##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780434_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64965 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.859031786346495 33.0 23.0 34.0 12.0 34.0 2 28.737951204494728 34.0 23.0 34.0 12.0 34.0 3 28.947002231971062 34.0 23.0 34.0 11.0 34.0 4 29.518925575309783 34.0 31.0 34.0 11.0 34.0 5 29.543677364734858 34.0 31.0 34.0 11.0 34.0 6 31.594904948818595 37.0 27.0 38.0 11.0 38.0 7 30.683752789963826 36.0 26.0 38.0 11.0 38.0 8 30.065558377587934 35.0 24.0 38.0 11.0 38.0 9 31.042068806280305 36.0 26.0 38.0 12.0 38.0 10-11 31.1753944431617 37.0 27.0 38.0 11.0 38.0 12-13 31.484160701916416 37.0 27.0 38.0 11.0 38.0 14-15 31.148333718155932 37.0 27.0 38.0 11.0 38.0 16-17 30.957684907257754 37.0 26.0 38.0 11.0 38.0 18-19 30.984337720310936 37.0 26.5 38.0 11.0 38.0 20-21 31.080720387901177 37.0 26.5 38.0 11.0 38.0 22-23 30.92341260678827 37.0 26.0 38.0 11.0 38.0 24-25 31.363988301393057 37.0 27.0 38.0 11.0 38.0 26-27 31.37812668359886 37.0 27.0 38.0 11.0 38.0 28-29 31.350573385669207 37.0 27.0 38.0 11.0 38.0 30-31 31.23637343184792 37.0 27.0 38.0 11.0 38.0 32-33 31.157877318556146 37.0 26.5 38.0 11.0 38.0 34-35 31.07230046948357 37.0 26.0 38.0 11.0 38.0 36-37 30.988386054029093 37.0 26.0 38.0 11.0 38.0 38-39 31.302278149772956 37.0 27.0 38.0 11.0 38.0 40-41 30.91829446625106 37.0 26.0 38.0 11.0 38.0 42-43 31.269522050334796 37.0 26.5 38.0 11.0 38.0 44-45 30.4494497036866 36.0 25.0 38.0 11.0 38.0 46-47 31.067059185715387 37.0 26.5 38.0 11.0 38.0 48-49 30.908889401985682 37.0 26.0 38.0 11.0 38.0 50-51 30.914084507042254 36.5 26.5 38.0 11.0 38.0 52-53 30.838166705148925 37.0 26.0 38.0 11.0 38.0 54-55 30.571800200107752 36.0 25.5 38.0 11.0 38.0 56-57 30.569260370968983 36.5 25.5 38.0 11.0 38.0 58-59 30.474871084430077 36.5 25.0 38.0 11.0 38.0 60-61 30.524613253290234 36.5 25.0 38.0 11.0 38.0 62-63 30.577595628415303 36.0 25.0 38.0 11.0 38.0 64-65 30.57380127761102 36.0 25.0 38.0 11.0 38.0 66-67 30.628738551527746 36.5 25.0 38.0 11.0 38.0 68-69 30.142191949511275 36.0 24.5 38.0 11.0 38.0 70-71 29.994566304933425 36.0 24.0 38.0 11.0 38.0 72-73 30.005048872469793 36.0 24.0 38.0 11.0 38.0 74-75 29.608527668744706 35.5 23.5 38.0 11.0 38.0 76 28.783006234126066 34.0 22.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.087012115870472 1101 2 -1.499362622482348 1101 3 -0.7473512496318762 1101 4 -1.9256859244686773 1101 5 -1.0441854842171807 1101 6 -3.9707066993141 1101 7 -0.6655243735656384 1101 8 -1.1781550157514182 1101 9 -0.9730013420394918 1101 10-11 -1.8045411405578307 1101 12-13 -1.2013902857409988 1101 14-15 -0.9689881335415542 1101 16-17 -2.6509440830268467 1101 18-19 -1.5413274928460226 1101 20-21 -2.1386890615476837 1101 22-23 -1.74328796229225 1101 24-25 -2.9184372783467403 1101 26-27 -1.9574825985253952 1101 28-29 -2.1097577782356964 1101 30-31 -2.3220677813857407 1101 32-33 -1.1156245932278601 1101 34-35 -1.0533080864741429 1101 36-37 -0.9034151602588167 1101 38-39 -1.597927353317587 1101 40-41 -1.100992251804719 1101 42-43 -1.940033787852414 1101 44-45 -1.5828230779639796 1101 46-47 -0.8834593160762765 1101 48-49 -1.4099494247582598 1101 50-51 -1.519983745503989 1101 52-53 -0.5179335203727575 1101 54-55 -2.1874397145613003 1101 56-57 -2.0267432669026775 1101 58-59 -1.9472423992400891 1101 60-61 -1.1853506693391296 1101 62-63 -3.016622862118016 1101 64-65 -1.6732358950057957 1101 66-67 -2.092182009443608 1101 68-69 -0.9727307284131115 