FastQCFastQC Report
Wed 25 May 2016
SRR1780436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48346
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAC200.00691360352.4968952
TGAACCG200.00691360352.49689527
CCAGAAG200.00691360352.4968953
GTCCTAA305.706865E-446.8579371
CACAATT400.002397332834.9979320
GACGACG1558.403549E-720.32137970
CGACGAC1451.0819276E-519.30920269
ACATCTG4956.0411963E-58.48434669
CATCTGA4753.6350504E-48.10478470
CACATCT5552.287087E-47.567119668
ACACATC6251.11540096E-47.279569667
TACACAT6400.00116918456.56211266
Produced by FastQC (version 0.11.5)