FastQCFastQC Report
Wed 25 May 2016
SRR1780437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492457
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGT329836.697640606184906No Hit
ATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCTT262945.339349425432069RNA PCR Primer, Index 45 (95% over 23bp)
CTTTACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC74951.5219602929798948RNA PCR Primer, Index 45 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC42860.8703297952917718No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG37330.7580357269771777No Hit
TATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTCT25890.5257311805903866RNA PCR Primer, Index 45 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC22840.4637968391148872No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC15730.3194187512818378No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG9810.19920520979496686No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCC8430.17118245856998682No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCG7800.1583894634455394No Hit
CTTAACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC7000.14214439027163794RNA PCR Primer, Index 45 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGGTCATTTATCTCGTATGCCGTC6550.13300653661131834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT27050.042.2095544
GCCGTCT30150.037.869643
CTTATAA1000.037.417961
CTTTACA9700.036.759911
TTTACAC9700.036.741252
GGCTAAC250.002345910335.2169041
AGGATTC250.002351762735.19902835
TTACACA10550.034.1981053
CTATACA5500.033.6161351
TCGGATA353.2156365E-431.427737
CGGATAC353.2156365E-431.427738
GTCGGAT353.2156365E-431.427736
TAGGTTG502.3582106E-630.79914932
CTTTATA2000.029.6991792
TTATACA50550.029.6371632
ACTTGGT300.00573967729.33549916
GGTCCTG300.005742526629.33252137
CGCTCCC300.005742526629.33252119
GTTCGGG454.0654726E-529.33252137
TCAGTCG300.005742526629.33252136
Produced by FastQC (version 0.11.5)