1101 70-71 -2.135155090308295 1101 72-73 -1.217783936436792 1101 74-75 -1.6648148808975307 1101 76 -2.9978715680853405 1102 1 -0.1952388767026889 1102 2 -1.5105097879719445 1102 3 -0.018853444707271905 1102 4 -0.9797038668240887 1102 5 -0.13584419771212097 1102 6 0.05116770419802208 1102 7 0.3678789476788715 1102 8 0.5162141386673653 1102 9 -0.31024127332408824 1102 10-11 -1.1916379147513787 1102 12-13 -3.762527907424527 1102 14-15 -1.769312622947929 1102 16-17 -3.188699379839214 1102 18-19 -0.6261146659706682 1102 20-21 -1.0671868664722872 1102 22-23 -1.3061625566804906 1102 24-25 -0.9152687347315478 1102 26-27 -5.432496914234502 1102 28-29 -0.428387287684064 1102 30-31 -0.24352225743078648 1102 32-33 -5.589722703554255 1102 34-35 -1.449986841961831 1102 36-37 -0.6475457195258514 1102 38-39 -0.9125293766044749 1102 40-41 -0.5457336146602252 1102 42-43 -0.11415098579095329 1102 44-45 -5.888950201460826 1102 46-47 -1.4225889209627027 1102 48-49 -0.6471893560428583 1102 50-51 -6.006317015211948 1102 52-53 -1.7606515963414537 1102 54-55 -0.6584074564967821 1102 56-57 -1.429606408723636 1102 58-59 -0.11746572430212154 1102 60-61 -1.2787654431509274 1102 62-63 -5.013893333582036 1102 64-65 -1.2787713025263159 1102 66-67 -0.672082753860483 1102 68-69 -6.694299730130993 1102 70-71 -0.5605034392298442 1102 72-73 -0.5174976222546945 1102 74-75 -5.52738407349154 1102 76 -2.8397879643441684 1103 1 -0.697933002516713 1103 2 -0.7209160555333476 1103 3 -0.6448823363141614 1103 4 -0.06499030363609748 1103 5 0.5225472604214936 1103 6 -1.4620384708044583 1103 7 -0.3544773380637487 1103 8 1.5147265845369233 1103 9 0.7605001897680417 1103 10-11 -0.15532506558936987 1103 12-13 -1.8342610732702838 1103 14-15 -0.654589142732398 1103 16-17 -0.43032775576067195 1103 18-19 -0.8493469558123223 1103 20-21 -0.491437304474335 1103 22-23 -0.23092537443858419 1103 24-25 -0.21937438413156585 1103 26-27 -0.9365959522828327 1103 28-29 0.007416487397751581 1103 30-31 -0.3828895510739727 1103 32-33 -4.032600624945285 1103 34-35 -1.398451355531634 1103 36-37 -0.7462697286495192 1103 38-39 0.5293568420329251 1103 40-41 -0.09774842408801376 1103 42-43 -0.08856505474735243 1103 44-45 -4.456187648156806 1103 46-47 -1.2811886366745853 1103 48-49 -0.11555403820212362 1103 50-51 0.10149760248664208 1103 52-53 -0.3022369984769213 1103 54-55 0.11812885635237791 1103 56-57 0.401423970437385 1103 58-59 -0.566326918973374 1103 60-61 0.3721551875720266 1103 62-63 -0.607576187041424 1103 64-65 0.394181657706401 1103 66-67 0.15279906822804534 1103 68-69 -2.7659006689429333 1103 70-71 0.77178309675244 1103 72-73 -0.024902336148450388 1103 74-75 -1.9782947871869538 1103 76 -0.6406540514156731 1104 1 -2.7900154529116286 1104 2 -0.39172450605668985 1104 3 0.2970684759891853 1104 4 -0.48334258001187536 1104 5 -0.2655796258560379 1104 6 0.21535188429731988 1104 7 2.3354025823112643 1104 8 1.6447593335255561 1104 9 0.2146516056801957 1104 10-11 -0.6600287193490786 1104 12-13 -0.3195400818107039 1104 14-15 -0.323808304101437 1104 16-17 -0.42936455021260755 1104 18-19 0.12975148379468848 1104 20-21 0.14562684501516543 1104 22-23 0.13770944223129433 1104 24-25 0.044688416870169334 1104 26-27 -0.385754752770854 1104 28-29 -0.7717525872827871 1104 30-31 -1.4186565837587466 1104 32-33 -1.4425248527755024 1104 34-35 -0.5556625544014686 1104 36-37 -0.7428664023796934 1104 38-39 -0.46540974115592704 1104 40-41 0.4605576184214577 1104 42-43 0.3817682143722827 1104 