##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780438_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64857 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.84808116317437 34.0 34.0 34.0 31.0 34.0 2 33.32036634441926 34.0 34.0 34.0 33.0 34.0 3 33.51044605825123 34.0 34.0 34.0 34.0 34.0 4 33.501179518016556 34.0 34.0 34.0 34.0 34.0 5 33.53116857085588 34.0 34.0 34.0 34.0 34.0 6 37.26268560062908 38.0 37.0 38.0 36.0 38.0 7 36.884916046070586 38.0 37.0 38.0 35.0 38.0 8 36.954361132954034 38.0 37.0 38.0 35.0 38.0 9 36.74069105879088 38.0 37.0 38.0 34.0 38.0 10-11 36.47336447877638 38.0 37.0 38.0 34.0 38.0 12-13 36.33224632653376 38.0 37.0 38.0 34.0 38.0 14-15 36.515356862019516 38.0 37.0 38.0 34.0 38.0 16-17 36.623070755662454 38.0 37.0 38.0 34.0 38.0 18-19 36.66369859845506 38.0 37.0 38.0 34.0 38.0 20-21 36.41262315555761 38.0 37.0 38.0 34.0 38.0 22-23 36.4465439351188 38.0 37.0 38.0 34.0 38.0 24-25 35.64615230430023 38.0 36.0 38.0 31.5 38.0 26-27 35.866714464128776 38.0 36.5 38.0 31.5 38.0 28-29 35.763710933283996 38.0 36.5 38.0 31.0 38.0 30-31 35.41587646668825 38.0 36.0 38.0 29.0 38.0 32-33 35.90363414897389 38.0 37.0 38.0 32.5 38.0 34-35 35.74190912314785 38.0 36.5 38.0 31.5 38.0 36-37 35.849900550441745 38.0 37.0 38.0 32.0 38.0 38-39 35.29263610712799 38.0 36.0 38.0 29.0 38.0 40-41 35.154817521624494 37.0 35.5 38.0 29.0 38.0 42-43 35.767619532201614 38.0 36.5 38.0 32.5 38.0 44-45 35.96149220592997 38.0 37.0 38.0 33.0 38.0 46-47 36.17444531816149 38.0 37.0 38.0 34.0 38.0 48-49 35.93002297361888 38.0 36.5 38.0 32.5 38.0 50-51 36.02779962070401 38.0 37.0 38.0 33.0 38.0 52-53 35.90096674221749 38.0 37.0 38.0 32.0 38.0 54-55 36.119169866012925 38.0 37.0 38.0 34.0 38.0 56-57 36.10250242841944 38.0 37.0 38.0 34.0 38.0 58-59 36.117227130456236 38.0 37.0 38.0 34.0 38.0 60-61 35.88609556408714 38.0 37.0 38.0 32.5 38.0 62-63 36.25593999105725 38.0 37.0 38.0 34.0 38.0 64-65 36.217293430161746 38.0 37.0 38.0 34.0 38.0 66-67 35.36234330912623 38.0 36.0 38.0 30.5 38.0 68-69 33.99564426353362 37.5 34.0 38.0 23.0 38.0 70-71 33.16137810876236 37.0 33.0 38.0 20.5 38.0 72-73 32.255824352036015 37.0 29.5 38.0 11.0 38.0 74-75 32.580870222181105 37.0 32.0 38.0 11.0 38.0 76 32.1997933916154 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5282264046770067 1101 2 0.5784386384561415 1101 3 -0.20945056107017024 1101 4 0.2661150533096759 1101 5 0.24479044388372984 1101 6 -0.8824711930352294 1101 7 0.14591034869312125 1101 8 0.49754956625844216 1101 9 0.1408417277984526 1101 10-11 0.15564437399465447 1101 12-13 0.6558117335420235 1101 14-15 0.8403762232563068 1101 16-17 0.031199470168168375 1101 18-19 -0.5056372175643631 1101 20-21 0.20864155545511665 1101 22-23 0.6312699124816632 1101 24-25 -2.219356305422785 1101 26-27 -0.7825870697986161 1101 28-29 0.18436776155239443 1101 30-31 0.9802459338584342 1101 32-33 0.4321654275489095 1101 34-35 0.04678022857737574 1101 36-37 0.1677284938803183 1101 38-39 -0.13108420947273913 1101 40-41 1.1744291996178546 1101 42-43 0.5717912563422871 1101 44-45 -0.02240261690098322 1101 46-47 0.763227116763673 1101 48-49 -1.2006125878798315 1101 50-51 -1.8516239295534973 1101 52-53 -2.04352219082363 1101 54-55 -1.1810467795091952 1101 56-57 -1.2886060344283905 1101 58-59 -1.643954528929882 1101 60-61 -2.2921971210484173 1101 62-63 -0.627146872468721 1101 64-65 -0.6614647984395177 1101 66-67 -2.05085722971684 1101 68-69 -0.8696091803170205 1101 70-71 -1.4729819021332915 1101 72-73 -1.9486846849846735 1101 74-75 -1.3230407398802342 1101 76 -1.3818953385303807 1102 1 -1.101259670545499 1102 2 0.2069916100323752 