FastQCFastQC Report
Wed 25 May 2016
SRR1780439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780439_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502076
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGT305916.092902269775891No Hit
ATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCTT237224.724782702220381RNA PCR Primer, Index 32 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC76681.5272588213736564No Hit
CTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC37210.741122857894024No Hit
TCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG36550.7279774376787578No Hit
TATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTCT20410.40651216150542946No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC19760.3935659143237279No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC13270.2643026155402768No Hit
CTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG12120.2413977166803432No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCC8490.16909790549637901No Hit
CTTATAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCG7030.14001864259594166No Hit
CTTAACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC6970.13882360439455382No Hit
TTATACACATCTCCGAGCCCACGAGACCAATCTTAATCTCGTATGCCGTC6150.12249141564225337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT24450.042.10814344
GCCGTCT27150.037.92059343
TTTACAC9400.036.2782362
CTTATAA1050.035.6348571
CTTTACA10600.032.807011
CTATACA5850.031.9800011
TTACACA10950.031.745733
TCTTTAT1950.030.4750581
CCGACGT300.00573779129.33758413
ACCATGG300.0057433829.3317431
TAAACCG300.0057433829.331747
CGACATA300.0057433829.3317444
CGCTATG300.0057433829.3317429
TTATACA44950.028.8778342
CTTATAC45750.028.5766121
TTAATAC2300.027.7403872
CTTTATA2150.027.6291582
AATCGGG407.0286007E-427.49850523
CGCCATT1250.026.39856517
TATACAC51350.025.921333
Produced by FastQC (version 0.11.5)