44-45 -2.863190152491228 1104 46-47 -0.023707740251282416 1104 48-49 0.5759793850303936 1104 50-51 0.15402441170772718 1104 52-53 -0.894110754336424 1104 54-55 -0.8767982610944856 1104 56-57 -0.09554193208621342 1104 58-59 0.27090739682962095 1104 60-61 -0.4123436244744667 1104 62-63 0.03274680788569029 1104 64-65 -0.2817638890732823 1104 66-67 0.2708880684206889 1104 68-69 -2.6075657302670194 1104 70-71 -0.8378618014688435 1104 72-73 -0.6046397021023466 1104 74-75 -1.1630536647314216 1104 76 -0.32931391142980004 1105 1 -0.4156859509959183 1105 2 0.2565513560122774 1105 3 -0.11365949335947434 1105 4 -0.20077553020344396 1105 5 0.3382517917684815 1105 6 0.10922161609808612 1105 7 0.7988125439970837 1105 8 0.9951424752880094 1105 9 0.6115864715805799 1105 10-11 1.575028751915287 1105 12-13 0.27844842393642466 1105 14-15 0.9370154506878343 1105 16-17 1.1750415800555878 1105 18-19 1.0891384569747657 1105 20-21 0.5359525155132481 1105 22-23 1.4016941165607903 1105 24-25 0.8662401410080953 1105 26-27 1.1996858602559648 1105 28-29 0.1780558418359881 1105 30-31 0.33402540857841956 1105 32-33 1.2938736146574463 1105 34-35 0.9363872540276503 1105 36-37 1.4825167393827563 1105 38-39 1.5277894925605437 1105 40-41 1.014867963249042 1105 42-43 1.1355038465561869 1105 44-45 1.7602772804589648 1105 46-47 1.2890317233502486 1105 48-49 0.5829717221951434 1105 50-51 0.4291201971483396 1105 52-53 -0.11614140567741771 1105 54-55 0.38603698794766217 1105 56-57 0.5930596004808351 1105 58-59 1.0868077799713802 1105 60-61 0.6199360690121232 1105 62-63 1.2341835894948865 1105 64-65 0.580975574528253 1105 66-67 0.771079639301913 1105 68-69 1.4823767984686143 1105 70-71 2.042981110408551 1105 72-73 0.6055135546026342 1105 74-75 -0.1403525153061338 1105 76 -0.33084647847960724 1106 1 -0.2684637287736962 1106 2 1.0357180226789424 1106 3 0.3314793955294135 1106 4 1.181168914240999 1106 5 1.555612902879595 1106 6 0.6085271716536411 1106 7 1.493951432885975 1106 8 2.22708691973245 1106 9 2.3372809160250263 1106 10-11 1.8021120852486199 1106 12-13 0.878448423936419 1106 14-15 1.1404876729100586 1106 16-17 1.361152691166705 1106 18-19 2.037402345863647 1106 20-21 1.3324802932910238 1106 22-23 1.5124580054496874 1106 24-25 1.8974901410080953 1106 26-27 1.337880304700409 1106 28-29 0.2818752862804317 1106 30-31 1.2899281863561924 1106 32-33 1.617137503546335 1106 34-35 1.5256233651387632 1106 36-37 1.5196695171605406 1106 38-39 1.0253589370049951 1106 40-41 0.8732012965823799 1106 42-43 1.4344621798895183 1106 44-45 2.0762495026811862 1106 46-47 1.6397261677946915 1106 48-49 1.9472078333062548 1106 50-51 1.1110646415927867 1106 52-53 0.5543447054336923 1106 54-55 1.438467543503215 1106 56-57 1.8364623782586094 1106 58-59 1.0854188910824902 1106 60-61 2.007088846789898 1106 62-63 1.0123085894948858 1106 64-65 2.013961685639366 1106 66-67 1.5808018615241366 1106 68-69 2.6490434651352714 1106 70-71 1.0061755548529945 1106 72-73 0.48433299904707994 1106 74-75 -0.08306084863946595 1106 76 0.5434590770759478 1107 1 -1.1318056976856141 1107 2 -0.740512547269244 1107 3 -0.6672576511027088 1107 4 -0.5166937800595193 1107 5 -0.3967510867577104 1107 6 -0.7440311185017983 1107 7 -1.4203296551969267 1107 8 -0.5078224066924193 1107 9 -1.095782555477566 1107 10-11 -0.28749283703117 1107 12-13 -0.44292463305839647 1107 14-15 -0.4481457467790584 1107 16-17 0.08537548909416515 1107 18-19 0.24123978109681588 1107 20-21 -0.22189146836700502 1107 22-23 -0.8231475644985018 1107 24-25 -0.4952883564012289 1107 26-27 -0.2271132186156528 1107 28-29 -0.8837173302998771 1107 30-31 -0.2034817877370685 1107 32-33 0.19908374706965404 1107 34-35 -0.06273479548300287 1107 36-37 -0.5180733584871291 1107 38-39 0.5037106209428188 1107 40-41 0.2172427473595775 1107 42-43 0.5029045374024754 1107 44-45 -0.3137440206348785 1107 46-47 0.047595338779146346 1107 48-49 0.2693419006637683 1107 50-51 0.14869417527154027 1107 52-53 0.8219127106150381 1107 54-55 0.11620394246694943 1107 56-57 0.6056819378441034 1107 58-59 -0.12125209337346377 1107 60-61 -0.05837423611165349 1107 62-63 -0.14522055558283142 1107 64-65 0.06112813641656345 1107 66-67 0.29045212059148895 1107 68-69 0.7271522734254319 1107 70-71 -0.3452169321936367 1107 72-73 0.2737921959382703 1107 74-75 -0.06578429423532128 1107 76 -1.1913530990898558 1108 1 -0.04937770726832014 1108 2 -0.11549165474041345 1108 3 -0.9011146904920899 1108 4 -0.08697624704932494 1108 5 -0.8173037637870735 1108 6 0.22499222541708264 1108 7 0.7807121855741457 1108 8 0.5433503605926688 1108 9 0.2248481203261008 1108 10-11 -0.28303576421374643 1108 12-13 0.5824806820009343 1108 14-15 -0.18726770343402777 1108 16-17 -0.3724494593709302 1108 18-19 -0.38433824553419527 1108 20-21 -0.24944174971972544 1108 22-23 -0.39973957519547554 1108 24-25 -0.1635985686693182 1108 26-27 -0.35479442648238546 1108 28-29 0.1015325443449484 1108 30-31 0.6268569497970518 1108 32-33 0.4988915358044004 1108 34-35 0.08755212857962036 1108 36-37 -0.15341516025881674 1108 38-39 0.3090618939942402 1108 40-41 0.033416350345817136 1108 42-43 0.6820092229002732 1108 44-45 0.3596500403155929 1108 46-47 0.8584761677946915 1108 48-49 0.26424412362883487 1108 50-51 0.11388722223794545 1108 52-53 -0.1625571762867395 1108 54-55 0.2932054467290257 1108 56-57 0.8501384535274283 1108 58-59 -1.265521969132564 1108 60-61 1.0802407285103328 1108 62-63 0.048968535731447105 1108 64-65 -0.044741271349884926 1108 66-67 0.27394702281445404 1108 68-69 0.6364090565331253 1108 70-71 1.7487158774336393 1108 72-73 0.0816784291546071 1108 74-75 0.8540023234035452 1108 76 0.3290101523447646 1109 1 0.3086639308007726 1109 2 -0.2767819773210576 1109 3 0.9592719487209038 1109 4 0.05749870147504055 1109 5 0.4397884347944867 1109 6 1.2424367461217258 1109 7 -0.6878304820076444 1109 8 1.5906507495196855 1109 9 0.8709245330463027 1109 10-11 1.3455961278018158 1109 12-13 0.0438739558513177 1109 14-15 0.009769587803678093 1109 16-17 -0.14203879819499932 1109 18-19 -0.09484499456188189 1109 20-21 0.537134548610176 1109 22-23 0.6671787501305353 1109 24-25 0.715176311220862 1109 26-27 0.3441303047004105 1109 28-29 0.4139896479825609 1109 30-31 0.6325478672072578 1109 32-33 1.1343582482271835 1109 34-35 0.36451964173450335 1109 36-37 0.46986898524564324 1109 38-39 0.619574362536909 1109 40-41 0.7157544880717381 1109 42-43 0.3395818607405836 1109 44-45 0.06601014097905633 1109 46-47 -0.04843872582232933 1109 48-49 -0.5431512092469397 1109 50-51 0.5496284713800215 1109 52-53 -0.15343454988545702 1109 54-55 -0.06798192458188979 1109 56-57 1.0048799314501018 1109 58-59 0.8888763378910021 1109 60-61 0.5817563999813871 1109 62-63 0.26556656821828994 1109 64-65 0.001129238830849033 1109 66-67 0.30234441471562334 1109 68-69 0.7245753800288952 1109 70-71 0.7097659803849119 1109 72-73 0.47389416925984307 1109 74-75 1.3140801088073424 1109 76 -0.31678028462618 1110 1 -0.6053281355533571 1110 2 -0.4660475140442202 