FastQCFastQC Report
Wed 25 May 2016
SRR1780434_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780434_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64965
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT150.002216502669.9915463
GTCATTC200.006926003452.4936530
GGTCGGA200.006926003452.4936525
TATGGAC200.006926003452.493656
CTCTTCG200.006926003452.4936539
TGGCCAC200.006926003452.4936516
GAGACAA200.006926003452.4936512
CGAGGCA200.006926003452.4936524
GATATGG200.006926003452.493654
GACCTCA200.006926003452.4936567
GTCGGAG200.006926003452.4936526
AGGGGGG305.841785E-446.6610229
TGATATG350.00124778539.995163
AACCCAT350.00124778539.995164
TCTTTCA350.00124778539.995168
AGGAGAC450.004281418431.1073510
CATCGCT450.004281418431.1073537
GAGGGGG604.9368077E-429.163148
CCATCGC500.007165402627.99661436
CCAAGAC500.007165402627.99661463
Produced by FastQC (version 0.11.5)