##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780438_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64857 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.172903464545076 34.0 23.0 34.0 12.0 34.0 2 29.19211496060564 34.0 24.0 34.0 12.0 34.0 3 29.410086806358603 34.0 27.0 34.0 11.0 34.0 4 29.71119539910881 34.0 31.0 34.0 11.0 34.0 5 29.565521069429668 34.0 31.0 34.0 11.0 34.0 6 32.02009035262192 37.0 31.0 38.0 11.0 38.0 7 31.265152566415345 36.0 27.0 38.0 11.0 38.0 8 30.837365280540265 36.0 26.0 38.0 11.0 38.0 9 31.318469864471066 37.0 27.0 38.0 12.0 38.0 10-11 31.472208088564074 37.0 27.0 38.0 11.0 38.0 12-13 31.817398276207655 37.0 29.0 38.0 11.0 38.0 14-15 31.456411798263872 37.0 27.0 38.0 11.0 38.0 16-17 31.268359621937492 37.0 27.0 38.0 11.0 38.0 18-19 31.276716468538474 37.0 26.5 38.0 11.0 38.0 20-21 31.388015171839584 37.0 27.0 38.0 11.0 38.0 22-23 31.226243890405044 37.0 27.0 38.0 11.0 38.0 24-25 31.633547650986017 37.0 27.0 38.0 11.0 38.0 26-27 31.611283284764944 37.0 27.0 38.0 11.0 38.0 28-29 31.63455756510477 37.0 27.0 38.0 11.0 38.0 30-31 31.480649737113957 37.0 27.0 38.0 11.0 38.0 32-33 31.356862019519866 37.0 26.5 38.0 11.0 38.0 34-35 31.288773763818863 37.0 27.0 38.0 11.0 38.0 36-37 31.23050711565444 37.0 26.5 38.0 11.0 38.0 38-39 31.583252386018472 37.0 27.0 38.0 11.0 38.0 40-41 31.232850733151395 37.0 26.5 38.0 11.0 38.0 42-43 31.523837056909816 37.0 27.0 38.0 11.0 38.0 44-45 30.718704226220762 37.0 26.0 38.0 11.0 38.0 46-47 31.335707787902617 37.0 27.0 38.0 11.0 38.0 48-49 31.207186579706125 37.0 26.5 38.0 11.0 38.0 50-51 31.118768984072652 37.0 26.5 38.0 11.0 38.0 52-53 31.15420078017793 37.0 26.5 38.0 11.0 38.0 54-55 30.926900720045637 37.0 26.0 38.0 11.0 38.0 56-57 30.865010715882633 36.5 26.0 38.0 11.0 38.0 58-59 30.791371787162525 36.5 25.5 38.0 11.0 38.0 60-61 30.846924772962055 37.0 26.0 38.0 11.0 38.0 62-63 30.839701188769137 37.0 26.0 38.0 11.0 38.0 64-65 30.939698105061908 37.0 26.0 38.0 11.0 38.0 66-67 30.91259231848528 37.0 26.0 38.0 11.0 38.0 68-69 30.44754613996947 36.0 24.5 38.0 11.0 38.0 70-71 30.274835407126446 36.0 24.5 38.0 11.0 38.0 72-73 30.287239619470526 36.0 24.5 38.0 11.0 38.0 74-75 29.92262207626008 36.0 24.0 38.0 11.0 38.0 76 29.107143407804863 34.0 23.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.8775383233401683 1101 2 -1.9874699484616265 1101 3 -0.6468858627186513 1101 4 -2.3959562171784974 1101 5 -1.977364056136679 1101 6 -3.2841523634987446 1101 7 -2.0961880705212614 1101 8 -1.1024790234842818 1101 9 -0.856482703331789 1101 10-11 -1.5401216559361899 1101 12-13 -2.179901387050073 1101 14-15 -0.9583868813385017 1101 16-17 -3.0333027736660405 1101 18-19 -0.9985078640421463 1101 20-21 -1.56139979828664 1101 22-23 -1.848572304479564 1101 24-25 -2.167553289362246 1101 26-27 -2.088219120836765 1101 28-29 -1.7148304774447674 1101 30-31 -1.396321144068942 1101 32-33 -0.6437320025944082 1101 34-35 -2.136594148363301 1101 36-37 -0.8113617490377969 1101 38-39 -1.272202818551655 1101 40-41 -0.8711004209884514 1101 42-43 -2.2701075687517154 1101 44-45 -1.1735252067332347 1101 46-47 -1.638152999241779 1101 48-49 -1.3151213689065386 1101 50-51 -2.0547815658952686 1101 52-53 -1.2634008618472343 1101 54-55 -1.5907012628604882 1101 56-57 -1.702109429894147 1101 58-59 -1.305955724063633 1101 60-61 -1.4337724299478474 1101 62-63 -1.9587893176106839 1101 64-65 -1.3637117101205298 1101 66-67 -1.2602906811116377 1101 68-69 -0.5272558399582614 1101 