1110 3 -0.8778779491033539 1110 4 0.24508134361952827 1110 5 -1.1026144982503503 1110 6 0.25178282137115815 1110 7 -0.044784442820240855 1110 8 0.712750761540363 1110 9 -1.0044210613761066 1110 10-11 -1.6718639034519427 1110 12-13 -0.8798849093969139 1110 14-15 -1.0414685417792064 1110 16-17 -1.0090450489462945 1110 18-19 -0.09993875583126055 1110 20-21 -1.1431906106637761 1110 22-23 -0.8848407233638724 1110 24-25 -0.5686468646416181 1110 26-27 -2.143157830892811 1110 28-29 -0.7605505611771939 1110 30-31 -1.1404567006494553 1110 32-33 -0.69661602752711 1110 34-35 -0.630326493618302 1110 36-37 -1.0675303415965232 1110 38-39 -0.7697152155373814 1110 40-41 -0.6205840141520866 1110 42-43 -1.0273492607884442 1110 44-45 -0.6563423052284207 1110 46-47 -0.9607328717533292 1110 48-49 -0.1153804435299044 1110 50-51 -0.47445795727727 1110 52-53 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0.6058936042746836 2116 42-43 0.7598628209151634 2116 44-45 1.8376277078093892 2116 46-47 0.8232197575382827 2116 48-49 0.577416166639587 2116 50-51 0.1248466928748364 2116 52-53 1.352020987484977 2116 54-55 -0.22892027700960327 2116 56-57 -0.21954723712600455 2116 58-59 -1.027722134558534 2116 60-61 -0.23241435833830337 2116 62-63 1.319439999751296 2116 64-65 0.6613174548701295 2116 66-67 0.1516351948574659 2116 68-69 0.6387870548788683 2116 70-71 -2.598471881044439 2116 72-73 0.472393896482977 2116 74-75 -1.353814053767671 2116 76 -0.7534960511291793 2117 1 1.216965719079063 2117 2 1.2661628079550162 2117 3 1.5089717268177587 2117 4 1.450724128964925 2117 5 -0.06781501123083444 2117 6 0.966004165518676 2117 7 -0.16107157324899646 2117 8 -0.5134805649301235 2117 9 2.317994106209074 2117 10-11 1.8758467478253102 2117 12-13 1.9227735773106502 2117 14-15 0.5980413538916558 2117 16-17 1.5177477831912434 2117 18-19 1.4614629286857408 2117 20-21 1.8896505386898035 2117 22-23 2.171257852075449 2117 24-25 2.875289220762699 2117 26-27 2.7873818384427373 2117 28-29 1.8237272801454587 2117 30-31 1.8182449041476048 2117 32-33 2.3339894974113626 2117 34-35 2.194584254709312 2117 36-37 0.9291595478353862 2117 38-39 1.7963136916062226 2117 40-41 0.5351644867664263 2117 42-43 0.7039598792760238 2117 44-45 2.5381743493069493 2117 46-47 1.7296418119664736 2117 48-49 1.674297557232638 2117 50-51 2.7422762980345006 2117 52-53 2.725644552059464 2117 54-55 0.6698134023989226 2117 56-57 1.9019723475837722 2117 58-59 1.8015998726775848 2117 60-61 2.5064561780782455 2117 62-63 1.5748277612740296 2117 64-65 2.069233924903166 2117 66-67 0.5721362173523517 2117 68-69 1.491528127711966 2117 70-71 2.348161751172011 2117 72-73 2.394743275120696 2117 74-75 1.2684920654709657 2117 76 0.7013961936403668 2118 1 0.19274316777802625 2118 2 -0.4319543911141608 2118 3 -0.5247706044705858 2118 4 -0.4775954535750948 2118 5 -1.179372729304312 2118 6 -0.18119983984060894 2118 7 -0.3599997165393134 2118 8 -2.774780896359502 2118 9 -1.8313254058140558 2118 10-11 -1.8209482595789659 2118 12-13 -0.7965515760635782 2118 14-15 -2.1312795684692496 2118 16-17 -1.159249607683872 2118 18-19 -1.3702485162053115 2118 20-21 -3.3716145342951798 2118 22-23 -1.2099917071939927 2118 24-25 -0.6708288245091438 2118 26-27 -0.38288118955246375 2118 28-29 -0.9470399436046257 2118 30-31 -1.2088864688162175 2118 32-33 -0.632180025189296 2118 34-35 -0.46945565784974264 2118 36-37 -1.4784985862877384 2118 38-39 -1.0717315802363885 2118 40-41 -1.6831205424980809 2118 42-43 -0.9170823603403626 2118 44-45 0.21726961762371388 2118 46-47 -0.9863514184122053 2118 48-49 -1.961376936808687 2118 50-51 -0.9350560480623891 2118 52-53 -2.0952601796237786 2118 54-55 -0.12679826109448555 2118 56-57 -0.04669135737357166 2118 58-59 -0.23771329282555342 2118 60-61 -1.679585003784812 2118 62-63 -0.40690836452809975 2118 64-65 -0.9513041189583404 2118 66-67 -2.720491241924144 2118 68-69 -0.1506691785428842 2118 70-71 -1.1826893876757403 2118 72-73 -2.5385477480793597 2118 74-75 -0.4274214808233765 2118 76 -0.6741414976366968 2119 1 0.40976207767791806 2119 2 0.06837931300152178 2119 3 0.07200358907780213 2119 4 0.758185043273258 2119 5 -0.10870161324943695 2119 6 1.3067126555246134 2119 7 -1.8719759864688648 2119 8 1.0928127261840643 2119 9 -0.6439690839749765 2119 10-11 -0.22712178571912034 2119 12-13 0.7760290690977101 2119 14-15 -0.5947945851544567 2119 16-17 0.23937849761831487 2119 18-19 -0.0703597509105407 2119 20-21 -0.9225600292896168 2119 22-23 0.5792926828690383 2119 24-25 -0.011985665443514648 2119 26-27 0.4650980466358945 2119 28-29 0.6354035120868851 2119 30-31 0.12631931538845365 2119 32-33 0.9714721809656943 2119 34-35 0.527337074816181 2119 36-37 -0.013092579613655175 2119 38-39 0.32787909829531614 2119 40-41 -0.4719599937402066 2119 42-43 -0.44971120720725466 2119 44-45 -0.8215327553833305 2119 46-47 0.026218103278559113 2119 48-49 -0.13898168282277723 2119 50-51 1.1767904480443967 2119 52-53 -0.3792238429534045 2119 54-55 -1.2196977790774284 2119 56-57 0.711966410516677 2119 58-59 1.323725342695397 2119 60-61 0.07916545969312949 2119 62-63 -0.11017124921479038 2119 64-65 0.17837700822001068 2119 66-67 0.21426960345961987 2119 68-69 -0.8436984703485919 2119 70-71 -0.3835421870824902 2119 72-73 -1.1887516783722774 2119 74-75 -0.05513746154269228 2119 76 0.9827735932049819 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 5.0 12 21.0 13 133.0 14 518.0 15 1038.0 16 1661.0 17 2114.0 18 2182.0 19 2207.0 20 1905.0 21 1591.0 22 1169.0 23 925.0 24 871.0 25 890.0 26 987.0 27 1100.0 28 1239.0 29 1477.0 30 1680.0 31 2124.0 32 2680.0 33 3511.0 34 4500.0 35 6764.0 36 10671.0 37 11001.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.22377091117692 21.961580799702322 10.015659157506319 34.79898913161444 2 16.658200569537442 22.182713768952514 31.410759639806052 29.748326021703996 3 15.177403217116908 24.39929192642192 28.10590317863465 32.317401677826524 4 11.27068421457708 24.41006695913184 33.34872623720465 30.97052258908643 5 10.57646424998076 39.10567228507658 32.03263295620719 18.285230508735474 6 21.643962133456476 36.08558454552451 16.612021857923498 25.658431463095514 7 20.9959208804741 30.57030708843223 19.936889094127608 28.496882936966056 8 18.959439698299086 34.59709074116832 21.092896174863387 25.350573385669207 9 20.664973447240822 20.115446779034865 20.084660971292234 39.134918802432075 10-11 20.641884091433848 26.32032632956207 26.77364734857231 26.26414223043177 12-13 20.219348880166244 24.71561610097745 25.657661817901946 29.40737320095436 14-15 19.8583852843839 25.949357346263373 22.900792734549373 31.291464634803358 16-17 21.148310628800125 28.2352035711537 23.98522281228354 26.631262987762643 18-19 19.637497113830523 27.145385977064574 24.553220965135072 28.66389594396983 20-21 19.145693835142 27.214654044485492 25.005002693758176 28.634649426614327 22-23 21.8232894635573 27.08227507119218 24.176094820287847 26.918340644962672 24-25 20.486415762333564 