##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780436_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48346 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.270653208124767 32.0 21.0 34.0 12.0 34.0 2 27.880672651305176 32.0 23.0 34.0 11.0 34.0 3 27.918959169321145 33.0 23.0 34.0 11.0 34.0 4 28.20760766144045 33.0 23.0 34.0 11.0 34.0 5 28.404997311049517 34.0 23.0 34.0 11.0 34.0 6 29.372026641294006 34.0 23.0 37.0 11.0 38.0 7 29.20584950150995 34.0 23.0 37.0 11.0 38.0 8 27.645906589997104 32.0 21.0 37.0 11.0 38.0 9 29.190357009887062 34.0 22.0 37.0 12.0 38.0 10-11 29.492564017705703 34.0 23.0 37.0 11.0 38.0 12-13 30.024500475737394 35.0 24.5 38.0 11.0 38.0 14-15 29.692901170727673 34.0 23.0 38.0 11.0 38.0 16-17 29.610050469532123 34.0 23.0 38.0 11.0 38.0 18-19 29.551865717949777 34.0 22.5 38.0 11.0 38.0 20-21 29.65003309477516 34.0 23.0 38.0 11.0 38.0 22-23 29.550552269060525 34.5 23.0 37.5 11.0 38.0 24-25 30.109357547677163 35.5 24.0 38.0 11.0 38.0 26-27 30.26500641211269 36.0 24.5 38.0 11.0 38.0 28-29 30.2951640259794 36.0 24.5 38.0 11.0 38.0 30-31 30.157303603193647 36.0 24.0 38.0 11.0 38.0 32-33 29.973275969056385 36.0 24.0 38.0 11.0 38.0 34-35 29.786404252678608 35.0 23.5 38.0 11.0 38.0 36-37 29.74236751747818 34.5 23.5 38.0 11.0 38.0 38-39 30.30238282381169 36.0 25.0 38.0 11.0 38.0 40-41 29.653011624539776 34.5 23.5 38.0 11.0 38.0 42-43 30.047170396723615 35.0 24.5 38.0 11.0 38.0 44-45 28.948940967194805 34.0 21.5 37.0 11.0 38.0 46-47 29.657272576841933 34.0 24.0 37.0 11.0 38.0 48-49 29.382916890745875 34.0 23.0 37.0 11.0 38.0 50-51 29.342324080585776 34.0 23.5 37.0 11.0 38.0 52-53 29.31543457576635 34.0 23.0 37.0 11.0 38.0 54-55 28.97072146609854 34.0 22.5 37.0 11.0 38.0 56-57 29.052238034170355 34.0 22.5 37.0 11.0 38.0 58-59 28.90310470359492 34.0 22.0 37.0 11.0 38.0 60-61 28.899650436437348 34.0 22.5 37.0 11.0 38.0 62-63 28.906041864890582 34.0 22.5 37.0 11.0 38.0 64-65 28.902360071153765 34.0 23.0 37.0 11.0 38.0 66-67 28.934058660488976 34.0 23.0 37.0 11.0 38.0 68-69 28.41595995532205 33.0 21.5 37.0 11.0 38.0 70-71 28.281833036859307 33.0 21.0 37.0 11.0 38.0 72-73 28.29063417862905 33.0 21.0 37.0 11.0 38.0 74-75 27.81355231042899 32.0 20.0 37.0 11.0 38.0 76 26.732366690108798 31.0 19.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.653527709631998 1101 2 -0.5507290615297009 1101 3 0.49936254143929304 1101 4 -1.5544149488501233 1101 5 -1.2979453125644298 1101 6 -1.2528842947911016 1101 7 -1.2685918315464235 1101 8 -0.7412101841988488 1101 9 0.02922946588495634 1101 10-11 -0.5094727241254233 1101 12-13 -1.1241879733235507 1101 14-15 -0.12052084300437471 1101 16-17 -2.8556989607261514 1101 18-19 -0.07518482795642001 1101 20-21 -0.7401781375762049 1101 22-23 0.48261503789281335 1101 24-25 -0.6372434753285106 1101 26-27 -1.3279796744500203 1101 28-29 -0.9586429740194049 1101 30-31 -0.8814384701605675 1101 32-33 -0.15240725420904866 1101 34-35 -0.8846502344564549 1101 36-37 0.016723635964400074 1101 38-39 -0.39296344116403503 1101 40-41 0.21840281195416722 1101 42-43 0.32314221930078446 1101 44-45 0.5140782718313694 1101 46-47 0.16717367256163485 1101 48-49 -0.5898572879905295 1101 50-51 -0.8061853799498984 1101 52-53 0.03349920524765082 1101 54-55 -1.8051412855332671 1101 56-57 -0.17548575001392663 1101 58-59 -0.058538382063986205 1101 60-61 0.17361966825412978 1101 62-63 -1.5771202002480038 1101 64-65 -0.2893826426994899 