70-71 -0.6939176741143456 1101 72-73 -1.606657534060421 1101 74-75 -1.9353743496393108 1101 76 -2.0823039707238635 1102 1 -0.431299448806719 1102 2 -0.03204359187247974 1102 3 0.3874236405831013 1102 4 -0.01934123009839439 1102 5 -0.3825463702337224 1102 6 1.0771023020194832 1102 7 1.716616982593866 1102 8 1.2696857769750913 1102 9 1.273864698333444 1102 10-11 -0.3554747997777099 1102 12-13 -3.6847822368921612 1102 14-15 -0.4932347136669506 1102 16-17 -1.135692954544595 1102 18-19 -0.2607817658509326 1102 20-21 -0.07629356802537046 1102 22-23 -0.7584201368080166 1102 24-25 0.6956752492538953 1102 26-27 -5.099775250609948 1102 28-29 -0.29152441944878404 1102 30-31 0.49335729405910556 1102 32-33 -5.534487098775859 1102 34-35 -0.6734158537896562 1102 36-37 0.7021323652315132 1102 38-39 0.6660328977848344 1102 40-41 0.4516816634215388 1102 42-43 0.34674571864420756 1102 44-45 -4.498245275639345 1102 46-47 -0.029841199069512214 1102 48-49 0.24305405457896612 1102 50-51 -4.876235772039397 1102 52-53 -0.32577525174100685 1102 54-55 0.9453308933267088 1102 56-57 -1.3481401505372723 1102 58-59 1.1828829207827631 1102 60-61 0.7157394850679424 1102 62-63 -3.0176107359282263 1102 64-65 -0.012577630304278387 1102 66-67 0.4044738896606823 1102 68-69 -6.130686790288436 1102 70-71 -0.13951055381575017 1102 72-73 1.1206249810127922 1102 74-75 -4.183292810736063 1102 76 -0.8621632874048828 1103 1 0.018674273437401467 1103 2 -0.566565993659367 1103 3 -0.5658019539511265 1103 4 0.44961817076877253 1103 5 -0.3900026834645729 1103 6 -0.8423235290145428 1103 7 0.35694019899118956 1103 8 0.6038402901796651 1103 9 0.6724982550277012 1103 10-11 -0.34159685995376776 1103 12-13 -1.4596147161984305 1103 14-15 -0.14195038834833085 1103 16-17 -0.5568432633082239 1103 18-19 -1.1322178452097553 1103 20-21 -0.5011884570018488 1103 22-23 -0.9869380713764038 1103 24-25 -0.170587396916126 1103 26-27 -1.4426342704496555 1103 28-29 -0.2475256019477996 1103 30-31 0.040328782693975995 1103 32-33 -4.4915558151074855 1103 34-35 -0.6747297415836364 1103 36-37 0.057177694360404985 1103 38-39 0.007824383791930956 1103 40-41 0.3649322218238531 1103 42-43 0.5598254716333031 1103 44-45 -2.7188798834438437 1103 46-47 0.5507986642861553 1103 48-49 0.910919643857639 1103 50-51 -0.16740973078331933 1103 52-53 -0.2497996900539725 1103 54-55 0.6002382642372304 1103 56-57 0.7587610451006235 1103 58-59 0.20602376955427815 1103 60-61 0.9790754461768643 1103 62-63 0.1645105359151593 1103 64-65 0.5308792084816893 1103 66-67 0.05332095312146734 1103 68-69 -2.206268185637274 1103 70-71 0.12832547298756225 1103 72-73 0.36022884384290776 1103 74-75 -1.6159560612944688 1103 76 -0.018483087693940803 1104 1 -0.9525090109737953 1104 2 0.36541645749740326 1104 3 0.19072362759102646 1104 4 0.21981038741492398 1104 5 0.8216565659751893 1104 6 -0.9655441836242709 1104 7 0.660036496690271 1104 8 1.751269510897398 1104 9 0.2826993092207992 1104 10-11 0.13021767781096116 1104 12-13 -0.2334050769597198 1104 14-15 0.14446503948362732 1104 16-17 0.06508332192675681 1104 18-19 1.1278111924445078 1104 20-21 0.5287960772909877 1104 22-23 0.6704807336609946 1104 24-25 1.337240144667689 1104 26-27 0.9792165643829058 1104 28-29 0.45709517260006294 1104 30-31 -0.07290228759608297 1104 32-33 -0.3655576457422107 1104 34-35 0.358284808806161 1104 36-37 0.32883757505352307 1104 38-39 0.4431051337209162 1104 40-41 1.4137123247181478 1104 42-43 1.4551321029497757 1104 44-45 -2.301447822086015 