1102 3 0.28638636112334126 1102 4 0.3360978267808221 1102 5 -0.045691898924197005 1102 6 0.0756109200856443 1102 7 0.11576392319786066 1102 8 0.2921949868728504 1102 9 0.3886166344880877 1102 10-11 0.5945260851631886 1102 12-13 0.4953537949833162 1102 14-15 -0.6684523727815872 1102 16-17 0.09938781355030812 1102 18-19 -0.33965243433151926 1102 20-21 0.9208867463824788 1102 22-23 0.6525877419390298 1102 24-25 -4.609321852365074 1102 26-27 -0.8601423382453603 1102 28-29 0.2482135841191493 1102 30-31 1.7998698213117805 1102 32-33 0.8079190881862885 1102 34-35 0.7799125857407319 1102 36-37 -2.520650776863299 1102 38-39 -0.1995596616691202 1102 40-41 1.0944693948518491 1102 42-43 0.7674559132472538 1102 44-45 0.7778414255911201 1102 46-47 0.9831524684719639 1102 48-49 -3.7847713590541048 1102 50-51 -0.7990471279456983 1102 52-53 0.14744105924814477 1102 54-55 0.41642666292548824 1102 56-57 0.8784912954596393 1102 58-59 0.11340263443503318 1102 60-61 -5.309818424522437 1102 62-63 -2.6236297894942737 1102 64-65 -0.04446795663647407 1102 66-67 1.2591126238576607 1102 68-69 -0.8726238228665437 1102 70-71 -1.183791548989447 1102 72-73 -5.11420291639438 1102 74-75 -1.8853074639686618 1102 76 -0.6513469606951787 1103 1 0.8055856848611498 1103 2 0.6782503145390066 1103 3 0.18561116732488614 1103 4 0.3794232767873922 1103 5 0.2733529046495775 1103 6 0.3389140339997141 1103 7 0.709312734129405 1103 8 0.7095185974365066 1103 9 0.42855882342514917 1103 10-11 0.4789860772798562 1103 12-13 -0.6063246966734965 1103 14-15 -0.22898975288932633 1103 16-17 0.1404796543071143 1103 18-19 0.854950771554698 1103 20-21 -0.34921573686544605 1103 22-23 0.4434168051370406 1103 24-25 -3.084390831474259 1103 26-27 -0.2572156531842609 1103 28-29 0.10935206789263674 1103 30-31 0.9715621087904296 1103 32-33 1.057557769042944 1103 34-35 0.6408375627476985 1103 36-37 -0.4966887482205493 1103 38-39 0.1518724760263197 1103 40-41 0.3022279022753196 1103 42-43 -0.4283133680561235 1103 44-45 0.9900999987089776 1103 46-47 1.1247567254684228 1103 48-49 -2.561985259986969 1103 50-51 -0.4247040718426831 1103 52-53 -0.043417566425219434 1103 54-55 -0.39913633799423565 1103 56-57 0.6569369661993605 1103 58-59 -0.17842826820587732 1103 60-61 -3.9788993600105442 1103 62-63 -1.0810598249692447 1103 64-65 0.3254571517592595 1103 66-67 -0.3532740533201064 1103 68-69 0.005940097774633557 1103 70-71 0.14205262391818252 1103 72-73 -4.1786491126892145 1103 74-75 -2.168039693636249 1103 76 -2.624937816036134 1104 1 -0.044744145016075265 1104 2 0.2091028221929534 1104 3 0.30106740568615464 1104 4 -0.3178907401933273 1104 5 -0.657651103809215 1104 6 0.31609422526948094 1104 7 0.5016935061522574 1104 8 0.6296966108822062 1104 9 0.655084089340626 1104 10-11 -0.4676572330127229 1104 12-13 0.36911954787404966 1104 14-15 0.49131766749384553 1104 16-17 0.4568322724303968 1104 18-19 0.7850552439847078 1104 20-21 -0.14241713824789315 1104 22-23 0.1764281992600658 1104 24-25 0.59669997432065 1104 26-27 1.1024567263252578 1104 28-29 1.2906164683597652 1104 30-31 0.9152069656537307 1104 32-33 0.8602121893945593 1104 34-35 -0.02546613324068403 1104 36-37 1.155537997984041 1104 38-39 1.3551044931964071 1104 40-41 1.7088970939554002 1104 42-43 0.5243741930965413 1104 44-45 0.5750351374268945 1104 46-47 1.0411945627944377 1104 48-49 0.05995710658185516 1104 50-51 0.9409652145253986 1104 52-53 0.3781965483997638 1104 54-55 -0.11316278750974362 1104 56-57 0.4013898273551817 1104 58-59 -0.6459029391650688 1104 60-61 0.10695109607000575 1104 62-63 -0.06581505648141217 1104 64-65 0.5911043442599748 1104 66-67 