FastQCFastQC Report
Wed 25 May 2016
SRR1780435_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780435_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445758
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCTA250.002340657235.23226518
AGTACCA300.005718653629.35692417
ACGGAAA300.005731224529.3437424
AATTCGA407.010566E-427.50975224
CAGTTGT407.015169E-427.5066661
TTCGAAC407.0382236E-427.49123226
TCGAACA407.0382236E-427.49123227
CGACGTT508.271732E-526.42716814
TCGGTCA609.759062E-625.65848729
TCTATGG450.001399427124.43665329
ATACTGC450.001402174224.428436
AGCATAC651.816108E-523.68209833
GCGCTAT602.8489425E-422.02264219
CGTGCGA703.2034786E-522.00533310
ACCGTTA602.8684884E-422.00039338
CAGCATA500.002584365721.99051932
TCGCATA500.002586050221.98805242
TTCGGTC755.476128E-520.52678728
CGACAAT654.898807E-420.32402417
TGCTCTC654.9472624E-420.2943885
Produced by FastQC (version 0.11.5)