1104 46-47 -0.0494917122564722 1104 48-49 0.7374512223066496 1104 50-51 0.6474910046268363 1104 52-53 0.4157149392424948 1104 54-55 -0.21005432169213023 1104 56-57 0.8702403873346292 1104 58-59 0.8914544421547284 1104 60-61 0.36576546921761377 1104 62-63 0.23227558341697474 1104 64-65 0.24534753534467413 1104 66-67 0.42729122108851314 1104 68-69 -2.7369944426205173 1104 70-71 0.3916054654125176 1104 72-73 1.3802553564837545 1104 74-75 0.09707616418923237 1104 76 -0.1578953908217997 1105 1 -1.4344877458809862 1105 2 -0.5358570452358222 1105 3 -1.975041975821 1105 4 -0.9622644609060949 1105 5 -0.4357471704337712 1105 6 -0.15014200905828545 1105 7 0.37115561725253343 1105 8 0.8955297379653402 1105 9 -0.4887009430769105 1105 10-11 0.2487074957961859 1105 12-13 -1.2111638322910139 1105 14-15 -0.7658341135169131 1105 16-17 0.19698396468521295 1105 18-19 0.2855423151693266 1105 20-21 -1.062196293706787 1105 22-23 -0.7017184613891629 1105 24-25 -1.0291622101109539 1105 26-27 -0.5767694991720909 1105 28-29 -1.5898802584085274 1105 30-31 -0.5105186749331381 1105 32-33 0.08366743985879666 1105 34-35 -0.0774543634170648 1105 36-37 0.3169735755340888 1105 38-39 -0.6017527986392714 1105 40-41 -0.10258190246993593 1105 42-43 0.12525284542590498 1105 44-45 0.442364876898786 1105 46-47 0.6366379207104309 1105 48-49 -0.0392352677516179 1105 50-51 -0.24185842770331334 1105 52-53 -0.6459177873749162 1105 54-55 -1.041915918376219 1105 56-57 -0.7530851367838345 1105 58-59 -0.5554579144260394 1105 60-61 0.3727787448828934 1105 62-63 -0.3081521212745635 1105 64-65 -0.02420553728102348 1105 66-67 -0.18870565522553662 1105 68-69 0.834054395007886 1105 70-71 0.8236052251289863 1105 72-73 -0.038258489854925415 1105 74-75 -0.6974213428690987 1105 76 -1.8345938950567984 1106 1 -0.11399438724721023 1106 2 0.5282163260481774 1106 3 -0.29793887651676343 1106 4 0.28182156059927976 1106 5 0.5064413178647733 1106 6 -0.5117485871734004 1106 7 0.7252353136472216 1106 8 0.95169672088754 1106 9 1.0972573111925392 1106 10-11 0.5034513288132629 1106 12-13 -0.5314990631575505 1106 14-15 -0.03424018562317599 1106 16-17 0.5291155269875567 1106 18-19 0.2933222013173342 1106 20-21 0.01183912691307043 1106 22-23 -0.036190314646592725 1106 24-25 0.17703639836469875 1106 26-27 0.8336036823585893 1106 28-29 -0.40455451520801944 1106 30-31 0.10908916820411463 1106 32-33 0.7088097548493124 1106 34-35 0.8624254594798337 1106 36-37 0.04720760462960172 1106 38-39 0.33015738478894363 1106 40-41 -0.16336621619542058 1106 42-43 0.7074160332816888 1106 44-45 1.0824660786698175 1106 46-47 0.5718687872505974 1106 48-49 0.4443194444558465 1106 50-51 0.09855902960218899 1106 52-53 0.022583161391690254 1106 54-55 0.05258123532397363 1106 56-57 0.9185151162205933 1106 58-59 -0.26851926799972503 1106 60-61 0.5701854495002294 1106 62-63 0.2706587579158217 1106 64-65 0.416970933307212 1106 66-67 1.1501305244076043 1106 68-69 1.1113788731862542 1106 70-71 -0.05517402850795605 1106 72-73 -0.017986510095280295 1106 74-75 -0.9554542334446836 1106 76 -0.4135312764609722 1107 1 -0.9650569064957466 1107 2 -1.216963840259183 1107 3 -0.40388282477669435 1107 4 -1.0893826278823937 1107 5 -0.42032184813460916 1107 6 -0.5354935825537517 1107 7 -0.026412361129494855 1107 8 0.31446235069967443 1107 9 -0.6909471852657312 1107 10-11 -0.7778082954552907 1107 12-13 -0.4573075017162225 1107 14-15 -0.5012250947700814 1107 16-17 0.3582039162699715 1107 18-19 -0.635796985624463 1107 