FastQCFastQC Report
Wed 25 May 2016
SRR1780435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780435_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445758
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGT250315.61537874810996No Hit
ATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCTT194454.362232422076553TruSeq Adapter, Index 18 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC61671.3834861068113191No Hit
CTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC32880.7376199641958192No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG28590.6413794031739195No Hit
TATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTCT19300.4329703561125095No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC15250.3421138824205062No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC11390.2555198111980043No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG9390.21065241678220023No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCC7800.17498283822163596No Hit
CTTATAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCG6540.14671637973967938No Hit
CTTAACACATCTCCGAGCCCACGAGACTACACCTCATCTCGTATGCCGTC5260.11800124731356476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT18300.042.1998444
GCCGTCT20850.037.03870843
CTTATAA900.036.681721
GCGAAGT250.002351645835.19865432
GCTACCG250.002351645835.19865420
TTTACAC6700.035.1329882
CTATACA4250.034.6965941
GCGAACT607.9890015E-932.99873728
CTTTACA7300.032.2598151
ACTTCGG353.2132346E-431.43089534
TTACACA7950.029.8856473
TCGTTGT300.00573909929.33550334
CATCGTT300.005742246829.3322132
CTTATAC41000.029.309591
TTATACA41050.029.1535762
CTCGTGG703.5814082E-828.2846348
CCTCGTG703.5814082E-828.2846347
AAAGCGA703.5814082E-828.28463425
AACTTCG407.0266944E-427.49894733
TTACCAG905.11136E-1026.88786116
Produced by FastQC (version 0.11.5)