0.3454287210383882 1104 68-69 2.2575892471607446 1104 70-71 1.4935502724994407 1104 72-73 0.050211790634335784 1104 74-75 -0.5811792321900491 1104 76 -0.7030554185358966 1105 1 0.9806358033229685 1105 2 0.26481856677155946 1105 3 0.27163267270123015 1105 4 0.061216606475746005 1105 5 -0.32569940079966386 1105 6 0.12449814189109532 1105 7 0.7886821527552499 1105 8 -0.3754094142274198 1105 9 0.6810647465160926 1105 10-11 0.5823667953407323 1105 12-13 -0.24492502472160993 1105 14-15 0.40250286602811514 1105 16-17 -0.054025539788021604 1105 18-19 0.6181182583416458 1105 20-21 0.4442050759648737 1105 22-23 0.23073227807307006 1105 24-25 0.51302123340637 1105 26-27 0.6700214527023789 1105 28-29 1.0421933290553866 1105 30-31 1.5487445399914535 1105 32-33 1.244590756103264 1105 34-35 -0.43957978735254244 1105 36-37 0.4259921338643835 1105 38-39 -0.1292920947773979 1105 40-41 0.5243893748468551 1105 42-43 0.6974384088711574 1105 44-45 1.1428701827804133 1105 46-47 0.7911044564689718 1105 48-49 -0.26373484992681995 1105 50-51 0.8940949075631863 1105 52-53 0.6050242050345958 1105 54-55 0.8137760002598284 1105 56-57 0.6860056740542859 1105 58-59 0.7191478207316067 1105 60-61 1.0514906527468781 1105 62-63 -0.5123519700394112 1105 64-65 0.8401281924007833 1105 66-67 0.602667262517329 1105 68-69 0.17101208611070007 1105 70-71 0.3000169031235842 1105 72-73 -0.40796385662943635 1105 74-75 -0.5499423726958454 1105 76 -0.331517003205807 1106 1 -0.5459929126795657 1106 2 0.36602033780255283 1106 3 0.18365038301116243 1106 4 0.2345246393663203 1106 5 -0.2985646759419822 1106 6 0.12007056440848629 1106 7 -1.4549611110018716 1106 8 0.36545080082633774 1106 9 -1.0797828183162892 1106 10-11 0.34852745315224354 1106 12-13 1.2018175432733216 1106 14-15 -0.7350050403602424 1106 16-17 0.07462721163513208 1106 18-19 0.6551834069817488 1106 20-21 0.29455295705280093 1106 22-23 0.48028952032480277 1106 24-25 -0.5120578304772536 1106 26-27 1.0275799599762578 1106 28-29 0.6266335567593728 1106 30-31 0.4983650333500833 1106 32-33 -0.44310690360577354 1106 34-35 1.2419508894343068 1106 36-37 1.0099579782666552 1106 38-39 -0.31768551666228007 1106 40-41 1.0050029105837268 1106 42-43 1.2048704139312534 1106 44-45 1.3123831492573288 1106 46-47 1.0312372837934447 1106 48-49 1.210680077334402 1106 50-51 -0.15612647131094093 1106 52-53 0.5263398280579992 1106 54-55 -0.06332709904442169 1106 56-57 0.5178209808221581 1106 58-59 0.6561180927113668 1106 60-61 0.9635399886102576 1106 62-63 1.118641072338832 1106 64-65 -1.2079426488389444 1106 66-67 0.3806242517646439 1106 68-69 1.4255977913605378 1106 70-71 0.39226864252901805 1106 72-73 0.7573429112390002 1106 74-75 -0.6969695706718113 1106 76 0.2894191131762298 1107 1 0.5937360679179093 1107 2 -0.7554764284432167 1107 3 0.09486073102471693 1107 4 0.050970589474111705 1107 5 -0.1536000932502617 1107 6 0.22062492227728825 1107 7 0.5881248144677969 1107 8 -0.22774291645114886 1107 9 -0.7398382541031694 1107 10-11 0.3133103296536319 1107 12-13 -0.03966690390868166 1107 14-15 0.1822416488913703 1107 16-17 0.8624159396917932 1107 18-19 0.7943272320510388 1107 20-21 0.6461698341701378 1107 22-23 -0.14578069833857654 1107 24-25 0.2868488526148383 1107 26-27 -0.4343387342029885 1107 28-29 0.7042436583916469 1107 30-31 0.9343213006737656 1107 32-33 0.9304687046479927 1107 34-35 -0.018212338160395802 1107 36-37 1.0673956130064255 1107 38-39 0.6369495310740945 1107 40-41 0.621895426752495 1107 42-43 1.2429825174113844 1107 44-45 0.4111666417119011 1107 46-47 0.8671473546280666 1107 48-49 0.9290816788126861 1107 50-51 0.913953602603435 1107 52-53 