20-21 -0.32280823988705265 1107 22-23 -1.2866984760263307 1107 24-25 -0.44646221574063105 1107 26-27 -1.0338431916789865 1107 28-29 -1.373756855830134 1107 30-31 -1.074218500413071 1107 32-33 -0.2775468820462841 1107 34-35 -0.7749013036861605 1107 36-37 -0.8203624600454837 1107 38-39 -0.5424019007053609 1107 40-41 -0.351709302120895 1107 42-43 -1.155780763761733 1107 44-45 -0.4621135332796733 1107 46-47 -0.9264989368023784 1107 48-49 -0.47314282841141875 1107 50-51 0.10259352298069047 1107 52-53 -0.1901670695907569 1107 54-55 -0.6945083858648182 1107 56-57 -0.601534160034408 1107 58-59 -0.3602346976270745 1107 60-61 -0.42132979534116544 1107 62-63 -0.7233972938242452 1107 64-65 -0.7113783121501349 1107 66-67 0.10873847020372551 1107 68-69 -0.004697505009001901 1107 70-71 -0.4795811735649309 1107 72-73 0.3214498927381051 1107 74-75 -0.2707760689851817 1107 76 -0.9801774113547204 1108 1 0.2524664512318253 1108 2 0.6248689097335856 1108 3 0.5769392515300353 1108 4 0.4039210081130946 1108 5 1.0894117436041313 1108 6 0.5603671099341696 1108 7 2.072456633114232 1108 8 1.1937831687263447 1108 9 0.6252716108350462 1108 10-11 -0.4056321940986649 1108 12-13 0.98228059235106 1108 14-15 1.0267607893524477 1108 16-17 -0.23557410577162585 1108 18-19 0.7771213563384549 1108 20-21 1.0387809533674108 1108 22-23 0.5260781286423253 1108 24-25 -0.287701983175765 1108 26-27 0.22599887247883288 1108 28-29 0.8091276427380336 1108 30-31 1.5567004779213747 1108 32-33 1.2687057574492435 1108 34-35 1.0728901978613798 1108 36-37 1.4011237567257986 1108 38-39 1.1671089609995313 1108 40-41 0.9310276739573204 1108 42-43 1.0295479799830218 1108 44-45 0.874715022446221 1108 46-47 0.45464421786482845 1108 48-49 0.7860484012319269 1108 50-51 1.6515652044478202 1108 52-53 0.5996712341898665 1108 54-55 0.7130297241117525 1108 56-57 1.6974881418949543 1108 58-59 -0.15373213767798077 1108 60-61 1.4262302983790036 1108 62-63 0.45323919340493646 1108 64-65 1.13739667266373 1108 66-67 0.6797372842386089 1108 68-69 1.6086489414345486 1108 70-71 1.911498054689968 1108 72-73 1.3749774158065726 1108 74-75 1.6976656885572652 1108 76 1.2085356718653202 1109 1 0.31652031863514196 1109 2 -0.2895532817949622 1109 3 0.3463625940714721 1109 4 -0.22234510606738667 1109 5 0.1472501413941849 1109 6 0.7179695500814987 1109 7 -2.0576078236076825 1109 8 -0.47207778891637986 1109 9 -0.27179906135648224 1109 10-11 0.3649709366564018 1109 12-13 -0.10447700433402218 1109 14-15 -0.4426654307212168 1109 16-17 -0.6678513847771512 1109 18-19 -0.5835773084865927 1109 20-21 0.16680236220718925 1109 22-23 0.7791283128043887 1109 24-25 0.28518590816862144 1109 26-27 0.5812446137311333 1109 28-29 -0.15011211324723917 1109 30-31 0.3931271584001905 1109 32-33 0.9962178430846045 1109 34-35 0.06653085163670269 1109 36-37 -0.4365546502723632 1109 38-39 0.2571181691026645 1109 40-41 -0.6390402358032645 1109 42-43 0.07778980779149336 1109 44-45 -0.3144395585850823 1109 46-47 -0.7663358696121527 1109 48-49 0.21882924837741768 1109 50-51 0.48756025509238654 1109 52-53 0.6392804653132593 1109 54-55 -0.1657398431073993 1109 56-57 0.3142871750441252 1109 58-59 0.7447773006277281 1109 60-61 -0.10227165834291085 1109 62-63 -0.9059343793390795 1109 64-65 0.3924611293856408 1109 66-67 -0.7315483971610206 1109 68-69 0.061960981029393736 1109 70-71 -0.6466568716452059 1109 72-73 -0.21718994146782933 1109 74-75 0.8462614528298289 1109 76 -1.9281758843041104 1110 1 0.7282051012438373 1110 