 43 3984.5 44 4308.0 45 4484.0 46 4468.0 47 4554.0 48 4477.5 49 4167.0 50 4019.0 51 3807.0 52 3286.5 53 2767.0 54 2556.0 55 2423.0 56 2012.5 57 1574.0 58 1413.0 59 1190.5 60 860.0 61 678.0 62 604.0 63 474.0 64 318.5 65 232.5 66 147.5 67 123.0 68 104.0 69 74.0 70 46.5 71 30.0 72 28.0 73 26.5 74 24.0 75 21.0 76 17.5 77 12.5 78 10.0 79 9.0 80 8.0 81 6.0 82 7.5 83 10.0 84 6.0 85 3.0 86 3.5 87 3.0 88 4.0 89 4.5 90 3.0 91 2.5 92 3.0 93 3.5 94 3.0 95 2.0 96 2.0 97 1.5 98 2.5 99 6.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7671487123965663 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 64655.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.15304307478154 #Duplication Level Percentage of deduplicated Percentage of total 1 82.33197113294496 58.58170288454102 2 9.077471524215285 12.917794447451861 3 3.814885662116338 8.143221715257907 4 1.8367968002782367 5.227747273992731 5 1.002086775063038 3.5650761735364624 6 0.6564646552473699 2.8025674735132626 7 0.3825754282236327 1.905498414662439 8 0.2738892270237371 1.5590441574510865 9 0.1934614381358143 1.2388833036888098 >10 0.43039735675158686 4.0584641559044154 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0015466707911221097 0.0 6 0.0 0.0 0.0 0.0015466707911221097 0.0 7 0.0 0.0 0.0 0.0030933415822442193 0.0 8 0.0 0.0 0.0 0.0030933415822442193 0.0 9 0.0 0.0 0.0 0.004640012373366329 0.0 10 0.0 0.0 0.0 0.007733353955610548 0.0 11 0.0 0.0 0.0 0.009280024746732658 0.0 12 0.0 0.0 0.0 0.010826695537854768 0.0 13 0.0 0.0 0.0 0.013920037120098988 0.0 14 0.0 0.0 0.0 0.015466707911221097 0.0 15 0.0 0.0 0.0 0.017013378702343207 0.0 16 0.0 0.0 0.0015466707911221097 0.017013378702343207 0.0 17 0.0 0.0 0.0015466707911221097 0.018560049493465316 0.0 18 0.0 0.0 0.0015466707911221097 0.021653391075709537 0.0 19 0.0 0.0 0.0015466707911221097 0.023200061866831646 0.0 20 0.0 0.0 0.0015466707911221097 0.023200061866831646 0.0 21 0.0 0.0 0.0015466707911221097 0.024746732657953754 0.0 22 0.0 0.0 0.0015466707911221097 0.030933415822442193 0.0 23 0.0 0.0 0.0015466707911221097 0.034026757404686414 0.0 24 0.0 0.0 0.0015466707911221097 0.034026757404686414 0.0 25 0.0 0.0 0.0015466707911221097 0.034026757404686414 0.0 26 0.0 0.0 0.0015466707911221097 0.03557342819580852 0.0 27 0.0 0.0 0.0015466707911221097 0.052586806898151726 0.0 28 0.0 0.0 0.0015466707911221097 0.07733353955610549 0.0 29 0.0 0.0 0.0015466707911221097 0.0958935890495708 0.0 30 0.0 0.0 0.0015466707911221097 0.12373366328976877 0.0 31 0.0 0.0 0.0015466707911221097 0.1562137499033331 0.0 32 0.0 0.0 0.0015466707911221097 0.21498723996597324 0.0 33 0.0 0.0 0.0015466707911221097 0.26602737607300286 0.0 34 0.0 0.0 0.0015466707911221097 0.32789420771788724 0.0 35 0.0 0.0 0.0015466707911221097 0.4191477843940917 0.0 36 0.0 0.0 0.0015466707911221097 0.5815482174619132 0.0 37 0.0 0.0 0.0015466707911221097 0.8460289227437939 0.0 38 0.0 0.0 0.0015466707911221097 1.1460830562214832 0.0 39 0.0 0.0 0.0015466707911221097 1.4786172763127368 0.0 40 0.0 0.0 0.0015466707911221097 1.9302451473203928 0.0 41 0.0 0.0 0.0015466707911221097 2.4468331915551773 0.0 42 0.0 0.0 0.0015466707911221097 3.077874874332998 0.0 43 0.0 0.0 0.0015466707911221097 3.611476297270126 0.0 44 0.0 0.0 0.0015466707911221097 4.2347846260923365 0.0 45 0.0 0.0 0.0015466707911221097 4.899853066274844 0.0 46 0.0 0.0 0.0015466707911221097 5.52780140747042 0.0 47 0.0 0.0 0.0015466707911221097 6.175856468950584 0.0 48 0.0 0.0 0.0015466707911221097 6.726471270590055 0.0 49 0.0 0.0 0.0015466707911221097 7.184285824762199 0.0 50 0.0 0.0 0.0015466707911221097 7.699327198205862 0.0 51 0.0 0.0 0.0015466707911221097 8.24839532905421 0.0 52 0.0 0.0 0.0015466707911221097 8.862423633129689 0.0 53 0.0 0.0 0.0015466707911221097 9.456345216920578 0.0 54 0.0 0.0 0.0015466707911221097 10.081200216533912 0.0 55 0.0 0.0 0.0015466707911221097 10.68285515428041 0.0 56 0.0 0.0 0.0015466707911221097 11.29688345835589 0.0 57 0.0 0.0 0.0015466707911221097 11.79645812388833 0.0 58 0.0 0.0 0.0015466707911221097 12.319232851287603 0.0 59 0.0 0.0 0.0015466707911221097 12.790967442579847 0.0 60 0.0 0.0 0.0015466707911221097 13.259608692289847 0.0 61 0.0 0.0 0.0015466707911221097 13.745263320702188 0.0 62 0.0 0.0 0.0015466707911221097 14.300518134715025 0.0 63 0.0 0.0 0.0015466707911221097 14.841852911607765 0.0 64 0.0 0.0 0.0015466707911221097 15.298120794988787 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGTC 25 2.8127924E-6 69.99149 68 ACGTCGC 20 7.876985E-5 69.99149 70 GGATCCT 15 0.0022164618 69.991486 26 GACGTCG 30 8.297331E-6 58.32624 69 TGTGACC 25 2.2772214E-4 56.473637 1 GTGACCA 25 2.3755658E-4 55.993195 2 GGACTAG 20 0.006695513 52.94403 1 GACAGAG 20 0.006925874 52.49362 7 TAAAGGT 20 0.006925874 52.49362 5 AGCTTTA 20 0.006925874 52.49362 24 CCAATAG 20 0.006925874 52.49362 20 TGAGTTT 20 0.006925874 52.49362 35 TTGAGTT 20 0.006925874 52.49362 34 CTACAGT 20 0.006925874 52.49362 48 TACGTGA 20 0.006925874 52.49362 36 ACCGCCT 30 5.8416015E-4 46.66099 61 GTAAGGA 30 5.8416015E-4 46.66099 9 ATAGTGT 40 4.5498935E-5 43.744682 4 ATCTCGG 50 1.6939368E-4 34.995747 41 TGTAAGG 40 0.0024040742 34.995747 8 >>END_MODULE