FastQCFastQC Report
Wed 25 May 2016
SRR1780438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64857
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTATT150.002216198769.9938366
GAAACCC307.99669E-658.69021
ATTTAGT200.006925055352.49537345
ACAAAGG200.006925055352.49537327
GCATTTC200.006925055352.49537356
CCATACG200.006925055352.49537333
CATACGG200.006925055352.49537334
TATGTTT200.006925055352.49537342
CAGAGTT200.006925055352.49537334
TGTAAGG200.006925055352.4953733
GAAAACC305.665068E-446.952161
GCAGAGT350.001247569339.99647533
TGACTAT350.001247569339.99647537
TAATAGG501.6936618E-434.9969142
TAGCTTT400.00240373534.99691443
ATATAGG450.0041528231.3014411
GGTATGG450.0041528231.3014411
CGACGAT450.00428068331.1083722
GAAACAC604.7598613E-429.34511
CATTTCA604.935776E-429.16409557
Produced by FastQC (version 0.11.5)