1101 66-67 -0.6325869985299413 1101 68-69 -0.2934439519168457 1101 70-71 0.7115817932542896 1101 72-73 -1.1558643811526181 1101 74-75 -0.16215298513176535 1101 76 -0.22720506686285447 1102 1 0.3208836439858942 1102 2 -1.2169477476924797 1102 3 -1.3571045051148403 1102 4 -0.8299767164019975 1102 5 0.5201387644165401 1102 6 0.3855954385709026 1102 7 0.37408099933197647 1102 8 0.8650687105436532 1102 9 -1.1063473538118487 1102 10-11 -0.7536400179647984 1102 12-13 -4.265301832048419 1102 14-15 -2.024025842466802 1102 16-17 -3.065622624848288 1102 18-19 -2.3323614756287085 1102 20-21 -1.1720134241045344 1102 22-23 -2.397048439152666 1102 24-25 -1.665681277715347 1102 26-27 -7.066851838733431 1102 28-29 -1.6633790239065114 1102 30-31 -1.4657842394326863 1102 32-33 -5.704525306031432 1102 34-35 -1.9491594270163937 1102 36-37 -1.2952858253838464 1102 38-39 -0.20801021032863787 1102 40-41 -1.4340270342989179 1102 42-43 -1.4005702847310388 1102 44-45 -6.371364153714271 1102 46-47 -1.1360732901305468 1102 48-49 -0.713714937705614 1102 50-51 -5.730198926880334 1102 52-53 -2.512544230942005 1102 54-55 -0.7198009995425139 1102 56-57 -1.7364469369830715 1102 58-59 -0.24185200425515063 1102 60-61 -1.3096885781709844 1102 62-63 -4.0611541750894204 1102 64-65 -1.752074826335658 1102 66-67 -0.394682472134928 1102 68-69 -6.164103020834165 1102 70-71 -1.793310153848168 1102 72-73 -0.11834153414692139 1102 74-75 -6.039558640437647 1102 76 -4.002605561433331 1103 1 1.407975817278814 1103 2 0.7126297170962523 1103 3 1.3010342005367903 1103 4 0.8250420035124257 1103 5 1.3249167997169486 1103 6 -0.10620345606272252 1103 7 1.6433803396778472 1103 8 2.592203672212399 1103 9 1.4493666772097242 1103 10-11 1.4421168014325971 1103 12-13 -0.11384881011937509 1103 14-15 1.107592984418563 1103 16-17 -0.23017958687360363 1103 18-19 0.6989720954178225 1103 20-21 0.20996197257936444 1103 22-23 1.168961544801693 1103 24-25 1.4047412574959175 1103 26-27 0.5895485891503256 1103 28-29 1.4559381449292879 1103 30-31 1.2478010698916115 1103 32-33 -2.5248586987930643 1103 34-35 0.9891539978365209 1103 36-37 1.365259726407917 1103 38-39 1.4888608896891888 1103 40-41 1.9621220118768683 1103 42-43 1.6866556022363355 1103 44-45 -2.415890807306713 1103 46-47 1.5419441817895816 1103 48-49 1.3820240532018566 1103 50-51 1.4785281189388861 1103 52-53 1.1925725456670158 1103 54-55 2.019769333850249 1103 56-57 1.8200693760852147 1103 58-59 1.7839101636892245 1103 60-61 2.1416842155533864 1103 62-63 1.4929107206139136 1103 64-65 1.6280731501307315 1103 66-67 0.46242218110094413 1103 68-69 -1.130674602221223 1103 70-71 1.7111469686335568 1103 72-73 2.7102821064307214 1103 74-75 0.29240784200129255 1103 76 0.9840230711214915 1104 1 -0.9469037253916639 1104 2 0.6126297170962509 1104 3 0.2214506970659862 1104 4 1.2109137879142224 1104 5 -0.3536458789273951 1104 6 0.13593619277353497 1104 7 2.1555894046023063 1104 8 3.112865166699944 1104 9 0.6063287025261808 1104 10-11 0.08429728326191821 1104 12-13 -0.18220324049912406 1104 14-15 -0.005943152780293559 1104 16-17 -0.059171011945061025 1104 18-19 1.0409688287783752 1104 20-21 1.1576549084715673 1104 22-23 -0.2929821056678854 1104 24-25 0.48538641878624134 1104 26-27 0.5725620640380349 1104 28-29 -0.4837106913304119 1104 30-31 -1.8743712453390913 1104 32-33 -1.11963207568159 1104 34-35 0.3820082240513045 1104 36-37 0.15260149855981808 1104 38-39 -0.29717422668483806 1104 40-41 0.31342866765473687 1104 42-43 0.9557450264911331 1104 44-45 -3.2540084063267187 1104 46-47 0.3117685999194322 1104 48-49 1.1792882426669422 1104 50-51 1.3521500054231659 1104 52-53 0.29161297033014577 1104 54-55 0.6792221717432625 1104 56-57 0.4756839126307426 1104 58-59 0.594404242905231 1104 60-61 0.7744322759862143 1104 62-63 1.2418898957874553 1104 64-65 0.5992654735280389 1104 66-67 1.3664033979241346 1104 68-69 -3.1481102902571543 1104 70-71 -0.5952638112766095 1104 72-73 0.4895185294605291 1104 74-75 -0.909776723127333 1104 76 -1.064486524631878 1105 1 -0.5387897337035561 1105 2 0.30465425697355286 1105 3 -0.34993263956571496 1105 4 0.8821981734277777 1105 5 1.0302052985909107 1105 6 1.054004666947293 1105 7 1.6495929668425617 1105 8 0.1617074386176185 1105 9 1.1658379049801688 1105 10-11 1.1288450766519844 1105 12-13 -1.5177860625850137 1105 14-15 -0.41083133801678784 1105 16-17 0.9374448598142884 1105 18-19 1.6344578452042633 1105 20-21 0.11494760587904551 1105 22-23 0.1403446309242362 1105 24-25 0.4231461654713762 1105 26-27 0.14612232526898694 1105 28-29 -1.3135345583401055 1105 30-31 0.3381559662183946 1105 32-33 0.6488618883001998 1105 34-35 1.4892316556760825 1105 36-37 1.5366505783144184 1105 38-39 1.0832928225928171 1105 40-41 0.9257975574231914 1105 42-43 1.5955629564337386 1105 44-45 2.1373630561708055 1105 46-47 1.566251085571519 1105 48-49 0.91805729075719 1105 50-51 0.04589629475623269 1105 52-53 -1.179117288921784 1105 54-55 -0.36688904931353505 1105 56-57 0.48652895517972183 1105 58-59 1.1394665821096623 1105 60-61 0.6838524224338727 1105 62-63 0.34984556568652536 1105 64-65 0.5193327602883784 1105 66-67 0.7966032791827935 1105 68-69 1.1679197908827597 1105 70-71 1.2438020901680744 1105 72-73 0.08368041348981592 1105 74-75 -0.8626166202181729 1105 76 -0.7918564039115132 1106 1 -0.12892157027841478 1106 2 -0.07815042474771872 1106 3 -1.119066347088669 1106 4 -1.4946455806513264 1106 5 -1.1621107611053887 1106 6 -1.2266810572836597 1106 7 0.009032552623356338 1106 8 0.16653709532039684 1106 9 0.6588109720297268 1106 10-11 0.35144438918733556 1106 12-13 -0.9616358646125995 1106 14-15 -0.12921013973980067 1106 16-17 0.26061850990348745 1106 18-19 -0.9353981353030676 1106 20-21 -1.0626836868155536 1106 22-23 -1.5632406277452127 1106 24-25 4.8593834697996385E-4 1106 26-27 -0.10019016657280844 1106 28-29 -1.3395102795253315 1106 30-31 -1.0144307282949399 1106 32-33 0.2866593908020505 1106 34-35 -0.5388108104945672 1106 36-37 -0.47363963619195815 1106 38-39 -0.4276935586454904 1106 40-41 0.4070228108713927 1106 42-43 -0.7233901828431151 1106 44-45 1.2556461349681776 1106 46-47 0.64002300394597 1106 48-49 0.09075015069714354 1106 50-51 0.5328866789532505 1106 52-53 -0.7152962037718922 1106 54-55 -0.9172088509060146 1106 56-57 -1.0462561468522154 1106 58-59 -0.9745959858753679 1106 60-61 -0.490015218866219 1106 62-63 0.770830641612207 1106 64-65 0.9356187107735217 1106 66-67 1.058144418285611 1106 68-69 0.8694600597771611 1106 70-71 0.48563300867305514 1106 72-73 -0.08218131039305376 1106 74-75 -2.1195456547219322 1106 76 -0.8997187369402759 1107 1 -0.8243230802303714 1107 2 0.012629717096253046 1107 3 -0.3785493029340117 1107 4 0.5722041104948659 1107 5 -0.1794523305403004 1107 6 0.09722651535417981 1107 7 -1.1347331760428538 1107 8 -0.6032638655581195 1107 9 -0.9227035555383374 1107 10-11 -1.228605942544533 