2 0.40021121095866263 1110 3 0.018237594071472074 1110 4 0.0068215605992811845 1110 5 0.3469784022637512 1110 6 -0.04991994267212263 1110 7 -0.6572455047671006 1110 8 0.700929457460429 1110 9 -1.5351595686028574 1110 10-11 -0.8125652952276567 1110 12-13 0.006256691318153429 1110 14-15 0.2639695330469003 1110 16-17 0.18710785435328603 1110 18-19 0.9637505900641337 1110 20-21 0.5411230143811032 1110 22-23 0.40367541425366227 1110 24-25 0.7555210530961531 1110 26-27 -0.7440497703268356 1110 28-29 0.7399467635643546 1110 30-31 -0.3520041821795168 1110 32-33 1.3233238213454719 1110 34-35 1.0681159965642344 1110 36-37 -0.14284994012743724 1110 38-39 0.5442558502620862 1110 40-41 0.6492296917329661 1110 42-43 0.7767254961972938 1110 44-45 1.3994417819946214 1110 46-47 1.1019838767646597 1110 48-49 0.6626698280875587 1110 50-51 0.7879452188605001 1110 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2116 42-43 -0.03835602554183737 2116 44-45 1.4787896080815806 2116 46-47 0.2685599637211844 2116 48-49 0.6448709150440806 2116 50-51 0.13808108842571798 2116 52-53 1.085634631979925 2116 54-55 0.43530182355926783 2116 56-57 -0.3825878249558734 2116 58-59 -1.0510560327056062 2116 60-61 -0.6527924916762444 2116 62-63 1.2638572873275855 2116 64-65 0.7132944627189737 2116 66-67 0.35490993617231226 2116 68-69 0.6374818143627259 2116 70-71 -2.148740204978541 2116 72-73 -0.4255232748011615 2116 74-75 -0.9964468805035054 2116 76 -2.083384217637441 2117 1 -0.33701229006051037 2117 2 0.3489689749338183 2117 3 0.6991382151894854 2117 4 0.4874116227110825 2117 5 0.36095355754325453 2117 6 -0.7525596942249173 2117 7 -0.7737051320962927 2117 8 0.2739108239200583 2117 9 1.965616828912669 2117 10-11 -0.053636723799083796 2117 12-13 0.47248339939268647 2117 14-15 -0.28882549800900037 2117 16-17 0.8338469847880674 2117 18-19 0.731995931679041 2117 20-21 0.6265267410891759 2117 22-23 0.6905542962412383 2117 24-25 0.8871204319781469 2117 26-27 1.4543197948905622 2117 28-29 0.5815616703966526 2117 30-31 0.5940361904912876 2117 32-33 1.139317610165346 2117 34-35 0.7490166176822477 2117 36-37 -0.7410642258417219 2117 38-39 -0.01280936712921843 2117 40-41 -0.5223541591987129 2117 42-43 -0.08973413113190176 2117 44-45 0.7142709745412112 2117 46-47 0.6881640009882624 2117 48-49 0.27058908274594273 2117 50-51 1.24601975302199 2117 52-53 0.927113669247003 2117 54-55 -0.18958171681340374 2117 56-57 -0.13650863240929212 2117 58-59 0.14368387412669037 2117 60-61 0.8285157381374191 2117 62-63 0.19221520658224733 2117 64-65 0.23821682296742708 2117 66-67 -0.37230279674694344 2117 68-69 0.6322411311329112 2117 70-71 1.2338102608599684 2117 72-73 1.3746514146398319 2117 74-75 0.7967014114219566 2117 76 -0.020728938134336516 2118 1 -0.20811203430603342 2118 2 0.19292220840111796 2118 3 -0.0038212294579409445 2118 4 0.7347627370698682 2118 5 -0.3053233880175803 2118 6 1.1941337657677735 2118 7 -0.1571176275292494 2118 8 -3.6012444555830463 2118 9 -0.4380368064545195 2118 10-11 0.4299219170485564 2118 12-13 0.5449715250777416 2118 14-15 -1.3665931267996463 2118 16-17 0.05558611522285162 2118 18-19 -1.003736622212081 2118 20-21 -1.7469844024986934 2118 22-23 -0.897955020528947 2118 24-25 -0.15237536634118243 2118 26-27 0.07478015294682194 2118 28-29 -0.31926039756096003 2118 30-31 -1.0232637729723564 2118 32-33 -0.1147196569153941 2118 34-35 0.4918372241857192 2118 36-37 -0.09102768948804751 2118 38-39 -0.5080779093287084 2118 40-41 -0.9721897456071886 