FastQCFastQC Report
Wed 25 May 2016
SRR1780440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64655
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT32174.975639935039826No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTT30084.652385739695306Illumina PCR Primer Index 1 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC9721.5033640089706906No Hit
CTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC4990.7717887247699327No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG3070.4748279328744877No Hit
TATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCT2580.3990410641095043Illumina PCR Primer Index 1 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1920.29696079189544505No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGT1510.23354728945943853No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG1490.23045394787719437No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC1480.22890727708607223No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC940.1453870543654783No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTC940.1453870543654783No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG870.13456035882762354No Hit
ATACACATCTCCGAGCCCACGAGACTGGTCACAATATCGTATGCCGTCTT790.12218699249864666No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAACCTCGTATGCCGT750.11600030933415822No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT150.002215393170.032
TGCCGCC150.002215393170.041
TTTACAC1900.058.9473722
CAGGTCT252.3741394E-455.9999966
ACTCACG252.3741394E-455.99999628
CTTTACA2100.053.3333321
TCGTTGT200.00692254552.50000434
TCCATGC200.00692254552.50000442
ACCATCG200.00692254552.50000430
TTTCGTC200.00692254552.50000458
CCAATCT200.00692254552.50000434
ACCAGCC200.00692254552.5000048
TGCTCGA200.00692254552.50000455
CGAGCCT200.00692254552.50000423
CTCGAAA200.00692254552.50000457
CGAGACC200.00692254552.50000423
TTGCCGC200.00692254552.50000440
AACTACA200.00692254552.50000446
TCGAAAA200.00692254552.50000458
CTATACA1050.049.9999961
Produced by FastQC (version 0.11.5)