28.810128530747324 24.05910875086585 26.644346956053262 26-27 18.73470330177788 29.596705918571537 25.51142923112445 26.15716154852613 28-29 20.374817209266528 27.38705456784422 27.68952512891557 24.54860309397368 30-31 20.46024782575233 27.266989917647965 27.20080043100131 25.0719618255984 32-33 18.643885168937118 29.501269914569384 26.568921727083815 25.285923189409683 34-35 19.939967674901872 28.780112368198264 25.833910567228507 25.44600938967136 36-37 20.46717463249442 27.348572308165934 26.290310167013008 25.89394289232664 38-39 19.92303548064342 26.735934734087586 27.61563919033326 25.72539059493573 40-41 19.400446394212267 26.439621334564766 27.37474024474717 26.785192026475794 42-43 18.279073347186948 26.18563842068806 28.24213037789579 27.2931578542292 44-45 18.089740629569768 26.95682290464096 28.663126298776266 26.290310167013008 46-47 18.252135765412145 27.44169937658739 27.91118294466251 26.39498191333795 48-49 17.858077426306473 28.47995074270761 27.04456245670746 26.617409374278456 50-51 17.808820133918264 27.64796428846302 26.739782960055415 27.8034326175633 52-53 17.677980451012086 27.65489109520511 26.995305164319248 27.67182328946356 54-55 17.205418302162702 27.878088201339185 26.625105826214114 28.291387670284003 56-57 18.26368044331563 27.42784576310321 26.30108519972293 28.00738859385823 58-59 18.209035634572462 27.401677826521976 26.179481259139536 28.209805279766027 60-61 17.25159701377665 26.58431463095513 26.41499268837066 29.749095666897563 62-63 17.766489648272145 26.93142461325329 25.897791118294467 29.4042946201801 64-65 18.27676441160625 27.32086508119757 24.752559070268607 29.649811436927575 66-67 17.742630647271607 28.456861386900638 24.07219271915647 29.728315246671283 68-69 18.243669668282923 28.452243515739244 24.400061571615485 28.904025244362348 70-71 19.161234212531458 27.61816068776024 24.43180506276505 28.78880003694325 72-73 19.055645347494803 26.85522973909028 24.636342646040173 29.45278226737474 74-75 19.39505887785731 26.5512198876318 24.72100361733241 29.33271761717848 76 20.189332717617177 24.83183252520588 25.599938428384515 29.378896328792425 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.5 12 1.0 13 1.0 14 4.0 15 7.5 16 8.5 17 9.0 18 11.5 19 16.5 20 26.5 21 34.0 22 41.5 23 56.0 24 77.5 25 92.0 26 124.0 27 183.5 28 280.0 29 349.0 30 359.5 31 510.0 32 730.5 33 811.0 34 914.5 35 1252.5 36 1668.5 37 1850.0 38 2047.5 39 2539.5 40 3126.0 41 3579.0 42 3740.0 43 3944.0 44 4328.0 45 4503.0 46 4498.0 47 4504.0 48 4424.0 49 4204.0 50 4070.0 51 3813.5 52 3264.5 53 2771.5 54 2571.0 55 2427.0 56 2061.5 57 1671.5 58 1503.0 59 1295.0 60 997.5 61 805.0 62 702.0 63 614.5 64 463.0 65 363.0 66 267.5 67 208.0 68 191.5 69 147.5 70 93.0 71 66.0 72 54.0 73 42.5 74 34.5 75 26.0 76 18.0 77 11.0 78 10.0 79 8.0 80 7.5 81 8.5 82 8.0 83 6.0 84 7.5 85 6.0 86 2.5 87 2.0 88 3.0 89 2.5 90 1.5 91 1.5 92 1.0 93 2.5 94 5.5 95 4.0 96 1.0 97 2.0 98 3.5 99 4.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7173093204032941 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 7.696451935657662E-4 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 64965.