1107 12-13 -1.2693000146926714 1107 14-15 -1.4511044431028743 1107 16-17 -0.8140097216224831 1107 18-19 0.10548495781063494 1107 20-21 -0.022990252818757995 1107 22-23 -0.7542724282485302 1107 24-25 -1.446871645729889 1107 26-27 -1.130663742413578 1107 28-29 -2.1417752074594425 1107 30-31 -1.5130809227584479 1107 32-33 0.11262598883453734 1107 34-35 -0.037346614658371635 1107 36-37 -1.5506243078917983 1107 38-39 -0.061690355717097134 1107 40-41 0.18762221604183438 1107 42-43 -0.42812594125080494 1107 44-45 -0.683040664391239 1107 46-47 -0.35919914201605607 1107 48-49 0.6341269523443636 1107 50-51 -0.1381725752219971 1107 52-53 0.020645228394659654 1107 54-55 -0.12400363470834819 1107 56-57 0.014393590050094218 1107 58-59 0.08795263000200393 1107 60-61 -0.5320193369170099 1107 62-63 -0.5839165558254464 1107 64-65 -0.21686355873002583 1107 66-67 0.22446791405316802 1107 68-69 0.5905993871622002 1107 70-71 -0.18236058547016043 1107 72-73 -0.7588685673136659 1107 74-75 -0.4936476908692704 1107 76 -1.5160994278576858 1108 1 -1.7373503755157316 1108 2 -0.941216436749901 1108 3 -0.3051001714203636 1108 4 -0.2847437803239927 1108 5 -0.4942165985303717 1108 6 -0.7513673638848601 1108 7 1.089501728837213 1108 8 -0.35351200451593456 1108 9 0.3909440871415626 1108 10-11 -0.34938758026165573 1108 12-13 0.1421557338598518 1108 14-15 -0.06803992697384231 1108 16-17 -1.0976739068995691 1108 18-19 0.34644856409682134 1108 20-21 0.3644166950720624 1108 22-23 -1.5697190783726 1108 24-25 -1.4414125886579292 1108 26-27 -1.0776860749197787 1108 28-29 -0.2583591611401701 1108 30-31 0.031191202965288767 1108 32-33 -0.6602855082705084 1108 34-35 -0.7378015360810366 1108 36-37 0.24683226779058742 1108 38-39 -0.16535040534488843 1108 40-41 0.7535858223280201 1108 42-43 0.6490866311230583 1108 44-45 0.19774924462447885 1108 46-47 0.6266569373884145 1108 48-49 -0.34201035121229495 1108 50-51 -0.22270524685144366 1108 52-53 -0.27708016449202333 1108 54-55 -0.08080677780181134 1108 56-57 0.36011319302776457 1108 58-59 -2.389694185548038 1108 60-61 0.613162631652056 1108 62-63 0.33090974690408004 1108 64-65 -0.13032509719156593 1108 66-67 -0.09583812399480607 1108 68-69 1.706066715532753 1108 70-71 1.6641654691204124 1108 72-73 0.27665666014704726 1108 74-75 1.0238667839032694 1108 76 0.09932654401989893 1109 1 0.4646309764656209 1109 2 -0.684339979873446 1109 3 0.2855758192556266 1109 4 0.05803011244989875 1109 5 0.9597461054323304 1109 6 1.9771874146699204 1109 7 -0.5480274086919259 1109 8 0.21863251762858482 1109 9 1.8426923388898153 1109 10-11 1.1779434220693474 1109 12-13 -0.0943244526203344 1109 14-15 -0.9778884118223239 1109 16-17 -1.698955958181621 1109 18-19 -0.2678288251805654 1109 20-21 0.48219058784595603 1109 22-23 0.16635318269966248 1109 24-25 0.49868065143531126 1109 26-27 0.13688268964898143 1109 28-29 0.007491654710644724 1109 30-31 0.37518887196295836 1109 32-33 0.5535839555989561 1109 34-35 -0.06589011416961554 1109 36-37 0.24351058946890802 1109 38-39 0.15219038719590117 1109 40-41 0.6385508572930547 1109 42-43 0.23824747028389837 1109 44-45 -0.7646633427881078 1109 46-47 -0.6141356034041259 1109 48-49 -0.5038984631004055 1109 50-51 0.5656397414935448 1109 52-53 0.06033242292056329 1109 54-55 0.5056151336200969 1109 56-57 0.19513650305107433 1109 58-59 -0.861314232168084 1109 60-61 0.40727685076627296 1109 62-63 -1.0588921178977841 1109 64-65 -0.33807567994214693 