FastQCFastQC Report
Wed 25 May 2016
SRR1780439_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780439_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502076
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTTT254.3985863E-544.0686121
CGAATAT301.3041635E-436.64698430
GCATCGT250.002341590835.2302612
GTACGTA401.8060668E-533.03166613
GACGAAT401.8184392E-532.9987323
TGTACGT502.3420616E-630.82647912
ACTCTAA300.00572615629.34976438
GTCTTCA300.00574572929.3292791
ATGACCA300.005756937429.31758728
TGTAACC300.005773782329.3000662
GAATGCG407.052554E-427.48249841
GCGAACT508.36237E-526.38582828
AGTGACC508.402142E-526.3674343
GAACGAA609.650947E-625.69642415
TGACGGT609.665911E-625.69129613
CTTTATA801.3210592E-724.7219312
TATACCG1001.7626007E-924.1725566
TTCGTTC551.577203E-424.03262319
TACGTAG551.5814816E-424.0230314
CATTGAA551.5976143E-423.98711826
Produced by FastQC (version 0.11.5)