2118 42-43 -0.6514074961300764 2118 44-45 -0.31753392133018465 2118 46-47 -0.292837095102346 2118 48-49 -0.3850923202500347 2118 50-51 0.5456178531315992 2118 52-53 -0.9156521327259561 2118 54-55 -0.9856540587936742 2118 56-57 0.1479268809264802 2118 58-59 0.24030426141203876 2118 60-61 -1.4180498446174212 2118 62-63 0.01477640497464705 2118 64-65 -0.4742055372810263 2118 66-67 -1.7204577108865102 2118 68-69 0.6113788731862577 2118 70-71 -0.4110563814491286 2118 72-73 -1.4650453336246905 2118 74-75 -0.6083954099152713 2118 76 -0.2841195117550903 2119 1 0.9836763589035975 2119 2 1.8123063378918403 2119 3 0.20699598333321845 2119 4 0.7106806210019663 2119 5 1.1373927588438484 2119 6 0.706609091468529 2119 7 -1.801176056269874 2119 8 1.6678830611954787 2119 9 0.10092884692092241 2119 10-11 0.9948646726742965 2119 12-13 0.8968862506995379 2119 14-15 -0.07991724839459025 2119 16-17 -0.47998435457581223 2119 18-19 -0.9552565590458748 2119 20-21 -0.04240485255791171 2119 22-23 1.2077496774576275 2119 24-25 1.0601789171171632 2119 26-27 0.45227719203091254 2119 28-29 0.5794565388780413 2119 30-31 0.4850070521362113 2119 32-33 1.419508531004066 2119 34-35 0.5988644646120918 2119 36-37 -0.11919586951173855 2119 38-39 -0.5446156116579601 2119 40-41 0.8398118335479623 2119 42-43 0.008560920766889524 2119 44-45 -0.3738110630593603 2119 46-47 0.7375616415735315 2119 48-49 -0.2722222057612882 2119 50-51 1.8215752159424952 2119 52-53 -1.2986564754026269 2119 54-55 -1.593766968387044 2119 56-57 1.0885111683327153 2119 58-59 0.9195186317239212 2119 60-61 0.9351479445653652 2119 62-63 0.8838447034349706 2119 64-65 1.007673657349848 2119 66-67 1.0152706744273452 2119 68-69 0.04540966671171631 2119 70-71 -0.22590043987786856 2119 72-73 -1.6712237781568682 2119 74-75 -0.5982295986243145 2119 76 1.091364104510209 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 16.0 13 134.0 14 482.0 15 1050.0 16 1639.0 17 2105.0 18 2245.0 19 2089.0 20 1820.0 21 1460.0 22 1075.0 23 896.0 24 785.0 25 766.0 26 864.0 27 967.0 28 1056.0 29 1240.0 30 1539.0 31 2016.0 32 2483.0 33 3267.0 34 4519.0 35 6593.0 36 10962.0 37 12785.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.25415806065877 22.278819126302547 10.70302672650754 28.763996086531147 2 16.74915583514501 28.3269346408252 31.59874801486347 23.32516150916632 3 15.48483586968253 31.567910942535114 27.76724177806559 25.180011409716762 4 10.88548653190866 27.81503924017454 33.273201042293046 28.026273185623758 5 10.452225665695298 36.338406031731346 31.902493177297746 21.306875125275607 6 21.68000370044868 37.02144718380437 17.4553247914643 23.84322432428265 7 21.04321815686819 31.64037806250675 20.34784217586382 26.96856160476124 8 18.96171577470435 38.96572459410704 18.042771019319424 24.029788611869186 9 20.56061797492946 21.596743605162125 19.22537274311177 38.61726567679665 10-11 20.79205630892817 27.601705303246398 25.950367349456105 25.65587103836932 12-13 20.50742402516305 24.966464683842915 25.360408282837625 29.165703008156406 14-15 19.53605624681993 26.876821314584394 22.736173427694776 30.85094901090091 16-17 20.83738069907643 28.59598809689008 24.267234068797507 26.29939713523598 18-19 20.02174013599149 27.72098616957306 24.520868988698215 27.73640470573724 20-21 19.125152258044622 28.116471622184193 24.968006537459335 27.790369582311857 22-23 21.48495921797185 27.953806065652127 24.288820019427355 26.27241469694867 24-25 20.307753981836964 29.635197434355582 