0.722459322976384 1107 54-55 0.5947110900532167 1107 56-57 0.8103371495933303 1107 58-59 0.8124316128838345 1107 60-61 0.9429363544864486 1107 62-63 0.7807291536446499 1107 64-65 1.1126609852848688 1107 66-67 -0.1335118738898089 1107 68-69 0.3007902767316537 1107 70-71 -0.06513819455135916 1107 72-73 -0.5620904118284464 1107 74-75 -1.1453372947247829 1107 76 -0.11122763767070865 1108 1 -0.31274624384628424 1108 2 0.31385914183245234 1108 3 0.10457985977424045 1108 4 0.27092372938906806 1108 5 0.06727867797417275 1108 6 -0.5404754219418493 1108 7 -0.0052048549082712725 1108 8 -0.3986375969637166 1108 9 -0.12339672796854728 1108 10-11 0.4353490326127627 1108 12-13 1.1158246930945808 1108 14-15 0.2260934321779402 1108 16-17 -0.6951322943772169 1108 18-19 0.3106270208914026 1108 20-21 0.8111925410631926 1108 22-23 -0.16753303411133658 1108 24-25 0.9395346297112468 1108 26-27 -0.5844934881875403 1108 28-29 -1.0569868527303896 1108 30-31 0.5728044223653583 1108 32-33 0.9047968987991624 1108 34-35 0.4058280425054761 1108 36-37 1.1248426061509633 1108 38-39 1.3439004436887103 1108 40-41 0.9574078504602355 1108 42-43 0.014018935218210515 1108 44-45 0.9011871791565795 1108 46-47 0.8876558733287041 1108 48-49 1.054895221955789 1108 50-51 0.2543545166643497 1108 52-53 0.09436062753800911 1108 54-55 -0.22853671880391602 1108 56-57 0.1570150009716471 1108 58-59 0.2533069129908654 1108 60-61 1.3400755752205953 1108 62-63 0.5789270651890135 1108 64-65 0.6657980076446464 1108 66-67 0.6281380639193372 1108 68-69 1.732958857333891 1108 70-71 0.14390985149879754 1108 72-73 0.6276061546579186 1108 74-75 0.20265668867170206 1108 76 1.0658572022240023 1109 1 -2.184350755816819 1109 2 0.10621641623392009 1109 3 0.20123616732488614 1109 4 0.12086042368004257 1109 5 -0.18171418574590348 1109 6 -0.8024171806895524 1109 7 -0.7267748364920621 1109 8 0.04461746749300488 1109 9 0.5046534561935161 1109 10-11 0.27263897276008464 1109 12-13 0.46156019033215046 1109 14-15 0.42862853807113055 1109 16-17 0.701434809674339 1109 18-19 0.682634387373902 1109 20-21 0.6175921727390659 1109 22-23 0.1833532458149989 1109 24-25 -0.6215553794968613 1109 26-27 -0.6863293537492368 1109 28-29 -1.8081090902994532 1109 30-31 -0.3586815352773627 1109 32-33 0.5852325571785428 1109 34-35 -0.9881348948794226 1109 36-37 0.7031258704235199 1109 38-39 1.1874921794161537 1109 40-41 -0.048275275690777164 1109 42-43 0.5169108551077173 1109 44-45 0.8105449139632128 1109 46-47 0.8360473328130524 1109 48-49 0.07321071458930817 1109 50-51 -0.21813627523251 1109 52-53 -0.7047876229223888 1109 54-55 -0.8331494029659865 1109 56-57 -0.19630279368765002 1109 58-59 -0.543452985720009 1109 60-61 0.4781539591984938 1109 62-63 -0.16196554530822738 1109 64-65 0.4732767139061522 1109 66-67 0.13635341843131243 1109 68-69 -0.6667735321688681 1109 70-71 -0.3311994947258796 1109 72-73 -0.4299732652315882 1109 74-75 -0.11538256086788579 1109 76 -0.5175049064316148 1110 1 -1.15219495871537 1110 2 -0.2561024243457908 1110 3 -0.05714245586352007 1110 4 -0.06278993863879379 1110 5 0.2691463215004788 1110 6 -0.0947631951823027 1110 7 0.3665222649572115 1110 8 0.5056681921306918 1110 9 0.805830992425399 1110 10-11 -0.4140684547761424 1110 12-13 0.4296308425060573 1110 14-15 0.8934156757522871 1110 16-17 0.8399085415584011 1110 18-19 -0.08548155465508245 1110 20-21 0.7299110133187838 1110 22-23 -0.6884764643299306 1110 24-25 -1.6131767563084551 1110 26-27 0.8899840520478719 1110 28-29 0.36784199665707007 1110 30-31 -0.27874494107447134 1110 32-33 0.7592588252944807 1110 34-35 1.0501350326568115 1110 36-37 1.3667354718728006 