1109 66-67 -0.20084977900646095 1109 68-69 0.40653291599895525 1109 70-71 0.2969160518709941 1109 72-73 -1.3169330834426987 1109 74-75 0.6143679494044356 1109 76 -1.1520720573786996 1110 1 -0.04181604407536099 1110 2 -0.25716894062187023 1110 3 -0.27618948479155847 1110 4 -0.2593178639584792 1110 5 -1.356503640347615 1110 6 0.011883367486678509 1110 7 0.3559790993414289 1110 8 1.874804980512458 1110 9 -1.3762216330442918 1110 10-11 -1.9530917619859736 1110 12-13 -1.5452904888212693 1110 14-15 -1.5823016719402858 1110 16-17 -1.065622624848288 1110 18-19 -0.4115750118797763 1110 20-21 -1.7989157778241704 1110 22-23 -0.9773726663953148 1110 24-25 -0.8033124337792508 1110 26-27 -3.0104429153947407 1110 28-29 -0.48323439776037347 1110 30-31 -1.3046158870364302 1110 32-33 -0.052171586398198855 1110 34-35 -1.0264351619629828 1110 36-37 -1.3319622598294423 1110 38-39 -0.31990641980023327 1110 40-41 -1.2334645992861617 1110 42-43 -0.5071257247537275 1110 44-45 0.018842860181184307 1110 46-47 -1.4817581374696154 1110 48-49 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2.5340149292944183 2116 46-47 0.7215832876339157 2116 48-49 1.356707597505654 2116 50-51 1.5470710761163708 2116 52-53 3.1393137253061205 2116 54-55 1.1998124256897285 2116 56-57 1.3615452715875023 2116 58-59 0.09632599994023394 2116 60-61 0.18551669602739906 2116 62-63 1.9481356061512187 2116 64-65 1.3256896327593353 2116 66-67 0.2248110849522753 2116 68-69 1.5822260172239702 2116 70-71 -1.5817428778517666 2116 72-73 1.6288115974083652 2116 74-75 0.46201462896600987 2116 76 1.096735163768944 2117 1 -1.4983393865047425 2117 2 -0.029128524661988564 2117 3 0.7168778505576583 2117 4 -1.177600923181135 2117 5 -0.6563045106182841 2117 6 -1.5691329316504259 2117 7 -1.9233187839833015 2117 8 -1.0485669495708798 2117 9 0.5118232080206866 2117 10-11 1.0338541779801034 2117 12-13 1.044170385874505 2117 14-15 -0.33589706983098466 2117 16-17 1.3959158366901718 2117 18-19 0.4430602857085404 2117 20-21 0.9784276840830515 2117 22-23 0.28879740514388175 2117 24-25 0.9212247739794357 2117 26-27 2.5019842547505498 2117 28-29 1.6601390073580014 2117 30-31 1.0856783824524712 2117 32-33 1.9944763964913967 2117 34-35 0.19168564340614225 2117 36-37 -1.8122336662753469 2117 38-39 1.322286957292473 2117 40-41 -1.3956815769393778 2117 42-43 -0.6357119036754746 2117 44-45 0.87403129590653 2117 46-47 -0.32389251316103795 2117 48-49 -0.24768800688995185 2117 50-51 1.3120932879470928 2117 52-53 1.6084509710391117 2117 54-55 -0.29738186937690614 2117 56-57 0.6609373688519398 2117 58-59 0.4926318217779695 2117 60-61 2.2757084191978443 2117 62-63 0.35975590075023334 2117 64-65 -0.6509294927959601 2117 66-67 -2.419098197254879 2117 68-69 0.317788327254366 2117 70-71 0.9965830515379928 2117 72-73 1.096253729744113 2117 74-75 0.28256641760290435 2117 76 0.31452800922136603 2118 1 -0.7071305952959506 2118 2 0.9708714753380114 2118 3 0.5630316967115014 2118 4 0.23998149440128103 2118 5 0.0250141707003948 2118 6 0.06822970571220921 2118 7 0.7360218753573591 2118 8 -3.543072444076376 2118 9 -1.0815833853859083 2118 10-11 -1.339772195646269 2118 12-13 0.7529615946657131 2118 14-15 -1.0886443225782365 2118 16-17 0.2915202322945696 2118 18-19 -1.7602364175881604 2118 20-21 -2.9061877005323353 2118 22-23 -0.21120259485611825 2118 24-25 -0.8919620392073817 2118 26-27 1.1283578811241775 2118 28-29 0.43486428208327155 2118 