FastQCFastQC Report
Wed 25 May 2016
SRR1780436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48346
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTT20784.298183924212965TruSeq Adapter, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT14302.9578455301369297No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC7571.5657965498696893No Hit
CTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC2970.6143217639515162No Hit
TATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCT1920.39713730194845487No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG1420.29371612956604476No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGT910.18822653373598644No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG860.17788441649774542No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC740.15306333512596698No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC730.1509949116783188No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGATATCGTATGCCGTCTT690.142721217887726No Hit
CTTATAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG630.13031067720183676No Hit
CTTAACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTC590.12203698341124394No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGACCTCGTATGCCGT560.11583171306829934No Hit
ATACACATCTCCGAGCCCACGAGACGTGTGGTGAACTCGTATGCCGTCTT550.11376328962065113No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC550.11376328962065113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAC252.7999758E-670.000014
GGCTGGT150.002211987770.06
TTTACAC1000.059.5000042
CTTTACA1050.056.666671
TTGTGGC200.006911985652.549
AGCTTGA200.006911985652.552
TGTGGCA200.006911985652.550
GCTATAC200.006911985652.53
CGGGGAA200.006911985652.547
CTTATTC200.006911985652.554
ATCCATT200.006911985652.512
CTTTATA200.006911985652.52
TCCTTAT200.006911985652.552
CATCACT200.006911985652.514
TTACACA1250.047.6000023
TTAATAC451.6522463E-646.6666642
CTATACA802.7284841E-1143.751
ATATCGT350.001243865140.037
TTTATAC501.6863902E-435.0000043
CTTAATA601.19264005E-535.01
Produced by FastQC (version 0.11.5)