1110 38-39 1.0286380127494823 1110 40-41 -0.9557707467052765 1110 42-43 0.030158137716412625 1110 44-45 0.9260340443979942 1110 46-47 1.0073562096246533 1110 48-49 1.3980522000965507 1110 50-51 -0.8386072897252674 1110 52-53 0.8051761451935491 1110 54-55 -0.10613400441526011 1110 56-57 0.5653593439935136 1110 58-59 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46-47 -0.9228068338536062 2116 48-49 0.3174815479226396 2116 50-51 -1.3441779418991828 2116 52-53 0.34104571041094545 2116 54-55 -0.5326285696326494 2116 56-57 -0.6770319603543129 2116 58-59 -2.5986613190533348 2116 60-61 -2.2202835408015034 2116 62-63 0.22084695469177262 2116 64-65 -2.817869119427179 2116 66-67 -1.3475007482353618 2116 68-69 -1.7370860321688681 2116 70-71 1.2510921719407904 2116 72-73 0.6950267347684118 2116 74-75 0.5590966057987785 2116 76 -0.11646323976494699 2117 1 0.9215628052597893 2117 2 0.6025932278281232 2117 3 0.45683269941598326 2117 4 0.3207504340320142 2117 5 0.13172396125202823 2117 6 0.399025624693472 2117 7 0.6491309606093836 2117 8 0.5064445896462146 2117 9 -0.41233670943795886 2117 10-11 -0.1974535479438515 2117 12-13 -0.7605865487982868 2117 14-15 0.23697157637339927 2117 16-17 -0.6950293466403537 2117 18-19 -1.1755436664563206 2117 20-21 0.177892379778406 2117 22-23 0.24979993318560645 2117 24-25 -1.9217947687308126 2117 26-27 -1.3910718485732545 2117 28-29 -0.3165524132808173 2117 30-31 0.17467120799386038 2117 32-33 -1.2058032865067645 2117 34-35 0.39019714445805675 2117 36-37 -1.2264322299905501 2117 38-39 0.651697018960661 2117 40-41 0.6199280110586969 2117 42-43 0.6819438520021279 2117 44-45 0.3371365288700474 2117 46-47 -0.4643052810585786 2117 48-49 0.1049621379847494 2117 50-51 0.034063517728142756 2117 52-53 0.5202382569947943 2117 54-55 -0.0389756193220947 2117 56-57 0.026151269459354864 2117 58-59 -0.8683429960719735 2117 60-61 -1.2486996898698308 2117 62-63 -0.6762803745007702 2117 64-65 -1.1366967591787258 2117 66-67 -0.24484158674466983 2117 68-69 0.5720172287006946 2117 70-71 -0.26070907029524193 2117 72-73 1.3789358962590939 2117 74-75 1.0314374443080965 2117 76 0.8027914186201457 2118 1 0.012830616732202316 2118 2 0.1424909260378442 2118 3 -0.7634084405182477 2118 4 0.07570110995455792 2118 5 0.24261179464625826 2118 6 0.22595291734966594 2118 7 0.7568035948804877 2118 8 0.5713331537675188 2118 9 -1.426841641845705 2118 10-11 -1.148531370377171 2118 12-13 -1.1158295155502032 2118 14-15 0.5620537831691692 2118 16-17 -2.6341963177766345 2118 18-19 -0.8065812989006034 2118 20-21 -0.6730941017707295 2118 22-23 0.40381893208951425 2118 24-25 0.5967656989345116 2118 26-27 -2.913596510611974 2118 28-29 -0.7821899726523966 2118 30-31 -1.1428114372381515 2118 32-33 -0.3048716094881314 2118 34-35 -0.36687263997745845 2118 36-37 -3.428277315850984 2118 38-39 -0.44415610489757285 2118 40-41 -0.9920559129456805 2118 42-43 0.4460468845194825 2118 44-45 -2.8611462625073756 2118 46-47 -0.3569980103241974 2118 48-49 0.38420948909910635 2118 50-51 -1.0708323536638886 2118 52-53 -2.061895466059646 2118 54-55 -0.8368565108091204 2118 56-57 0.42664451023392047 2118 58-59 -0.9144701425827506 2118 60-61 -0.7070482466838541 2118 62-63 -0.8254765747199926 2118 64-65 -0.037354413544825604 2118 66-67 0.20121248705876127 2118 68-69 -1.318519855698277 2118 70-71 -0.2047901810003907 2118 72-73 1.339695852415467 2118 74-75 0.22655984109289307 2118 76 0.6600073484703444 2119 1 0.852072622259243 2119 2 -0.880920049090463 2119 3 0.25719953421526753 2119 4 -0.34897318929535004 2119 5 -0.16224410744612783 2119 6 -0.8141971023897767 2119 7 -0.9195880355972079 2119 8 0.2533702639135882 2119 9 -0.03904061539485326 2119 10-11 -0.6566640194099307 2119 12-13 -0.7101050781242293 2119 14-15 -0.07523401785729078 2119 16-17 -0.3412630370818093 2119 18-19 -0.15405465065740742 2119 20-21 -1.8675867981781522 2119 22-23 -0.15340570273085774 2119 24-25 1.041021522069137 2119 26-27 0.7539321115751534 2119 28-29 -0.10372570103770329 2119 30-31 1.0972398853042833 2119 32-33 0.6249941623239508 2119 34-35 0.45464334896846026 2119 36-37 0.07391376192240529 2119 38-39 -1.1286606449686403 2119 40-41 -0.6213492410151673 2119 42-43 0.14259248262449375 2119 44-45 0.5917949139632128 2119 46-47 0.23151363594504915 2119 48-49 -0.9619018413391132 2119 50-51 1.2104151789061888 2119 52-53 0.5970859788673195 2119 54-55 0.20388569211231555 2119 56-57 -1.0249346449180692 2119 58-59 -0.0576797754291789 2119 60-61 0.8423003518159433 2119 62-63 0.15247447147029192 2119 64-65 0.05893457184799189 2119 66-67 0.22760411753645826 2119 68-69 0.51511614903918 2119 70-71 0.22021968871930397 2119 72-73 1.0493681777214263 2119 74-75 0.5690588541209252 2119 76 0.9036709884229737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 4.0 22 9.0 23 12.0 24 47.0 25 64.0 26 186.0 27 320.0 28 526.0 29 953.0 30 1497.0 31 2151.0 32 3180.0 33 4681.0 34 6891.0 35 9130.0 36 12714.0 37 22490.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.75051266632746 23.01524893226637 11.486809442311547 32.74742895909462 2 15.902678199731717 18.593212760380528 44.73534082674191 20.768768213145844 3 15.363029433985536 26.820543657585148 36.44016837041491 21.3762585380144 4 10.836147216183296 23.718334181352823 35.87584994680605 29.569668655657832 5 9.99737884885209 39.58554974790693 37.6351049231386 12.78196648010238 6 21.077138936429378 39.924757543518815 17.486161863792653 21.511941656259154 7 21.024715913471177 35.98686340718812 20.329339932466812 22.65908074687389 8 18.909292751746147 36.65911158394622 23.516351357602108 20.91524430670552 9 18.676472855667082 18.974050603635693 18.924711287910327 43.4247652527869 10-11 19.557642197449773 25.709638126956225 30.64896618715019 24.083753488443808 12-13 22.38463080315155 19.62702561018857 27.869004116749156 30.119339469910727 14-15 22.649058698367178 23.284302388331252 20.92064079436298 33.145998118938586 16-17 24.41139738193256 27.38023652034476 20.66546402084586 27.542902076876825 18-19 22.33297870700156 26.468230106233715 21.40478282991813 29.794008356846604 20-21 21.223615029989055 24.183973973510955 21.382425952480073 33.209985044019916 22-23 23.094654393511878 29.726937724532434 21.629893457915106 25.54851442404058 24-25 23.914920517446074 31.554034259987358 21.733968577023298 22.79707664554327 26-27 25.33728047859136 28.646098339423652 25.312610820728683 20.7040103612563 28-29 26.94235009328214 23.101592734785758 28.01702206392525 21.939035108006845 30-31 29.299844272784743 21.59520175154571 30.02451547250104 19.08043850316851 32-33 23.58881847757374 22.229674514701575 32.779807885039396 21.40169912268529 34-35 18.31722096304177 25.15379989823766 36.67221733968577 19.8567617990348 36-37 18.028894336771668 26.983980140925425 28.758653653422144 26.22847186888077 38-39 20.34244568820636 24.00280617358188 28.382441371016238 27.27230676719552 40-41 19.253897035015495 24.66657415544968 30.948856715543428 25.130672093991397 42-43 24.730561080531015 23.03452210247159 32.16537921889696 20.069537598100435 44-45 19.433522981328153 25.240143700757052 29.639052068396627 25.687281249518172 46-47 24.648071912052668 21.68000370044868 27.619994757697704 26.051929629800945 48-49 22.306767195522458 21.03319610836147 