30-31 0.28348058025466827 2118 32-33 -0.1703587683437675 2118 34-35 0.8400372917577918 2118 36-37 1.0064476524059707 2118 38-39 0.19041882542434152 2118 40-41 -1.131945313203115 2118 42-43 -0.9653822333458066 2118 44-45 -0.30179287991764525 2118 46-47 -0.8513650406335636 2118 48-49 -2.2641715233734665 2118 50-51 -0.7373572615034583 2118 52-53 -1.6607797981916583 2118 54-55 -1.0830961550911908 2118 56-57 0.6334648413794106 2118 58-59 0.5750494041955534 2118 60-61 -1.4825333390439148 2118 62-63 0.10700864800298149 2118 64-65 -0.43114927301574113 2118 66-67 -1.259757537914222 2118 68-69 1.8232828327488697 2118 70-71 -1.0253949704400291 2118 72-73 -2.3817682482778615 2118 74-75 -1.0196313845948985 2118 76 -0.3667906720973164 2119 1 0.9100855219201662 2119 2 1.573740828207363 2119 3 1.0239596576394625 2119 4 1.0966664760862628 2119 5 0.42565519634142035 2119 6 2.1610257985083017 2119 7 -1.3207546814191993 2119 8 1.2885820125780825 2119 9 -0.3131157419182635 2119 10-11 0.39473635136227614 2119 12-13 0.8344634261675417 2119 14-15 -0.651373260307178 2119 16-17 1.0871551529294905 2119 18-19 -0.40835912821087206 2119 20-21 -0.8791730485176821 2119 22-23 1.0638279301744085 2119 24-25 0.41168570194036036 2119 26-27 1.1060746088409026 2119 28-29 2.08085024056923 2119 30-31 1.0530929123669992 2119 32-33 0.8995435515585513 2119 34-35 1.3791856434061422 2119 36-37 -0.1751762792179612 2119 38-39 0.774286346791861 2119 40-41 -0.22344409220189476 2119 42-43 -0.7301868731504442 2119 44-45 -1.245092635717402 2119 46-47 -0.8017618660303896 2119 48-49 -0.45579240249434605 2119 50-51 0.04973065058445414 2119 52-53 -0.7231271730390318 2119 54-55 -1.3505717350667723 2119 56-57 0.24526276567733518 2119 58-59 -0.16106170691555732 2119 60-61 0.35942331541274086 2119 62-63 -0.8987911077967752 2119 64-65 -0.10575244761891511 2119 66-67 -1.1460517992084824 2119 68-69 -0.7298307203646814 2119 70-71 -1.6798516248966848 2119 72-73 -2.1891553056649187 2119 74-75 -1.5044451819086966 2119 76 0.7403521850455412 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 5.0 13 80.0 14 317.0 15 651.0 16 1179.0 17 1456.0 18 1735.0 19 1555.0 20 1536.0 21 1344.0 22 1187.0 23 1171.0 24 1247.0 25 1302.0 26 1377.0 27 1540.0 28 1669.0 29 1900.0 30 2211.0 31 2585.0 32 2925.0 33 3497.0 34 4253.0 35 4869.0 36 4935.0 37 1817.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.664169164169166 21.414002664002666 10.110722610722611 36.81110556110556 2 17.02933024448765 27.31973689653746 30.51751954660158 25.13341331237331 3 15.722086625573988 24.421875646382325 27.309394779299218 32.54664294874447 4 12.16232987217143 19.58590162578083 31.71306829934224 36.5387002027055 5 11.320481528978613 38.78707649029909 30.728498738261695 19.163943242460597 6 22.181773052579324 34.02556571381293 16.313655731601372 27.47900550200637 7 21.43714061142597 35.070119554875276 19.3314855417201 24.161254291978654 8 18.73577958879742 32.86311173623464 17.51127290778968 30.88983576717826 9 19.734828114011503 20.853845199189177 19.15773797211765 40.25358871468167 10-11 20.865221528151242 26.38998055681959 25.558474330865018 27.18632358416415 12-13 20.777313531626195 24.440491457411163 24.721797046291314 30.060397964671324 14-15 19.801017664336243 25.97733007901378 22.80436851032143 31.41728374632855 16-17 21.064824390849296 28.12745625284408 23.596574690770694 27.21114466553593 18-19 19.864104579489513 26.73230463740537 