23.564148819711058 26.492899764096396 26-27 18.700371586721555 29.583545338205592 25.705783492915184 26.01029958215767 28-29 20.60070616895632 27.914488798433478 27.20600706168956 24.27879797092064 30-31 20.306983055028756 27.632329586629044 27.060301894938092 25.000385463404108 32-33 18.867662704102873 29.698413432628705 26.09587245786885 25.33805140539957 34-35 19.600814098709467 28.878147308694512 25.980233436637523 25.540805155958495 36-37 20.45500100220485 27.771096412106633 25.935519681761416 25.838382903927098 38-39 19.833633994788535 27.127372527252263 27.810413679325286 25.228579798633916 40-41 19.33021878902817 26.47131381346655 27.32010422930447 26.87836316820081 42-43 18.19695638096119 26.18607089442928 28.42484234546772 27.192130379141805 44-45 17.745193271350818 27.0957645281157 28.620657754752767 26.538384445780714 46-47 18.43440183788951 27.387945788426848 28.05788118476032 26.119771188923323 48-49 17.870854341088858 28.551274342013972 27.115037698320922 26.462833618576255 50-51 17.96567833849854 27.983872211172272 26.460520838151623 27.589928612177562 52-53 17.21633748091956 27.611514562807404 27.228363939127618 27.94378401714541 54-55 16.970411829100946 28.012396503075998 26.687173319765023 28.330018348058033 56-57 18.029665263579876 27.76107436359992 26.539155372588922 27.670105000231278 58-59 17.92250643723885 27.33398091185223 26.238493917387483 28.505018733521442 60-61 17.33428928257551 26.964706970720197 26.217678893565843 29.48332485313844 62-63 17.628783323311286 27.269223059962687 26.32406679309866 28.777926823627364 64-65 18.404335692369365 27.384091154385803 24.917125368117553 29.294447785127282 66-67 17.8484974636508 28.464159612686373 24.190141387976624 29.4972015356862 68-69 18.06589882356569 28.421758638234884 24.257982947099002 29.25435959110042 70-71 19.070416454661796 27.797307923585734 24.264150361564674 28.8681252601878 72-73 18.733521439474536 27.057989114513468 24.97186117150038 29.236628274511617 74-75 19.572289806805742 26.627041028724737 24.477697087438518 29.322972077031007 76 19.820528239048983 25.005781951061568 25.503800669164466 29.66988914072498 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 2.0 14 4.5 15 6.5 16 10.0 17 14.0 18 11.0 19 12.5 20 19.0 21 21.0 22 30.5 23 55.5 24 80.5 25 90.0 26 125.5 27 211.0 28 293.0 29 325.0 30 410.5 31 604.5 32 813.5 33 914.0 34 1028.5 35 1334.5 36 1697.5 37 1869.0 38 2085.5 39 2665.5 40 3318.5 41 3768.5 42 3929.0 43 4043.0 44 4318.0 45 4595.0 46 4711.0 47 4687.5 48 4507.5 49 4156.0 50 3961.0 51 3738.0 52 3258.5 53 2741.0 54 2480.0 55 2352.0 56 1953.5 57 1486.5 58 1290.0 59 1125.5 60 842.0 61 627.0 62 531.0 63 473.0 64 370.5 65 277.5 66 193.0 67 157.0 68 133.5 69 98.0 70 67.0 71 48.0 72 42.5 73 31.5 74 25.5 75 25.0 76 17.5 77 13.0 78 12.0 79 8.0 80 8.0 81 7.5 82 7.0 83 7.0 84 6.5 85 4.5 86 6.5 87 10.0 88 8.0 89 5.0 90 4.0 91 4.5 92 5.0 93 5.0 94 3.5 95 2.5 96 3.0 97 2.5 98 1.0 99 2.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.715420078017793 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.709268082088288E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 64857.