28.834975407434822 27.82506128868125 50-51 17.63186703054412 21.824167013583732 32.63333179147972 27.910634164392434 52-53 18.937046116841667 21.487271998396473 37.160984936090166 22.414696948671693 54-55 17.570963812695624 21.540465948162883 33.76505234593028 27.12351789321122 56-57 23.011394298225326 21.649166628120327 29.947422791680157 25.39201628197419 58-59 18.274049061782076 28.039378941363307 32.386635212852894 21.299936784001726 60-61 19.886827944554945 35.281465377677044 24.594107035478054 20.23759964228996 62-63 15.766224154678754 41.12123594985892 22.525710409053765 20.58682948640856 64-65 15.609726012612363 42.14271397073562 21.353901660576344 20.893658356075672 66-67 15.72999059469294 41.268482970226806 21.518879997533034 21.48264643754722 68-69 15.679880352159367 39.268698829733104 21.817228672309852 23.234192145797678 70-71 16.40223877145104 37.41153614875803 22.002251106279967 24.183973973510955 72-73 16.98505943845691 34.029480241145905 22.81095332809103 26.174506992306153 74-75 17.357417086821776 33.35723206438781 23.150932050511123 26.13441879827929 76 17.416778451053858 33.40117489245571 23.348289313412586 25.833757343077846 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 1.0 13 1.0 14 0.5 15 0.5 16 2.5 17 4.0 18 4.0 19 6.5 20 16.0 21 23.0 22 24.5 23 33.5 24 50.5 25 60.0 26 91.5 27 166.0 28 267.5 29 326.0 30 364.5 31 477.5 32 678.5 33 805.0 34 915.0 35 1175.5 36 1426.0 37 1526.0 38 1784.5 39 2605.0 40 4170.5 41 6014.5 42 6855.0 43 6830.5 44 6399.5 45 5577.0 46 5161.0 47 4875.0 48 4154.5 49 3481.0 50 3242.0 51 2978.5 52 2401.0 53 1906.5 54 1726.0 55 1595.5 56 1249.0 57 915.5 58 798.0 59 643.0 60 462.5 61 365.5 62 294.0 63 235.0 64 166.0 65 127.0 66 86.5 67 75.0 68 56.5 69 34.5 70 21.5 71 12.0 72 9.0 73 3.0 74 1.5 75 3.0 76 1.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 64857.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.51132491481258 #Duplication Level Percentage of deduplicated Percentage of total 1 62.26274932214092 25.84609217200919 2 17.063477324220926 14.166551027645436 3 7.996879991085688 9.958832508441647 4 4.163726182074806 6.913671616016775 5 2.566578761653605 5.327104244723007 6 1.664004754299298 4.144502520930663 7 1.1440032685807673 3.324236396996469 8 0.6611447461278461 2.195599549778744 9 0.5831445232700665 2.17863915999815 >10 1.8200052000148574 10.748261560047489 >50 0.037142963265609334 1.1625576267789137 >100 0.02600007428592653 2.6288604159921056 >500 0.0 0.0 >1k 0.011142888979682799 11.405091200641412 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT 3271 5.043403179302157 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT 3071 4.735032456018626 RNA PCR Primer, Index 35 (95% over 21bp) CTTTACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 1055 1.6266555653206287 No Hit CTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 474 0.7308386141819696 No Hit TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 349 0.5381069121297625 No Hit TATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCT 279 0.4301771589805264 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 185 0.2852429190372666 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 150 0.23127804246264858 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGT 149 0.22973618884623093 No Hit CTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 119 0.1834805803537012 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC 99 0.15264350802534807 No Hit CTTATAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG 87 0.13414126462833617 No Hit CTTAACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 