FastQCFastQC Report
Wed 25 May 2016
SRR1780438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780438_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64857
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT32715.043403179302157No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT30714.735032456018626RNA PCR Primer, Index 35 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC10551.6266555653206287No Hit
CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC4740.7308386141819696No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG3490.5381069121297625No Hit
TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT2790.4301771589805264No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1850.2852429190372666No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC1500.23127804246264858No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT1490.22973618884623093No Hit
CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG1190.1834805803537012No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC990.15264350802534807No Hit
CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG870.13414126462833617No Hit
CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC850.13105755739550087No Hit
TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC800.12334828931341259No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT800.12334828931341259No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT740.11409716761490664No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT720.11101346038207133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA650.059.2307741
TTAATAC252.3741944E-455.9999963
CCAGGGA200.006922642752.53
TTTCAGC200.006922642752.533
GCCGAGC200.006922642752.567
TCTTTAT352.0655536E-550.01
ATAACAC352.0655536E-550.04
CTTTATA352.0655536E-550.02
TTTACAC1800.048.6111152
TTACACA2000.047.2499963
CTTATAA404.546788E-543.751
CTTTACA2000.043.7499961
CTTAATA350.001247028740.02
TAATACA350.001247028740.04
TAACACA459.106207E-538.888895
TTTATAC556.60202E-638.181823
TCAACTA400.002402696335.05
CACAGAA552.9626352E-431.81818450
TATAACA552.9626352E-431.8181843
TCTTAAT500.00716109327.9999981