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.11729392749943 #Duplication Level Percentage of deduplicated Percentage of total 1 83.37282021728458 60.126221811744784 2 8.379757102303046 12.086508119756791 3 3.6349277496745 7.864234587854998 4 1.7865146955241082 5.15354421611637 5 0.9049966916394527 3.2632956207188486 6 0.6061770293056712 2.622950819672131 7 0.4012721179910781 2.0257061494650968 8 0.3030885146528356 1.7486338797814207 9 0.18782950203837698 1.2191179866081736 >10 0.4226163795863482 3.8897868082813822 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0061571615485261294 0.0 2 0.0 0.0 0.0 0.0061571615485261294 0.0 3 0.0 0.0 0.0 0.0061571615485261294 0.0 4 0.0 0.0 0.0 0.0061571615485261294 0.0 5 0.0 0.0 0.0 0.007696451935657662 0.0 6 0.0 0.0 0.0 0.007696451935657662 0.0 7 0.0 0.0 0.0 0.007696451935657662 0.0 8 0.0 0.0 0.0 0.007696451935657662 0.0 9 0.0 0.0 0.0 0.009235742322789195 0.0 10 0.0 0.0 0.0 0.009235742322789195 0.0 11 0.0 0.0 0.0 0.012314323097052259 0.0 12 0.0 0.0 0.0 0.012314323097052259 0.0 13 0.0 0.0 0.0 0.013853613484183791 0.0 14 0.0 0.0 0.0 0.013853613484183791 0.0 15 0.0 0.0 0.0 0.013853613484183791 0.0 16 0.0 0.0 0.0 0.016932194258446855 0.0 17 0.0 0.0 0.0 0.01847148464557839 0.0 18 0.0 0.0 0.0 0.021550065419841453 0.0 19 0.0 0.0 0.0 0.030785807742630646 0.0 20 0.0 0.0 0.0 0.030785807742630646 0.0 21 0.0 0.0 0.0 0.030785807742630646 0.0 22 0.0 0.0 0.0 0.030785807742630646 0.0 23 0.0 0.0 0.0 0.035403678904025244 0.0 24 0.0 0.0 0.0 0.035403678904025244 0.0 25 0.0 0.0 0.0 0.03694296929115678 0.0 26 0.0 0.0 0.0 0.04310013083968291 0.0 27 0.0 0.0 0.0 0.055414453936735164 0.0 28 0.0 0.0 0.0 0.07080735780805049 0.0 29 0.0 0.0 0.0 0.095436004002155 0.0 30 0.0 0.0 0.0 0.1369968444547064 0.0 31 0.0 0.0 0.0 0.16470407142307397 0.0 32 0.0 0.0 0.0 0.20626491187562535 0.0 33 0.0 0.0 0.0 0.2586007850380974 0.0 34 0.0 0.0 0.0 0.32940814284614794 0.0 35 0.0 0.0 0.0 0.427922727622566 0.0 36 0.0 0.0 0.0 0.5618409913030094 0.0 37 0.0 0.0 0.0 0.852766874470869 0.0 38 0.0 0.0 0.0 1.115985530670361 0.0 39 0.0 0.0 0.0 1.4715616100977449 0.0 40 0.0 0.0 0.0 1.8317555606865235 0.0 41 0.0 0.0 0.0 2.267374740244747 0.0 42 0.0 0.0 0.0 2.723004694835681 0.0 43 0.0 0.0 0.0 3.2402062649118757 0.0 44 0.0 0.0 0.0 3.7574078349880704 0.0 45 0.0 0.0 0.0 4.222273531901793 0.0 46 0.0 0.0 0.0 4.722542907719541 0.0 47 0.0 0.0 0.0 5.230508735472947 0.0 48 0.0 0.0 0.0 5.761563919033326 0.0 49 0.0 0.0 0.0 6.254136842915416 0.0 50 0.0 0.0 0.0 6.768259832217348 0.0 51 0.0 0.0 0.0 7.303932886939121 0.0 52 0.0 0.0 0.0 7.758023551142923 0.0 53 0.0 0.0 0.0 8.319864542445933 0.0 54 0.0 0.0 0.0 8.84014469329639 0.0 55 0.0 0.0 0.0 9.286538905564536 0.0 56 0.0 0.0 0.0 9.7437081505426 0.0 57 0.0 0.0 0.0 10.139305780035404 0.0 58 0.0 0.0 0.0 10.550296313399523 0.0 59 0.0 0.0 0.0 10.918186715923959 0.0 60 0.0 0.0 0.0 11.364580928192103 0.0 61 0.0 0.0 0.0 11.798660817363196 0.0 62 0.0 0.0 0.0 12.237358577695682 0.0 63 0.0 0.0 0.0 12.674517047641038 0.0 64 0.0 0.0 0.0 13.110136227199261 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGGT 15 0.0022165026 69.99154 63 GTCATTC 20 0.0069260034 52.49365 30 GGTCGGA 20 0.0069260034 52.49365 25 TATGGAC 20 0.0069260034 52.49365 6 CTCTTCG 20 0.0069260034 52.49365 39 TGGCCAC 20 0.0069260034 52.49365 16 GAGACAA 20 0.0069260034 52.49365 12 CGAGGCA 20 0.0069260034 52.49365 24 GATATGG 20 0.0069260034 52.49365 4 GACCTCA 20 0.0069260034 52.49365 67 GTCGGAG 20 0.0069260034 52.49365 26 AGGGGGG 30 5.841785E-4 46.661022 9 TGATATG 35 0.001247785 39.99516 3 AACCCAT 35 0.001247785 39.99516 4 TCTTTCA 35 0.001247785 39.99516 8 AGGAGAC 45 0.0042814184 31.10735 10 CATCGCT 45 0.0042814184 31.10735 37 GAGGGGG 60 4.9368077E-4 29.16314 8 CCATCGC 50 0.0071654026 27.996614 36 CCAAGAC 50 0.0071654026 27.996614 63 >>END_MODULE