FastQCFastQC Report
Wed 25 May 2016
SRR1780437_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780437_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492457
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATA301.2869168E-436.72980518
ATACAGT301.3000835E-436.6663322
AAGCTTT250.002363335.16392543
ACGAATC401.8116905E-533.01647624
TACAGTT401.818005E-532.99969523
GACGAAT401.818005E-532.99969523
CGGATAC353.1828266E-431.48268919
CGAATCT353.2210385E-431.41870125
CAGTTAC353.222959E-431.4155061
GCCGTTT554.9067294E-628.03394112
TATCAGT407.076803E-427.4662386
CGTGCGA508.303309E-526.41318110
CGTTTAT551.5754494E-424.0364321
CTCTAGC1102.0372681E-1023.9705352
CCGTTTA651.7922433E-523.72344213
GTACAAT651.8163924E-523.6824591
TTAATAC651.8278539E-523.6632213
TATAGAT852.3747634E-723.265525
TGTCGGA500.002548123722.04461716
CGTTTAC703.165501E-522.04012714
Produced by FastQC (version 0.11.5)