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.07195830827821 #Duplication Level Percentage of deduplicated Percentage of total 1 81.67552122862628 56.41488197110567 2 8.862002767980712 12.242317714356199 3 3.9890173668467344 8.26587723761506 4 1.9777668645921693 5.4643292165841775 5 1.145140408053931 3.9548545261112906 6 0.7009241483994821 2.9048522133308663 7 0.5245769900441984 2.5363491990070464 8 0.31251395151569267 1.7268760503877763 9 0.22545649359346398 1.4015449373236506 >10 0.5870797803473369 5.088116934178269 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006167414465670629 0.0 2 0.0 0.0 0.0 0.006167414465670629 0.0 3 0.0 0.0 0.0 0.006167414465670629 0.0 4 0.0 0.0 0.0 0.007709268082088287 0.0 5 0.0 0.0 0.0 0.009251121698505943 0.0 6 0.0 0.0 0.0 0.009251121698505943 0.0 7 0.0 0.0 0.0 0.009251121698505943 0.0 8 0.0 0.0 0.0 0.010792975314923601 0.0 9 0.0 0.0 0.0 0.012334828931341258 0.0 10 0.0 0.0 0.0 0.018502243397011887 0.0 11 0.0 0.0 0.0 0.018502243397011887 0.0 12 0.0 0.0 0.0 0.018502243397011887 0.0 13 0.0 0.0 0.0 0.018502243397011887 0.0 14 0.0 0.0 0.0 0.020044097013429546 0.0 15 0.0 0.0 0.0 0.02312780424626486 0.0 16 0.0 0.0 0.0 0.024669657862682517 0.0 17 0.0 0.0 0.0 0.027753365095517832 0.0 18 0.0 0.0 0.0 0.032378925944770806 0.0 19 0.0 0.0 0.0 0.032378925944770806 0.0 20 0.0 0.0 0.0 0.03392077956118846 0.0 21 0.0 0.0 0.0 0.03854634041044143 0.0 22 0.0 0.0 0.0 0.04163004764327675 0.0 23 0.0 0.0 0.0 0.04625560849252972 0.0 24 0.0 0.0 0.0 0.049339315725365034 0.0 25 0.0 0.0 0.0 0.05088116934178269 0.0 26 0.0 0.0 0.0 0.05396487657461801 0.0 27 0.0 0.0 0.0 0.07555082720446521 0.0 28 0.0 0.0 0.0 0.0971367778343124 0.0 29 0.0 0.0 0.0 0.11101346038207133 0.0 30 0.0 0.0 0.0 0.13105755739550087 0.0 31 0.0 0.0 0.0 0.1773131658880306 0.0 32 0.0 0.0 0.0 0.21431765268205435 0.0 33 0.0 0.0 0.0 0.2451547250104075 0.0 34 0.0 0.0 0.0 0.3037451624342785 0.0 35 0.0 0.0 0.0 0.3854634041044143 0.0 36 0.0 0.0 0.0 0.5226883759655858 0.0 37 0.0 0.0 0.0 0.7940546124550936 0.0 38 0.0 0.0 0.0 1.1209275791356368 0.0 39 0.0 0.0 0.0 1.5264350802534807 0.0 40 0.0 0.0 0.0 1.9319425813713247 0.0 41 0.0 0.0 0.0 2.4777587615831753 0.0 42 0.0 0.0 0.0 3.0389934779592025 0.0 43 0.0 0.0 0.0 3.5693911220068766 0.0 44 0.0 0.0 0.0 4.102872473287386 0.0 45 0.0 0.0 0.0 4.718072066238031 0.0 46 0.0 0.0 0.0 5.229967466888693 0.0 47 0.0 0.0 0.0 5.789660329648303 0.0 48 0.0 0.0 0.0 6.329309095394483 0.0 49 0.0 0.0 0.0 6.867416007524246 0.0 50 0.0 0.0 0.0 7.385478822640579 0.0 51 0.0 0.0 0.0 7.9205020275375055 0.0 52 0.0 0.0 0.0 8.517199377091139 0.0 53 0.0 0.0 0.0 9.035262192207473 0.0 54 0.0 0.0 0.0 9.554866860940223 0.0 55 0.0 0.0 0.0 10.04980187181029 0.0 56 0.0 0.0 0.0 10.56323912607737 0.0 57 0.0 0.0 0.0 10.974914041660885 0.0 58 0.0 0.0 0.0 11.472932759763788 0.0 59 0.0 0.0 0.0 11.940114405538338 0.0 60 0.0 0.0 0.0 12.342538199423347 0.0 61 0.0 0.0 0.0 12.812803552430733 0.0 62 0.0 0.0 0.0 13.23527144332917 0.0 63 0.0 0.0 0.0 13.781087623541021 0.0 64 0.0 0.0 0.0 14.292983024191683 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTATT 15 0.0022161987 69.99383 66 GAAACCC 30 7.99669E-6 58.6902 1 ATTTAGT 20 0.0069250553 52.495373 45 ACAAAGG 20 0.0069250553 52.495373 27 GCATTTC 20 0.0069250553 52.495373 56 CCATACG 20 0.0069250553 52.495373 33 CATACGG 20 0.0069250553 52.495373 34 TATGTTT 20 0.0069250553 52.495373 42 CAGAGTT 20 0.0069250553 52.495373 34 TGTAAGG 20 0.0069250553 52.495373 3 GAAAACC 30 5.665068E-4 46.95216 1 GCAGAGT 35 0.0012475693 39.996475 33 TGACTAT 35 0.0012475693 39.996475 37 TAATAGG 50 1.6936618E-4 34.996914 2 TAGCTTT 40 0.002403735 34.996914 43 ATATAGG 45 0.00415282 31.301441 1 GGTATGG 45 0.00415282 31.301441 1 CGACGAT 45 0.004280683 31.10837 22 GAAACAC 60 4.7598613E-4 29.3451 1 CATTTCA 60 4.935776E-4 29.164095 57 >>END_MODULE