24.200554337483972 29.20303644562115 20-21 19.13188267902205 27.61448723782733 24.25847019401812 28.995159889132506 22-23 21.583998676208992 26.962933851818143 24.252264923675174 27.200802548297688 24-25 20.462913167583668 28.819343896082405 24.11988582302569 26.597857113308237 26-27 19.184627476937077 29.22475489182145 25.769453522525133 25.82116410871634 28-29 20.626318619947874 27.34662640135689 27.122202457287052 24.904852521408184 30-31 20.467050014478964 26.92570222976048 27.171844620030612 25.435403135729945 32-33 18.982956190791377 29.374715591775946 26.16969345964506 25.472634757787617 34-35 20.01303106772018 28.607330492698463 25.818061473544862 25.561576966036487 36-37 19.86100194431804 27.603110908865265 26.08592231001531 26.44996483680139 38-39 20.137136474579076 26.763330989120092 27.343523766185413 25.756008770115418 40-41 19.29115128449096 26.50477805816407 27.25458155793654 26.94948909940843 42-43 18.601332064700284 26.2400198568651 27.891655979812185 27.266992098622428 44-45 18.359326521325446 26.622678194680017 28.139866793529972 26.87812849046457 46-47 18.827824432217763 26.912257477350764 27.71894262193356 26.540975468497912 48-49 18.151449964836804 27.774790055020066 27.156331444173247 26.917428535969883 50-51 18.11111570760766 27.269060522070077 26.88019691391222 27.739626856410045 52-53 17.662267819468003 26.803665246349233 27.243205228974475 28.290861705208293 54-55 17.209283084433043 27.6775741529806 26.652670334670916 28.460472427915445 56-57 17.85669962354693 27.139784056592063 26.697141438795352 28.30637488106565 58-59 18.086294626235883 26.761262565672446 26.333098912009266 28.819343896082405 60-61 17.7522442394407 26.473751706449345 26.39204898026724 29.381955073842718 62-63 18.041823522111446 26.653704546394735 26.329996276837793 28.974475654656022 64-65 18.34484755719191 27.265957886898605 25.06825797377239 29.320936582137097 66-67 18.37794233235428 27.92475075497456 24.284325487113723 29.41298142555744 68-69 18.177305257932407 27.49141604269226 24.529433665660033 29.801845033715303 70-71 19.07603524593555 27.37248169445249 24.789020808339885 28.76246225127208 72-73 18.974682497000785 26.801596822901587 24.47358623257353 29.750134447524097 74-75 19.567285814751997 26.615438712613248 24.57390476978447 29.243370702850285 76 19.83824928639391 25.00723948206677 25.13341331237331 30.021097919166014 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 1.0 12 2.0 13 1.5 14 1.0 15 1.0 16 1.5 17 2.0 18 3.0 19 9.5 20 19.0 21 23.0 22 23.0 23 28.5 24 49.0 25 64.0 26 91.5 27 137.0 28 209.5 29 264.0 30 303.0 31 404.5 32 547.5 33 628.0 34 720.0 35 939.5 36 1162.0 37 1257.0 38 1415.5 39 1773.0 40 2139.0 41 2478.5 42 2651.0 43 2833.5 44 3097.5 45 3233.5 46 3288.0 47 3299.0 48 3246.5 49 3035.0 50 2887.0 51 2788.5 52 2556.0 53 2247.0 54 2072.0 55 1916.5 56 1587.5 57 1302.5 58 1191.0 59 1090.0 60 895.0 61 688.5 62 576.0 63 500.0 64 381.5 65 312.5 66 242.5 67 199.0 68 176.0 69 115.5 70 76.0 71 74.0 72 66.5 73 44.5 74 26.5 75 23.0 76 23.5 77 20.0 78 15.0 79 14.0 80 11.5 81 6.5 82 7.5 83 11.0 84 8.5 85 6.0 86 6.0 87 6.0 88 4.5 89 4.0 90 3.5 91 1.5 92 1.0 93 2.0 94 2.5 95 1.5 96 1.0 97 1.5 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6163901873991643 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 48346.