85 0.13105755739550087 No Hit TTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTC 80 0.12334828931341259 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCATATCGTATGCCGTCTT 80 0.12334828931341259 No Hit ATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGTCTT 74 0.11409716761490664 No Hit CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCAACTCGTATGCCGT 72 0.11101346038207133 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010792975314923601 0.0 2 0.0 0.0 0.0 0.010792975314923601 0.0 3 0.0 0.0 0.0 0.010792975314923601 0.0 4 0.0 0.0 0.0 0.012334828931341258 0.0 5 0.0 0.0 0.0 0.013876682547758916 0.0 6 0.0 0.0 0.0 0.013876682547758916 0.0 7 0.0 0.0 0.0 0.015418536164176573 0.0 8 0.0 0.0 0.0 0.01696038978059423 0.0 9 0.0 0.0 0.0 0.01696038978059423 0.0 10 0.0 0.0 0.0 0.021585950629847202 0.0 11 0.0 0.0 0.0 0.021585950629847202 0.0 12 0.0 0.0 0.0 0.021585950629847202 0.0 13 0.0 0.0 0.0 0.021585950629847202 0.0 14 0.0 0.0 0.0 0.02312780424626486 0.0 15 0.0 0.0 0.0 0.02312780424626486 0.0 16 0.0 0.0 0.0 0.024669657862682517 0.0 17 0.0 0.0 0.0 0.027753365095517832 0.0 18 0.0 0.0 0.0 0.032378925944770806 0.0 19 0.0 0.0 0.0 0.032378925944770806 0.0 20 0.0 0.0 0.0 0.03546263317760612 0.0 21 0.0 0.0 0.0 0.04008819402685909 0.0 22 0.0 0.0 0.0 0.04471375487611206 0.0 23 0.0 0.0 0.0 0.05088116934178269 0.0 24 0.0 0.0 0.0 0.052423022958200345 0.0 25 0.0 0.0 0.0 0.055506730191035664 0.0 26 0.0 0.0 0.0 0.05704858380745332 0.0 27 0.0 0.0 0.0 0.07709268082088286 0.0 28 0.0 0.0 0.0 0.10022048506714773 0.0 29 0.0 0.0 0.0 0.11718087484774195 0.0 30 0.0 0.0 0.0 0.13876682547758915 0.0 31 0.0 0.0 0.0 0.18656428758653654 0.0 32 0.0 0.0 0.0 0.22202692076414265 0.0 33 0.0 0.0 0.0 0.24978028585966047 0.0 34 0.0 0.0 0.0 0.3068288696671138 0.0 35 0.0 0.0 0.0 0.3900889649536673 0.0 36 0.0 0.0 0.0 0.5180628151163329 0.0 37 0.0 0.0 0.0 0.7863453443730052 0.0 38 0.0 0.0 0.0 1.1224694327520546 0.0 39 0.0 0.0 0.0 1.5248932266370632 0.0 40 0.0 0.0 0.0 1.9658633609325131 0.0 41 0.0 0.0 0.0 2.5085958339115284 0.0 42 0.0 0.0 0.0 3.079081671986062 0.0 43 0.0 0.0 0.0 3.6418582419785066 0.0 44 0.0 0.0 0.0 4.2108022264366225 0.0 45 0.0 0.0 0.0 4.842962209167862 0.0 46 0.0 0.0 0.0 5.39032024299613 0.0 47 0.0 0.0 0.0 5.9793083244676755 0.0 48 0.0 0.0 0.0 6.5482523089257905 0.0 49 0.0 0.0 0.0 7.115654439767488 0.0 50 0.0 0.0 0.0 7.642968376582327 0.0 51 0.0 0.0 0.0 8.228872750821036 0.0 52 0.0 0.0 0.0 8.862574587168695 0.0 53 0.0 0.0 0.0 9.41147447461338 0.0 54 0.0 0.0 0.0 9.958832508441649 0.0 55 0.0 0.0 0.0 10.466102348243059 0.0 56 0.0 0.0 0.0 11.001125553139985 0.0 57 0.0 0.0 0.0 11.47139090614737 0.0 58 0.0 0.0 0.0 11.986370014030868 0.0 59 0.0 0.0 0.0 12.479763171284517 0.0 60 0.0 0.0 0.0 12.894521794100868 0.0 61 0.0 0.0 0.0 13.378663829656013 0.0 62 0.0 0.0 0.0 13.825801378417133 0.0 63 0.0 0.0 0.0 14.405538338190173 0.0 64 0.0 0.0 0.0 14.909724470758746 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACA 65 0.0 59.230774 1 TTAATAC 25 2.3741944E-4 55.999996 3 CCAGGGA 20 0.0069226427 52.5 3 TTTCAGC 20 0.0069226427 52.5 33 GCCGAGC 20 0.0069226427 52.5 67 TCTTTAT 35 2.0655536E-5 50.0 1 ATAACAC 35 2.0655536E-5 50.0 4 CTTTATA 35 2.0655536E-5 50.0 2 TTTACAC 180 0.0 48.611115 2 TTACACA 200 0.0 47.249996 3 CTTATAA 40 4.546788E-5 43.75 1 CTTTACA 200 0.0 43.749996 1 CTTAATA 35 0.0012470287 40.0 2 TAATACA 35 0.0012470287 40.0 4 TAACACA 45 9.106207E-5 38.88889 5 TTTATAC 55 6.60202E-6 38.18182 3 TCAACTA 40 0.0024026963 35.0 5 CACAGAA 55 2.9626352E-4 31.818184 50 TATAACA 55 2.9626352E-4 31.818184 3 TCTTAAT 50 0.007161093 27.999998 1 >>END_MODULE