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.18181442104827 #Duplication Level Percentage of deduplicated Percentage of total 1 90.01262687581954 76.67438878087121 2 6.160458452722064 10.49518057336698 3 2.1271429265212958 5.435816820419476 4 0.8741683259676558 2.9785297646134117 5 0.3836627652858045 1.63405452364208 6 0.20882910009227332 1.0673064989864725 7 0.09227332329658589 0.5502006370744219 8 0.05342139769802341 0.3640425267860836 9 0.03156718954883201 0.2420055433748397 >10 0.055849643047933564 0.5584743308650146 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0020684234476482026 0.0 2 0.0 0.0 0.0 0.0020684234476482026 0.0 3 0.0 0.0 0.0 0.0020684234476482026 0.0 4 0.0 0.0 0.0 0.004136846895296405 0.0 5 0.0 0.0 0.0 0.004136846895296405 0.0 6 0.0 0.0 0.0 0.006205270342944607 0.0 7 0.0 0.0 0.0 0.006205270342944607 0.0 8 0.0 0.0 0.0 0.006205270342944607 0.0 9 0.0 0.0 0.0 0.00827369379059281 0.0 10 0.0 0.0 0.0 0.00827369379059281 0.0 11 0.0 0.0 0.0 0.010342117238241013 0.0 12 0.0 0.0 0.0 0.010342117238241013 0.0 13 0.0 0.0 0.0 0.010342117238241013 0.0 14 0.0 0.0 0.0 0.010342117238241013 0.0 15 0.0 0.0 0.0 0.012410540685889215 0.0 16 0.0 0.0 0.0 0.01654738758118562 0.0 17 0.0 0.0 0.0 0.01654738758118562 0.0 18 0.0 0.0 0.0 0.018615811028833824 0.0 19 0.0 0.0 0.0 0.020684234476482027 0.0 20 0.0 0.0 0.0 0.020684234476482027 0.0 21 0.0 0.0 0.0 0.02482108137177843 0.0 22 0.0 0.0 0.0 0.02482108137177843 0.0 23 0.0 0.0 0.0 0.028957928267074835 0.0 24 0.0 0.0 0.0 0.028957928267074835 0.0 25 0.0 0.0 0.0 0.035163198610019444 0.0 26 0.0 0.0 0.0 0.04136846895296405 0.0 27 0.0 0.0 0.0 0.043436892400612256 0.0 28 0.0 0.0 0.0 0.05584743308650147 0.0 29 0.0 0.0 0.0 0.06412112687709427 0.0 30 0.0 0.0 0.0 0.0806685144582799 0.0 31 0.0 0.0 0.0 0.10548959583005833 0.0 32 0.0 0.0 0.0 0.11376328962065115 0.0 33 0.0 0.0 0.0 0.15306333512596698 0.0 34 0.0 0.0 0.0 0.17374756960244903 0.0 35 0.0 0.0 0.0 0.2399371199271915 0.0 36 0.0 0.0 0.0 0.34749513920489805 0.0 37 0.0 0.0 0.0 0.4716005460637902 0.0 38 0.0 0.0 0.0 0.6391428453232946 0.0 39 0.0 0.0 0.0 0.8356430728498738 0.0 40 0.0 0.0 0.0 1.1479750134447524 0.0 41 0.0 0.0 0.0 1.5451123153932074 0.0 42 0.0 0.0 0.0 2.008439167666405 0.0 43 0.0 0.0 0.0 2.4696975964919536 0.0 44 0.0 0.0 0.0 2.912340214288669 0.0 45 0.0 0.0 0.0 3.3694617962189217 0.0 46 0.0 0.0 0.0 3.8844992346833243 0.0 47 0.0 0.0 0.0 4.409878790385968 0.0 48 0.0 0.0 0.0 4.790468704753237 0.0 49 0.0 0.0 0.0 5.1317585736151905 0.0 50 0.0 0.0 0.0 5.506143217639515 0.0 51 0.0 0.0 0.0 5.9322384478550445 0.0 52 0.0 0.0 0.0 6.343854713937037 0.0 53 0.0 0.0 0.0 6.856823728953791 0.0 54 0.0 0.0 0.0 7.365655897075249 0.0 55 0.0 0.0 0.0 7.839324866586687 0.0 56 0.0 0.0 0.0 8.315062259545774 0.0 57 0.0 0.0 0.0 8.794936499400157 0.0 58 0.0 0.0 0.0 9.214826459272743 0.0 59 0.0 0.0 0.0 9.653332230174161 0.0 60 0.0 0.0 0.0 10.052537955570264 0.0 61 0.0 0.0 0.0 10.457948951309312 0.0 62 0.0 0.0 0.0 10.879907334629545 0.0 63 0.0 0.0 0.0 11.310139411740371 0.0 64 0.0 0.0 0.0 11.77346626401357 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTACAC 20 0.006913603 52.496895 2 TGAACCG 20 0.006913603 52.496895 27 CCAGAAG 20 0.006913603 52.496895 3 GTCCTAA 30 5.706865E-4 46.857937 1 CACAATT 40 0.0023973328 34.99793 20 GACGACG 155 8.403549E-7 20.321379 70 CGACGAC 145 1.0819276E-5 19.309202 69 ACATCTG 495 6.0411963E-5 8.484346 69 CATCTGA 475 3.6350504E-4 8.104784 70 CACATCT 555 2.287087E-4 7.5671196 68 ACACATC 625 1.11540096E-4 7.2795696 67 TACACAT 640 0.0011691845 6.562112 66 >>END_MODULE